FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 Query: pF1KB5229, 293 aa 1>>>pF1KB5229 293 - 293 aa - 293 aa Library: /omim/omim.rfq.tfa 60827320 residues in 85289 sequences Statistics: Expectation_n fit: rho(ln(x))= 4.4719+/-0.000399; mu= 19.6665+/- 0.025 mean_var=59.5532+/-12.406, 0's: 0 Z-trim(111.1): 35 B-trim: 0 in 0/51 Lambda= 0.166196 statistics sampled from 19561 (19593) to 19561 sequences Algorithm: FASTA (3.7 Nov 2010) [optimized] Parameters: BL50 matrix (15:-5), open/ext: -10/-2 ktup: 2, E-join: 1 (0.596), E-opt: 0.2 (0.23), width: 16 Scan time: 6.560 The best scores are: opt bits E(85289) NP_006736 (OMIM: 607545,616834) methylsterol monoo ( 293) 2096 511.1 1e-144 XP_005263233 (OMIM: 607545,616834) PREDICTED: meth ( 293) 2096 511.1 1e-144 NP_001017369 (OMIM: 607545,616834) methylsterol mo ( 162) 1166 287.9 8.8e-78 NP_003947 (OMIM: 604551) cholesterol 25-hydroxylas ( 272) 381 99.9 5.9e-21 NP_008849 (OMIM: 602286,607330) lathosterol oxidas ( 299) 279 75.4 1.5e-13 NP_001020127 (OMIM: 602286,607330) lathosterol oxi ( 299) 279 75.4 1.5e-13 XP_006715794 (OMIM: 613738) PREDICTED: alkylglycer ( 423) 150 44.6 0.00039 XP_016867693 (OMIM: 613738) PREDICTED: alkylglycer ( 424) 150 44.6 0.00039 XP_011513704 (OMIM: 613738) PREDICTED: alkylglycer ( 437) 150 44.7 0.0004 NP_001004320 (OMIM: 613738) alkylglycerol monooxyg ( 445) 150 44.7 0.0004 XP_006715793 (OMIM: 613738) PREDICTED: alkylglycer ( 456) 150 44.7 0.00041 >>NP_006736 (OMIM: 607545,616834) methylsterol monooxyge (293 aa) initn: 2096 init1: 2096 opt: 2096 Z-score: 2719.1 bits: 511.1 E(85289): 1e-144 Smith-Waterman score: 2096; 100.0% identity (100.0% similar) in 293 aa overlap (1-293:1-293) 10 20 30 40 50 60 pF1KB5 MATNESVSIFSSASLAVEYVDSLLPENPLQEPFKNAWNYMLNNYTKFQIATWGSLIVHEA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_006 MATNESVSIFSSASLAVEYVDSLLPENPLQEPFKNAWNYMLNNYTKFQIATWGSLIVHEA 10 20 30 40 50 60 70 80 90 100 110 120 pF1KB5 LYFLFCLPGFLFQFIPYMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_006 LYFLFCLPGFLFQFIPYMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFT 70 80 90 100 110 120 130 140 150 160 170 180 pF1KB5 EYFNIPYDWERMPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_006 EYFNIPYDWERMPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPF 130 140 150 160 170 180 190 200 210 220 230 240 pF1KB5 GMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_006 GMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNL 190 200 210 220 230 240 250 260 270 280 290 pF1KB5 IPFYAGSRHHDFHHMNFIGNYASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE ::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_006 IPFYAGSRHHDFHHMNFIGNYASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE 250 260 270 280 290 >>XP_005263233 (OMIM: 607545,616834) PREDICTED: methylst (293 aa) initn: 2096 init1: 2096 opt: 2096 Z-score: 2719.1 bits: 511.1 E(85289): 1e-144 Smith-Waterman score: 2096; 100.0% identity (100.0% similar) in 293 aa overlap (1-293:1-293) 10 20 30 40 50 60 pF1KB5 MATNESVSIFSSASLAVEYVDSLLPENPLQEPFKNAWNYMLNNYTKFQIATWGSLIVHEA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_005 MATNESVSIFSSASLAVEYVDSLLPENPLQEPFKNAWNYMLNNYTKFQIATWGSLIVHEA 10 20 30 40 50 60 70 80 90 100 110 120 pF1KB5 LYFLFCLPGFLFQFIPYMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_005 LYFLFCLPGFLFQFIPYMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFT 70 80 90 100 110 120 130 140 150 160 170 180 pF1KB5 EYFNIPYDWERMPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_005 EYFNIPYDWERMPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPF 130 140 150 160 170 180 190 200 210 220 230 240 pF1KB5 GMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_005 GMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNL 190 200 210 220 230 240 250 260 270 280 290 pF1KB5 IPFYAGSRHHDFHHMNFIGNYASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE ::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_005 IPFYAGSRHHDFHHMNFIGNYASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE 250 260 270 280 290 >>NP_001017369 (OMIM: 607545,616834) methylsterol monoox (162 aa) initn: 1166 init1: 1166 opt: 1166 Z-score: 1517.4 bits: 287.9 E(85289): 8.8e-78 Smith-Waterman score: 1166; 100.0% identity (100.0% similar) in 162 aa overlap (132-293:1-162) 110 120 130 140 150 160 pF1KB5 LFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWYFLLARCFGCAVIEDTWHYFLHRLLH :::::::::::::::::::::::::::::: NP_001 MPRWYFLLARCFGCAVIEDTWHYFLHRLLH 10 20 30 170 180 190 200 210 220 pF1KB5 HKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 HKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRL 40 50 60 70 80 90 230 240 250 260 270 280 pF1KB5 LETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFIGNYASTFTWWDRIFGTDSQYNAY :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 LETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFIGNYASTFTWWDRIFGTDSQYNAY 100 110 120 130 140 150 290 pF1KB5 NEKRKKFEKKTE :::::::::::: NP_001 NEKRKKFEKKTE 160 >>NP_003947 (OMIM: 604551) cholesterol 25-hydroxylase [H (272 aa) initn: 273 init1: 147 opt: 381 Z-score: 497.2 bits: 99.9 E(85289): 5.9e-21 Smith-Waterman score: 381; 28.0% identity (59.3% similar) in 246 aa overlap (37-274:24-263) 10 20 30 40 50 60 pF1KB5 VSIFSSASLAVEYVDSLLPENPLQEPFKNAWNYMLNNYTKFQIATWGSLIVHEALYFLFC :.. : .. . . . .: . : :: NP_003 MSCHNCSDPQVLCSSGQLFLQPLWDH-LRSWEALLQSPFFPVIFSITTYVGFC 10 20 30 40 50 70 80 90 100 110 120 pF1KB5 LP----GFLFQFIPYMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEY :: .: ...: ...:::. : . .. :. :..: . .:. . . NP_003 LPFVVLDILCSWVPALRRYKIHPDFSPSAQQLLPCLGQTLYQHVMFVFPVTLLHWARSPA 60 70 80 90 100 110 130 140 150 160 170 180 pF1KB5 FNIPYDWERMPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKR--IYKYIHKVHHEFQAPF . .:.. :. .:: . . : .. : ..:. .::::: .:. .:::::. .. : NP_003 L-LPHE---APELLLLLHHILFCLLLFDM-EFFVWHLLHHKVPWLYRTFHKVHHQNSSSF 120 130 140 150 160 190 200 210 220 230 240 pF1KB5 GMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNL .. ..: : . :: .....:: : . .. .. . ... ::::..: . : NP_003 ALATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRL 170 180 190 200 210 220 250 260 270 280 290 pF1KB5 IPF--YAGSRHHDFHHMNFIGNYASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE .:: :.: :::.:: .: :.: :: ::.:.:: NP_003 VPFGWYGGVVHHDLHHSHFNCNFAPYFTHWDKILGTLRTASVPAR 230 240 250 260 270 >>NP_008849 (OMIM: 602286,607330) lathosterol oxidase [H (299 aa) initn: 268 init1: 152 opt: 279 Z-score: 364.5 bits: 75.4 E(85289): 1.5e-13 Smith-Waterman score: 279; 29.5% identity (55.1% similar) in 234 aa overlap (61-284:45-263) 40 50 60 70 80 pF1KB5 EPFKNAWNYMLNNYTKFQIATWGSLIVHEALYFLFCLP-GFLFQFIPYMKKYKIQKDKPE ::: :: .. : : . :. :. NP_008 PYVYPATWPEDDIFRQAISLLIVTNVGAYILYF-FCATLSYYFVFDHALMKH------PQ 20 30 40 50 60 90 100 110 120 130 140 pF1KB5 TWENQWKCFKVLLFNHFCIQ-LP---LICGTYYFTE---YFNIPYDWERMPRWYF-LLAR .:: : .: .: :: .. . .. : : .. : ..: : :.. NP_008 FLKNQ-----VRREIKFTVQALPWISILTVALFLLEIRGYSKLHDDLGEFPYGLFELVVS 70 80 90 100 110 120 150 160 170 180 190 200 pF1KB5 CFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFI .. . : . :..:: :::. .:: .:: :: .. : . .. ::.. .. . . : NP_008 IISFLFFTDMFIYWIHRGLHHRLVYKRLHKPHHIWKIPTPFASHAFHPIDGFLQSLPYHI 130 140 150 160 170 180 210 220 230 240 250 260 pF1KB5 GIVLLCDH-VILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFIGN .. : :. : .. . . ::..:.: :. . : : :: :: :: ::: : : NP_008 YPFIFPLHKVVYLSLYILVNIW-TISIHDG-DFRV-PQILQPFINGSAHHTDHHMFFDYN 190 200 210 220 230 270 280 290 pF1KB5 YASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE :.. :: :::: :. .. .... : NP_008 YGQYFTLWDRIGGSFKNPSSFEGKGPLSYVKEMTEGKRSSHSGNGCKNEKLFNGEFTKTE 240 250 260 270 280 290 >>NP_001020127 (OMIM: 602286,607330) lathosterol oxidase (299 aa) initn: 268 init1: 152 opt: 279 Z-score: 364.5 bits: 75.4 E(85289): 1.5e-13 Smith-Waterman score: 279; 29.5% identity (55.1% similar) in 234 aa overlap (61-284:45-263) 40 50 60 70 80 pF1KB5 EPFKNAWNYMLNNYTKFQIATWGSLIVHEALYFLFCLP-GFLFQFIPYMKKYKIQKDKPE ::: :: .. : : . :. :. NP_001 PYVYPATWPEDDIFRQAISLLIVTNVGAYILYF-FCATLSYYFVFDHALMKH------PQ 20 30 40 50 60 90 100 110 120 130 140 pF1KB5 TWENQWKCFKVLLFNHFCIQ-LP---LICGTYYFTE---YFNIPYDWERMPRWYF-LLAR .:: : .: .: :: .. . .. : : .. : ..: : :.. NP_001 FLKNQ-----VRREIKFTVQALPWISILTVALFLLEIRGYSKLHDDLGEFPYGLFELVVS 70 80 90 100 110 120 150 160 170 180 190 200 pF1KB5 CFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFI .. . : . :..:: :::. .:: .:: :: .. : . .. ::.. .. . . : NP_001 IISFLFFTDMFIYWIHRGLHHRLVYKRLHKPHHIWKIPTPFASHAFHPIDGFLQSLPYHI 130 140 150 160 170 180 210 220 230 240 250 260 pF1KB5 GIVLLCDH-VILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFIGN .. : :. : .. . . ::..:.: :. . : : :: :: :: ::: : : NP_001 YPFIFPLHKVVYLSLYILVNIW-TISIHDG-DFRV-PQILQPFINGSAHHTDHHMFFDYN 190 200 210 220 230 270 280 290 pF1KB5 YASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE :.. :: :::: :. .. .... : NP_001 YGQYFTLWDRIGGSFKNPSSFEGKGPLSYVKEMTEGKRSSHSGNGCKNEKLFNGEFTKTE 240 250 260 270 280 290 >>XP_006715794 (OMIM: 613738) PREDICTED: alkylglycerol m (423 aa) initn: 128 init1: 78 opt: 150 Z-score: 195.3 bits: 44.6 E(85289): 0.00039 Smith-Waterman score: 160; 25.4% identity (50.3% similar) in 185 aa overlap (106-284:88-256) 80 90 100 110 120 130 pF1KB5 PYMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEY--FNIPYDWERMP : .. : : . .: ::.: :. XP_006 SWILKGKPPGRLDDALTSISAGVLSRLPSLFFRSIELTSYIYIWENYRLFNLP--WDSPW 60 70 80 90 100 110 140 150 160 170 180 190 pF1KB5 RWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETL :: . .: : .:..::. :. :. :..:: . ... . . . . XP_006 TWY---SAFLGV----DFGYYWFHRMAHEVNIMWAGHQTHHS-SEDYNLSTALRQSVLQI 120 130 140 150 160 200 210 220 230 240 250 pF1KB5 ILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFH . :. ..:. . . . . :: . .:. :.::.:: :: : XP_006 YTSWIFYSPLALFIPPSVYA-VHLQFNLLYQFWIHTEVINNLGPLELI--LNTPSHHRVH 170 180 190 200 210 220 260 270 280 290 pF1KB5 H----MNFIGNYASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE : . . :::... ::.:::: ..: ::: XP_006 HGRNRYCIDKNYAGVLIIWDKIFGT---FEAENEKVVYGLTHPINTFEPIKVQFHHLFSI 230 240 250 260 270 280 XP_006 WTTFWATPGFFNKFSVIFKGPGWGPGKPRLGLSEEIPEVTGKEVPFSSSSSQLLKIYTVV 290 300 310 320 330 340 >>XP_016867693 (OMIM: 613738) PREDICTED: alkylglycerol m (424 aa) initn: 128 init1: 78 opt: 150 Z-score: 195.3 bits: 44.6 E(85289): 0.00039 Smith-Waterman score: 160; 25.4% identity (50.3% similar) in 185 aa overlap (106-284:88-256) 80 90 100 110 120 130 pF1KB5 PYMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEY--FNIPYDWERMP : .. : : . .: ::.: :. XP_016 SWILKGKPPGRLDDALTSISAGVLSRLPSLFFRSIELTSYIYIWENYRLFNLP--WDSPW 60 70 80 90 100 110 140 150 160 170 180 190 pF1KB5 RWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETL :: . .: : .:..::. :. :. :..:: . ... . . . . XP_016 TWY---SAFLGV----DFGYYWFHRMAHEVNIMWAGHQTHHS-SEDYNLSTALRQSVLQI 120 130 140 150 160 200 210 220 230 240 250 pF1KB5 ILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFH . :. ..:. . . . . :: . .:. :.::.:: :: : XP_016 YTSWIFYSPLALFIPPSVYA-VHLQFNLLYQFWIHTEVINNLGPLELI--LNTPSHHRVH 170 180 190 200 210 220 260 270 280 290 pF1KB5 H----MNFIGNYASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE : . . :::... ::.:::: ..: ::: XP_016 HGRNRYCIDKNYAGVLIIWDKIFGT---FEAENEKVVYGLTHPINTFEPIKVQFHHLFSI 230 240 250 260 270 280 XP_016 WTTFWATPGFFNKFSVIFKGPGWGPGKPRLGLSEEIPEVTGKEVPFSSSSSQLLKIYTVV 290 300 310 320 330 340 >>XP_011513704 (OMIM: 613738) PREDICTED: alkylglycerol m (437 aa) initn: 128 init1: 78 opt: 150 Z-score: 195.1 bits: 44.7 E(85289): 0.0004 Smith-Waterman score: 160; 25.4% identity (50.3% similar) in 185 aa overlap (106-284:88-256) 80 90 100 110 120 130 pF1KB5 PYMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEY--FNIPYDWERMP : .. : : . .: ::.: :. XP_011 SWILKGKPPGRLDDALTSISAGVLSRLPSLFFRSIELTSYIYIWENYRLFNLP--WDSPW 60 70 80 90 100 110 140 150 160 170 180 190 pF1KB5 RWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETL :: . .: : .:..::. :. :. :..:: . ... . . . . XP_011 TWY---SAFLGV----DFGYYWFHRMAHEVNIMWAGHQTHHS-SEDYNLSTALRQSVLQI 120 130 140 150 160 200 210 220 230 240 250 pF1KB5 ILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFH . :. ..:. . . . . :: . .:. :.::.:: :: : XP_011 YTSWIFYSPLALFIPPSVYA-VHLQFNLLYQFWIHTEVINNLGPLELI--LNTPSHHRVH 170 180 190 200 210 220 260 270 280 290 pF1KB5 H----MNFIGNYASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE : . . :::... ::.:::: ..: ::: XP_011 HGRNRYCIDKNYAGVLIIWDKIFGT---FEAENEKVVYGLTHPINTFEPIKVQFHHLFSI 230 240 250 260 270 280 XP_011 WTTFWATPGFFNKFSVIFKGPGWGPGKPRLGLSEEIPEVTGKEVPFSSSSSQLLKIYTVV 290 300 310 320 330 340 >>NP_001004320 (OMIM: 613738) alkylglycerol monooxygenas (445 aa) initn: 128 init1: 78 opt: 150 Z-score: 195.0 bits: 44.7 E(85289): 0.0004 Smith-Waterman score: 160; 25.4% identity (50.3% similar) in 185 aa overlap (106-284:88-256) 80 90 100 110 120 130 pF1KB5 PYMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEY--FNIPYDWERMP : .. : : . .: ::.: :. NP_001 SWILKGKPPGRLDDALTSISAGVLSRLPSLFFRSIELTSYIYIWENYRLFNLP--WDSPW 60 70 80 90 100 110 140 150 160 170 180 190 pF1KB5 RWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETL :: . .: : .:..::. :. :. :..:: . ... . . . . NP_001 TWY---SAFLGV----DFGYYWFHRMAHEVNIMWAGHQTHHS-SEDYNLSTALRQSVLQI 120 130 140 150 160 200 210 220 230 240 250 pF1KB5 ILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFH . :. ..:. . . . . :: . .:. :.::.:: :: : NP_001 YTSWIFYSPLALFIPPSVYA-VHLQFNLLYQFWIHTEVINNLGPLELI--LNTPSHHRVH 170 180 190 200 210 220 260 270 280 290 pF1KB5 H----MNFIGNYASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE : . . :::... ::.:::: ..: ::: NP_001 HGRNRYCIDKNYAGVLIIWDKIFGT---FEAENEKVVYGLTHPINTFEPIKVQFHHLFSI 230 240 250 260 270 280 NP_001 WTTFWATPGFFNKFSVIFKGPGWGPGKPRLGLSEEIPEVTGKEVPFSSSSSQLLKIYTVV 290 300 310 320 330 340 293 residues in 1 query sequences 60827320 residues in 85289 library sequences Tcomplib [36.3.4 Apr, 2011] (8 proc) start: Sat Nov 5 06:30:02 2016 done: Sat Nov 5 06:30:03 2016 Total Scan time: 6.560 Total Display time: 0.020 Function used was FASTA [36.3.4 Apr, 2011]