Restriction map for
pF1KB5231
(Circular) (Six-base) MAPSORT of: pF1KB5231.seq Check: 5408 from: 1 to: 5243
pF1KB5231 5243 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 393 393
Size: 5243
AccI GT'mk_AC
Cuts at: 1952 2281 1952
Size: 329 4914
Acc65I G'GTAC_C
Cuts at: 2259 2259
Size: 5243
AclI AA'CG_TT
Cuts at: 5083 5083
Size: 5243
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 746 1001 1199 1597 1880 3068 3500 3853 4515
Size: 255 198 398 283 1188 432 353 662
Cuts at: 4515 746
Size: 1474
Fragments arranged by size:
1474 1188 662 432 398 353 283 255
198
AflIII A'CryG_T
Cuts at: 2595 4395 4676 2595
Size: 1800 281 3162
Fragments arranged by size:
3162 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 643 646 979 1426 2548 3986 643
Size: 3 333 447 1122 1438 1900
Fragments arranged by size:
1900 1438 1122 447 333 3
ApaLI G'TGCA_C
Cuts at: 4081 4081
Size: 5243
ApoI r'AATT_y
Cuts at: 1150 1411 1728 2247 4596 4852 1150
Size: 261 317 519 2349 256 1541
Fragments arranged by size:
2349 1541 519 317 261 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5243
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5243
AvaI C'yCGr_G
Cuts at: 2263 2263
Size: 5243
BamHI G'GATC_C
Cuts at: 2268 2268
Size: 5243
BanI G'GyrC_C
Cuts at: 2259 2673 2921 2956 2259
Size: 414 248 35 4546
Fragments arranged by size:
4546 414 248 35
BanII G_rGCy'C
Cuts at: 2257 3287 2257
Size: 1030 4213
BbeI G_GCGC'C
Cuts at: 2677 2925 2677
Size: 248 4995
BbsI GAAGACnn'nnnn_
Cuts at: 400 542 1187 1715 2006 2230 400
Size: 142 645 528 291 224 3413
Fragments arranged by size:
3413 645 528 291 224 142
BbvCI CC'TCA_GC
Cuts at: 285 806 285
Size: 521 4722
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1081 1115 1081
Size: 34 5209
BciVI GTATCCnnnnn_n'
Cuts at: 1845 3141 4197 5243 1845
Size: 1296 1056 1046 1845
Fragments arranged by size:
1845 1296 1056 1046
BclI T'GATC_A
Cuts at: 2763 2763
Size: 5243
BfrBI ATG'CAT
Cuts at: 1684 1684
Size: 5243
BglI GCCn_nnn'nGGC
Cuts at: 505 1990 2153 505
Size: 1485 163 3595
Fragments arranged by size:
3595 1485 163
BglII A'GATC_T
Cuts at: 2758 4795 2758
Size: 2037 3206
BlpI GC'TnA_GC
Cuts at: 2355 2355
Size: 5243
Bme1580I G_kGCm'C
Cuts at: 2868 2961 4085 2868
Size: 93 1124 4026
Fragments arranged by size:
4026 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1934 2046 2455 2536 2871 5015 1934
Size: 112 409 81 335 2144 2162
Fragments arranged by size:
2162 2144 409 335 112 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3610 3610
Size: 5243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 344 1207 1520 1792 344
Size: 863 313 272 3795
Fragments arranged by size:
3795 863 313 272
Bpu10I CC'TnA_GC
Cuts at: 285 806 285
Size: 521 4722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2419 3298 4012 4310 2419
Size: 879 714 298 3352
Fragments arranged by size:
3352 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4947 4947
Size: 5243
BsaAI yAC'GTr
Cuts at: 2596 2596
Size: 5243
BsaBI GATnn'nnATC
Cuts at: 6 2757 4794 6
Size: 2751 2037 455
Fragments arranged by size:
2751 2037 455
BsaHI Gr'CG_yC
Cuts at: 2674 2922 3624 5120 2674
Size: 248 702 1496 2797
Fragments arranged by size:
2797 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2432 2953 3631 4042 4189 4463 2432
Size: 521 678 411 147 274 3212
Fragments arranged by size:
3212 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 227 257 1180 1210 227
Size: 30 923 30 4260
Fragments arranged by size:
4260 923 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 152 155 565 583 592 907 910 922 925
Size: 3 410 18 9 315 3 12 3
Cuts at: 925 1981 2132 2141 2183 2195 2204 2207 2210
Size: 1056 151 9 42 12 9 3 3
Cuts at: 2210 152
Size: 3185
Fragments arranged by size:
3185 1056 410 315 151 42 18 12
12 9 9 9 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 495 2668 3426 4091 4499 495
Size: 2173 758 665 408 1239
Fragments arranged by size:
2173 1239 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2785 2831 4061 104
Size: 2681 46 1230 1286
Fragments arranged by size:
2681 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2257 3035 3225 4085 2257
Size: 778 190 860 3415
Fragments arranged by size:
3415 860 778 190
BsmI GAATG_Cn'
Cuts at: 528 528
Size: 5243
Bsp1286I G_dGCh'C
Cuts at: 2257 2868 2961 3035 3225 3287 4085 2257
Size: 611 93 74 190 62 798 3415
Fragments arranged by size:
3415 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 3675 3675
Size: 5243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 118 393 2293 2507 2809 3190 118
Size: 275 1900 214 302 381 2171
Fragments arranged by size:
2171 1900 381 302 275 214
BsrBI CCG'CTC
Cuts at: 50 3535 3589 5073 5238 50
Size: 3485 54 1484 165 55
Fragments arranged by size:
3485 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1233 3155 1233
Size: 1922 3321
BsrFI r'CCGG_y
Cuts at: 3241 3422 3241
Size: 181 5062
BssSI C'ACGA_G
Cuts at: 3514 4222 3514
Size: 708 4535
BstAPI GCAn_nnn'nTGC
Cuts at: 385 643 646 2620 385
Size: 258 3 1974 3008
Fragments arranged by size:
3008 1974 258 3
BstBI TT'CG_AA
Cuts at: 1706 3604 1706
Size: 1898 3345
BstEII G'GTnAC_C
Cuts at: 142 2200 142
Size: 2058 3185
BstXI CCAn_nnnn'nTGG
Cuts at: 156 801 156
Size: 645 4598
BstYI r'GATC_y
Cuts at: 1 2268 2735 2758 3093 3339 3645 3657 3743
Size: 2267 467 23 335 246 306 12 86
Cuts at: 3743 4795 1
Size: 1052 449
Fragments arranged by size:
2267 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 2048 2048
Size: 5243
BtgI C'CryG_G
Cuts at: 107 1994 107
Size: 1887 3356
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 643 1106 3193 3374 3537 643
Size: 463 2087 181 163 2349
Fragments arranged by size:
2349 2087 463 181 163
BtsI GCAGTG_nn'
Cuts at: 237 2486 237
Size: 2249 2994
ClaI AT'CG_AT
Cuts at: 1469 1469
Size: 5243
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 278 313 278
Size: 35 5208
DraI TTT'AAA
Cuts at: 1727 2242 1727
Size: 515 4728
DrdI GACnn_nn'nnGTC
Cuts at: 2949 4293 2949
Size: 1344 3899
EaeI y'GGCC_r
Cuts at: 959 2828 3002 3393 3420 4765 959
Size: 1869 174 391 27 1345 1437
Fragments arranged by size:
1869 1437 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2828 2828
Size: 5243
EarI CTCTTCn'nnn_
Cuts at: 233 542 818 899 911 1616 1856 3266 3476
Size: 309 276 81 12 705 240 1410 210
Cuts at: 3476 233
Size: 2000
Fragments arranged by size:
2000 1410 705 309 276 240 210 81
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3451 4193 4339 5053 3451
Size: 742 146 714 3641
Fragments arranged by size:
3641 742 714 146
EcoICRI GAG'CTC
Cuts at: 2255 2255
Size: 5243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 344 746 1001 1199 1207 1520 1597 1792 1880
Size: 402 255 198 8 313 77 195 88
Cuts at: 1880 3068 3500 3853 4515 344
Size: 1188 432 353 662 1072
Fragments arranged by size:
1188 1072 662 432 402 353 313 255
198 195 88 77 8
EcoNI CCTnn'n_nnAGG
Cuts at: 1846 1846
Size: 5243
EcoO109I rG'GnC_Cy
Cuts at: 1783 1928 2382 1783
Size: 145 454 4644
Fragments arranged by size:
4644 454 145
EcoRI G'AATT_C
Cuts at: 2247 2247
Size: 5243
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1627 3262 1627
Size: 1635 3608
FspI TGC'GCA
Cuts at: 3024 3024
Size: 5243
HaeII r_GCGC'y
Cuts at: 2677 2925 4155 4926 2677
Size: 248 1230 771 2994
Fragments arranged by size:
2994 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 190 222 227 259 1156 1188 1462 1494 3085
Size: 32 5 32 897 32 274 32 1591
Cuts at: 3085 3115 3117 3147 190
Size: 30 2 30 2286
Fragments arranged by size:
2286 1591 897 274 32 32 32 32
30 30 5 2
HincII GTy'rAC
Cuts at: 2282 2282
Size: 5243
HindIII A'AGCT_T
Cuts at: 1721 1721
Size: 5243
KasI G'GCGC_C
Cuts at: 2673 2921 2673
Size: 248 4995
KpnI G_GTAC'C
Cuts at: 2263 2263
Size: 5243
MluI A'CGCG_T
Cuts at: 4676 4676
Size: 5243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1020 1178 4002 4186 1020
Size: 158 2824 184 2077
Fragments arranged by size:
2824 2077 184 158
MscI TGG'CCA
Cuts at: 961 3004 961
Size: 2043 3200
MslI CAynn'nnrTG
Cuts at: 3232 3359 3232
Size: 127 5116
MspA1I CmG'CkG
Cuts at: 352 643 2354 2668 3028 3812 4057 352
Size: 291 1711 314 360 784 245 1538
Fragments arranged by size:
1711 1538 784 360 314 291 245
NaeI GCC'GGC
Cuts at: 3424 3424
Size: 5243
NarI GG'CG_CC
Cuts at: 2674 2922 2674
Size: 248 4995
NcoI C'CATG_G
Cuts at: 107 1994 107
Size: 1887 3356
NgoMIV G'CCGG_C
Cuts at: 3422 3422
Size: 5243
NruI TCG'CGA
Cuts at: 121 121
Size: 5243
NsiI A_TGCA'T
Cuts at: 1686 1686
Size: 5243
NspI r_CATG'y
Cuts at: 2296 2796 4399 2296
Size: 500 1603 3140
Fragments arranged by size:
3140 1603 500
PasI CC'CwG_GG
Cuts at: 1787 1787
Size: 5243
PciI A'CATG_T
Cuts at: 4395 4395
Size: 5243
PmeI GTTT'AAAC
Cuts at: 2242 2242
Size: 5243
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 3689
Size: 32 5211
PpuMI rG'GwC_Cy
Cuts at: 1783 1928 1783
Size: 145 5098
PstI C_TGCA'G
Cuts at: 2290 2975 2290
Size: 685 4558
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5243
PvuII CAG'CTG
Cuts at: 352 643 2668 3028 352
Size: 291 2025 360 2567
Fragments arranged by size:
2567 2025 360 291
RsrII CG'GwC_CG
Cuts at: 3438 3438
Size: 5243
SacI G_AGCT'C
Cuts at: 2257 2257
Size: 5243
SalI G'TCGA_C
Cuts at: 2280 2280
Size: 5243
SapI GCTCTTCn'nnn_
Cuts at: 911 3266 3476 911
Size: 2355 210 2678
Fragments arranged by size:
2678 2355 210
SbfI CC_TGCA'GG
Cuts at: 2290 2290
Size: 5243
ScaI AGT'ACT
Cuts at: 4760 4760
Size: 5243
SfcI C'TryA_G
Cuts at: 33 1524 1590 2286 2971 3939 4130 33
Size: 1491 66 696 685 968 191 1146
Fragments arranged by size:
1491 1146 968 696 685 191 66
SfoI GGC'GCC
Cuts at: 2675 2923 2675
Size: 248 4995
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5243
SmaI CCC'GGG
Cuts at: 2265 2265
Size: 5243
SmlI C'TyrA_G
Cuts at: 2398 3313 4027 4289 2398
Size: 915 714 262 3352
Fragments arranged by size:
3352 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5243
SphI G_CATG'C
Cuts at: 2296 2796 2296
Size: 500 4743
StuI AGG'CCT
Cuts at: 1841 1841
Size: 5243
StyI C'CwwG_G
Cuts at: 107 500 1985 1994 2030 2377 2731 107
Size: 393 1485 9 36 347 354 2619
Fragments arranged by size:
2619 1485 393 354 347 36 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2826 2959 3418 3627 2826
Size: 133 459 209 4442
Fragments arranged by size:
4442 459 209 133
TatI w'GTAC_w
Cuts at: 1490 4758 1490
Size: 3268 1975
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4760 4792 4760
Size: 32 5211
Tth111I GACn'n_nGTC
Cuts at: 3040 3040
Size: 5243
XbaI T'CTAG_A
Cuts at: 2274 2274
Size: 5243
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1660 1992 2738 1660
Size: 332 746 4165
Fragments arranged by size:
4165 746 332
XmaI C'CCGG_G
Cuts at: 2263 2263
Size: 5243
XmnI GAAnn'nnTTC
Cuts at: 1610 1610
Size: 5243
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NsiI NspI PasI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BmgBI BmtI BsgI BsiWI
BsmBI BspEI BsrGI BssHII BstZ17I DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI PacI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI