Restriction map for
pF1KB5212
(Circular) (Six-base) MAPSORT of: pF1KB5212.seq Check: 8002 from: 1 to: 4295
pF1KB5212 4295 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1207 1207
Size: 4295
AccI GT'mk_AC
Cuts at: 215 1333 215
Size: 1118 3177
Acc65I G'GTAC_C
Cuts at: 1311 1311
Size: 4295
AclI AA'CG_TT
Cuts at: 4135 4135
Size: 4295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2120 2552 2905 3567 2120
Size: 432 353 662 2848
Fragments arranged by size:
2848 662 432 353
AflIII A'CryG_T
Cuts at: 767 1104 1647 3447 3728 767
Size: 337 543 1800 281 1334
Fragments arranged by size:
1800 1334 543 337 281
AleI CACnn'nnGTG
Cuts at: 274 274
Size: 4295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 463 463
Size: 4295
AlwNI CAG_nnn'CTG
Cuts at: 696 1600 3038 696
Size: 904 1438 1953
Fragments arranged by size:
1953 1438 904
ApaLI G'TGCA_C
Cuts at: 3133 3133
Size: 4295
ApoI r'AATT_y
Cuts at: 541 1048 1299 3648 3904 541
Size: 507 251 2349 256 932
Fragments arranged by size:
2349 932 507 256 251
AseI AT'TA_AT
Cuts at: 20 20
Size: 4295
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
AvaI C'yCGr_G
Cuts at: 118 472 1084 1112 1315 118
Size: 354 612 28 203 3098
Fragments arranged by size:
3098 612 354 203 28
BamHI G'GATC_C
Cuts at: 290 809 1320 290
Size: 519 511 3265
Fragments arranged by size:
3265 519 511
BanI G'GyrC_C
Cuts at: 447 1311 1725 1973 2008 447
Size: 864 414 248 35 2734
Fragments arranged by size:
2734 864 414 248 35
BanII G_rGCy'C
Cuts at: 1091 1309 2339 1091
Size: 218 1030 3047
Fragments arranged by size:
3047 1030 218
BbeI G_GCGC'C
Cuts at: 1729 1977 1729
Size: 248 4047
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 814 848 814
Size: 34 4261
BciVI GTATCCnnnnn_n'
Cuts at: 2193 3249 4295 2193
Size: 1056 1046 2193
Fragments arranged by size:
2193 1056 1046
BclI T'GATC_A
Cuts at: 659 1815 659
Size: 1156 3139
BglII A'GATC_T
Cuts at: 990 1810 3847 990
Size: 820 2037 1438
Fragments arranged by size:
2037 1438 820
BlpI GC'TnA_GC
Cuts at: 1407 1407
Size: 4295
Bme1580I G_kGCm'C
Cuts at: 452 633 1920 2013 3137 452
Size: 181 1287 93 1124 1610
Fragments arranged by size:
1610 1287 1124 181 93
BmgBI CAC'GTC
Cuts at: 657 777 657
Size: 120 4175
BmrI ACTGGGnnnn_n'
Cuts at: 627 1507 1588 1923 4067 627
Size: 880 81 335 2144 855
Fragments arranged by size:
2144 880 855 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 4295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 272
Size: 4295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1471 2350 3064 3362 1471
Size: 879 714 298 2404
Fragments arranged by size:
2404 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 727 3999 727
Size: 3272 1023
BsaAI yAC'GTr
Cuts at: 1648 1648
Size: 4295
BsaBI GATnn'nnATC
Cuts at: 6 289 1809 3846 6
Size: 283 1520 2037 455
Fragments arranged by size:
2037 1520 455 283
BsaHI Gr'CG_yC
Cuts at: 1173 1726 1974 2676 4172 1173
Size: 553 248 702 1496 1296
Fragments arranged by size:
1496 1296 702 553 248
BsaWI w'CCGG_w
Cuts at: 1484 2005 2683 3094 3241 3515 1484
Size: 521 678 411 147 274 2264
Fragments arranged by size:
2264 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 586 616 586
Size: 30 4265
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 166
Size: 4295
BseYI C'CCAG_C
Cuts at: 243 1015 1720 2478 3143 3551 243
Size: 772 705 758 665 408 987
Fragments arranged by size:
987 772 758 705 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 403
Size: 4295
BsiEI CG_ry'CG
Cuts at: 104 1837 1883 3113 104
Size: 1733 46 1230 1286
Fragments arranged by size:
1733 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1309 2087 2277 3137 1309
Size: 778 190 860 2467
Fragments arranged by size:
2467 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 452 633 1091 1309 1920 2013 2087 2277 2339
Size: 181 458 218 611 93 74 190 62
Cuts at: 2339 3137 452
Size: 798 1610
Fragments arranged by size:
1610 798 611 458 218 190 181 93
74 62
BspHI T'CATG_A
Cuts at: 2727 2727
Size: 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 201 704 1207 1345 1559 1861 2242 201
Size: 503 503 138 214 302 381 2254
Fragments arranged by size:
2254 503 503 381 302 214 138
BsrBI CCG'CTC
Cuts at: 50 2587 2641 4125 4290 50
Size: 2537 54 1484 165 55
Fragments arranged by size:
2537 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2207 2207
Size: 4295
BsrFI r'CCGG_y
Cuts at: 2293 2474 2293
Size: 181 4114
BssSI C'ACGA_G
Cuts at: 2566 3274 2566
Size: 708 3587
BstAPI GCAn_nnn'nTGC
Cuts at: 234 993 1672 234
Size: 759 679 2857
Fragments arranged by size:
2857 759 679
BstBI TT'CG_AA
Cuts at: 2656 2656
Size: 4295
BstYI r'GATC_y
Cuts at: 1 290 809 990 1320 1787 1810 2145 2391
Size: 289 519 181 330 467 23 335 246
Cuts at: 2391 2697 2709 2795 3847 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 519 467 449 335 330 306 289
246 181 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1070 1070
Size: 4295
BtgI C'CryG_G
Cuts at: 107 455 107
Size: 348 3947
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 374 806 2245 2426 2589 374
Size: 432 1439 181 163 2080
Fragments arranged by size:
2080 1439 432 181 163
BtsI GCAGTG_nn'
Cuts at: 1154 1538 1154
Size: 384 3911
DraI TTT'AAA
Cuts at: 1294 1294
Size: 4295
DrdI GACnn_nn'nnGTC
Cuts at: 211 2001 3345 211
Size: 1790 1344 1161
Fragments arranged by size:
1790 1344 1161
EaeI y'GGCC_r
Cuts at: 428 464 783 851 1880 2054 2445 2472 3817
Size: 36 319 68 1029 174 391 27 1345
Cuts at: 3817 428
Size: 906
Fragments arranged by size:
1345 1029 906 391 319 174 68 36
27
EagI C'GGCC_G
Cuts at: 1880 1880
Size: 4295
EarI CTCTTCn'nnn_
Cuts at: 323 928 2318 2528 323
Size: 605 1390 210 2090
Fragments arranged by size:
2090 1390 605 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1213 2503 3245 3391 4105 1213
Size: 1290 742 146 714 1403
Fragments arranged by size:
1403 1290 742 714 146
EcoICRI GAG'CTC
Cuts at: 1307 1307
Size: 4295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 2120 2552 2905 3567 272
Size: 1848 432 353 662 1000
Fragments arranged by size:
1848 1000 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 406 907 406
Size: 501 3794
EcoO109I rG'GnC_Cy
Cuts at: 333 693 1098 1434 333
Size: 360 405 336 3194
Fragments arranged by size:
3194 405 360 336
EcoRI G'AATT_C
Cuts at: 541 1299 541
Size: 758 3537
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 4295
FspI TGC'GCA
Cuts at: 955 2076 955
Size: 1121 3174
HaeII r_GCGC'y
Cuts at: 1729 1977 3207 3978 1729
Size: 248 1230 771 2046
Fragments arranged by size:
2046 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2137 2167 2169 2199 2137
Size: 30 2 30 4233
Fragments arranged by size:
4233 30 30 2
HincII GTy'rAC
Cuts at: 759 1334 759
Size: 575 3720
KasI G'GCGC_C
Cuts at: 1725 1973 1725
Size: 248 4047
KpnI G_GTAC'C
Cuts at: 1315 1315
Size: 4295
MluI A'CGCG_T
Cuts at: 1104 3728 1104
Size: 2624 1671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 629 3054 3238 629
Size: 2425 184 1686
Fragments arranged by size:
2425 1686 184
MscI TGG'CCA
Cuts at: 466 785 853 2056 466
Size: 319 68 1203 2705
Fragments arranged by size:
2705 1203 319 68
MslI CAynn'nnrTG
Cuts at: 274 302 844 964 2284 2411 274
Size: 28 542 120 1320 127 2158
Fragments arranged by size:
2158 1320 542 127 120 28
MspA1I CmG'CkG
Cuts at: 195 618 1406 1720 2080 2864 3109 195
Size: 423 788 314 360 784 245 1381
Fragments arranged by size:
1381 788 784 423 360 314 245
NaeI GCC'GGC
Cuts at: 2476 2476
Size: 4295
NarI GG'CG_CC
Cuts at: 1726 1974 1726
Size: 248 4047
NcoI C'CATG_G
Cuts at: 107 107
Size: 4295
NgoMIV G'CCGG_C
Cuts at: 2474 2474
Size: 4295
NspI r_CATG'y
Cuts at: 301 556 771 1266 1348 1848 3451 301
Size: 255 215 495 82 500 1603 1145
Fragments arranged by size:
1603 1145 500 495 255 215 82
PciI A'CATG_T
Cuts at: 767 3447 767
Size: 2680 1615
PfoI T'CCnGG_A
Cuts at: 641 878 641
Size: 237 4058
PmeI GTTT'AAAC
Cuts at: 1294 1294
Size: 4295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 466 498 724 756 2741 2773 466
Size: 32 226 32 1985 32 1988
Fragments arranged by size:
1988 1985 226 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 333 693 1098 333
Size: 360 405 3530
Fragments arranged by size:
3530 405 360
PstI C_TGCA'G
Cuts at: 200 908 1342 2027 200
Size: 708 434 685 2468
Fragments arranged by size:
2468 708 685 434
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4295
PvuII CAG'CTG
Cuts at: 195 618 1720 2080 195
Size: 423 1102 360 2410
Fragments arranged by size:
2410 1102 423 360
RsrII CG'GwC_CG
Cuts at: 2490 2490
Size: 4295
SacI G_AGCT'C
Cuts at: 1309 1309
Size: 4295
SalI G'TCGA_C
Cuts at: 1332 1332
Size: 4295
SapI GCTCTTCn'nnn_
Cuts at: 2318 2528 2318
Size: 210 4085
SbfI CC_TGCA'GG
Cuts at: 908 1342 908
Size: 434 3861
ScaI AGT'ACT
Cuts at: 637 1123 3812 637
Size: 486 2689 1120
Fragments arranged by size:
2689 1120 486
SexAI A'CCwGG_T
Cuts at: 443 443
Size: 4295
SfcI C'TryA_G
Cuts at: 33 196 904 1338 2023 2991 3182 33
Size: 163 708 434 685 968 191 1146
Fragments arranged by size:
1146 968 708 685 434 191 163
SfoI GGC'GCC
Cuts at: 1727 1975 1727
Size: 248 4047
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
SmaI CCC'GGG
Cuts at: 1114 1317 1114
Size: 203 4092
SmlI C'TyrA_G
Cuts at: 1450 2365 3079 3341 1450
Size: 915 714 262 2404
Fragments arranged by size:
2404 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4295
SphI G_CATG'C
Cuts at: 556 1266 1348 1848 556
Size: 710 82 500 3003
Fragments arranged by size:
3003 710 500 82
StyI C'CwwG_G
Cuts at: 107 557 1429 1783 107
Size: 450 872 354 2619
Fragments arranged by size:
2619 872 450 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 148 235 769 1878 2011 2470 2679 148
Size: 87 534 1109 133 459 209 1764
Fragments arranged by size:
1764 1109 534 459 209 133 87
TatI w'GTAC_w
Cuts at: 635 1121 3810 635
Size: 486 2689 1120
Fragments arranged by size:
2689 1120 486
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4263
Tth111I GACn'n_nGTC
Cuts at: 754 2092 754
Size: 1338 2957
XbaI T'CTAG_A
Cuts at: 1326 1326
Size: 4295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 441 744 843 1790 441
Size: 303 99 947 2946
Fragments arranged by size:
2946 947 303 99
XmaI C'CCGG_G
Cuts at: 1112 1315 1112
Size: 203 4092
XmnI GAAnn'nnTTC
Cuts at: 481 922 481
Size: 441 3854
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmtI Bpu10I
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI