Restriction map for
pF1KB5241
(Circular) (Six-base) MAPSORT of: pF1KB5241.seq Check: 7834 from: 1 to: 4547
pF1KB5241 4547 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AatII G_ACGT'C
Cuts at: 1295 1295
Size: 4547
AccI GT'mk_AC
Cuts at: 1445 1585 1445
Size: 140 4407
Acc65I G'GTAC_C
Cuts at: 1563 1563
Size: 4547
AclI AA'CG_TT
Cuts at: 4387 4387
Size: 4547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 577 772 802 830 1205 2372 2804 3551
Size: 234 195 30 28 375 1167 432 747
Cuts at: 3551 3819 343
Size: 268 1071
Fragments arranged by size:
1167 1071 747 432 375 268 234 195
30 28
AfeI AGC'GCT
Cuts at: 642 642
Size: 4547
AflIII A'CryG_T
Cuts at: 845 1899 3003 3980 845
Size: 1054 1104 977 1412
Fragments arranged by size:
1412 1104 1054 977
AgeI A'CCGG_T
Cuts at: 2935 2935
Size: 4547
AlwNI CAG_nnn'CTG
Cuts at: 1852 3419 1852
Size: 1567 2980
ApaI G_GGCC'C
Cuts at: 1497 1497
Size: 4547
ApaLI G'TGCA_C
Cuts at: 3317 3317
Size: 4547
ApoI r'AATT_y
Cuts at: 238 1191 1273 1551 3900 4156 238
Size: 953 82 278 2349 256 629
Fragments arranged by size:
2349 953 629 278 256 82
AseI AT'TA_AT
Cuts at: 20 20
Size: 4547
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
AvaI C'yCGr_G
Cuts at: 1567 1567
Size: 4547
BamHI G'GATC_C
Cuts at: 1572 1572
Size: 4547
BanI G'GyrC_C
Cuts at: 1563 1977 2225 2260 1563
Size: 414 248 35 3850
Fragments arranged by size:
3850 414 248 35
BanII G_rGCy'C
Cuts at: 1497 1561 2591 1497
Size: 64 1030 3453
Fragments arranged by size:
3453 1030 64
BbeI G_GCGC'C
Cuts at: 1981 2229 1981
Size: 248 4299
BciVI GTATCCnnnnn_n'
Cuts at: 2445 3206 4547 2445
Size: 761 1341 2445
Fragments arranged by size:
2445 1341 761
BclI T'GATC_A
Cuts at: 126 2067 126
Size: 1941 2606
BfrBI ATG'CAT
Cuts at: 1211 1211
Size: 4547
BglI GCCn_nnn'nGGC
Cuts at: 674 1041 674
Size: 367 4180
BglII A'GATC_T
Cuts at: 1413 2062 4099 1413
Size: 649 2037 1861
Fragments arranged by size:
2037 1861 649
BlpI GC'TnA_GC
Cuts at: 1659 1659
Size: 4547
Bme1580I G_kGCm'C
Cuts at: 1497 2172 2265 3321 1497
Size: 675 93 1056 2723
Fragments arranged by size:
2723 1056 675 93
BmrI ACTGGGnnnn_n'
Cuts at: 1759 1840 2175 4319 1759
Size: 81 335 2144 1987
Fragments arranged by size:
2144 1987 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2914 2914
Size: 4547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 1498 169
Size: 1329 3218
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 1446 1723 2602 3094 3392 940
Size: 506 277 879 492 298 2095
Fragments arranged by size:
2095 879 506 492 298 277
BsaI GGTCTCn'nnnn_
Cuts at: 4251 4251
Size: 4547
BsaAI yAC'GTr
Cuts at: 1900 1900
Size: 4547
BsaBI GATnn'nnATC
Cuts at: 6 128 1009 2061 4098 6
Size: 122 881 1052 2037 455
Fragments arranged by size:
2037 1052 881 455 122
BsaHI Gr'CG_yC
Cuts at: 1292 1978 2226 2928 4424 1292
Size: 686 248 702 1496 1415
Fragments arranged by size:
1496 1415 702 686 248
BsaWI w'CCGG_w
Cuts at: 1736 2257 2935 3209 3356 3767 1736
Size: 521 678 274 147 411 2516
Fragments arranged by size:
2516 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 399 399
Size: 4547
BseYI C'CCAG_C
Cuts at: 643 1972 2730 3307 3803 643
Size: 1329 758 577 496 1387
Fragments arranged by size:
1387 1329 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2089 2135 3343 104
Size: 1985 46 1208 1308
Fragments arranged by size:
1985 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1561 2339 2529 3321 1561
Size: 778 190 792 2787
Fragments arranged by size:
2787 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1497 1561 2172 2265 2339 2529 2591 3321 1497
Size: 64 611 93 74 190 62 730 2723
Fragments arranged by size:
2723 730 611 190 93 74 64 62
BspEI T'CCGG_A
Cuts at: 3767 3767
Size: 4547
BspHI T'CATG_A
Cuts at: 3723 3723
Size: 4547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1597 1811 2113 2494 1597
Size: 214 302 381 3650
Fragments arranged by size:
3650 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1491 2839 2893 4377 4542 50
Size: 1441 1348 54 1484 165 55
Fragments arranged by size:
1484 1441 1348 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2459 2459
Size: 4547
BsrFI r'CCGG_y
Cuts at: 2545 2726 2935 2545
Size: 181 209 4157
Fragments arranged by size:
4157 209 181
BssSI C'ACGA_G
Cuts at: 2818 3176 2818
Size: 358 4189
BstAPI GCAn_nnn'nTGC
Cuts at: 1924 1924
Size: 4547
BstBI TT'CG_AA
Cuts at: 2908 2908
Size: 4547
BstEII G'GTnAC_C
Cuts at: 393 393
Size: 4547
BstYI r'GATC_y
Cuts at: 1 1413 1572 2039 2062 2397 2643 3655 3741
Size: 1412 159 467 23 335 246 1012 86
Cuts at: 3741 3753 4099 1
Size: 12 346 449
Fragments arranged by size:
1412 1012 467 449 346 335 246 159
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 724 724
Size: 4547
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2497 2678 2841 2497
Size: 181 163 4203
Fragments arranged by size:
4203 181 163
BtsI GCAGTG_nn'
Cuts at: 1109 1790 1109
Size: 681 3866
DraI TTT'AAA
Cuts at: 681 1316 1546 681
Size: 635 230 3682
Fragments arranged by size:
3682 635 230
DrdI GACnn_nn'nnGTC
Cuts at: 2253 3111 2253
Size: 858 3689
EaeI y'GGCC_r
Cuts at: 2132 2306 2697 2724 4069 2132
Size: 174 391 27 1345 2610
Fragments arranged by size:
2610 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2132 2132
Size: 4547
EarI CTCTTCn'nnn_
Cuts at: 319 1181 2570 2780 319
Size: 862 1389 210 2086
Fragments arranged by size:
2086 1389 862 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2755 3065 3211 4357 2755
Size: 310 146 1146 2945
Fragments arranged by size:
2945 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1559 1559
Size: 4547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 343 577 772 802 830 1205 1498 2372
Size: 174 234 195 30 28 375 293 874
Cuts at: 2372 2804 3551 3819 169
Size: 432 747 268 897
Fragments arranged by size:
897 874 747 432 375 293 268 234
195 174 30 28
EcoNI CCTnn'n_nnAGG
Cuts at: 463 727 463
Size: 264 4283
EcoO109I rG'GnC_Cy
Cuts at: 1494 1537 1686 1494
Size: 43 149 4355
Fragments arranged by size:
4355 149 43
EcoRI G'AATT_C
Cuts at: 238 1551 238
Size: 1313 3234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 612 2566 612
Size: 1954 2593
FspI TGC'GCA
Cuts at: 2328 2328
Size: 4547
HaeII r_GCGC'y
Cuts at: 644 1981 2229 3251 4230 644
Size: 1337 248 1022 979 961
Fragments arranged by size:
1337 1022 979 961 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1521 1553 2389 2419 2421 2451 1521
Size: 32 836 30 2 30 3617
Fragments arranged by size:
3617 836 32 30 30 2
HincII GTy'rAC
Cuts at: 1586 1586
Size: 4547
HindIII A'AGCT_T
Cuts at: 487 784 1422 487
Size: 297 638 3612
Fragments arranged by size:
3612 638 297
KasI G'GCGC_C
Cuts at: 1977 2225 1977
Size: 248 4299
KpnI G_GTAC'C
Cuts at: 1567 1567
Size: 4547
MfeI C'AATT_G
Cuts at: 154 154
Size: 4547
MluI A'CGCG_T
Cuts at: 3980 3980
Size: 4547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3218 3402 3218
Size: 184 4363
MscI TGG'CCA
Cuts at: 2308 2308
Size: 4547
MslI CAynn'nnrTG
Cuts at: 2536 2663 2536
Size: 127 4420
MspA1I CmG'CkG
Cuts at: 1658 1972 2332 3345 3590 1658
Size: 314 360 1013 245 2615
Fragments arranged by size:
2615 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2728 2728
Size: 4547
NarI GG'CG_CC
Cuts at: 1978 2226 1978
Size: 248 4299
NgoMIV G'CCGG_C
Cuts at: 2726 2726
Size: 4547
NsiI A_TGCA'T
Cuts at: 1213 1213
Size: 4547
NspI r_CATG'y
Cuts at: 849 1600 2100 3007 849
Size: 751 500 907 2389
Fragments arranged by size:
2389 907 751 500
PciI A'CATG_T
Cuts at: 845 3003 845
Size: 2158 2389
PflMI CCAn_nnn'nTGG
Cuts at: 754 754
Size: 4547
PmeI GTTT'AAAC
Cuts at: 1546 1546
Size: 4547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4515
PpuMI rG'GwC_Cy
Cuts at: 1537 1537
Size: 4547
PshAI GACnn'nnGTC
Cuts at: 862 862
Size: 4547
PspOMI G'GGCC_C
Cuts at: 1493 1493
Size: 4547
PstI C_TGCA'G
Cuts at: 1104 1594 2279 1104
Size: 490 685 3372
Fragments arranged by size:
3372 685 490
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4547
PvuII CAG'CTG
Cuts at: 1972 2332 1972
Size: 360 4187
RsrII CG'GwC_CG
Cuts at: 2742 2742
Size: 4547
SacI G_AGCT'C
Cuts at: 1561 1561
Size: 4547
SalI G'TCGA_C
Cuts at: 1584 1584
Size: 4547
SapI GCTCTTCn'nnn_
Cuts at: 319 2570 2780 319
Size: 2251 210 2086
Fragments arranged by size:
2251 2086 210
SbfI CC_TGCA'GG
Cuts at: 1594 1594
Size: 4547
ScaI AGT'ACT
Cuts at: 1483 4064 1483
Size: 2581 1966
SfcI C'TryA_G
Cuts at: 33 507 1100 1590 2275 3268 3459 33
Size: 474 593 490 685 993 191 1121
Fragments arranged by size:
1121 993 685 593 490 474 191
SfoI GGC'GCC
Cuts at: 1979 2227 1979
Size: 248 4299
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
SmaI CCC'GGG
Cuts at: 1569 1569
Size: 4547
SmlI C'TyrA_G
Cuts at: 919 1425 1702 2617 3109 3371 919
Size: 506 277 915 492 262 2095
Fragments arranged by size:
2095 915 506 492 277 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4547
SphI G_CATG'C
Cuts at: 1600 2100 1600
Size: 500 4047
StuI AGG'CCT
Cuts at: 592 937 592
Size: 345 4202
StyI C'CwwG_G
Cuts at: 587 1434 1681 2035 587
Size: 847 247 354 3099
Fragments arranged by size:
3099 847 354 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 966 2130 2263 2722 2931 966
Size: 1164 133 459 209 2582
Fragments arranged by size:
2582 1164 459 209 133
TatI w'GTAC_w
Cuts at: 112 821 1481 4062 112
Size: 709 660 2581 597
Fragments arranged by size:
2581 709 660 597
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 989 1021 4064 4096 989
Size: 32 3043 32 1440
Fragments arranged by size:
3043 1440 32 32
Tth111I GACn'n_nGTC
Cuts at: 1535 2344 1535
Size: 809 3738
XbaI T'CTAG_A
Cuts at: 1137 1578 1137
Size: 441 4106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 258 1125 2042 258
Size: 867 917 2763
Fragments arranged by size:
2763 917 867
XmaI C'CCGG_G
Cuts at: 1567 1567
Size: 4547
ZraI GAC'GTC
Cuts at: 1293 1293
Size: 4547
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BmgBI BmtI Bpu10I BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I
BtgI ClaI CspCI DraIII EcoRV FseI FspAI HpaI
NcoI NdeI NheI NotI NruI PacI PasI PfoI
PmlI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI