Restriction map for
pF1KB5260
(Circular) (Six-base) MAPSORT of: pF1KB5260.seq Check: 1939 from: 1 to: 4256
pF1KB5260 4256 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1294 1294
Size: 4256
Acc65I G'GTAC_C
Cuts at: 1272 1272
Size: 4256
AclI AA'CG_TT
Cuts at: 4096 4096
Size: 4256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 2081 2513 3260 3528 172
Size: 1909 432 747 268 900
Fragments arranged by size:
1909 900 747 432 268
AflIII A'CryG_T
Cuts at: 1608 2712 3689 1608
Size: 1104 977 2175
Fragments arranged by size:
2175 1104 977
AgeI A'CCGG_T
Cuts at: 2644 2644
Size: 4256
AhdI GACnn_n'nnGTC
Cuts at: 438 438
Size: 4256
AleI CACnn'nnGTG
Cuts at: 1123 1123
Size: 4256
AlwNI CAG_nnn'CTG
Cuts at: 1561 3128 1561
Size: 1567 2689
ApaI G_GGCC'C
Cuts at: 1139 1190 1139
Size: 51 4205
ApaLI G'TGCA_C
Cuts at: 3026 3026
Size: 4256
ApoI r'AATT_y
Cuts at: 1260 3609 3865 1260
Size: 2349 256 1651
Fragments arranged by size:
2349 1651 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4256
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
AvaI C'yCGr_G
Cuts at: 1109 1130 1276 1109
Size: 21 146 4089
Fragments arranged by size:
4089 146 21
BamHI G'GATC_C
Cuts at: 185 1281 185
Size: 1096 3160
BanI G'GyrC_C
Cuts at: 730 1000 1055 1272 1686 1934 1969 730
Size: 270 55 217 414 248 35 3017
Fragments arranged by size:
3017 414 270 248 217 55 35
BanII G_rGCy'C
Cuts at: 926 1139 1190 1270 2300 926
Size: 213 51 80 1030 2882
Fragments arranged by size:
2882 1030 213 80 51
BbeI G_GCGC'C
Cuts at: 1059 1690 1938 1059
Size: 631 248 3377
Fragments arranged by size:
3377 631 248
BbvCI CC'TCA_GC
Cuts at: 826 856 826
Size: 30 4226
BciVI GTATCCnnnnn_n'
Cuts at: 2154 2915 4256 2154
Size: 761 1341 2154
Fragments arranged by size:
2154 1341 761
BclI T'GATC_A
Cuts at: 639 1776 639
Size: 1137 3119
BglI GCCn_nnn'nGGC
Cuts at: 155 155
Size: 4256
BglII A'GATC_T
Cuts at: 1771 3808 1771
Size: 2037 2219
BlpI GC'TnA_GC
Cuts at: 1368 1368
Size: 4256
Bme1580I G_kGCm'C
Cuts at: 1003 1139 1190 1881 1974 3030 1003
Size: 136 51 691 93 1056 2229
Fragments arranged by size:
2229 1056 691 136 93 51
BmrI ACTGGGnnnn_n'
Cuts at: 1468 1549 1884 4028 1468
Size: 81 335 2144 1696
Fragments arranged by size:
2144 1696 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 4256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 904 940 298
Size: 606 36 3614
Fragments arranged by size:
3614 606 36
Bpu10I CC'TnA_GC
Cuts at: 826 856 826
Size: 30 4226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1126 1189 1432 2311 2803 3101 754
Size: 372 63 243 879 492 298 1909
Fragments arranged by size:
1909 879 492 372 298 243 63
BsaI GGTCTCn'nnnn_
Cuts at: 870 3960 870
Size: 3090 1166
BsaAI yAC'GTr
Cuts at: 171 663 1044 1609 171
Size: 492 381 565 2818
Fragments arranged by size:
2818 565 492 381
BsaBI GATnn'nnATC
Cuts at: 6 1770 3807 6
Size: 1764 2037 455
Fragments arranged by size:
2037 1764 455
BsaHI Gr'CG_yC
Cuts at: 434 1056 1687 1935 2637 4133 434
Size: 622 631 248 702 1496 557
Fragments arranged by size:
1496 702 631 622 557 248
BsaWI w'CCGG_w
Cuts at: 1445 1966 2644 2918 3065 3476 1445
Size: 521 678 274 147 411 2225
Fragments arranged by size:
2225 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 662 692 662
Size: 30 4226
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 361 413 442 558 862 1051 151
Size: 210 52 29 116 304 189 3356
Fragments arranged by size:
3356 304 210 189 116 52 29
BseYI C'CCAG_C
Cuts at: 586 1182 1681 2439 3016 3512 586
Size: 596 499 758 577 496 1330
Fragments arranged by size:
1330 758 596 577 499 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 193 163
Size: 30 4226
BsiEI CG_ry'CG
Cuts at: 104 749 789 1798 1844 3052 104
Size: 645 40 1009 46 1208 1308
Fragments arranged by size:
1308 1208 1009 645 46 40
BsiHKAI G_wGCw'C
Cuts at: 266 518 1270 2048 2238 3030 266
Size: 252 752 778 190 792 1492
Fragments arranged by size:
1492 792 778 752 252 190
Bsp1286I G_dGCh'C
Cuts at: 266 518 926 1003 1139 1190 1270 1881 1974
Size: 252 408 77 136 51 80 611 93
Cuts at: 1974 2048 2238 2300 3030 266
Size: 74 190 62 730 1492
Fragments arranged by size:
1492 730 611 408 252 190 136 93
80 77 74 62 51
BspEI T'CCGG_A
Cuts at: 3476 3476
Size: 4256
BspHI T'CATG_A
Cuts at: 3432 3432
Size: 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 183 1306 1520 1822 2203 183
Size: 1123 214 302 381 2236
Fragments arranged by size:
2236 1123 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2548 2602 4086 4251 50
Size: 2498 54 1484 165 55
Fragments arranged by size:
2498 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2168 2168
Size: 4256
BsrFI r'CCGG_y
Cuts at: 392 800 1022 1103 1160 2254 2435 2644 392
Size: 408 222 81 57 1094 181 209 2004
Fragments arranged by size:
2004 1094 408 222 209 181 81 57
BsrGI T'GTAC_A
Cuts at: 419 419
Size: 4256
BssSI C'ACGA_G
Cuts at: 2527 2885 2527
Size: 358 3898
BstAPI GCAn_nnn'nTGC
Cuts at: 1633 1633
Size: 4256
BstBI TT'CG_AA
Cuts at: 2617 2617
Size: 4256
BstEII G'GTnAC_C
Cuts at: 678 678
Size: 4256
BstXI CCAn_nnnn'nTGG
Cuts at: 1125 1125
Size: 4256
BstYI r'GATC_y
Cuts at: 1 185 1199 1281 1748 1771 2106 2352 3364
Size: 184 1014 82 467 23 335 246 1012
Cuts at: 3364 3450 3462 3808 1
Size: 86 12 346 449
Fragments arranged by size:
1014 1012 467 449 346 335 246 184
86 82 23 12
BtgI C'CryG_G
Cuts at: 107 905 107
Size: 798 3458
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 567 2206 2387 2550 567
Size: 1639 181 163 2273
Fragments arranged by size:
2273 1639 181 163
BtsI GCAGTG_nn'
Cuts at: 1090 1499 1090
Size: 409 3847
DraI TTT'AAA
Cuts at: 1255 1255
Size: 4256
DrdI GACnn_nn'nnGTC
Cuts at: 211 1962 2820 211
Size: 1751 858 1647
Fragments arranged by size:
1751 1647 858
EaeI y'GGCC_r
Cuts at: 491 1115 1841 2015 2406 2433 3778 491
Size: 624 726 174 391 27 1345 969
Fragments arranged by size:
1345 969 726 624 391 174 27
EagI C'GGCC_G
Cuts at: 1841 1841
Size: 4256
EarI CTCTTCn'nnn_
Cuts at: 523 2279 2489 523
Size: 1756 210 2290
Fragments arranged by size:
2290 1756 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 925 2464 2774 2920 4066 925
Size: 1539 310 146 1146 1115
Fragments arranged by size:
1539 1146 1115 310 146
EcoICRI GAG'CTC
Cuts at: 1268 1268
Size: 4256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 298 904 940 2081 2513 3260 3528 172
Size: 126 606 36 1141 432 747 268 900
Fragments arranged by size:
1141 900 747 606 432 268 126 36
EcoNI CCTnn'n_nnAGG
Cuts at: 447 859 447
Size: 412 3844
EcoO109I rG'GnC_Cy
Cuts at: 195 1063 1135 1136 1186 1187 1395 195
Size: 868 72 1 50 1 208 3056
Fragments arranged by size:
3056 868 208 72 50 1 1
EcoRI G'AATT_C
Cuts at: 1260 1260
Size: 4256
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2275 2275
Size: 4256
FspI TGC'GCA
Cuts at: 2037 2037
Size: 4256
HaeII r_GCGC'y
Cuts at: 721 1023 1059 1690 1938 2960 3939 721
Size: 302 36 631 248 1022 979 1038
Fragments arranged by size:
1038 1022 979 631 302 248 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 337 369 424 456 2098 2128 2130 2160 337
Size: 32 55 32 1642 30 2 30 2433
Fragments arranged by size:
2433 1642 55 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1295 1295
Size: 4256
KasI G'GCGC_C
Cuts at: 1055 1686 1934 1055
Size: 631 248 3377
Fragments arranged by size:
3377 631 248
KpnI G_GTAC'C
Cuts at: 1276 1276
Size: 4256
MluI A'CGCG_T
Cuts at: 3689 3689
Size: 4256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 311 2927 3111 311
Size: 2616 184 1456
Fragments arranged by size:
2616 1456 184
MscI TGG'CCA
Cuts at: 493 2017 493
Size: 1524 2732
MslI CAynn'nnrTG
Cuts at: 530 811 1123 2245 2372 530
Size: 281 312 1122 127 2414
Fragments arranged by size:
2414 1122 312 281 127
MspA1I CmG'CkG
Cuts at: 177 1367 1681 2041 3054 3299 177
Size: 1190 314 360 1013 245 1134
Fragments arranged by size:
1190 1134 1013 360 314 245
NaeI GCC'GGC
Cuts at: 394 2437 394
Size: 2043 2213
NarI GG'CG_CC
Cuts at: 1056 1687 1935 1056
Size: 631 248 3377
Fragments arranged by size:
3377 631 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4256
NgoMIV G'CCGG_C
Cuts at: 392 2435 392
Size: 2043 2213
NspI r_CATG'y
Cuts at: 1309 1809 2716 1309
Size: 500 907 2849
Fragments arranged by size:
2849 907 500
PasI CC'CwG_GG
Cuts at: 737 737
Size: 4256
PciI A'CATG_T
Cuts at: 2712 2712
Size: 4256
PfoI T'CCnGG_A
Cuts at: 257 257
Size: 4256
PmeI GTTT'AAAC
Cuts at: 1255 1255
Size: 4256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3427 3395
Size: 32 4224
PpuMI rG'GwC_Cy
Cuts at: 195 1063 195
Size: 868 3388
PspOMI G'GGCC_C
Cuts at: 1135 1186 1135
Size: 51 4205
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 835 867 835
Size: 32 4224
PstI C_TGCA'G
Cuts at: 1019 1303 1988 1019
Size: 284 685 3287
Fragments arranged by size:
3287 685 284
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4256
PvuII CAG'CTG
Cuts at: 177 1681 2041 177
Size: 1504 360 2392
Fragments arranged by size:
2392 1504 360
RsrII CG'GwC_CG
Cuts at: 2451 2451
Size: 4256
SacI G_AGCT'C
Cuts at: 1270 1270
Size: 4256
SalI G'TCGA_C
Cuts at: 1293 1293
Size: 4256
SanDI GG'GwC_CC
Cuts at: 1063 1063
Size: 4256
SapI GCTCTTCn'nnn_
Cuts at: 523 2279 2489 523
Size: 1756 210 2290
Fragments arranged by size:
2290 1756 210
SbfI CC_TGCA'GG
Cuts at: 1303 1303
Size: 4256
ScaI AGT'ACT
Cuts at: 3773 3773
Size: 4256
SfcI C'TryA_G
Cuts at: 33 1015 1299 1984 2977 3168 33
Size: 982 284 685 993 191 1121
Fragments arranged by size:
1121 993 982 685 284 191
SfoI GGC'GCC
Cuts at: 1057 1688 1936 1057
Size: 631 248 3377
Fragments arranged by size:
3377 631 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
SgrAI Cr'CCGG_yG
Cuts at: 392 392
Size: 4256
SmaI CCC'GGG
Cuts at: 1111 1132 1278 1111
Size: 21 146 4089
Fragments arranged by size:
4089 146 21
SmlI C'TyrA_G
Cuts at: 769 1141 1204 1411 2326 2818 3080 769
Size: 372 63 207 915 492 262 1945
Fragments arranged by size:
1945 915 492 372 262 207 63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4256
SphI G_CATG'C
Cuts at: 1309 1809 1309
Size: 500 3756
StyI C'CwwG_G
Cuts at: 107 464 494 1390 1744 107
Size: 357 30 896 354 2619
Fragments arranged by size:
2619 896 357 354 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 666 1839 1972 2431 2640 666
Size: 1173 133 459 209 2282
Fragments arranged by size:
2282 1173 459 209 133
TatI w'GTAC_w
Cuts at: 419 3771 419
Size: 3352 904
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 662 694 3773 3805 662
Size: 32 3079 32 1113
Fragments arranged by size:
3079 1113 32 32
Tth111I GACn'n_nGTC
Cuts at: 2053 2053
Size: 4256
XbaI T'CTAG_A
Cuts at: 1287 1287
Size: 4256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 486 937 967 1751 486
Size: 451 30 784 2991
Fragments arranged by size:
2991 784 451 30
XmaI C'CCGG_G
Cuts at: 1109 1130 1276 1109
Size: 21 146 4089
Fragments arranged by size:
4089 146 21
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PshAI PsiI PspXI
SacII SexAI SfiI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI