Restriction map for
pF1KB5234
(Circular) (Six-base) MAPSORT of: pF1KB5234.seq Check: 5400 from: 1 to: 4715
pF1KB5234 4715 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 574 1753 574
Size: 1179 3536
Acc65I G'GTAC_C
Cuts at: 968 1731 968
Size: 763 3952
AclI AA'CG_TT
Cuts at: 4555 4555
Size: 4715
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 2540 2972 3325 3987 611
Size: 1929 432 353 662 1339
Fragments arranged by size:
1929 1339 662 432 353
AfeI AGC'GCT
Cuts at: 646 1171 646
Size: 525 4190
AflIII A'CryG_T
Cuts at: 1571 2067 3867 4148 1571
Size: 496 1800 281 2138
Fragments arranged by size:
2138 1800 496 281
AhdI GACnn_n'nnGTC
Cuts at: 1623 1623
Size: 4715
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 814 814
Size: 4715
AlwNI CAG_nnn'CTG
Cuts at: 286 535 1242 1383 2020 3458 286
Size: 249 707 141 637 1438 1543
Fragments arranged by size:
1543 1438 707 637 249 141
ApaI G_GGCC'C
Cuts at: 399 714 399
Size: 315 4400
ApaLI G'TGCA_C
Cuts at: 1090 3553 1090
Size: 2463 2252
ApoI r'AATT_y
Cuts at: 1719 4068 4324 1719
Size: 2349 256 2110
Fragments arranged by size:
2349 2110 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4715
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4715
AvaI C'yCGr_G
Cuts at: 488 752 1514 1735 488
Size: 264 762 221 3468
Fragments arranged by size:
3468 762 264 221
BamHI G'GATC_C
Cuts at: 1740 1740
Size: 4715
BanI G'GyrC_C
Cuts at: 131 968 1731 2145 2393 2428 131
Size: 837 763 414 248 35 2418
Fragments arranged by size:
2418 837 763 414 248 35
BanII G_rGCy'C
Cuts at: 124 399 521 714 1523 1729 2759 124
Size: 275 122 193 809 206 1030 2080
Fragments arranged by size:
2080 1030 809 275 206 193 122
BbeI G_GCGC'C
Cuts at: 135 2149 2397 135
Size: 2014 248 2453
Fragments arranged by size:
2453 2014 248
BbvCI CC'TCA_GC
Cuts at: 1058 1058
Size: 4715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 369 403 369
Size: 34 4681
BciVI GTATCCnnnnn_n'
Cuts at: 2613 3669 4715 2613
Size: 1056 1046 2613
Fragments arranged by size:
2613 1056 1046
BclI T'GATC_A
Cuts at: 1334 2235 1334
Size: 901 3814
BglII A'GATC_T
Cuts at: 665 2230 4267 665
Size: 1565 2037 1113
Fragments arranged by size:
2037 1565 1113
BlpI GC'TnA_GC
Cuts at: 287 1827 287
Size: 1540 3175
Bme1580I G_kGCm'C
Cuts at: 399 714 1094 2340 2433 3557 399
Size: 315 380 1246 93 1124 1557
Fragments arranged by size:
1557 1246 1124 380 315 93
BmrI ACTGGGnnnn_n'
Cuts at: 1927 2008 2343 4487 1927
Size: 81 335 2144 2155
Fragments arranged by size:
2155 2144 335 81
BmtI G_CTAG'C
Cuts at: 1531 1531
Size: 4715
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1613 3082 1613
Size: 1469 3246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1027
Size: 4715
Bpu10I CC'TnA_GC
Cuts at: 1058 1058
Size: 4715
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1150 1184 1891 2770 3484 3782 1150
Size: 34 707 879 714 298 2083
Fragments arranged by size:
2083 879 714 707 298 34
BsaI GGTCTCn'nnnn_
Cuts at: 4419 4419
Size: 4715
BsaAI yAC'GTr
Cuts at: 342 2068 342
Size: 1726 2989
BsaBI GATnn'nnATC
Cuts at: 6 1501 2229 4266 6
Size: 1495 728 2037 455
Fragments arranged by size:
2037 1495 728 455
BsaHI Gr'CG_yC
Cuts at: 132 2146 2394 3096 4592 132
Size: 2014 248 702 1496 255
Fragments arranged by size:
2014 1496 702 255 248
BsaWI w'CCGG_w
Cuts at: 812 1904 2425 3103 3514 3661 3935 812
Size: 1092 521 678 411 147 274 1592
Fragments arranged by size:
1592 1092 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 185 238 604 185
Size: 53 366 4296
Fragments arranged by size:
4296 366 53
BseYI C'CCAG_C
Cuts at: 296 1122 1523 2140 2898 3563 3971 296
Size: 826 401 617 758 665 408 1040
Fragments arranged by size:
1040 826 758 665 617 408 401
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 478
Size: 4715
BsiEI CG_ry'CG
Cuts at: 104 2257 2303 3533 104
Size: 2153 46 1230 1286
Fragments arranged by size:
2153 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 293 341 1094 1729 2507 2697 3557 293
Size: 48 753 635 778 190 860 1451
Fragments arranged by size:
1451 860 778 753 635 190 48
Bsp1286I G_dGCh'C
Cuts at: 124 293 341 399 521 714 1094 1523 1729
Size: 169 48 58 122 193 380 429 206
Cuts at: 1729 2340 2433 2507 2697 2759 3557 124
Size: 611 93 74 190 62 798 1282
Fragments arranged by size:
1282 798 611 429 380 206 193 190
169 122 93 74 62 58 48
BspEI T'CCGG_A
Cuts at: 812 812
Size: 4715
BspHI T'CATG_A
Cuts at: 629 3147 629
Size: 2518 2197
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1765 1979 2281 2662 1765
Size: 214 302 381 3818
Fragments arranged by size:
3818 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3007 3061 4545 4710 50
Size: 2957 54 1484 165 55
Fragments arranged by size:
2957 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2627 2627
Size: 4715
BsrFI r'CCGG_y
Cuts at: 195 1139 2713 2894 195
Size: 944 1574 181 2016
Fragments arranged by size:
2016 1574 944 181
BssSI C'ACGA_G
Cuts at: 616 1465 2986 3694 616
Size: 849 1521 708 1637
Fragments arranged by size:
1637 1521 849 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2092 2092
Size: 4715
BstBI TT'CG_AA
Cuts at: 3076 3076
Size: 4715
BstXI CCAn_nnnn'nTGG
Cuts at: 1005 1680 1005
Size: 675 4040
BstYI r'GATC_y
Cuts at: 1 665 1130 1740 2207 2230 2565 2811 3117
Size: 664 465 610 467 23 335 246 306
Cuts at: 3117 3129 3215 4267 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 664 610 467 465 449 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 135 521 135
Size: 386 4329
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1356 2665 2846 3009 1356
Size: 1309 181 163 3062
Fragments arranged by size:
3062 1309 181 163
BtsI GCAGTG_nn'
Cuts at: 1958 1958
Size: 4715
DraI TTT'AAA
Cuts at: 1714 1714
Size: 4715
DraIII CAC_nnn'GTG
Cuts at: 1311 1311
Size: 4715
DrdI GACnn_nn'nnGTC
Cuts at: 2421 3765 2421
Size: 1344 3371
EaeI y'GGCC_r
Cuts at: 2300 2474 2865 2892 4237 2300
Size: 174 391 27 1345 2778
Fragments arranged by size:
2778 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2300 2300
Size: 4715
EarI CTCTTCn'nnn_
Cuts at: 509 2738 2948 509
Size: 2229 210 2276
Fragments arranged by size:
2276 2229 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 182 1141 2923 3665 3811 4525 182
Size: 959 1782 742 146 714 372
Fragments arranged by size:
1782 959 742 714 372 146
EcoICRI GAG'CTC
Cuts at: 1727 1727
Size: 4715
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 1027 2540 2972 3325 3987 611
Size: 416 1513 432 353 662 1339
Fragments arranged by size:
1513 1339 662 432 416 353
EcoNI CCTnn'n_nnAGG
Cuts at: 1048 1048
Size: 4715
EcoO109I rG'GnC_Cy
Cuts at: 396 711 1053 1239 1277 1854 396
Size: 315 342 186 38 577 3257
Fragments arranged by size:
3257 577 342 315 186 38
EcoRI G'AATT_C
Cuts at: 1719 1719
Size: 4715
EcoRV GAT'ATC
Cuts at: 1563 1563
Size: 4715
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4715
FspI TGC'GCA
Cuts at: 790 2496 790
Size: 1706 3009
HaeII r_GCGC'y
Cuts at: 135 648 1173 2149 2397 3627 4398 135
Size: 513 525 976 248 1230 771 452
Fragments arranged by size:
1230 976 771 525 513 452 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 681 713 1609 1641 2557 2587 2589 2619 681
Size: 32 896 32 916 30 2 30 2777
Fragments arranged by size:
2777 916 896 32 32 30 30 2
HincII GTy'rAC
Cuts at: 248 575 1754 248
Size: 327 1179 3209
Fragments arranged by size:
3209 1179 327
HindIII A'AGCT_T
Cuts at: 1096 1096
Size: 4715
KasI G'GCGC_C
Cuts at: 131 2145 2393 131
Size: 2014 248 2453
Fragments arranged by size:
2453 2014 248
KpnI G_GTAC'C
Cuts at: 972 1735 972
Size: 763 3952
MluI A'CGCG_T
Cuts at: 4148 4148
Size: 4715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 633 1370 3474 3658 633
Size: 737 2104 184 1690
Fragments arranged by size:
2104 1690 737 184
MscI TGG'CCA
Cuts at: 2476 2476
Size: 4715
MslI CAynn'nnrTG
Cuts at: 628 1108 2704 2831 628
Size: 480 1596 127 2512
Fragments arranged by size:
2512 1596 480 127
MspA1I CmG'CkG
Cuts at: 137 283 1005 1069 1826 2140 2500 3284 3529
Size: 146 722 64 757 314 360 784 245
Cuts at: 3529 137
Size: 1323
Fragments arranged by size:
1323 784 757 722 360 314 245 146
64
NaeI GCC'GGC
Cuts at: 197 2896 197
Size: 2699 2016
NarI GG'CG_CC
Cuts at: 132 2146 2394 132
Size: 2014 248 2453
Fragments arranged by size:
2453 2014 248
NcoI C'CATG_G
Cuts at: 521 521
Size: 4715
NgoMIV G'CCGG_C
Cuts at: 195 2894 195
Size: 2699 2016
NheI G'CTAG_C
Cuts at: 1527 1527
Size: 4715
NspI r_CATG'y
Cuts at: 805 1575 1768 2268 3871 805
Size: 770 193 500 1603 1649
Fragments arranged by size:
1649 1603 770 500 193
PciI A'CATG_T
Cuts at: 1571 3867 1571
Size: 2296 2419
PflMI CCAn_nnn'nTGG
Cuts at: 210 1242 210
Size: 1032 3683
PfoI T'CCnGG_A
Cuts at: 509 767 1241 1433 509
Size: 258 474 192 3791
Fragments arranged by size:
3791 474 258 192
PmeI GTTT'AAAC
Cuts at: 1714 1714
Size: 4715
PmlI CAC'GTG
Cuts at: 342 342
Size: 4715
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 4683
PpuMI rG'GwC_Cy
Cuts at: 1239 1277 1239
Size: 38 4677
PspOMI G'GGCC_C
Cuts at: 395 710 395
Size: 315 4400
PstI C_TGCA'G
Cuts at: 1148 1388 1632 1762 2447 1148
Size: 240 244 130 685 3416
Fragments arranged by size:
3416 685 244 240 130
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4715
PvuII CAG'CTG
Cuts at: 1005 1069 2140 2500 1005
Size: 64 1071 360 3220
Fragments arranged by size:
3220 1071 360 64
RsrII CG'GwC_CG
Cuts at: 2910 2910
Size: 4715
SacI G_AGCT'C
Cuts at: 1729 1729
Size: 4715
SacII CC_GC'GG
Cuts at: 138 138
Size: 4715
SalI G'TCGA_C
Cuts at: 573 1752 573
Size: 1179 3536
SapI GCTCTTCn'nnn_
Cuts at: 509 2738 2948 509
Size: 2229 210 2276
Fragments arranged by size:
2276 2229 210
SbfI CC_TGCA'GG
Cuts at: 1762 1762
Size: 4715
ScaI AGT'ACT
Cuts at: 696 1012 4232 696
Size: 316 3220 1179
Fragments arranged by size:
3220 1179 316
SexAI A'CCwGG_T
Cuts at: 1235 1235
Size: 4715
SfcI C'TryA_G
Cuts at: 33 888 1144 1384 1628 1758 2443 3411 3602
Size: 855 256 240 244 130 685 968 191
Cuts at: 3602 33
Size: 1146
Fragments arranged by size:
1146 968 855 685 256 244 240 191
130
SfoI GGC'GCC
Cuts at: 133 2147 2395 133
Size: 2014 248 2453
Fragments arranged by size:
2453 2014 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4715
SmaI CCC'GGG
Cuts at: 754 1737 754
Size: 983 3732
SmlI C'TyrA_G
Cuts at: 488 1165 1199 1870 2785 3499 3761 488
Size: 677 34 671 915 714 262 1442
Fragments arranged by size:
1442 915 714 677 671 262 34
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4715
SphI G_CATG'C
Cuts at: 805 1768 2268 805
Size: 963 500 3252
Fragments arranged by size:
3252 963 500
StyI C'CwwG_G
Cuts at: 320 521 1361 1424 1487 1673 1849 2203 320
Size: 201 840 63 63 186 176 354 2832
Fragments arranged by size:
2832 840 354 201 186 176 63 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2298 2431 2890 3099 2298
Size: 133 459 209 3914
Fragments arranged by size:
3914 459 209 133
TatI w'GTAC_w
Cuts at: 694 1010 4230 694
Size: 316 3220 1179
Fragments arranged by size:
3220 1179 316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4232 4264 4232
Size: 32 4683
Tth111I GACn'n_nGTC
Cuts at: 313 2512 313
Size: 2199 2516
XbaI T'CTAG_A
Cuts at: 1746 1746
Size: 4715
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2210 2210
Size: 4715
XhoI C'TCGA_G
Cuts at: 488 488
Size: 4715
XmaI C'CCGG_G
Cuts at: 752 1735 752
Size: 983 3732
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AleI AloI AscI AvrII
BaeI BbsI BfrBI BglI BmgBI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
FseI FspAI HpaI MfeI NdeI NotI NruI NsiI
PacI PasI PshAI PsiI PspXI PsrI SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI