Restriction map for
pF1KB5224
(Circular) (Six-base) MAPSORT of: pF1KB5224.seq Check: 4312 from: 1 to: 3836
pF1KB5224 3836 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 874 874
Size: 3836
Acc65I G'GTAC_C
Cuts at: 852 852
Size: 3836
AclI AA'CG_TT
Cuts at: 3676 3676
Size: 3836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1661 2093 2446 3108 1661
Size: 432 353 662 2389
Fragments arranged by size:
2389 662 432 353
AflIII A'CryG_T
Cuts at: 1188 2988 3269 1188
Size: 1800 281 1755
Fragments arranged by size:
1800 1755 281
AleI CACnn'nnGTG
Cuts at: 766 799 766
Size: 33 3803
AlwNI CAG_nnn'CTG
Cuts at: 472 648 1141 2579 472
Size: 176 493 1438 1729
Fragments arranged by size:
1729 1438 493 176
ApaLI G'TGCA_C
Cuts at: 2674 2674
Size: 3836
ApoI r'AATT_y
Cuts at: 771 840 3189 3445 771
Size: 69 2349 256 1162
Fragments arranged by size:
2349 1162 256 69
AseI AT'TA_AT
Cuts at: 20 20
Size: 3836
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
AvaI C'yCGr_G
Cuts at: 856 856
Size: 3836
BamHI G'GATC_C
Cuts at: 861 861
Size: 3836
BanI G'GyrC_C
Cuts at: 509 852 1266 1514 1549 509
Size: 343 414 248 35 2796
Fragments arranged by size:
2796 414 343 248 35
BanII G_rGCy'C
Cuts at: 850 1880 850
Size: 1030 2806
BbeI G_GCGC'C
Cuts at: 1270 1518 1270
Size: 248 3588
BbsI GAAGACnn'nnnn_
Cuts at: 491 688 491
Size: 197 3639
BciVI GTATCCnnnnn_n'
Cuts at: 1734 2790 3836 1734
Size: 1056 1046 1734
Fragments arranged by size:
1734 1056 1046
BclI T'GATC_A
Cuts at: 1356 1356
Size: 3836
BglI GCCn_nnn'nGGC
Cuts at: 161 161
Size: 3836
BglII A'GATC_T
Cuts at: 1351 3388 1351
Size: 2037 1799
BlpI GC'TnA_GC
Cuts at: 810 948 810
Size: 138 3698
Bme1580I G_kGCm'C
Cuts at: 1461 1554 2678 1461
Size: 93 1124 2619
Fragments arranged by size:
2619 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 205 373 387 1048 1129 1464 3608 205
Size: 168 14 661 81 335 2144 433
Fragments arranged by size:
2144 661 433 335 168 81 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 3836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 658 186
Size: 472 3364
Bpu10I CC'TnA_GC
Cuts at: 583 583
Size: 3836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1891 2605 2903 1012
Size: 879 714 298 1945
Fragments arranged by size:
1945 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3540 3540
Size: 3836
BsaAI yAC'GTr
Cuts at: 801 1189 801
Size: 388 3448
BsaBI GATnn'nnATC
Cuts at: 6 1350 3387 6
Size: 1344 2037 455
Fragments arranged by size:
2037 1344 455
BsaHI Gr'CG_yC
Cuts at: 1267 1515 2217 3713 1267
Size: 248 702 1496 1390
Fragments arranged by size:
1496 1390 702 248
BsaWI w'CCGG_w
Cuts at: 494 611 1025 1546 2224 2635 2782 3056 494
Size: 117 414 521 678 411 147 274 1274
Fragments arranged by size:
1274 678 521 414 411 274 147 117
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 183 619 819 183
Size: 436 200 3200
Fragments arranged by size:
3200 436 200
BseYI C'CCAG_C
Cuts at: 121 1261 2019 2684 3092 121
Size: 1140 758 665 408 865
Fragments arranged by size:
1140 865 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 132
Size: 3836
BsiEI CG_ry'CG
Cuts at: 104 601 1378 1424 2654 104
Size: 497 777 46 1230 1286
Fragments arranged by size:
1286 1230 777 497 46
BsiHKAI G_wGCw'C
Cuts at: 175 850 1628 1818 2678 175
Size: 675 778 190 860 1333
Fragments arranged by size:
1333 860 778 675 190
Bsp1286I G_dGCh'C
Cuts at: 175 850 1461 1554 1628 1818 1880 2678 175
Size: 675 611 93 74 190 62 798 1333
Fragments arranged by size:
1333 798 675 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 494 494
Size: 3836
BspHI T'CATG_A
Cuts at: 128 2268 128
Size: 2140 1696
BspMI ACCTGCnnnn'nnnn_
Cuts at: 886 1100 1402 1783 886
Size: 214 302 381 2939
Fragments arranged by size:
2939 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2128 2182 3666 3831 50
Size: 2078 54 1484 165 55
Fragments arranged by size:
2078 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1748 1748
Size: 3836
BsrFI r'CCGG_y
Cuts at: 160 596 1834 2015 160
Size: 436 1238 181 1981
Fragments arranged by size:
1981 1238 436 181
BssSI C'ACGA_G
Cuts at: 370 2107 2815 370
Size: 1737 708 1391
Fragments arranged by size:
1737 1391 708
BstAPI GCAn_nnn'nTGC
Cuts at: 648 1213 648
Size: 565 3271
BstBI TT'CG_AA
Cuts at: 2197 2197
Size: 3836
BstXI CCAn_nnnn'nTGG
Cuts at: 636 720 636
Size: 84 3752
BstYI r'GATC_y
Cuts at: 1 861 1328 1351 1686 1932 2238 2250 2336
Size: 860 467 23 335 246 306 12 86
Cuts at: 2336 3388 1
Size: 1052 449
Fragments arranged by size:
1052 860 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 277 277
Size: 3836
BtgI C'CryG_G
Cuts at: 245 245
Size: 3836
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1786 1967 2130 1786
Size: 181 163 3492
Fragments arranged by size:
3492 181 163
BtsI GCAGTG_nn'
Cuts at: 629 1079 629
Size: 450 3386
DraI TTT'AAA
Cuts at: 835 835
Size: 3836
DrdI GACnn_nn'nnGTC
Cuts at: 256 1542 2886 256
Size: 1286 1344 1206
Fragments arranged by size:
1344 1286 1206
EaeI y'GGCC_r
Cuts at: 626 710 722 1421 1595 1986 2013 3358 626
Size: 84 12 699 174 391 27 1345 1104
Fragments arranged by size:
1345 1104 699 391 174 84 27 12
EagI C'GGCC_G
Cuts at: 1421 1421
Size: 3836
EarI CTCTTCn'nnn_
Cuts at: 1859 2069 1859
Size: 210 3626
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2044 2786 2932 3646 2044
Size: 742 146 714 2234
Fragments arranged by size:
2234 742 714 146
EcoICRI GAG'CTC
Cuts at: 848 848
Size: 3836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 658 1661 2093 2446 3108 186
Size: 472 1003 432 353 662 914
Fragments arranged by size:
1003 914 662 472 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 517 801 517
Size: 284 3552
EcoO109I rG'GnC_Cy
Cuts at: 621 975 621
Size: 354 3482
EcoRI G'AATT_C
Cuts at: 840 840
Size: 3836
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1855 1855
Size: 3836
FspI TGC'GCA
Cuts at: 1617 1617
Size: 3836
HaeII r_GCGC'y
Cuts at: 1270 1518 2748 3519 1270
Size: 248 1230 771 1587
Fragments arranged by size:
1587 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1678 1708 1710 1740 1678
Size: 30 2 30 3774
Fragments arranged by size:
3774 30 30 2
HincII GTy'rAC
Cuts at: 261 875 261
Size: 614 3222
KasI G'GCGC_C
Cuts at: 1266 1514 1266
Size: 248 3588
KpnI G_GTAC'C
Cuts at: 856 856
Size: 3836
MluI A'CGCG_T
Cuts at: 3269 3269
Size: 3836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 202 581 2595 2779 202
Size: 379 2014 184 1259
Fragments arranged by size:
2014 1259 379 184
MscI TGG'CCA
Cuts at: 628 712 724 1597 628
Size: 84 12 873 2867
Fragments arranged by size:
2867 873 84 12
MslI CAynn'nnrTG
Cuts at: 691 766 799 1825 1952 691
Size: 75 33 1026 127 2575
Fragments arranged by size:
2575 1026 127 75 33
MspA1I CmG'CkG
Cuts at: 116 645 947 1261 1621 2405 2650 116
Size: 529 302 314 360 784 245 1302
Fragments arranged by size:
1302 784 529 360 314 302 245
NaeI GCC'GGC
Cuts at: 162 2017 162
Size: 1855 1981
NarI GG'CG_CC
Cuts at: 1267 1515 1267
Size: 248 3588
NcoI C'CATG_G
Cuts at: 245 245
Size: 3836
NgoMIV G'CCGG_C
Cuts at: 160 2015 160
Size: 1855 1981
NspI r_CATG'y
Cuts at: 889 1389 2992 889
Size: 500 1603 1733
Fragments arranged by size:
1733 1603 500
PciI A'CATG_T
Cuts at: 2988 2988
Size: 3836
PflMI CCAn_nnn'nTGG
Cuts at: 246 246
Size: 3836
PmeI GTTT'AAAC
Cuts at: 835 835
Size: 3836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2282 2314 2282
Size: 32 3804
PpuMI rG'GwC_Cy
Cuts at: 621 621
Size: 3836
PstI C_TGCA'G
Cuts at: 419 581 653 883 1568 419
Size: 162 72 230 685 2687
Fragments arranged by size:
2687 685 230 162 72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3836
PvuII CAG'CTG
Cuts at: 645 1261 1621 645
Size: 616 360 2860
Fragments arranged by size:
2860 616 360
RsrII CG'GwC_CG
Cuts at: 2031 2031
Size: 3836
SacI G_AGCT'C
Cuts at: 850 850
Size: 3836
SalI G'TCGA_C
Cuts at: 873 873
Size: 3836
SapI GCTCTTCn'nnn_
Cuts at: 1859 2069 1859
Size: 210 3626
SbfI CC_TGCA'GG
Cuts at: 883 883
Size: 3836
ScaI AGT'ACT
Cuts at: 3353 3353
Size: 3836
SfcI C'TryA_G
Cuts at: 33 415 577 649 879 1564 2532 2723 33
Size: 382 162 72 230 685 968 191 1146
Fragments arranged by size:
1146 968 685 382 230 191 162 72
SfoI GGC'GCC
Cuts at: 1268 1516 1268
Size: 248 3588
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
SmaI CCC'GGG
Cuts at: 858 858
Size: 3836
SmlI C'TyrA_G
Cuts at: 991 1906 2620 2882 991
Size: 915 714 262 1945
Fragments arranged by size:
1945 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3836
SphI G_CATG'C
Cuts at: 889 1389 889
Size: 500 3336
SspI AAT'ATT
Cuts at: 501 501
Size: 3836
StuI AGG'CCT
Cuts at: 655 655
Size: 3836
StyI C'CwwG_G
Cuts at: 245 970 1324 245
Size: 725 354 2757
Fragments arranged by size:
2757 725 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1419 1552 2011 2220 1419
Size: 133 459 209 3035
Fragments arranged by size:
3035 459 209 133
TatI w'GTAC_w
Cuts at: 3351 3351
Size: 3836
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 721 753 3353 3385 721
Size: 32 2600 32 1172
Fragments arranged by size:
2600 1172 32 32
Tth111I GACn'n_nGTC
Cuts at: 1633 1633
Size: 3836
XbaI T'CTAG_A
Cuts at: 867 867
Size: 3836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 693 822 1331 693
Size: 129 509 3198
Fragments arranged by size:
3198 509 129
XmaI C'CCGG_G
Cuts at: 856 856
Size: 3836
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI XmnI ZraI