Restriction map for
pF1KB5242
(Circular) (Six-base) MAPSORT of: pF1KB5242.seq Check: 8653 from: 1 to: 4679
pF1KB5242 4679 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1717 1717
Size: 4679
Acc65I G'GTAC_C
Cuts at: 1695 1695
Size: 4679
AclI AA'CG_TT
Cuts at: 4519 4519
Size: 4679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 999 1685 2504 2936 3289 3951 999
Size: 686 819 432 353 662 1727
Fragments arranged by size:
1727 819 686 662 432 353
AflIII A'CryG_T
Cuts at: 584 2031 3831 4112 584
Size: 1447 1800 281 1151
Fragments arranged by size:
1800 1447 1151 281
AgeI A'CCGG_T
Cuts at: 776 776
Size: 4679
AleI CACnn'nnGTG
Cuts at: 1117 1117
Size: 4679
AlwNI CAG_nnn'CTG
Cuts at: 349 765 798 1263 1290 1563 1644 1984 3422
Size: 416 33 465 27 273 81 340 1438
Cuts at: 3422 349
Size: 1606
Fragments arranged by size:
1606 1438 465 416 340 273 81 33
27
ApaI G_GGCC'C
Cuts at: 144 179 302 1524 144
Size: 35 123 1222 3299
Fragments arranged by size:
3299 1222 123 35
ApaLI G'TGCA_C
Cuts at: 3517 3517
Size: 4679
ApoI r'AATT_y
Cuts at: 1683 4032 4288 1683
Size: 2349 256 2074
Fragments arranged by size:
2349 2074 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4679
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
AvaI C'yCGr_G
Cuts at: 137 144 178 941 1436 1515 1699 137
Size: 7 34 763 495 79 184 3117
Fragments arranged by size:
3117 763 495 184 79 34 7
AvrII C'CTAG_G
Cuts at: 1539 1539
Size: 4679
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 847 880 1131 1164 847
Size: 33 251 33 4362
Fragments arranged by size:
4362 251 33 33
BamHI G'GATC_C
Cuts at: 932 1704 932
Size: 772 3907
BanI G'GyrC_C
Cuts at: 773 957 1004 1066 1535 1695 2109 2357 2392
Size: 184 47 62 469 160 414 248 35
Cuts at: 2392 773
Size: 3060
Fragments arranged by size:
3060 469 414 248 184 160 62 47
35
BanII G_rGCy'C
Cuts at: 144 179 302 391 791 1023 1133 1516 1524
Size: 35 123 89 400 232 110 383 8
Cuts at: 1524 1693 2723 144
Size: 169 1030 2100
Fragments arranged by size:
2100 1030 400 383 232 169 123 110
89 35 8
BbeI G_GCGC'C
Cuts at: 2113 2361 2113
Size: 248 4431
BbvCI CC'TCA_GC
Cuts at: 1244 1244
Size: 4679
BciVI GTATCCnnnnn_n'
Cuts at: 1132 2577 3633 4679 1132
Size: 1445 1056 1046 1132
Fragments arranged by size:
1445 1132 1056 1046
BclI T'GATC_A
Cuts at: 2199 2199
Size: 4679
BglI GCCn_nnn'nGGC
Cuts at: 879 1409 1496 1550 879
Size: 530 87 54 4008
Fragments arranged by size:
4008 530 87 54
BglII A'GATC_T
Cuts at: 2194 4231 2194
Size: 2037 2642
BlpI GC'TnA_GC
Cuts at: 1791 1791
Size: 4679
Bme1580I G_kGCm'C
Cuts at: 144 179 302 645 1178 1212 1524 2304 2397
Size: 35 123 343 533 34 312 780 93
Cuts at: 2397 3521 144
Size: 1124 1302
Fragments arranged by size:
1302 1124 780 533 343 312 123 93
35 34
BmrI ACTGGGnnnn_n'
Cuts at: 332 1891 1972 2307 4451 332
Size: 1559 81 335 2144 560
Fragments arranged by size:
2144 1559 560 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 241 3046 241
Size: 2805 1874
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 1147 1286 1606 1621 260
Size: 887 139 320 15 3318
Fragments arranged by size:
3318 887 320 139 15
Bpu10I CC'TnA_GC
Cuts at: 1244 1244
Size: 4679
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 946 1597 1855 2734 3448 3746 552
Size: 394 651 258 879 714 298 1485
Fragments arranged by size:
1485 879 714 651 394 298 258
BsaI GGTCTCn'nnnn_
Cuts at: 141 197 491 1433 4383 141
Size: 56 294 942 2950 437
Fragments arranged by size:
2950 942 437 294 56
BsaAI yAC'GTr
Cuts at: 585 741 2032 585
Size: 156 1291 3232
Fragments arranged by size:
3232 1291 156
BsaBI GATnn'nnATC
Cuts at: 6 2193 4230 6
Size: 2187 2037 455
Fragments arranged by size:
2187 2037 455
BsaHI Gr'CG_yC
Cuts at: 2110 2358 3060 4556 2110
Size: 248 702 1496 2233
Fragments arranged by size:
2233 1496 702 248
BsaWI w'CCGG_w
Cuts at: 776 1868 2389 3067 3478 3625 3899 776
Size: 1092 521 678 411 147 274 1556
Fragments arranged by size:
1556 1092 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 175 205 544 574 1284 1314 175
Size: 30 339 30 710 30 3540
Fragments arranged by size:
3540 710 339 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 344 1285 126
Size: 218 941 3520
Fragments arranged by size:
3520 941 218
BseYI C'CCAG_C
Cuts at: 301 411 424 758 791 1187 1263 1509 1607
Size: 110 13 334 33 396 76 246 98
Cuts at: 1607 1655 2104 2862 3527 3935 301
Size: 48 449 758 665 408 1045
Fragments arranged by size:
1045 758 665 449 408 396 334 246
110 98 76 48 33 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 577 1009 1296 362
Size: 215 432 287 3745
Fragments arranged by size:
3745 432 287 215
BsiEI CG_ry'CG
Cuts at: 104 1118 1206 1500 2221 2267 3497 104
Size: 1014 88 294 721 46 1230 1286
Fragments arranged by size:
1286 1230 1014 721 294 88 46
BsiHKAI G_wGCw'C
Cuts at: 752 1133 1693 2471 2661 3521 752
Size: 381 560 778 190 860 1910
Fragments arranged by size:
1910 860 778 560 381 190
Bsp1286I G_dGCh'C
Cuts at: 144 179 302 391 645 752 791 1023 1133
Size: 35 123 89 254 107 39 232 110
Cuts at: 1133 1178 1212 1516 1524 1693 2304 2397 2471
Size: 45 34 304 8 169 611 93 74
Cuts at: 2471 2661 2723 3521 144
Size: 190 62 798 1302
Fragments arranged by size:
1302 798 611 304 254 232 190 169
123 110 107 93 89 74 62 45
39 35 34 8
BspHI T'CATG_A
Cuts at: 3111 3111
Size: 4679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 175 334 504 902 1729 1943 2245 2626 175
Size: 159 170 398 827 214 302 381 2228
Fragments arranged by size:
2228 827 398 381 302 214 170 159
BsrBI CCG'CTC
Cuts at: 50 1533 2971 3025 4509 4674 50
Size: 1483 1438 54 1484 165 55
Fragments arranged by size:
1484 1483 1438 165 55 54
BsrDI GCAATG_nn'
Cuts at: 806 2591 806
Size: 1785 2894
BsrFI r'CCGG_y
Cuts at: 317 776 2677 2858 317
Size: 459 1901 181 2138
Fragments arranged by size:
2138 1901 459 181
BssHII G'CGCG_C
Cuts at: 435 435
Size: 4679
BssSI C'ACGA_G
Cuts at: 2950 3658 2950
Size: 708 3971
BstAPI GCAn_nnn'nTGC
Cuts at: 360 780 915 1173 2056 360
Size: 420 135 258 883 2983
Fragments arranged by size:
2983 883 420 258 135
BstBI TT'CG_AA
Cuts at: 3040 3040
Size: 4679
BstYI r'GATC_y
Cuts at: 1 932 1704 2171 2194 2529 2775 3081 3093
Size: 931 772 467 23 335 246 306 12
Cuts at: 3093 3179 4231 1
Size: 86 1052 449
Fragments arranged by size:
1052 931 772 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1525 1624 1525
Size: 99 4580
BtgI C'CryG_G
Cuts at: 118 493 1118 118
Size: 375 625 3679
Fragments arranged by size:
3679 625 375
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2629 2810 2973 2629
Size: 181 163 4335
Fragments arranged by size:
4335 181 163
BtsI GCAGTG_nn'
Cuts at: 520 1922 520
Size: 1402 3277
DraI TTT'AAA
Cuts at: 1678 1678
Size: 4679
DrdI GACnn_nn'nnGTC
Cuts at: 2385 3729 2385
Size: 1344 3335
EaeI y'GGCC_r
Cuts at: 490 1115 1497 2264 2438 2829 2856 4201 490
Size: 625 382 767 174 391 27 1345 968
Fragments arranged by size:
1345 968 767 625 391 382 174 27
EagI C'GGCC_G
Cuts at: 1115 1497 2264 1115
Size: 382 767 3530
Fragments arranged by size:
3530 767 382
EarI CTCTTCn'nnn_
Cuts at: 379 2702 2912 379
Size: 2323 210 2146
Fragments arranged by size:
2323 2146 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 655 2887 3629 3775 4489 655
Size: 2232 742 146 714 845
Fragments arranged by size:
2232 845 742 714 146
EcoICRI GAG'CTC
Cuts at: 1131 1691 1131
Size: 560 4119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 999 1147 1286 1606 1621 1685 2504 2936
Size: 739 148 139 320 15 64 819 432
Cuts at: 2936 3289 3951 260
Size: 353 662 988
Fragments arranged by size:
988 819 739 662 432 353 320 148
139 64 15
EcoNI CCTnn'n_nnAGG
Cuts at: 326 353 649 326
Size: 27 296 4356
Fragments arranged by size:
4356 296 27
EcoO109I rG'GnC_Cy
Cuts at: 141 175 298 334 346 1147 1287 1364 1371
Size: 34 123 36 12 801 140 77 7
Cuts at: 1371 1397 1520 1521 1641 1660 1818 141
Size: 26 123 1 120 19 158 3002
Fragments arranged by size:
3002 801 158 140 123 123 120 77
36 34 26 19 12 7 1
EcoRI G'AATT_C
Cuts at: 1683 1683
Size: 4679
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 4679
FspI TGC'GCA
Cuts at: 171 2460 171
Size: 2289 2390
HaeII r_GCGC'y
Cuts at: 460 1430 2113 2361 3591 4362 460
Size: 970 683 248 1230 771 777
Fragments arranged by size:
1230 970 777 771 683 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 175 207 924 956 1074 1106 1284 1316 2521
Size: 32 717 32 118 32 178 32 1205
Cuts at: 2521 2551 2553 2583 175
Size: 30 2 30 2271
Fragments arranged by size:
2271 1205 717 178 118 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 975 1718 975
Size: 743 3936
KasI G'GCGC_C
Cuts at: 2109 2357 2109
Size: 248 4431
KpnI G_GTAC'C
Cuts at: 1699 1699
Size: 4679
MluI A'CGCG_T
Cuts at: 4112 4112
Size: 4679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 306 3438 3622 306
Size: 3132 184 1363
Fragments arranged by size:
3132 1363 184
MscI TGG'CCA
Cuts at: 2440 2440
Size: 4679
MslI CAynn'nnrTG
Cuts at: 583 982 1117 2668 2795 583
Size: 399 135 1551 127 2467
Fragments arranged by size:
2467 1551 399 135 127
MspA1I CmG'CkG
Cuts at: 120 366 1214 1260 1790 2104 2464 3248 3493
Size: 246 848 46 530 314 360 784 245
Cuts at: 3493 120
Size: 1306
Fragments arranged by size:
1306 848 784 530 360 314 246 245
46
NaeI GCC'GGC
Cuts at: 2860 2860
Size: 4679
NarI GG'CG_CC
Cuts at: 2110 2358 2110
Size: 248 4431
NgoMIV G'CCGG_C
Cuts at: 2858 2858
Size: 4679
NspI r_CATG'y
Cuts at: 1732 2232 3835 1732
Size: 500 1603 2576
Fragments arranged by size:
2576 1603 500
PasI CC'CwG_GG
Cuts at: 391 1644 391
Size: 1253 3426
PciI A'CATG_T
Cuts at: 3831 3831
Size: 4679
PflMI CCAn_nnn'nTGG
Cuts at: 308 480 625 744 765 798 819 1263 1455
Size: 172 145 119 21 33 21 444 192
Cuts at: 1455 1644 308
Size: 189 3343
Fragments arranged by size:
3343 444 192 189 172 145 119 33
21 21
PmeI GTTT'AAAC
Cuts at: 1678 1678
Size: 4679
PmlI CAC'GTG
Cuts at: 585 741 585
Size: 156 4523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3125 3157 3125
Size: 32 4647
PpuMI rG'GwC_Cy
Cuts at: 346 1147 1287 1364 1371 1397 346
Size: 801 140 77 7 26 3628
Fragments arranged by size:
3628 801 140 77 26 7
PshAI GACnn'nnGTC
Cuts at: 533 533
Size: 4679
PspOMI G'GGCC_C
Cuts at: 140 175 298 1520 140
Size: 35 123 1222 3299
Fragments arranged by size:
3299 1222 123 35
PspXI vC'TCGA_Gb
Cuts at: 1515 1515
Size: 4679
PstI C_TGCA'G
Cuts at: 365 527 1726 2411 365
Size: 162 1199 685 2633
Fragments arranged by size:
2633 1199 685 162
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4679
PvuII CAG'CTG
Cuts at: 1260 2104 2464 1260
Size: 844 360 3475
Fragments arranged by size:
3475 844 360
RsrII CG'GwC_CG
Cuts at: 2874 2874
Size: 4679
SacI G_AGCT'C
Cuts at: 1133 1693 1133
Size: 560 4119
SacII CC_GC'GG
Cuts at: 121 121
Size: 4679
SalI G'TCGA_C
Cuts at: 1716 1716
Size: 4679
SanDI GG'GwC_CC
Cuts at: 1147 1147
Size: 4679
SapI GCTCTTCn'nnn_
Cuts at: 379 2702 2912 379
Size: 2323 210 2146
Fragments arranged by size:
2323 2146 210
SbfI CC_TGCA'GG
Cuts at: 1726 1726
Size: 4679
ScaI AGT'ACT
Cuts at: 4196 4196
Size: 4679
SfcI C'TryA_G
Cuts at: 33 361 523 968 1722 2407 3375 3566 33
Size: 328 162 445 754 685 968 191 1146
Fragments arranged by size:
1146 968 754 685 445 328 191 162
SfoI GGC'GCC
Cuts at: 2111 2359 2111
Size: 248 4431
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
SmaI CCC'GGG
Cuts at: 139 180 1701 139
Size: 41 1521 3117
Fragments arranged by size:
3117 1521 41
SmlI C'TyrA_G
Cuts at: 531 925 1436 1515 1612 1834 2749 3463 3725
Size: 394 511 79 97 222 915 714 262
Cuts at: 3725 531
Size: 1485
Fragments arranged by size:
1485 915 714 511 394 262 222 97
79
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4679
SphI G_CATG'C
Cuts at: 1732 2232 1732
Size: 500 4179
StuI AGG'CCT
Cuts at: 1544 1601 1544
Size: 57 4622
StyI C'CwwG_G
Cuts at: 1393 1539 1813 2167 1393
Size: 146 274 354 3905
Fragments arranged by size:
3905 354 274 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 165 216 2262 2395 2854 3063 165
Size: 51 2046 133 459 209 1781
Fragments arranged by size:
2046 1781 459 209 133 51
TatI w'GTAC_w
Cuts at: 279 1220 4194 279
Size: 941 2974 764
Fragments arranged by size:
2974 941 764
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4196 4228 4196
Size: 32 4647
Tth111I GACn'n_nGTC
Cuts at: 2476 2476
Size: 4679
XbaI T'CTAG_A
Cuts at: 1710 1710
Size: 4679
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2174 2174
Size: 4679
XhoI C'TCGA_G
Cuts at: 1436 1515 1436
Size: 79 4600
XmaI C'CCGG_G
Cuts at: 137 178 1699 137
Size: 41 1521 3117
Fragments arranged by size:
3117 1521 41
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
BbsI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BspEI BsrGI BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PfoI PsiI PsrI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI
ZraI