Restriction map for
pF1KB5221
(Circular) (Six-base) MAPSORT of: pF1KB5221.seq Check: 7335 from: 1 to: 4109
pF1KB5221 4109 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 795 795
Size: 4109
AccI GT'mk_AC
Cuts at: 1147 1147
Size: 4109
Acc65I G'GTAC_C
Cuts at: 1125 1125
Size: 4109
AclI AA'CG_TT
Cuts at: 3949 3949
Size: 4109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 1934 2366 2719 3381 760
Size: 1174 432 353 662 1488
Fragments arranged by size:
1488 1174 662 432 353
AflIII A'CryG_T
Cuts at: 1461 3261 3542 1461
Size: 1800 281 2028
Fragments arranged by size:
2028 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1414 2852 1414
Size: 1438 2671
ApaLI G'TGCA_C
Cuts at: 916 2947 916
Size: 2031 2078
ApoI r'AATT_y
Cuts at: 460 1113 3462 3718 460
Size: 653 2349 256 851
Fragments arranged by size:
2349 851 653 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4109
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4109
AvaI C'yCGr_G
Cuts at: 1129 1129
Size: 4109
BamHI G'GATC_C
Cuts at: 1082 1134 1082
Size: 52 4057
BanI G'GyrC_C
Cuts at: 1125 1539 1787 1822 1125
Size: 414 248 35 3412
Fragments arranged by size:
3412 414 248 35
BanII G_rGCy'C
Cuts at: 1123 2153 1123
Size: 1030 3079
BbeI G_GCGC'C
Cuts at: 1543 1791 1543
Size: 248 3861
BbsI GAAGACnn'nnnn_
Cuts at: 278 748 960 278
Size: 470 212 3427
Fragments arranged by size:
3427 470 212
BciVI GTATCCnnnnn_n'
Cuts at: 2007 3063 4109 2007
Size: 1056 1046 2007
Fragments arranged by size:
2007 1056 1046
BclI T'GATC_A
Cuts at: 830 1629 830
Size: 799 3310
BglII A'GATC_T
Cuts at: 1624 3661 1624
Size: 2037 2072
BlpI GC'TnA_GC
Cuts at: 1221 1221
Size: 4109
Bme1580I G_kGCm'C
Cuts at: 402 920 1734 1827 2951 402
Size: 518 814 93 1124 1560
Fragments arranged by size:
1560 1124 814 518 93
BmgBI CAC'GTC
Cuts at: 403 403
Size: 4109
BmrI ACTGGGnnnn_n'
Cuts at: 916 1321 1402 1737 3881 916
Size: 405 81 335 2144 1144
Fragments arranged by size:
2144 1144 405 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2476 2476
Size: 4109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1285 2164 2878 3176 1285
Size: 879 714 298 2218
Fragments arranged by size:
2218 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3813 3813
Size: 4109
BsaAI yAC'GTr
Cuts at: 543 1462 543
Size: 919 3190
BsaBI GATnn'nnATC
Cuts at: 6 1623 3660 6
Size: 1617 2037 455
Fragments arranged by size:
2037 1617 455
BsaHI Gr'CG_yC
Cuts at: 1540 1788 2490 3986 1540
Size: 248 702 1496 1663
Fragments arranged by size:
1663 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1298 1819 2497 2908 3055 3329 1298
Size: 521 678 411 147 274 2078
Fragments arranged by size:
2078 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 394 639 1534 2292 2957 3365 394
Size: 245 895 758 665 408 1138
Fragments arranged by size:
1138 895 758 665 408 245
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 1085 733
Size: 352 3757
BsiEI CG_ry'CG
Cuts at: 104 1651 1697 2927 104
Size: 1547 46 1230 1286
Fragments arranged by size:
1547 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 920 1123 1901 2091 2951 920
Size: 203 778 190 860 2078
Fragments arranged by size:
2078 860 778 203 190
Bsp1286I G_dGCh'C
Cuts at: 402 920 1123 1734 1827 1901 2091 2153 2951
Size: 518 203 611 93 74 190 62 798
Cuts at: 2951 402
Size: 1560
Fragments arranged by size:
1560 798 611 518 203 190 93 74
62
BspHI T'CATG_A
Cuts at: 2541 2541
Size: 4109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 795 1159 1373 1675 2056 795
Size: 364 214 302 381 2848
Fragments arranged by size:
2848 381 364 302 214
BsrBI CCG'CTC
Cuts at: 50 2401 2455 3939 4104 50
Size: 2351 54 1484 165 55
Fragments arranged by size:
2351 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2021 2021
Size: 4109
BsrFI r'CCGG_y
Cuts at: 2107 2288 2107
Size: 181 3928
BssSI C'ACGA_G
Cuts at: 2380 3088 2380
Size: 708 3401
BstAPI GCAn_nnn'nTGC
Cuts at: 1486 1486
Size: 4109
BstBI TT'CG_AA
Cuts at: 2470 2470
Size: 4109
BstYI r'GATC_y
Cuts at: 1 297 1082 1134 1601 1624 1959 2205 2511
Size: 296 785 52 467 23 335 246 306
Cuts at: 2511 2523 2609 3661 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 785 467 449 335 306 296 246
86 52 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2059 2240 2403 2059
Size: 181 163 3765
Fragments arranged by size:
3765 181 163
BtsI GCAGTG_nn'
Cuts at: 1352 1352
Size: 4109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 506 541 506
Size: 35 4074
DraI TTT'AAA
Cuts at: 459 1108 459
Size: 649 3460
DrdI GACnn_nn'nnGTC
Cuts at: 1815 3159 1815
Size: 1344 2765
EaeI y'GGCC_r
Cuts at: 1694 1868 2259 2286 3631 1694
Size: 174 391 27 1345 2172
Fragments arranged by size:
2172 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1694 1694
Size: 4109
EarI CTCTTCn'nnn_
Cuts at: 1037 1054 2132 2342 1037
Size: 17 1078 210 2804
Fragments arranged by size:
2804 1078 210 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2317 3059 3205 3919 2317
Size: 742 146 714 2507
Fragments arranged by size:
2507 742 714 146
EcoICRI GAG'CTC
Cuts at: 1121 1121
Size: 4109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 1934 2366 2719 3381 760
Size: 1174 432 353 662 1488
Fragments arranged by size:
1488 1174 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 1018 1018
Size: 4109
EcoO109I rG'GnC_Cy
Cuts at: 672 1248 672
Size: 576 3533
EcoRI G'AATT_C
Cuts at: 1113 1113
Size: 4109
EcoRV GAT'ATC
Cuts at: 531 531
Size: 4109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 4109
FspI TGC'GCA
Cuts at: 1890 1890
Size: 4109
HaeII r_GCGC'y
Cuts at: 1543 1791 3021 3792 1543
Size: 248 1230 771 1860
Fragments arranged by size:
1860 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 123 155 282 314 927 959 1951 1981 1983
Size: 32 127 32 613 32 992 30 2
Cuts at: 1983 2013 123
Size: 30 2219
Fragments arranged by size:
2219 992 613 127 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1148 1148
Size: 4109
KasI G'GCGC_C
Cuts at: 1539 1787 1539
Size: 248 3861
KpnI G_GTAC'C
Cuts at: 1129 1129
Size: 4109
MluI A'CGCG_T
Cuts at: 3542 3542
Size: 4109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 327 2868 3052 327
Size: 2541 184 1384
Fragments arranged by size:
2541 1384 184
MscI TGG'CCA
Cuts at: 1870 1870
Size: 4109
MslI CAynn'nnrTG
Cuts at: 2098 2225 2098
Size: 127 3982
MspA1I CmG'CkG
Cuts at: 129 1220 1534 1894 2678 2923 129
Size: 1091 314 360 784 245 1315
Fragments arranged by size:
1315 1091 784 360 314 245
NaeI GCC'GGC
Cuts at: 2290 2290
Size: 4109
NarI GG'CG_CC
Cuts at: 1540 1788 1540
Size: 248 3861
NcoI C'CATG_G
Cuts at: 107 107
Size: 4109
NgoMIV G'CCGG_C
Cuts at: 2288 2288
Size: 4109
NspI r_CATG'y
Cuts at: 1162 1662 3265 1162
Size: 500 1603 2006
Fragments arranged by size:
2006 1603 500
PciI A'CATG_T
Cuts at: 3261 3261
Size: 4109
PmeI GTTT'AAAC
Cuts at: 1108 1108
Size: 4109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2555 2587 2555
Size: 32 4077
PpuMI rG'GwC_Cy
Cuts at: 672 672
Size: 4109
PsiI TTA'TAA
Cuts at: 137 137
Size: 4109
PstI C_TGCA'G
Cuts at: 1156 1841 1156
Size: 685 3424
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4109
PvuII CAG'CTG
Cuts at: 129 1534 1894 129
Size: 1405 360 2344
Fragments arranged by size:
2344 1405 360
RsrII CG'GwC_CG
Cuts at: 2304 2304
Size: 4109
SacI G_AGCT'C
Cuts at: 1123 1123
Size: 4109
SalI G'TCGA_C
Cuts at: 1146 1146
Size: 4109
SapI GCTCTTCn'nnn_
Cuts at: 2132 2342 2132
Size: 210 3899
SbfI CC_TGCA'GG
Cuts at: 1156 1156
Size: 4109
ScaI AGT'ACT
Cuts at: 3626 3626
Size: 4109
SfcI C'TryA_G
Cuts at: 33 872 1152 1837 2805 2996 33
Size: 839 280 685 968 191 1146
Fragments arranged by size:
1146 968 839 685 280 191
SfoI GGC'GCC
Cuts at: 1541 1789 1541
Size: 248 3861
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4109
SmaI CCC'GGG
Cuts at: 1131 1131
Size: 4109
SmlI C'TyrA_G
Cuts at: 1264 2179 2893 3155 1264
Size: 915 714 262 2218
Fragments arranged by size:
2218 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4109
SphI G_CATG'C
Cuts at: 1162 1662 1162
Size: 500 3609
StyI C'CwwG_G
Cuts at: 107 675 1243 1597 107
Size: 568 568 354 2619
Fragments arranged by size:
2619 568 568 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 656 665 767 935 1692 1825 2284 2493 656
Size: 9 102 168 757 133 459 209 2272
Fragments arranged by size:
2272 757 459 209 168 133 102 9
TatI w'GTAC_w
Cuts at: 3624 3624
Size: 4109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 543 575 910 942 3626 3658 543
Size: 32 335 32 2684 32 994
Fragments arranged by size:
2684 994 335 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1906 1906
Size: 4109
XbaI T'CTAG_A
Cuts at: 1140 1140
Size: 4109
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1604 1604
Size: 4109
XmaI C'CCGG_G
Cuts at: 1129 1129
Size: 4109
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
DraIII FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI