Restriction map for
pF1KB5227
(Circular) (Six-base) MAPSORT of: pF1KB5227.seq Check: 6216 from: 1 to: 4595
pF1KB5227 4595 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 873 873
Size: 4595
AccI GT'mk_AC
Cuts at: 241 1138 1633 241
Size: 897 495 3203
Fragments arranged by size:
3203 897 495
Acc65I G'GTAC_C
Cuts at: 1611 1611
Size: 4595
AclI AA'CG_TT
Cuts at: 4435 4435
Size: 4595
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 2420 2852 3205 3867 154
Size: 2266 432 353 662 882
Fragments arranged by size:
2266 882 662 432 353
AflIII A'CryG_T
Cuts at: 1947 3747 4028 1947
Size: 1800 281 2514
Fragments arranged by size:
2514 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 250 880 1900 3338 250
Size: 630 1020 1438 1507
Fragments arranged by size:
1507 1438 1020 630
ApaLI G'TGCA_C
Cuts at: 3433 3433
Size: 4595
ApoI r'AATT_y
Cuts at: 806 1599 3948 4204 806
Size: 793 2349 256 1197
Fragments arranged by size:
2349 1197 793 256
AseI AT'TA_AT
Cuts at: 20 595 20
Size: 575 4020
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4595
AvaI C'yCGr_G
Cuts at: 1615 1615
Size: 4595
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1282 1315 1282
Size: 33 4562
BamHI G'GATC_C
Cuts at: 1620 1620
Size: 4595
BanI G'GyrC_C
Cuts at: 1512 1611 2025 2273 2308 1512
Size: 99 414 248 35 3799
Fragments arranged by size:
3799 414 248 99 35
BanII G_rGCy'C
Cuts at: 1609 2639 1609
Size: 1030 3565
BbeI G_GCGC'C
Cuts at: 2029 2277 2029
Size: 248 4347
BbvCI CC'TCA_GC
Cuts at: 509 509
Size: 4595
BciVI GTATCCnnnnn_n'
Cuts at: 1459 2493 3549 4595 1459
Size: 1034 1056 1046 1459
Fragments arranged by size:
1459 1056 1046 1034
BclI T'GATC_A
Cuts at: 2115 2115
Size: 4595
BglII A'GATC_T
Cuts at: 2110 4147 2110
Size: 2037 2558
BlpI GC'TnA_GC
Cuts at: 255 1707 255
Size: 1452 3143
Bme1580I G_kGCm'C
Cuts at: 1287 1517 2220 2313 3437 1287
Size: 230 703 93 1124 2445
Fragments arranged by size:
2445 1124 703 230 93
BmgBI CAC'GTC
Cuts at: 799 799
Size: 4595
BmrI ACTGGGnnnn_n'
Cuts at: 678 917 1807 1888 2223 4367 678
Size: 239 890 81 335 2144 906
Fragments arranged by size:
2144 906 890 335 239 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2962 2962
Size: 4595
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 884 563
Size: 321 4274
Bpu10I CC'TnA_GC
Cuts at: 509 1031 509
Size: 522 4073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 806 942 1771 2650 3364 3662 806
Size: 136 829 879 714 298 1739
Fragments arranged by size:
1739 879 829 714 298 136
BsaI GGTCTCn'nnnn_
Cuts at: 1404 1551 4299 1404
Size: 147 2748 1700
Fragments arranged by size:
2748 1700 147
BsaAI yAC'GTr
Cuts at: 1948 1948
Size: 4595
BsaBI GATnn'nnATC
Cuts at: 6 2109 4146 6
Size: 2103 2037 455
Fragments arranged by size:
2103 2037 455
BsaHI Gr'CG_yC
Cuts at: 2026 2274 2976 4472 2026
Size: 248 702 1496 2149
Fragments arranged by size:
2149 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1784 2305 2983 3394 3541 3815 1784
Size: 521 678 411 147 274 2564
Fragments arranged by size:
2564 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 602 1526 602
Size: 924 3671
BseYI C'CCAG_C
Cuts at: 2020 2778 3443 3851 2020
Size: 758 665 408 2764
Fragments arranged by size:
2764 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 851 851
Size: 4595
BsiEI CG_ry'CG
Cuts at: 104 2137 2183 3413 104
Size: 2033 46 1230 1286
Fragments arranged by size:
2033 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 567 1609 2387 2577 3437 567
Size: 1042 778 190 860 1725
Fragments arranged by size:
1725 1042 860 778 190
BsmI GAATG_Cn'
Cuts at: 578 632 773 578
Size: 54 141 4400
Fragments arranged by size:
4400 141 54
Bsp1286I G_dGCh'C
Cuts at: 567 1287 1517 1609 2220 2313 2387 2577 2639
Size: 720 230 92 611 93 74 190 62
Cuts at: 2639 3437 567
Size: 798 1725
Fragments arranged by size:
1725 798 720 611 230 190 93 92
74 62
BspHI T'CATG_A
Cuts at: 3027 3027
Size: 4595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 264 873 894 1645 1859 2161 2542 264
Size: 609 21 751 214 302 381 2317
Fragments arranged by size:
2317 751 609 381 302 214 21
BsrBI CCG'CTC
Cuts at: 50 2887 2941 4425 4590 50
Size: 2837 54 1484 165 55
Fragments arranged by size:
2837 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 264 2507 264
Size: 2243 2352
BsrFI r'CCGG_y
Cuts at: 2593 2774 2593
Size: 181 4414
BssSI C'ACGA_G
Cuts at: 2866 3574 2866
Size: 708 3887
BstAPI GCAn_nnn'nTGC
Cuts at: 1972 1972
Size: 4595
BstBI TT'CG_AA
Cuts at: 2956 2956
Size: 4595
BstYI r'GATC_y
Cuts at: 1 179 1620 2087 2110 2445 2691 2997 3009
Size: 178 1441 467 23 335 246 306 12
Cuts at: 3009 3095 4147 1
Size: 86 1052 449
Fragments arranged by size:
1441 1052 467 449 335 306 246 178
86 23 12
BstZ17I GTA'TAC
Cuts at: 242 242
Size: 4595
BtgI C'CryG_G
Cuts at: 107 139 107
Size: 32 4563
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2545 2726 2889 2545
Size: 181 163 4251
Fragments arranged by size:
4251 181 163
BtsI GCAGTG_nn'
Cuts at: 1838 1838
Size: 4595
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 641 676 641
Size: 35 4560
DraI TTT'AAA
Cuts at: 213 1594 213
Size: 1381 3214
DrdI GACnn_nn'nnGTC
Cuts at: 2301 3645 2301
Size: 1344 3251
EaeI y'GGCC_r
Cuts at: 1318 1581 2180 2354 2745 2772 4117 1318
Size: 263 599 174 391 27 1345 1796
Fragments arranged by size:
1796 1345 599 391 263 174 27
EagI C'GGCC_G
Cuts at: 2180 2180
Size: 4595
EarI CTCTTCn'nnn_
Cuts at: 1268 2618 2828 1268
Size: 1350 210 3035
Fragments arranged by size:
3035 1350 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2803 3545 3691 4405 2803
Size: 742 146 714 2993
Fragments arranged by size:
2993 742 714 146
EcoICRI GAG'CTC
Cuts at: 1607 1607
Size: 4595
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 563 884 2420 2852 3205 3867 154
Size: 409 321 1536 432 353 662 882
Fragments arranged by size:
1536 882 662 432 409 353 321
EcoNI CCTnn'n_nnAGG
Cuts at: 512 740 1319 512
Size: 228 579 3788
Fragments arranged by size:
3788 579 228
EcoO109I rG'GnC_Cy
Cuts at: 247 1048 1329 1734 247
Size: 801 281 405 3108
Fragments arranged by size:
3108 801 405 281
EcoRI G'AATT_C
Cuts at: 806 1599 806
Size: 793 3802
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 983 2614 983
Size: 1631 2964
FspI TGC'GCA
Cuts at: 2376 2376
Size: 4595
HaeII r_GCGC'y
Cuts at: 844 2029 2277 3507 4278 844
Size: 1185 248 1230 771 1161
Fragments arranged by size:
1230 1185 1161 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 451 483 1249 1281 2437 2467 2469 2499 451
Size: 32 766 32 1156 30 2 30 2547
Fragments arranged by size:
2547 1156 766 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1634 1634
Size: 4595
KasI G'GCGC_C
Cuts at: 2025 2273 2025
Size: 248 4347
KpnI G_GTAC'C
Cuts at: 1615 1615
Size: 4595
MfeI C'AATT_G
Cuts at: 1358 1358
Size: 4595
MluI A'CGCG_T
Cuts at: 4028 4028
Size: 4595
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 317 1332 3354 3538 317
Size: 1015 2022 184 1374
Fragments arranged by size:
2022 1374 1015 184
MscI TGG'CCA
Cuts at: 2356 2356
Size: 4595
MslI CAynn'nnrTG
Cuts at: 709 1253 2584 2711 709
Size: 544 1331 127 2593
Fragments arranged by size:
2593 1331 544 127
MspA1I CmG'CkG
Cuts at: 132 1039 1186 1706 2020 2380 3164 3409 132
Size: 907 147 520 314 360 784 245 1318
Fragments arranged by size:
1318 907 784 520 360 314 245 147
NaeI GCC'GGC
Cuts at: 2776 2776
Size: 4595
NarI GG'CG_CC
Cuts at: 2026 2274 2026
Size: 248 4347
NcoI C'CATG_G
Cuts at: 107 107
Size: 4595
NgoMIV G'CCGG_C
Cuts at: 2774 2774
Size: 4595
NspI r_CATG'y
Cuts at: 273 651 1461 1648 2148 3751 273
Size: 378 810 187 500 1603 1117
Fragments arranged by size:
1603 1117 810 500 378 187
PciI A'CATG_T
Cuts at: 3747 3747
Size: 4595
PflMI CCAn_nnn'nTGG
Cuts at: 721 802 1351 1429 721
Size: 81 549 78 3887
Fragments arranged by size:
3887 549 81 78
PfoI T'CCnGG_A
Cuts at: 801 801
Size: 4595
PmeI GTTT'AAAC
Cuts at: 1594 1594
Size: 4595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3041 3073 3041
Size: 32 4563
PpuMI rG'GwC_Cy
Cuts at: 247 1048 247
Size: 801 3794
PshAI GACnn'nnGTC
Cuts at: 946 946
Size: 4595
PstI C_TGCA'G
Cuts at: 474 1176 1642 2327 474
Size: 702 466 685 2742
Fragments arranged by size:
2742 702 685 466
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4595
PvuII CAG'CTG
Cuts at: 132 1039 2020 2380 132
Size: 907 981 360 2347
Fragments arranged by size:
2347 981 907 360
RsrII CG'GwC_CG
Cuts at: 2790 2790
Size: 4595
SacI G_AGCT'C
Cuts at: 1609 1609
Size: 4595
SalI G'TCGA_C
Cuts at: 1632 1632
Size: 4595
SapI GCTCTTCn'nnn_
Cuts at: 2618 2828 2618
Size: 210 4385
SbfI CC_TGCA'GG
Cuts at: 1642 1642
Size: 4595
ScaI AGT'ACT
Cuts at: 1170 4112 1170
Size: 2942 1653
SexAI A'CCwGG_T
Cuts at: 774 774
Size: 4595
SfcI C'TryA_G
Cuts at: 33 470 1097 1139 1172 1638 2323 3291 3482
Size: 437 627 42 33 466 685 968 191
Cuts at: 3482 33
Size: 1146
Fragments arranged by size:
1146 968 685 627 466 437 191 42
33
SfoI GGC'GCC
Cuts at: 2027 2275 2027
Size: 248 4347
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4595
SmaI CCC'GGG
Cuts at: 1617 1617
Size: 4595
SmlI C'TyrA_G
Cuts at: 821 957 1750 2665 3379 3641 821
Size: 136 793 915 714 262 1775
Fragments arranged by size:
1775 915 793 714 262 136
SpeI A'CTAG_T
Cuts at: 67 1153 1225 67
Size: 1086 72 3437
Fragments arranged by size:
3437 1086 72
SphI G_CATG'C
Cuts at: 273 651 1461 1648 2148 273
Size: 378 810 187 500 2720
Fragments arranged by size:
2720 810 500 378 187
StyI C'CwwG_G
Cuts at: 107 1044 1729 2083 107
Size: 937 685 354 2619
Fragments arranged by size:
2619 937 685 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 697 709 2178 2311 2770 2979 697
Size: 12 1469 133 459 209 2313
Fragments arranged by size:
2313 1469 459 209 133 12
TatI w'GTAC_w
Cuts at: 961 1156 1168 4110 961
Size: 195 12 2942 1446
Fragments arranged by size:
2942 1446 195 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4112 4144 4112
Size: 32 4563
Tth111I GACn'n_nGTC
Cuts at: 2392 2392
Size: 4595
XbaI T'CTAG_A
Cuts at: 1626 1626
Size: 4595
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1051 2090 1051
Size: 1039 3556
XmaI C'CCGG_G
Cuts at: 1615 1615
Size: 4595
XmnI GAAnn'nnTTC
Cuts at: 359 359
Size: 4595
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BcgI BfrBI BglI BmtI
BsaXI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI
Bsu36I ClaI DraIII EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI