Restriction map for
pF1KB5237
(Circular) (Six-base) MAPSORT of: pF1KB5237.seq Check: 5233 from: 1 to: 3998
pF1KB5237 3998 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 302 1036 302
Size: 734 3264
Acc65I G'GTAC_C
Cuts at: 1014 1014
Size: 3998
AclI AA'CG_TT
Cuts at: 3838 3838
Size: 3998
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 1823 2255 3002 3270 188
Size: 1635 432 747 268 916
Fragments arranged by size:
1635 916 747 432 268
AflIII A'CryG_T
Cuts at: 1350 2454 3431 1350
Size: 1104 977 1917
Fragments arranged by size:
1917 1104 977
AgeI A'CCGG_T
Cuts at: 460 2386 460
Size: 1926 2072
AlwNI CAG_nnn'CTG
Cuts at: 1303 2870 1303
Size: 1567 2431
ApaLI G'TGCA_C
Cuts at: 2768 2768
Size: 3998
ApoI r'AATT_y
Cuts at: 850 1002 3351 3607 850
Size: 152 2349 256 1241
Fragments arranged by size:
2349 1241 256 152
AseI AT'TA_AT
Cuts at: 20 20
Size: 3998
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
AvaI C'yCGr_G
Cuts at: 128 142 1018 128
Size: 14 876 3108
Fragments arranged by size:
3108 876 14
BamHI G'GATC_C
Cuts at: 1023 1023
Size: 3998
BanI G'GyrC_C
Cuts at: 1014 1428 1676 1711 1014
Size: 414 248 35 3301
Fragments arranged by size:
3301 414 248 35
BanII G_rGCy'C
Cuts at: 1012 2042 1012
Size: 1030 2968
BbeI G_GCGC'C
Cuts at: 1432 1680 1432
Size: 248 3750
BbsI GAAGACnn'nnnn_
Cuts at: 174 420 174
Size: 246 3752
BciVI GTATCCnnnnn_n'
Cuts at: 1896 2657 3998 1896
Size: 761 1341 1896
Fragments arranged by size:
1896 1341 761
BclI T'GATC_A
Cuts at: 1518 1518
Size: 3998
BglI GCCn_nnn'nGGC
Cuts at: 193 193
Size: 3998
BglII A'GATC_T
Cuts at: 1513 3550 1513
Size: 2037 1961
BlpI GC'TnA_GC
Cuts at: 1110 1110
Size: 3998
Bme1580I G_kGCm'C
Cuts at: 1623 1716 2772 1623
Size: 93 1056 2849
Fragments arranged by size:
2849 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1210 1291 1626 3770 1210
Size: 81 335 2144 1438
Fragments arranged by size:
2144 1438 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 3998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 535
Size: 3998
Bpu10I CC'TnA_GC
Cuts at: 625 625
Size: 3998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1174 2053 2545 2843 1174
Size: 879 492 298 2329
Fragments arranged by size:
2329 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 106 139 3702 106
Size: 33 3563 402
Fragments arranged by size:
3563 402 33
BsaAI yAC'GTr
Cuts at: 1351 1351
Size: 3998
BsaBI GATnn'nnATC
Cuts at: 6 1512 3549 6
Size: 1506 2037 455
Fragments arranged by size:
2037 1506 455
BsaHI Gr'CG_yC
Cuts at: 1429 1677 2379 3875 1429
Size: 248 702 1496 1552
Fragments arranged by size:
1552 1496 702 248
BsaWI w'CCGG_w
Cuts at: 460 800 1187 1708 2386 2660 2807 3218 460
Size: 340 387 521 678 274 147 411 1240
Fragments arranged by size:
1240 678 521 411 387 340 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 466 496 869 899 466
Size: 30 373 30 3565
Fragments arranged by size:
3565 373 30 30
BseYI C'CCAG_C
Cuts at: 176 469 1423 2181 2758 3254 176
Size: 293 954 758 577 496 920
Fragments arranged by size:
954 920 758 577 496 293
BsiEI CG_ry'CG
Cuts at: 104 1540 1586 2794 104
Size: 1436 46 1208 1308
Fragments arranged by size:
1436 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 460 1012 1790 1980 2772 460
Size: 552 778 190 792 1686
Fragments arranged by size:
1686 792 778 552 190
Bsp1286I G_dGCh'C
Cuts at: 460 1012 1623 1716 1790 1980 2042 2772 460
Size: 552 611 93 74 190 62 730 1686
Fragments arranged by size:
1686 730 611 552 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3218 3218
Size: 3998
BspHI T'CATG_A
Cuts at: 3174 3174
Size: 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1048 1262 1564 1945 1048
Size: 214 302 381 3101
Fragments arranged by size:
3101 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2290 2344 3828 3993 50
Size: 2240 54 1484 165 55
Fragments arranged by size:
2240 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1910 1910
Size: 3998
BsrFI r'CCGG_y
Cuts at: 196 460 1996 2177 2386 196
Size: 264 1536 181 209 1808
Fragments arranged by size:
1808 1536 264 209 181
BssSI C'ACGA_G
Cuts at: 2269 2627 2269
Size: 358 3640
BstAPI GCAn_nnn'nTGC
Cuts at: 1375 1375
Size: 3998
BstBI TT'CG_AA
Cuts at: 2359 2359
Size: 3998
BstYI r'GATC_y
Cuts at: 1 1023 1490 1513 1848 2094 3106 3192 3204
Size: 1022 467 23 335 246 1012 86 12
Cuts at: 3204 3550 1
Size: 346 449
Fragments arranged by size:
1022 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3998
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1948 2129 2292 1948
Size: 181 163 3654
Fragments arranged by size:
3654 181 163
BtsI GCAGTG_nn'
Cuts at: 782 1241 782
Size: 459 3539
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 203 238 203
Size: 35 3963
DraI TTT'AAA
Cuts at: 997 997
Size: 3998
DraIII CAC_nnn'GTG
Cuts at: 371 371
Size: 3998
DrdI GACnn_nn'nnGTC
Cuts at: 1704 2562 1704
Size: 858 3140
EaeI y'GGCC_r
Cuts at: 194 1583 1757 2148 2175 3520 194
Size: 1389 174 391 27 1345 672
Fragments arranged by size:
1389 1345 672 391 174 27
EagI C'GGCC_G
Cuts at: 1583 1583
Size: 3998
EarI CTCTTCn'nnn_
Cuts at: 799 2021 2231 799
Size: 1222 210 2566
Fragments arranged by size:
2566 1222 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2206 2516 2662 3808 2206
Size: 310 146 1146 2396
Fragments arranged by size:
2396 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1010 1010
Size: 3998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 535 1823 2255 3002 3270 188
Size: 347 1288 432 747 268 916
Fragments arranged by size:
1288 916 747 432 347 268
EcoO109I rG'GnC_Cy
Cuts at: 132 1137 132
Size: 1005 2993
EcoRI G'AATT_C
Cuts at: 850 1002 850
Size: 152 3846
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2017 2017
Size: 3998
FspI TGC'GCA
Cuts at: 1779 1779
Size: 3998
HaeII r_GCGC'y
Cuts at: 203 1432 1680 2702 3681 203
Size: 1229 248 1022 979 520
Fragments arranged by size:
1229 1022 979 520 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1840 1870 1872 1902 1840
Size: 30 2 30 3936
Fragments arranged by size:
3936 30 30 2
HincII GTy'rAC
Cuts at: 798 1037 798
Size: 239 3759
HpaI GTT'AAC
Cuts at: 798 798
Size: 3998
KasI G'GCGC_C
Cuts at: 1428 1676 1428
Size: 248 3750
KpnI G_GTAC'C
Cuts at: 1018 1018
Size: 3998
MluI A'CGCG_T
Cuts at: 3431 3431
Size: 3998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2669 2853 2669
Size: 184 3814
MscI TGG'CCA
Cuts at: 1759 1759
Size: 3998
MslI CAynn'nnrTG
Cuts at: 1987 2114 1987
Size: 127 3871
MspA1I CmG'CkG
Cuts at: 1109 1423 1783 2796 3041 1109
Size: 314 360 1013 245 2066
Fragments arranged by size:
2066 1013 360 314 245
NaeI GCC'GGC
Cuts at: 198 2179 198
Size: 1981 2017
NarI GG'CG_CC
Cuts at: 1429 1677 1429
Size: 248 3750
NcoI C'CATG_G
Cuts at: 107 107
Size: 3998
NgoMIV G'CCGG_C
Cuts at: 196 2177 196
Size: 1981 2017
NspI r_CATG'y
Cuts at: 1051 1551 2458 1051
Size: 500 907 2591
Fragments arranged by size:
2591 907 500
PciI A'CATG_T
Cuts at: 2454 2454
Size: 3998
PfoI T'CCnGG_A
Cuts at: 632 632
Size: 3998
PmeI GTTT'AAAC
Cuts at: 997 997
Size: 3998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 447 479 3137 3169 447
Size: 32 2658 32 1276
Fragments arranged by size:
2658 1276 32 32
PpuMI rG'GwC_Cy
Cuts at: 132 132
Size: 3998
PshAI GACnn'nnGTC
Cuts at: 151 601 151
Size: 450 3548
PstI C_TGCA'G
Cuts at: 1045 1730 1045
Size: 685 3313
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3998
PvuII CAG'CTG
Cuts at: 1423 1783 1423
Size: 360 3638
RsrII CG'GwC_CG
Cuts at: 2193 2193
Size: 3998
SacI G_AGCT'C
Cuts at: 1012 1012
Size: 3998
SalI G'TCGA_C
Cuts at: 1035 1035
Size: 3998
SanDI GG'GwC_CC
Cuts at: 132 132
Size: 3998
SapI GCTCTTCn'nnn_
Cuts at: 2021 2231 2021
Size: 210 3788
SbfI CC_TGCA'GG
Cuts at: 1045 1045
Size: 3998
ScaI AGT'ACT
Cuts at: 3515 3515
Size: 3998
SfcI C'TryA_G
Cuts at: 33 1041 1726 2719 2910 33
Size: 1008 685 993 191 1121
Fragments arranged by size:
1121 1008 993 685 191
SfoI GGC'GCC
Cuts at: 1430 1678 1430
Size: 248 3750
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
SmaI CCC'GGG
Cuts at: 130 1020 130
Size: 890 3108
SmlI C'TyrA_G
Cuts at: 1153 2068 2560 2822 1153
Size: 915 492 262 2329
Fragments arranged by size:
2329 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3998
SphI G_CATG'C
Cuts at: 1051 1551 1051
Size: 500 3498
SspI AAT'ATT
Cuts at: 863 863
Size: 3998
StyI C'CwwG_G
Cuts at: 107 1132 1486 107
Size: 1025 354 2619
Fragments arranged by size:
2619 1025 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1581 1714 2173 2382 1581
Size: 133 459 209 3197
Fragments arranged by size:
3197 459 209 133
TatI w'GTAC_w
Cuts at: 808 3513 808
Size: 2705 1293
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 554 586 3515 3547 554
Size: 32 2929 32 1005
Fragments arranged by size:
2929 1005 32 32
Tth111I GACn'n_nGTC
Cuts at: 1795 1795
Size: 3998
XbaI T'CTAG_A
Cuts at: 1029 1029
Size: 3998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1493 1493
Size: 3998
XmaI C'CCGG_G
Cuts at: 128 1018 128
Size: 890 3108
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI EcoNI EcoRV FseI
FspAI HindIII MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI