Restriction map for
pF1KB5263
(Circular) (Six-base) MAPSORT of: pF1KB5263.seq Check: 7914 from: 1 to: 4250
pF1KB5263 4250 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 646 646
Size: 4250
AccI GT'mk_AC
Cuts at: 461 1288 461
Size: 827 3423
Acc65I G'GTAC_C
Cuts at: 734 1266 734
Size: 532 3718
AclI AA'CG_TT
Cuts at: 4090 4090
Size: 4250
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 2075 2507 2860 3522 1207
Size: 868 432 353 662 1935
Fragments arranged by size:
1935 868 662 432 353
AfeI AGC'GCT
Cuts at: 1079 1079
Size: 4250
AflII C'TTAA_G
Cuts at: 646 646
Size: 4250
AflIII A'CryG_T
Cuts at: 1602 3402 3683 1602
Size: 1800 281 2169
Fragments arranged by size:
2169 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 792 792
Size: 4250
AlwNI CAG_nnn'CTG
Cuts at: 381 477 519 558 957 1555 2993 381
Size: 96 42 39 399 598 1438 1638
Fragments arranged by size:
1638 1438 598 399 96 42 39
ApaI G_GGCC'C
Cuts at: 226 744 226
Size: 518 3732
ApaLI G'TGCA_C
Cuts at: 3088 3088
Size: 4250
ApoI r'AATT_y
Cuts at: 1254 3603 3859 1254
Size: 2349 256 1645
Fragments arranged by size:
2349 1645 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4250
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4250
AvaI C'yCGr_G
Cuts at: 171 180 1270 171
Size: 9 1090 3151
Fragments arranged by size:
3151 1090 9
BamHI G'GATC_C
Cuts at: 1275 1275
Size: 4250
BanI G'GyrC_C
Cuts at: 734 1266 1680 1928 1963 734
Size: 532 414 248 35 3021
Fragments arranged by size:
3021 532 414 248 35
BanII G_rGCy'C
Cuts at: 149 226 744 895 1142 1196 1264 2294 149
Size: 77 518 151 247 54 68 1030 2105
Fragments arranged by size:
2105 1030 518 247 151 77 68 54
BbeI G_GCGC'C
Cuts at: 1684 1932 1684
Size: 248 4002
BciVI GTATCCnnnnn_n'
Cuts at: 2148 3204 4250 2148
Size: 1056 1046 2148
Fragments arranged by size:
2148 1056 1046
BclI T'GATC_A
Cuts at: 1770 1770
Size: 4250
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4250
BglII A'GATC_T
Cuts at: 603 1765 3802 603
Size: 1162 2037 1051
Fragments arranged by size:
2037 1162 1051
BlpI GC'TnA_GC
Cuts at: 520 1362 520
Size: 842 3408
Bme1580I G_kGCm'C
Cuts at: 226 744 1875 1968 3092 226
Size: 518 1131 93 1124 1384
Fragments arranged by size:
1384 1131 1124 518 93
BmgBI CAC'GTC
Cuts at: 219 219
Size: 4250
BmrI ACTGGGnnnn_n'
Cuts at: 1462 1543 1878 4022 1462
Size: 81 335 2144 1690
Fragments arranged by size:
2144 1690 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 840 2617 840
Size: 1777 2473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1117 1255 1117
Size: 138 4112
Bpu10I CC'TnA_GC
Cuts at: 125 686 125
Size: 561 3689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 1426 2305 3019 3317 553
Size: 873 879 714 298 1486
Fragments arranged by size:
1486 879 873 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3954 3954
Size: 4250
BsaAI yAC'GTr
Cuts at: 294 1603 294
Size: 1309 2941
BsaBI GATnn'nnATC
Cuts at: 6 376 1764 3801 6
Size: 370 1388 2037 455
Fragments arranged by size:
2037 1388 455 370
BsaHI Gr'CG_yC
Cuts at: 433 1681 1929 2631 4127 433
Size: 1248 248 702 1496 556
Fragments arranged by size:
1496 1248 702 556 248
BsaWI w'CCGG_w
Cuts at: 1031 1439 1960 2638 3049 3196 3470 1031
Size: 408 521 678 411 147 274 1811
Fragments arranged by size:
1811 678 521 411 408 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 565 595 565
Size: 30 4220
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1150 1150
Size: 4250
BseYI C'CCAG_C
Cuts at: 1675 2433 3098 3506 1675
Size: 758 665 408 2419
Fragments arranged by size:
2419 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 490
Size: 4250
BsiEI CG_ry'CG
Cuts at: 104 1792 1838 3068 104
Size: 1688 46 1230 1286
Fragments arranged by size:
1688 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 596 1196 1264 2042 2232 3092 596
Size: 600 68 778 190 860 1754
Fragments arranged by size:
1754 860 778 600 190 68
BsiWI C'GTAC_G
Cuts at: 263 263
Size: 4250
BsmI GAATG_Cn'
Cuts at: 806 806
Size: 4250
Bsp1286I G_dGCh'C
Cuts at: 149 226 596 744 895 1142 1196 1264 1875
Size: 77 370 148 151 247 54 68 611
Cuts at: 1875 1968 2042 2232 2294 3092 149
Size: 93 74 190 62 798 1307
Fragments arranged by size:
1307 798 611 370 247 190 151 148
93 77 74 68 62 54
BspHI T'CATG_A
Cuts at: 2682 2682
Size: 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 629 646 1031 1300 1514 1816 2197 629
Size: 17 385 269 214 302 381 2682
Fragments arranged by size:
2682 385 381 302 269 214 17
BsrBI CCG'CTC
Cuts at: 50 1182 2542 2596 4080 4245 50
Size: 1132 1360 54 1484 165 55
Fragments arranged by size:
1484 1360 1132 165 55 54
BsrDI GCAATG_nn'
Cuts at: 531 2162 531
Size: 1631 2619
BsrFI r'CCGG_y
Cuts at: 700 830 2248 2429 700
Size: 130 1418 181 2521
Fragments arranged by size:
2521 1418 181 130
BsrGI T'GTAC_A
Cuts at: 494 494
Size: 4250
BssSI C'ACGA_G
Cuts at: 2521 3229 2521
Size: 708 3542
BstAPI GCAn_nnn'nTGC
Cuts at: 1627 1627
Size: 4250
BstBI TT'CG_AA
Cuts at: 335 2611 335
Size: 2276 1974
BstXI CCAn_nnnn'nTGG
Cuts at: 1161 1161
Size: 4250
BstYI r'GATC_y
Cuts at: 1 371 377 554 603 1275 1742 1765 2100
Size: 370 6 177 49 672 467 23 335
Cuts at: 2100 2346 2652 2664 2750 3802 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 672 467 449 370 335 306 246
177 86 49 23 12 6
Bsu36I CC'TnA_GG
Cuts at: 1175 1175
Size: 4250
BtgI C'CryG_G
Cuts at: 107 1154 107
Size: 1047 3203
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2200 2381 2544 2200
Size: 181 163 3906
Fragments arranged by size:
3906 181 163
BtsI GCAGTG_nn'
Cuts at: 1493 1493
Size: 4250
DraI TTT'AAA
Cuts at: 1249 1249
Size: 4250
DrdI GACnn_nn'nnGTC
Cuts at: 1956 3300 1956
Size: 1344 2906
EaeI y'GGCC_r
Cuts at: 311 1130 1835 2009 2400 2427 3772 311
Size: 819 705 174 391 27 1345 789
Fragments arranged by size:
1345 819 789 705 391 174 27
EagI C'GGCC_G
Cuts at: 1835 1835
Size: 4250
EarI CTCTTCn'nnn_
Cuts at: 2273 2483 2273
Size: 210 4040
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 382 573 2458 3200 3346 4060 382
Size: 191 1885 742 146 714 572
Fragments arranged by size:
1885 742 714 572 191 146
EcoICRI GAG'CTC
Cuts at: 1194 1262 1194
Size: 68 4182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1117 1207 1255 2075 2507 2860 3522 1117
Size: 90 48 820 432 353 662 1845
Fragments arranged by size:
1845 820 662 432 353 90 48
EcoNI CCTnn'n_nnAGG
Cuts at: 1174 1174
Size: 4250
EcoO109I rG'GnC_Cy
Cuts at: 177 474 564 741 909 1239 1389 177
Size: 297 90 177 168 330 150 3038
Fragments arranged by size:
3038 330 297 177 168 150 90
EcoRI G'AATT_C
Cuts at: 1254 1254
Size: 4250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2269 2269
Size: 4250
FspI TGC'GCA
Cuts at: 298 2031 298
Size: 1733 2517
HaeII r_GCGC'y
Cuts at: 1081 1684 1932 3162 3933 1081
Size: 603 248 1230 771 1398
Fragments arranged by size:
1398 1230 771 603 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 666 698 778 810 2092 2122 2124 2154 666
Size: 32 80 32 1282 30 2 30 2762
Fragments arranged by size:
2762 1282 80 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1040 1289 1040
Size: 249 4001
HindIII A'AGCT_T
Cuts at: 988 988
Size: 4250
HpaI GTT'AAC
Cuts at: 1040 1040
Size: 4250
KasI G'GCGC_C
Cuts at: 1680 1928 1680
Size: 248 4002
KpnI G_GTAC'C
Cuts at: 738 1270 738
Size: 532 3718
MluI A'CGCG_T
Cuts at: 3683 3683
Size: 4250
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 641 3009 3193 641
Size: 2368 184 1698
Fragments arranged by size:
2368 1698 184
MscI TGG'CCA
Cuts at: 313 2011 313
Size: 1698 2552
MslI CAynn'nnrTG
Cuts at: 401 928 2239 2366 401
Size: 527 1311 127 2285
Fragments arranged by size:
2285 1311 527 127
MspA1I CmG'CkG
Cuts at: 519 1361 1675 2035 2819 3064 519
Size: 842 314 360 784 245 1705
Fragments arranged by size:
1705 842 784 360 314 245
NaeI GCC'GGC
Cuts at: 2431 2431
Size: 4250
NarI GG'CG_CC
Cuts at: 1681 1929 1681
Size: 248 4002
NcoI C'CATG_G
Cuts at: 107 1154 107
Size: 1047 3203
NgoMIV G'CCGG_C
Cuts at: 2429 2429
Size: 4250
NspI r_CATG'y
Cuts at: 1303 1803 3406 1303
Size: 500 1603 2147
Fragments arranged by size:
2147 1603 500
PciI A'CATG_T
Cuts at: 3402 3402
Size: 4250
PflMI CCAn_nnn'nTGG
Cuts at: 879 879
Size: 4250
PfoI T'CCnGG_A
Cuts at: 365 1202 365
Size: 837 3413
PmeI GTTT'AAAC
Cuts at: 1249 1249
Size: 4250
PmlI CAC'GTG
Cuts at: 294 294
Size: 4250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2696 2728 2696
Size: 32 4218
PpuMI rG'GwC_Cy
Cuts at: 177 474 909 177
Size: 297 435 3518
Fragments arranged by size:
3518 435 297
PspOMI G'GGCC_C
Cuts at: 222 740 222
Size: 518 3732
PstI C_TGCA'G
Cuts at: 1297 1982 1297
Size: 685 3565
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4250
PvuII CAG'CTG
Cuts at: 519 1675 2035 519
Size: 1156 360 2734
Fragments arranged by size:
2734 1156 360
RsrII CG'GwC_CG
Cuts at: 2445 2445
Size: 4250
SacI G_AGCT'C
Cuts at: 1196 1264 1196
Size: 68 4182
SalI G'TCGA_C
Cuts at: 1287 1287
Size: 4250
SanDI GG'GwC_CC
Cuts at: 177 177
Size: 4250
SapI GCTCTTCn'nnn_
Cuts at: 2273 2483 2273
Size: 210 4040
SbfI CC_TGCA'GG
Cuts at: 1297 1297
Size: 4250
ScaI AGT'ACT
Cuts at: 1105 3767 1105
Size: 2662 1588
SfcI C'TryA_G
Cuts at: 33 949 1293 1978 2946 3137 33
Size: 916 344 685 968 191 1146
Fragments arranged by size:
1146 968 916 685 344 191
SfoI GGC'GCC
Cuts at: 1682 1930 1682
Size: 248 4002
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4250
SmaI CCC'GGG
Cuts at: 173 182 1272 173
Size: 9 1090 3151
Fragments arranged by size:
3151 1090 9
SmlI C'TyrA_G
Cuts at: 568 646 1405 2320 3034 3296 568
Size: 78 759 915 714 262 1522
Fragments arranged by size:
1522 915 759 714 262 78
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4250
SphI G_CATG'C
Cuts at: 1303 1803 1303
Size: 500 3750
StyI C'CwwG_G
Cuts at: 107 764 971 1154 1384 1738 107
Size: 657 207 183 230 354 2619
Fragments arranged by size:
2619 657 354 230 207 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1833 1966 2425 2634 1833
Size: 133 459 209 3449
Fragments arranged by size:
3449 459 209 133
TatI w'GTAC_w
Cuts at: 245 494 572 1103 3765 245
Size: 249 78 531 2662 730
Fragments arranged by size:
2662 730 531 249 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3767 3799 3767
Size: 32 4218
Tth111I GACn'n_nGTC
Cuts at: 2047 2047
Size: 4250
XbaI T'CTAG_A
Cuts at: 1281 1281
Size: 4250
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 972 1745 972
Size: 773 3477
XmaI C'CCGG_G
Cuts at: 171 180 1270 171
Size: 9 1090 3151
Fragments arranged by size:
3151 1090 9
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AgeI AleI AlfI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmtI BsmBI BspEI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI