Restriction map for
pF1KB5244
(Circular) (Six-base) MAPSORT of: pF1KB5244.seq Check: 1171 from: 1 to: 4181
pF1KB5244 4181 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 734 734
Size: 4181
AccI GT'mk_AC
Cuts at: 1219 1219
Size: 4181
Acc65I G'GTAC_C
Cuts at: 802 1197 802
Size: 395 3786
AclI AA'CG_TT
Cuts at: 4021 4021
Size: 4181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2006 2438 2791 3453 2006
Size: 432 353 662 2734
Fragments arranged by size:
2734 662 432 353
AfeI AGC'GCT
Cuts at: 741 863 741
Size: 122 4059
AflIII A'CryG_T
Cuts at: 1533 3333 3614 1533
Size: 1800 281 2100
Fragments arranged by size:
2100 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 505 1034 505
Size: 529 3652
AlwNI CAG_nnn'CTG
Cuts at: 516 1486 2924 516
Size: 970 1438 1773
Fragments arranged by size:
1773 1438 970
ApaI G_GGCC'C
Cuts at: 1041 1041
Size: 4181
ApaLI G'TGCA_C
Cuts at: 3019 3019
Size: 4181
ApoI r'AATT_y
Cuts at: 1185 3534 3790 1185
Size: 2349 256 1576
Fragments arranged by size:
2349 1576 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4181
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
AvaI C'yCGr_G
Cuts at: 431 1201 431
Size: 770 3411
BamHI G'GATC_C
Cuts at: 1206 1206
Size: 4181
BanI G'GyrC_C
Cuts at: 489 802 1066 1197 1611 1859 1894 489
Size: 313 264 131 414 248 35 2776
Fragments arranged by size:
2776 414 313 264 248 131 35
BanII G_rGCy'C
Cuts at: 564 872 1041 1195 2225 564
Size: 308 169 154 1030 2520
Fragments arranged by size:
2520 1030 308 169 154
BbeI G_GCGC'C
Cuts at: 1615 1863 1615
Size: 248 3933
BbsI GAAGACnn'nnnn_
Cuts at: 816 816
Size: 4181
BbvCI CC'TCA_GC
Cuts at: 149 925 149
Size: 776 3405
BciVI GTATCCnnnnn_n'
Cuts at: 2079 3135 4181 2079
Size: 1056 1046 2079
Fragments arranged by size:
2079 1056 1046
BclI T'GATC_A
Cuts at: 1701 1701
Size: 4181
BglII A'GATC_T
Cuts at: 1696 3733 1696
Size: 2037 2144
BlpI GC'TnA_GC
Cuts at: 937 1293 937
Size: 356 3825
Bme1580I G_kGCm'C
Cuts at: 1041 1152 1806 1899 3023 1041
Size: 111 654 93 1124 2199
Fragments arranged by size:
2199 1124 654 111 93
BmrI ACTGGGnnnn_n'
Cuts at: 782 1393 1474 1809 3953 782
Size: 611 81 335 2144 1010
Fragments arranged by size:
2144 1010 611 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 276 2548 276
Size: 2272 1909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 886 415
Size: 471 3710
Bpu10I CC'TnA_GC
Cuts at: 149 582 698 925 149
Size: 433 116 227 3405
Fragments arranged by size:
3405 433 227 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1357 2236 2950 3248 1357
Size: 879 714 298 2290
Fragments arranged by size:
2290 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 609 3885 609
Size: 3276 905
BsaAI yAC'GTr
Cuts at: 1534 1534
Size: 4181
BsaBI GATnn'nnATC
Cuts at: 6 338 1695 3732 6
Size: 332 1357 2037 455
Fragments arranged by size:
2037 1357 455 332
BsaHI Gr'CG_yC
Cuts at: 1612 1860 2562 4058 1612
Size: 248 702 1496 1735
Fragments arranged by size:
1735 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1370 1891 2569 2980 3127 3401 1370
Size: 521 678 411 147 274 2150
Fragments arranged by size:
2150 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 589 619 748 778 589
Size: 30 129 30 3992
Fragments arranged by size:
3992 129 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 268 295 298 325 343 217
Size: 51 27 3 27 18 4055
Fragments arranged by size:
4055 51 27 27 18 3
BseYI C'CCAG_C
Cuts at: 1151 1606 2364 3029 3437 1151
Size: 455 758 665 408 1895
Fragments arranged by size:
1895 758 665 455 408
BsiEI CG_ry'CG
Cuts at: 104 1723 1769 2999 104
Size: 1619 46 1230 1286
Fragments arranged by size:
1619 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 209 1195 1973 2163 3023 209
Size: 986 778 190 860 1367
Fragments arranged by size:
1367 986 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 348 405 348
Size: 57 4124
Bsp1286I G_dGCh'C
Cuts at: 209 564 872 1041 1152 1195 1806 1899 1973
Size: 355 308 169 111 43 611 93 74
Cuts at: 1973 2163 2225 3023 209
Size: 190 62 798 1367
Fragments arranged by size:
1367 798 611 355 308 190 169 111
93 74 62 43
BspHI T'CATG_A
Cuts at: 1131 1142 1170 2613 1131
Size: 11 28 1443 2699
Fragments arranged by size:
2699 1443 28 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 485 734 1231 1445 1747 2128 485
Size: 249 497 214 302 381 2538
Fragments arranged by size:
2538 497 381 302 249 214
BsrBI CCG'CTC
Cuts at: 50 438 936 2473 2527 4011 4176 50
Size: 388 498 1537 54 1484 165 55
Fragments arranged by size:
1537 1484 498 388 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2093 2093
Size: 4181
BsrFI r'CCGG_y
Cuts at: 2179 2360 2179
Size: 181 4000
BsrGI T'GTAC_A
Cuts at: 753 753
Size: 4181
BssSI C'ACGA_G
Cuts at: 208 2452 3160 208
Size: 2244 708 1229
Fragments arranged by size:
2244 1229 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1558 1558
Size: 4181
BstBI TT'CG_AA
Cuts at: 2542 2542
Size: 4181
BstXI CCAn_nnnn'nTGG
Cuts at: 654 778 654
Size: 124 4057
BstYI r'GATC_y
Cuts at: 1 572 1088 1206 1673 1696 2031 2277 2583
Size: 571 516 118 467 23 335 246 306
Cuts at: 2583 2595 2681 3733 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 571 516 467 449 335 306 246
118 86 23 12
BtgI C'CryG_G
Cuts at: 806 1047 806
Size: 241 3940
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2131 2312 2475 2131
Size: 181 163 3837
Fragments arranged by size:
3837 181 163
BtsI GCAGTG_nn'
Cuts at: 1424 1424
Size: 4181
DraI TTT'AAA
Cuts at: 405 1180 405
Size: 775 3406
DrdI GACnn_nn'nnGTC
Cuts at: 1887 3231 1887
Size: 1344 2837
EaeI y'GGCC_r
Cuts at: 542 734 1766 1940 2331 2358 3703 542
Size: 192 1032 174 391 27 1345 1020
Fragments arranged by size:
1345 1032 1020 391 192 174 27
EagI C'GGCC_G
Cuts at: 1766 1766
Size: 4181
EarI CTCTTCn'nnn_
Cuts at: 302 314 320 2204 2414 302
Size: 12 6 1884 210 2069
Fragments arranged by size:
2069 1884 210 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 782 830 966 2389 3131 3277 3991 782
Size: 48 136 1423 742 146 714 972
Fragments arranged by size:
1423 972 742 714 146 136 48
EcoICRI GAG'CTC
Cuts at: 1193 1193
Size: 4181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 886 2006 2438 2791 3453 415
Size: 471 1120 432 353 662 1143
Fragments arranged by size:
1143 1120 662 471 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 307 481 307
Size: 174 4007
EcoO109I rG'GnC_Cy
Cuts at: 160 300 1037 1320 160
Size: 140 737 283 3021
Fragments arranged by size:
3021 737 283 140
EcoRI G'AATT_C
Cuts at: 1185 1185
Size: 4181
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 4181
FspI TGC'GCA
Cuts at: 481 1962 481
Size: 1481 2700
HaeII r_GCGC'y
Cuts at: 469 743 865 1615 1863 3093 3864 469
Size: 274 122 750 248 1230 771 786
Fragments arranged by size:
1230 786 771 750 274 248 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 372 404 589 621 2023 2053 2055 2085 372
Size: 32 185 32 1402 30 2 30 2468
Fragments arranged by size:
2468 1402 185 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1220 1220
Size: 4181
KasI G'GCGC_C
Cuts at: 1611 1859 1611
Size: 248 3933
KpnI G_GTAC'C
Cuts at: 806 1201 806
Size: 395 3786
MluI A'CGCG_T
Cuts at: 3614 3614
Size: 4181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 548 2940 3124 548
Size: 2392 184 1605
Fragments arranged by size:
2392 1605 184
MscI TGG'CCA
Cuts at: 544 1942 544
Size: 1398 2783
MslI CAynn'nnrTG
Cuts at: 2170 2297 2170
Size: 127 4054
MspA1I CmG'CkG
Cuts at: 1072 1292 1606 1966 2750 2995 1072
Size: 220 314 360 784 245 2258
Fragments arranged by size:
2258 784 360 314 245 220
NaeI GCC'GGC
Cuts at: 2362 2362
Size: 4181
NarI GG'CG_CC
Cuts at: 1612 1860 1612
Size: 248 3933
NcoI C'CATG_G
Cuts at: 806 806
Size: 4181
NgoMIV G'CCGG_C
Cuts at: 2360 2360
Size: 4181
NspI r_CATG'y
Cuts at: 450 717 1234 1734 3337 450
Size: 267 517 500 1603 1294
Fragments arranged by size:
1603 1294 517 500 267
PciI A'CATG_T
Cuts at: 3333 3333
Size: 4181
PmeI GTTT'AAAC
Cuts at: 1180 1180
Size: 4181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2627 2659 2627
Size: 32 4149
PpuMI rG'GwC_Cy
Cuts at: 160 300 160
Size: 140 4041
PshAI GACnn'nnGTC
Cuts at: 182 182
Size: 4181
PspOMI G'GGCC_C
Cuts at: 1037 1037
Size: 4181
PstI C_TGCA'G
Cuts at: 1023 1228 1913 1023
Size: 205 685 3291
Fragments arranged by size:
3291 685 205
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4181
PvuII CAG'CTG
Cuts at: 1606 1966 1606
Size: 360 3821
RsrII CG'GwC_CG
Cuts at: 2376 2376
Size: 4181
SacI G_AGCT'C
Cuts at: 1195 1195
Size: 4181
SalI G'TCGA_C
Cuts at: 1218 1218
Size: 4181
SapI GCTCTTCn'nnn_
Cuts at: 2204 2414 2204
Size: 210 3971
SbfI CC_TGCA'GG
Cuts at: 1228 1228
Size: 4181
ScaI AGT'ACT
Cuts at: 3698 3698
Size: 4181
SexAI A'CCwGG_T
Cuts at: 599 653 599
Size: 54 4127
SfcI C'TryA_G
Cuts at: 33 852 1019 1224 1909 2877 3068 33
Size: 819 167 205 685 968 191 1146
Fragments arranged by size:
1146 968 819 685 205 191 167
SfoI GGC'GCC
Cuts at: 1613 1861 1613
Size: 248 3933
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
SmaI CCC'GGG
Cuts at: 433 1203 433
Size: 770 3411
SmlI C'TyrA_G
Cuts at: 1336 2251 2965 3227 1336
Size: 915 714 262 2290
Fragments arranged by size:
2290 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4181
SphI G_CATG'C
Cuts at: 450 1234 1734 450
Size: 784 500 2897
Fragments arranged by size:
2897 784 500
SspI AAT'ATT
Cuts at: 1104 1104
Size: 4181
StyI C'CwwG_G
Cuts at: 156 806 1315 1669 156
Size: 650 509 354 2668
Fragments arranged by size:
2668 650 509 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1764 1897 2356 2565 1764
Size: 133 459 209 3380
Fragments arranged by size:
3380 459 209 133
TatI w'GTAC_w
Cuts at: 753 3696 753
Size: 2943 1238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 748 780 958 990 3698 3730 748
Size: 32 178 32 2708 32 1199
Fragments arranged by size:
2708 1199 178 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 601 1978 601
Size: 1377 2804
XbaI T'CTAG_A
Cuts at: 1212 1212
Size: 4181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 651 1676 651
Size: 1025 3156
XmaI C'CCGG_G
Cuts at: 431 1201 431
Size: 770 3411
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AloI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI
BsmI BspEI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI