Restriction map for
pF1KB5225
(Circular) (Six-base) MAPSORT of: pF1KB5225.seq Check: 4550 from: 1 to: 5216
pF1KB5225 5216 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 959 1132 959
Size: 173 5043
AatII G_ACGT'C
Cuts at: 476 1932 476
Size: 1456 3760
AccI GT'mk_AC
Cuts at: 2254 2254
Size: 5216
Acc65I G'GTAC_C
Cuts at: 1307 2232 1307
Size: 925 4291
AclI AA'CG_TT
Cuts at: 2210 5056 2210
Size: 2846 2370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 721 1087 1249 1670 3041 3473 3826 4488
Size: 21 366 162 421 1371 432 353 662
Cuts at: 4488 700
Size: 1428
Fragments arranged by size:
1428 1371 662 432 421 366 353 162
21
AflIII A'CryG_T
Cuts at: 1535 2568 4368 4649 1535
Size: 1033 1800 281 2102
Fragments arranged by size:
2102 1800 1033 281
AleI CACnn'nnGTG
Cuts at: 1615 1615
Size: 5216
AlwNI CAG_nnn'CTG
Cuts at: 222 1714 2521 3959 222
Size: 1492 807 1438 1479
Fragments arranged by size:
1492 1479 1438 807
ApaI G_GGCC'C
Cuts at: 1245 1245
Size: 5216
ApaLI G'TGCA_C
Cuts at: 4054 4054
Size: 5216
ApoI r'AATT_y
Cuts at: 600 2220 4569 4825 600
Size: 1620 2349 256 991
Fragments arranged by size:
2349 1620 991 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5216
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5216
AvaI C'yCGr_G
Cuts at: 633 1285 2236 633
Size: 652 951 3613
Fragments arranged by size:
3613 951 652
AvrII C'CTAG_G
Cuts at: 621 621
Size: 5216
BamHI G'GATC_C
Cuts at: 2241 2241
Size: 5216
BanI G'GyrC_C
Cuts at: 1144 1307 1719 1812 2003 2232 2646 2894 2929
Size: 163 412 93 191 229 414 248 35
Cuts at: 2929 1144
Size: 3431
Fragments arranged by size:
3431 414 412 248 229 191 163 93
35
BanII G_rGCy'C
Cuts at: 838 1245 1956 2130 2230 3260 838
Size: 407 711 174 100 1030 2794
Fragments arranged by size:
2794 1030 711 407 174 100
BbeI G_GCGC'C
Cuts at: 1816 2007 2650 2898 1816
Size: 191 643 248 4134
Fragments arranged by size:
4134 643 248 191
BbsI GAAGACnn'nnnn_
Cuts at: 1448 1448
Size: 5216
BbvCI CC'TCA_GC
Cuts at: 862 1294 1991 862
Size: 432 697 4087
Fragments arranged by size:
4087 697 432
BciVI GTATCCnnnnn_n'
Cuts at: 3114 4170 5216 3114
Size: 1056 1046 3114
Fragments arranged by size:
3114 1056 1046
BclI T'GATC_A
Cuts at: 2736 2736
Size: 5216
BglI GCCn_nnn'nGGC
Cuts at: 238 626 1821 2001 2134 238
Size: 388 1195 180 133 3320
Fragments arranged by size:
3320 1195 388 180 133
BglII A'GATC_T
Cuts at: 2731 4768 2731
Size: 2037 3179
BlpI GC'TnA_GC
Cuts at: 857 2328 857
Size: 1471 3745
Bme1580I G_kGCm'C
Cuts at: 1149 1245 1622 2841 2934 4058 1149
Size: 96 377 1219 93 1124 2307
Fragments arranged by size:
2307 1219 1124 377 96 93
BmrI ACTGGGnnnn_n'
Cuts at: 1771 2062 2428 2509 2844 4988 1771
Size: 291 366 81 335 2144 1999
Fragments arranged by size:
2144 1999 366 335 291 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1137 3583 1137
Size: 2446 2770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 484 526 820 1129 1538 1549 1640 1646
Size: 240 42 294 309 409 11 91 6
Cuts at: 1646 1853 244
Size: 207 3607
Fragments arranged by size:
3607 409 309 294 240 207 91 42
11 6
Bpu10I CC'TnA_GC
Cuts at: 862 1294 1991 862
Size: 432 697 4087
Fragments arranged by size:
4087 697 432
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 1060 1135 2392 3271 3985 4283 556
Size: 504 75 1257 879 714 298 1489
Fragments arranged by size:
1489 1257 879 714 504 298 75
BsaI GGTCTCn'nnnn_
Cuts at: 169 1039 1851 2197 4920 169
Size: 870 812 346 2723 465
Fragments arranged by size:
2723 870 812 465 346
BsaAI yAC'GTr
Cuts at: 2569 2569
Size: 5216
BsaBI GATnn'nnATC
Cuts at: 6 818 2730 4767 6
Size: 812 1912 2037 455
Fragments arranged by size:
2037 1912 812 455
BsaHI Gr'CG_yC
Cuts at: 473 1475 1813 1929 2004 2647 2895 3597 5093
Size: 1002 338 116 75 643 248 702 1496
Cuts at: 5093 473
Size: 596
Fragments arranged by size:
1496 1002 702 643 596 338 248 116
75
BsaWI w'CCGG_w
Cuts at: 2405 2926 3604 4015 4162 4436 2405
Size: 521 678 411 147 274 3185
Fragments arranged by size:
3185 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1626 1656 1626
Size: 30 5186
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 517 520 700 703 706 709 1126 1354 1851
Size: 3 180 3 3 3 417 228 497
Cuts at: 1851 2138 517
Size: 287 3595
Fragments arranged by size:
3595 497 417 287 228 180 3 3
3 3
BseYI C'CCAG_C
Cuts at: 960 1051 1366 1433 1626 1674 2032 2062 2160
Size: 91 315 67 193 48 358 30 98
Cuts at: 2160 2641 3399 4064 4472 960
Size: 481 758 665 408 1704
Fragments arranged by size:
1704 758 665 481 408 358 315 193
98 91 67 48 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 1290 2072 109
Size: 1181 782 3253
Fragments arranged by size:
3253 1181 782
BsiEI CG_ry'CG
Cuts at: 104 2758 2804 4034 104
Size: 2654 46 1230 1286
Fragments arranged by size:
2654 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 838 2230 3008 3198 4058 838
Size: 1392 778 190 860 1996
Fragments arranged by size:
1996 1392 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 667 799 1078 667
Size: 132 279 4805
Fragments arranged by size:
4805 279 132
Bsp1286I G_dGCh'C
Cuts at: 838 1149 1245 1622 1956 2130 2230 2841 2934
Size: 311 96 377 334 174 100 611 93
Cuts at: 2934 3008 3198 3260 4058 838
Size: 74 190 62 798 1996
Fragments arranged by size:
1996 798 611 377 334 311 190 174
100 96 93 74 62
BspHI T'CATG_A
Cuts at: 998 3648 998
Size: 2650 2566
BspMI ACCTGCnnnn'nnnn_
Cuts at: 198 209 815 959 1132 2266 2480 2782 3163
Size: 11 606 144 173 1134 214 302 381
Cuts at: 3163 198
Size: 2251
Fragments arranged by size:
2251 1134 606 381 302 214 173 144
11
BsrBI CCG'CTC
Cuts at: 50 290 1859 3508 3562 5046 5211 50
Size: 240 1569 1649 54 1484 165 55
Fragments arranged by size:
1649 1569 1484 240 165 55 54
BsrDI GCAATG_nn'
Cuts at: 724 3128 724
Size: 2404 2812
BsrFI r'CCGG_y
Cuts at: 228 2012 3214 3395 228
Size: 1784 1202 181 2049
Fragments arranged by size:
2049 1784 1202 181
BssSI C'ACGA_G
Cuts at: 550 3487 4195 550
Size: 2937 708 1571
Fragments arranged by size:
2937 1571 708
BstAPI GCAn_nnn'nTGC
Cuts at: 132 2593 132
Size: 2461 2755
BstBI TT'CG_AA
Cuts at: 3577 3577
Size: 5216
BstEII G'GTnAC_C
Cuts at: 1746 1746
Size: 5216
BstXI CCAn_nnnn'nTGG
Cuts at: 348 669 1386 1467 348
Size: 321 717 81 4097
Fragments arranged by size:
4097 717 321 81
BstYI r'GATC_y
Cuts at: 1 2241 2708 2731 3066 3312 3618 3630 3716
Size: 2240 467 23 335 246 306 12 86
Cuts at: 3716 4768 1
Size: 1052 449
Fragments arranged by size:
2240 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1303 2132 1303
Size: 829 4387
BtgI C'CryG_G
Cuts at: 656 873 656
Size: 217 4999
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 805 1359 3166 3347 3510 805
Size: 554 1807 181 163 2511
Fragments arranged by size:
2511 1807 554 181 163
BtsI GCAGTG_nn'
Cuts at: 1610 2459 1610
Size: 849 4367
DraI TTT'AAA
Cuts at: 2215 2215
Size: 5216
DraIII CAC_nnn'GTG
Cuts at: 1137 1137
Size: 5216
DrdI GACnn_nn'nnGTC
Cuts at: 2922 4266 2922
Size: 1344 3872
EaeI y'GGCC_r
Cuts at: 230 443 659 876 1334 1405 2801 2975 3366
Size: 213 216 217 458 71 1396 174 391
Cuts at: 3366 3393 4738 230
Size: 27 1345 708
Fragments arranged by size:
1396 1345 708 458 391 217 216 213
174 71 27
EagI C'GGCC_G
Cuts at: 2801 2801
Size: 5216
EarI CTCTTCn'nnn_
Cuts at: 109 697 1022 1042 1516 1552 3239 3449 109
Size: 588 325 20 474 36 1687 210 1876
Fragments arranged by size:
1876 1687 588 474 325 210 36 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3424 4166 4312 5026 3424
Size: 742 146 714 3614
Fragments arranged by size:
3614 742 714 146
EcoICRI GAG'CTC
Cuts at: 836 2228 836
Size: 1392 3824
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 484 526 700 721 820 1087 1129 1249
Size: 240 42 174 21 99 267 42 120
Cuts at: 1249 1538 1549 1640 1646 1670 1853 3041 3473
Size: 289 11 91 6 24 183 1188 432
Cuts at: 3473 3826 4488 244
Size: 353 662 972
Fragments arranged by size:
1188 972 662 432 353 289 267 240
183 174 120 99 91 42 42 24
21 11 6
EcoNI CCTnn'n_nnAGG
Cuts at: 193 205 1261 193
Size: 12 1056 4148
Fragments arranged by size:
4148 1056 12
EcoO109I rG'GnC_Cy
Cuts at: 198 1055 1241 1254 1390 1907 2148 2355 198
Size: 857 186 13 136 517 241 207 3059
Fragments arranged by size:
3059 857 517 241 207 186 136 13
EcoRI G'AATT_C
Cuts at: 600 2220 600
Size: 1620 3596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3235 3235
Size: 5216
FspI TGC'GCA
Cuts at: 2997 2997
Size: 5216
HaeII r_GCGC'y
Cuts at: 1489 1809 1816 2007 2650 2898 4128 4899 1489
Size: 320 7 191 643 248 1230 771 1806
Fragments arranged by size:
1806 1230 771 643 320 248 191 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1432 1464 3058 3088 3090 3120 1432
Size: 32 1594 30 2 30 3528
Fragments arranged by size:
3528 1594 32 30 30 2
HincII GTy'rAC
Cuts at: 2255 2255
Size: 5216
HindIII A'AGCT_T
Cuts at: 319 319
Size: 5216
KasI G'GCGC_C
Cuts at: 1812 2003 2646 2894 1812
Size: 191 643 248 4134
Fragments arranged by size:
4134 643 248 191
KpnI G_GTAC'C
Cuts at: 1311 2236 1311
Size: 925 4291
MluI A'CGCG_T
Cuts at: 4649 4649
Size: 5216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 581 1148 3975 4159 581
Size: 567 2827 184 1638
Fragments arranged by size:
2827 1638 567 184
MscI TGG'CCA
Cuts at: 661 1407 2977 661
Size: 746 1570 2900
Fragments arranged by size:
2900 1570 746
MslI CAynn'nnrTG
Cuts at: 346 1465 1615 3205 3332 346
Size: 1119 150 1590 127 2230
Fragments arranged by size:
2230 1590 1119 150 127
MspA1I CmG'CkG
Cuts at: 123 141 181 219 429 1437 1555 1771 2327
Size: 18 40 38 210 1008 118 216 556
Cuts at: 2327 2641 3001 3785 4030 123
Size: 314 360 784 245 1309
Fragments arranged by size:
1309 1008 784 556 360 314 245 216
210 118 40 38 18
NaeI GCC'GGC
Cuts at: 230 2014 3397 230
Size: 1784 1383 2049
Fragments arranged by size:
2049 1784 1383
NarI GG'CG_CC
Cuts at: 1813 2004 2647 2895 1813
Size: 191 643 248 4134
Fragments arranged by size:
4134 643 248 191
NcoI C'CATG_G
Cuts at: 656 656
Size: 5216
NgoMIV G'CCGG_C
Cuts at: 228 2012 3395 228
Size: 1784 1383 2049
Fragments arranged by size:
2049 1784 1383
NspI r_CATG'y
Cuts at: 135 2269 2769 4372 135
Size: 2134 500 1603 979
Fragments arranged by size:
2134 1603 979 500
PasI CC'CwG_GG
Cuts at: 920 1903 920
Size: 983 4233
PciI A'CATG_T
Cuts at: 4368 4368
Size: 5216
PflMI CCAn_nnn'nTGG
Cuts at: 1107 1470 1107
Size: 363 4853
PfoI T'CCnGG_A
Cuts at: 797 1565 797
Size: 768 4448
PmeI GTTT'AAAC
Cuts at: 2215 2215
Size: 5216
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3662 3694 3662
Size: 32 5184
PpuMI rG'GwC_Cy
Cuts at: 198 1390 1907 2148 198
Size: 1192 517 241 3266
Fragments arranged by size:
3266 1192 517 241
PspOMI G'GGCC_C
Cuts at: 1241 1241
Size: 5216
PstI C_TGCA'G
Cuts at: 209 269 281 482 1253 2263 2948 209
Size: 60 12 201 771 1010 685 2477
Fragments arranged by size:
2477 1010 771 685 201 60 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5216
PvuII CAG'CTG
Cuts at: 123 141 429 1437 1555 1771 2641 3001 123
Size: 18 288 1008 118 216 870 360 2338
Fragments arranged by size:
2338 1008 870 360 288 216 118 18
RsrII CG'GwC_CG
Cuts at: 3411 3411
Size: 5216
SacI G_AGCT'C
Cuts at: 838 2230 838
Size: 1392 3824
SalI G'TCGA_C
Cuts at: 2253 2253
Size: 5216
SanDI GG'GwC_CC
Cuts at: 1390 1907 2148 1390
Size: 517 241 4458
Fragments arranged by size:
4458 517 241
SapI GCTCTTCn'nnn_
Cuts at: 3239 3449 3239
Size: 210 5006
SbfI CC_TGCA'GG
Cuts at: 2263 2263
Size: 5216
ScaI AGT'ACT
Cuts at: 733 4733 733
Size: 4000 1216
SfcI C'TryA_G
Cuts at: 33 205 265 277 478 1249 2259 2944 3912
Size: 172 60 12 201 771 1010 685 968
Cuts at: 3912 4103 33
Size: 191 1146
Fragments arranged by size:
1146 1010 968 771 685 201 191 172
60 12
SfoI GGC'GCC
Cuts at: 1814 2005 2648 2896 1814
Size: 191 643 248 4134
Fragments arranged by size:
4134 643 248 191
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5216
SmaI CCC'GGG
Cuts at: 635 2238 635
Size: 1603 3613
SmlI C'TyrA_G
Cuts at: 571 1075 1150 2371 3286 4000 4262 571
Size: 504 75 1221 915 714 262 1525
Fragments arranged by size:
1525 1221 915 714 504 262 75
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5216
SphI G_CATG'C
Cuts at: 135 2269 2769 135
Size: 2134 500 2582
Fragments arranged by size:
2582 2134 500
StuI AGG'CCT
Cuts at: 271 397 620 991 1544 271
Size: 126 223 371 553 3943
Fragments arranged by size:
3943 553 371 223 126
StyI C'CwwG_G
Cuts at: 398 621 656 761 1679 1697 1742 2350 2704
Size: 223 35 105 918 18 45 608 354
Cuts at: 2704 398
Size: 2910
Fragments arranged by size:
2910 918 608 354 223 105 45 35
18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 181 925 1380 2799 2932 3391 3600 181
Size: 744 455 1419 133 459 209 1797
Fragments arranged by size:
1797 1419 744 459 455 209 133
TatI w'GTAC_w
Cuts at: 731 4731 731
Size: 4000 1216
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1626 1658 1740 1772 4733 4765 1626
Size: 32 82 32 2961 32 2077
Fragments arranged by size:
2961 2077 82 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1573 1710 3013 1573
Size: 137 1303 3776
Fragments arranged by size:
3776 1303 137
XbaI T'CTAG_A
Cuts at: 2247 2247
Size: 5216
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1467 2711 1467
Size: 1244 3972
XmaI C'CCGG_G
Cuts at: 633 2236 633
Size: 1603 3613
ZraI GAC'GTC
Cuts at: 474 1930 474
Size: 1456 3760
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AlfI AloI AscI BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BspEI BsrGI
BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI