Restriction map for
pF1KB5236
(Circular) (Six-base) MAPSORT of: pF1KB5236.seq Check: 3307 from: 1 to: 4175
pF1KB5236 4175 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 117 1148 1213 117
Size: 1031 65 3079
Fragments arranged by size:
3079 1031 65
Acc65I G'GTAC_C
Cuts at: 1191 1191
Size: 4175
AclI AA'CG_TT
Cuts at: 1169 4015 1169
Size: 2846 1329
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 1140 2000 2432 3179 3447 469
Size: 671 860 432 747 268 1197
Fragments arranged by size:
1197 860 747 671 432 268
AflIII A'CryG_T
Cuts at: 1527 2631 3608 1527
Size: 1104 977 2094
Fragments arranged by size:
2094 1104 977
AgeI A'CCGG_T
Cuts at: 2563 2563
Size: 4175
AleI CACnn'nnGTG
Cuts at: 266 266
Size: 4175
AlwNI CAG_nnn'CTG
Cuts at: 1480 3047 1480
Size: 1567 2608
ApaLI G'TGCA_C
Cuts at: 2945 2945
Size: 4175
ApoI r'AATT_y
Cuts at: 292 998 1179 3528 3784 292
Size: 706 181 2349 256 683
Fragments arranged by size:
2349 706 683 256 181
AseI AT'TA_AT
Cuts at: 20 20
Size: 4175
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
AvaI C'yCGr_G
Cuts at: 1195 1195
Size: 4175
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 542 575 542
Size: 33 4142
BamHI G'GATC_C
Cuts at: 1200 1200
Size: 4175
BanI G'GyrC_C
Cuts at: 1191 1605 1853 1888 1191
Size: 414 248 35 3478
Fragments arranged by size:
3478 414 248 35
BanII G_rGCy'C
Cuts at: 782 1189 2219 782
Size: 407 1030 2738
Fragments arranged by size:
2738 1030 407
BbeI G_GCGC'C
Cuts at: 1609 1857 1609
Size: 248 3927
BciVI GTATCCnnnnn_n'
Cuts at: 397 2073 2834 4175 397
Size: 1676 761 1341 397
Fragments arranged by size:
1676 1341 761 397
BclI T'GATC_A
Cuts at: 153 1695 153
Size: 1542 2633
BglI GCCn_nnn'nGGC
Cuts at: 1074 1074
Size: 4175
BglII A'GATC_T
Cuts at: 1690 3727 1690
Size: 2037 2138
BlpI GC'TnA_GC
Cuts at: 1287 1287
Size: 4175
Bme1580I G_kGCm'C
Cuts at: 806 1800 1893 2949 806
Size: 994 93 1056 2032
Fragments arranged by size:
2032 1056 994 93
BmrI ACTGGGnnnn_n'
Cuts at: 616 1387 1468 1803 3947 616
Size: 771 81 335 2144 844
Fragments arranged by size:
2144 844 771 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 4175
Bpu10I CC'TnA_GC
Cuts at: 321 321
Size: 4175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 1351 2230 2722 3020 358
Size: 993 879 492 298 1513
Fragments arranged by size:
1513 993 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 296 3879 296
Size: 3583 592
BsaAI yAC'GTr
Cuts at: 1528 1528
Size: 4175
BsaBI GATnn'nnATC
Cuts at: 6 139 1689 3726 6
Size: 133 1550 2037 455
Fragments arranged by size:
2037 1550 455 133
BsaHI Gr'CG_yC
Cuts at: 695 1606 1854 2556 4052 695
Size: 911 248 702 1496 818
Fragments arranged by size:
1496 911 818 702 248
BsaWI w'CCGG_w
Cuts at: 1364 1885 2563 2837 2984 3395 1364
Size: 521 678 274 147 411 2144
Fragments arranged by size:
2144 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 146 1600 2358 2935 3431 146
Size: 1454 758 577 496 890
Fragments arranged by size:
1454 890 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1717 1763 2971 104
Size: 1613 46 1208 1308
Fragments arranged by size:
1613 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 960 1189 1967 2157 2949 960
Size: 229 778 190 792 2186
Fragments arranged by size:
2186 792 778 229 190
Bsp1286I G_dGCh'C
Cuts at: 782 806 960 1189 1800 1893 1967 2157 2219
Size: 24 154 229 611 93 74 190 62
Cuts at: 2219 2949 782
Size: 730 2008
Fragments arranged by size:
2008 730 611 229 190 154 93 74
62 24
BspEI T'CCGG_A
Cuts at: 3395 3395
Size: 4175
BspHI T'CATG_A
Cuts at: 183 3351 183
Size: 3168 1007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1225 1439 1741 2122 1225
Size: 214 302 381 3278
Fragments arranged by size:
3278 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2467 2521 4005 4170 50
Size: 2417 54 1484 165 55
Fragments arranged by size:
2417 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 279 729 2087 279
Size: 450 1358 2367
Fragments arranged by size:
2367 1358 450
BsrFI r'CCGG_y
Cuts at: 727 2173 2354 2563 727
Size: 1446 181 209 2339
Fragments arranged by size:
2339 1446 209 181
BssSI C'ACGA_G
Cuts at: 631 2446 2804 631
Size: 1815 358 2002
Fragments arranged by size:
2002 1815 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1552 1552
Size: 4175
BstBI TT'CG_AA
Cuts at: 2536 2536
Size: 4175
BstYI r'GATC_y
Cuts at: 1 1200 1667 1690 2025 2271 3283 3369 3381
Size: 1199 467 23 335 246 1012 86 12
Cuts at: 3381 3727 1
Size: 346 449
Fragments arranged by size:
1199 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 398 398
Size: 4175
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2125 2306 2469 2125
Size: 181 163 3831
Fragments arranged by size:
3831 181 163
BtsI GCAGTG_nn'
Cuts at: 280 465 1106 1418 280
Size: 185 641 312 3037
Fragments arranged by size:
3037 641 312 185
DraI TTT'AAA
Cuts at: 1174 1174
Size: 4175
DrdI GACnn_nn'nnGTC
Cuts at: 1881 2739 1881
Size: 858 3317
EaeI y'GGCC_r
Cuts at: 882 1760 1934 2325 2352 3697 882
Size: 878 174 391 27 1345 1360
Fragments arranged by size:
1360 1345 878 391 174 27
EagI C'GGCC_G
Cuts at: 1760 1760
Size: 4175
EarI CTCTTCn'nnn_
Cuts at: 106 2198 2408 106
Size: 2092 210 1873
Fragments arranged by size:
2092 1873 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1051 2383 2693 2839 3985 1051
Size: 1332 310 146 1146 1241
Fragments arranged by size:
1332 1241 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1187 1187
Size: 4175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 1140 2000 2432 3179 3447 469
Size: 671 860 432 747 268 1197
Fragments arranged by size:
1197 860 747 671 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1314 1314
Size: 4175
EcoRI G'AATT_C
Cuts at: 1179 1179
Size: 4175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 4175
FspI TGC'GCA
Cuts at: 1956 1956
Size: 4175
HaeII r_GCGC'y
Cuts at: 615 1080 1609 1857 2879 3858 615
Size: 465 529 248 1022 979 932
Fragments arranged by size:
1022 979 932 529 465 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2017 2047 2049 2079 2017
Size: 30 2 30 4113
Fragments arranged by size:
4113 30 30 2
HincII GTy'rAC
Cuts at: 1214 1214
Size: 4175
HindIII A'AGCT_T
Cuts at: 938 938
Size: 4175
KasI G'GCGC_C
Cuts at: 1605 1853 1605
Size: 248 3927
KpnI G_GTAC'C
Cuts at: 1195 1195
Size: 4175
MluI A'CGCG_T
Cuts at: 3608 3608
Size: 4175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2846 3030 2846
Size: 184 3991
MscI TGG'CCA
Cuts at: 1936 1936
Size: 4175
MslI CAynn'nnrTG
Cuts at: 266 285 2164 2291 266
Size: 19 1879 127 2150
Fragments arranged by size:
2150 1879 127 19
MspA1I CmG'CkG
Cuts at: 687 1016 1286 1600 1960 2973 3218 687
Size: 329 270 314 360 1013 245 1644
Fragments arranged by size:
1644 1013 360 329 314 270 245
NaeI GCC'GGC
Cuts at: 729 2356 729
Size: 1627 2548
NarI GG'CG_CC
Cuts at: 1606 1854 1606
Size: 248 3927
NcoI C'CATG_G
Cuts at: 398 398
Size: 4175
NgoMIV G'CCGG_C
Cuts at: 727 2354 727
Size: 1627 2548
NspI r_CATG'y
Cuts at: 1228 1728 2635 1228
Size: 500 907 2768
Fragments arranged by size:
2768 907 500
PciI A'CATG_T
Cuts at: 2631 2631
Size: 4175
PmeI GTTT'AAAC
Cuts at: 1174 1174
Size: 4175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 4143
PstI C_TGCA'G
Cuts at: 1084 1101 1222 1907 1084
Size: 17 121 685 3352
Fragments arranged by size:
3352 685 121 17
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4175
PvuII CAG'CTG
Cuts at: 687 1600 1960 687
Size: 913 360 2902
Fragments arranged by size:
2902 913 360
RsrII CG'GwC_CG
Cuts at: 2370 2370
Size: 4175
SacI G_AGCT'C
Cuts at: 1189 1189
Size: 4175
SalI G'TCGA_C
Cuts at: 1212 1212
Size: 4175
SapI GCTCTTCn'nnn_
Cuts at: 2198 2408 2198
Size: 210 3965
SbfI CC_TGCA'GG
Cuts at: 1222 1222
Size: 4175
ScaI AGT'ACT
Cuts at: 3692 3692
Size: 4175
SfcI C'TryA_G
Cuts at: 33 308 1080 1097 1218 1903 2896 3087 33
Size: 275 772 17 121 685 993 191 1121
Fragments arranged by size:
1121 993 772 685 275 191 121 17
SfoI GGC'GCC
Cuts at: 1607 1855 1607
Size: 248 3927
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
SmaI CCC'GGG
Cuts at: 1197 1197
Size: 4175
SmlI C'TyrA_G
Cuts at: 373 1330 2245 2737 2999 373
Size: 957 915 492 262 1549
Fragments arranged by size:
1549 957 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4175
SphI G_CATG'C
Cuts at: 1228 1728 1228
Size: 500 3675
StyI C'CwwG_G
Cuts at: 248 398 1309 1663 248
Size: 150 911 354 2760
Fragments arranged by size:
2760 911 354 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1758 1891 2350 2559 1758
Size: 133 459 209 3374
Fragments arranged by size:
3374 459 209 133
TatI w'GTAC_w
Cuts at: 3690 3690
Size: 4175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3692 3724 3692
Size: 32 4143
Tth111I GACn'n_nGTC
Cuts at: 1972 1972
Size: 4175
XbaI T'CTAG_A
Cuts at: 1206 1206
Size: 4175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 211 1670 211
Size: 1459 2716
XmaI C'CCGG_G
Cuts at: 1195 1195
Size: 4175
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbsI BbvCI BcgI BfrBI BmgBI BmtI
BpmI BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI