Restriction map for
pF1KB5268
(Circular) (Six-base) MAPSORT of: pF1KB5268.seq Check: 4844 from: 1 to: 4217
pF1KB5268 4217 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 1255 1255
Size: 4217
Acc65I G'GTAC_C
Cuts at: 1233 1233
Size: 4217
AclI AA'CG_TT
Cuts at: 4057 4057
Size: 4217
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2042 2474 3221 3489 2042
Size: 432 747 268 2770
Fragments arranged by size:
2770 747 432 268
AfeI AGC'GCT
Cuts at: 839 839
Size: 4217
AflIII A'CryG_T
Cuts at: 1569 2673 3650 1569
Size: 1104 977 2136
Fragments arranged by size:
2136 1104 977
AgeI A'CCGG_T
Cuts at: 2605 2605
Size: 4217
AleI CACnn'nnGTG
Cuts at: 511 511
Size: 4217
AlwNI CAG_nnn'CTG
Cuts at: 1522 3089 1522
Size: 1567 2650
ApaI G_GGCC'C
Cuts at: 361 620 639 1117 361
Size: 259 19 478 3461
Fragments arranged by size:
3461 478 259 19
ApaLI G'TGCA_C
Cuts at: 460 1028 2987 460
Size: 568 1959 1690
Fragments arranged by size:
1959 1690 568
ApoI r'AATT_y
Cuts at: 1221 3570 3826 1221
Size: 2349 256 1612
Fragments arranged by size:
2349 1612 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4217
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
AvaI C'yCGr_G
Cuts at: 334 418 613 811 1153 1237 334
Size: 84 195 198 342 84 3314
Fragments arranged by size:
3314 342 198 195 84 84
BamHI G'GATC_C
Cuts at: 1242 1242
Size: 4217
BanI G'GyrC_C
Cuts at: 676 894 1070 1233 1647 1895 1930 676
Size: 218 176 163 414 248 35 2963
Fragments arranged by size:
2963 414 248 218 176 163 35
BanII G_rGCy'C
Cuts at: 361 620 639 1117 1177 1231 2261 361
Size: 259 19 478 60 54 1030 2317
Fragments arranged by size:
2317 1030 478 259 60 54 19
BbeI G_GCGC'C
Cuts at: 680 898 1651 1899 680
Size: 218 753 248 2998
Fragments arranged by size:
2998 753 248 218
BciVI GTATCCnnnnn_n'
Cuts at: 2115 2876 4217 2115
Size: 761 1341 2115
Fragments arranged by size:
2115 1341 761
BclI T'GATC_A
Cuts at: 1737 1737
Size: 4217
BglI GCCn_nnn'nGGC
Cuts at: 336 517 624 643 729 798 925 949 958
Size: 181 107 19 86 69 127 24 9
Cuts at: 958 336
Size: 3595
Fragments arranged by size:
3595 181 127 107 86 69 24 19
9
BglII A'GATC_T
Cuts at: 1732 3769 1732
Size: 2037 2180
BlpI GC'TnA_GC
Cuts at: 1329 1329
Size: 4217
Bme1580I G_kGCm'C
Cuts at: 192 361 464 620 639 1032 1083 1117 1842
Size: 169 103 156 19 393 51 34 725
Cuts at: 1842 1935 2991 192
Size: 93 1056 1418
Fragments arranged by size:
1418 1056 725 393 169 156 103 93
51 34 19
BmrI ACTGGGnnnn_n'
Cuts at: 1018 1429 1510 1845 3989 1018
Size: 411 81 335 2144 1246
Fragments arranged by size:
2144 1246 411 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2584 2584
Size: 4217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1081 1124 1081
Size: 43 4174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1393 2272 2764 3062 1393
Size: 879 492 298 2548
Fragments arranged by size:
2548 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1095 3921 1095
Size: 2826 1391
BsaAI yAC'GTr
Cuts at: 1570 1570
Size: 4217
BsaBI GATnn'nnATC
Cuts at: 6 1731 3768 6
Size: 1725 2037 455
Fragments arranged by size:
2037 1725 455
BsaHI Gr'CG_yC
Cuts at: 677 895 1648 1896 2598 4094 677
Size: 218 753 248 702 1496 800
Fragments arranged by size:
1496 800 753 702 248 218
BsaWI w'CCGG_w
Cuts at: 1406 1927 2605 2879 3026 3437 1406
Size: 521 678 274 147 411 2186
Fragments arranged by size:
2186 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 195 309 339 534 604 834 1642 2400 2977
Size: 114 30 195 70 230 808 758 577
Cuts at: 2977 3473 195
Size: 496 939
Fragments arranged by size:
939 808 758 577 496 230 195 114
70 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 1038 396
Size: 642 3575
BsiEI CG_ry'CG
Cuts at: 104 293 487 628 733 1759 1805 3013 104
Size: 189 194 141 105 1026 46 1208 1308
Fragments arranged by size:
1308 1208 1026 194 189 141 105 46
BsiHKAI G_wGCw'C
Cuts at: 464 500 506 1032 1231 2009 2199 2991 464
Size: 36 6 526 199 778 190 792 1690
Fragments arranged by size:
1690 792 778 526 199 190 36 6
Bsp1286I G_dGCh'C
Cuts at: 192 361 464 500 506 620 639 1032 1083
Size: 169 103 36 6 114 19 393 51
Cuts at: 1083 1117 1177 1231 1842 1935 2009 2199 2261
Size: 34 60 54 611 93 74 190 62
Cuts at: 2261 2991 192
Size: 730 1418
Fragments arranged by size:
1418 730 611 393 190 169 114 103
93 74 62 60 54 51 36 34
19 6
BspEI T'CCGG_A
Cuts at: 3437 3437
Size: 4217
BspHI T'CATG_A
Cuts at: 3393 3393
Size: 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 290 1267 1481 1783 2164 290
Size: 977 214 302 381 2343
Fragments arranged by size:
2343 977 381 302 214
BsrBI CCG'CTC
Cuts at: 50 379 397 630 755 795 2509 2563 4047
Size: 329 18 233 125 40 1714 54 1484
Cuts at: 4047 4212 50
Size: 165 55
Fragments arranged by size:
1714 1484 329 233 165 125 55 54
40 18
BsrDI GCAATG_nn'
Cuts at: 1096 2129 1096
Size: 1033 3184
BsrFI r'CCGG_y
Cuts at: 405 732 2215 2396 2605 405
Size: 327 1483 181 209 2017
Fragments arranged by size:
2017 1483 327 209 181
BssHII G'CGCG_C
Cuts at: 206 539 736 206
Size: 333 197 3687
Fragments arranged by size:
3687 333 197
BssSI C'ACGA_G
Cuts at: 499 2488 2846 499
Size: 1989 358 1870
Fragments arranged by size:
1989 1870 358
BstAPI GCAn_nnn'nTGC
Cuts at: 468 1594 468
Size: 1126 3091
BstBI TT'CG_AA
Cuts at: 2578 2578
Size: 4217
BstYI r'GATC_y
Cuts at: 1 1242 1709 1732 2067 2313 3325 3411 3423
Size: 1241 467 23 335 246 1012 86 12
Cuts at: 3423 3769 1
Size: 346 449
Fragments arranged by size:
1241 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 718 718
Size: 4217
BtgI C'CryG_G
Cuts at: 107 521 727 845 911 1075 107
Size: 414 206 118 66 164 3249
Fragments arranged by size:
3249 414 206 164 118 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2167 2348 2511 2167
Size: 181 163 3873
Fragments arranged by size:
3873 181 163
BtsI GCAGTG_nn'
Cuts at: 190 1030 1460 190
Size: 840 430 2947
Fragments arranged by size:
2947 840 430
DraI TTT'AAA
Cuts at: 1216 1216
Size: 4217
DrdI GACnn_nn'nnGTC
Cuts at: 1923 2781 1923
Size: 858 3359
EaeI y'GGCC_r
Cuts at: 110 290 320 425 472 484 625 730 842
Size: 180 30 105 47 12 141 105 112
Cuts at: 842 1802 1976 2367 2394 3739 110
Size: 960 174 391 27 1345 588
Fragments arranged by size:
1345 960 588 391 180 174 141 112
105 105 47 30 27 12
EagI C'GGCC_G
Cuts at: 290 484 625 730 1802 290
Size: 194 141 105 1072 2705
Fragments arranged by size:
2705 1072 194 141 105
EarI CTCTTCn'nnn_
Cuts at: 2240 2450 2240
Size: 210 4007
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 381 1185 2425 2735 2881 4027 381
Size: 804 1240 310 146 1146 571
Fragments arranged by size:
1240 1146 804 571 310 146
EcoICRI GAG'CTC
Cuts at: 1229 1229
Size: 4217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1081 1124 2042 2474 3221 3489 1081
Size: 43 918 432 747 268 1809
Fragments arranged by size:
1809 918 747 432 268 43
EcoNI CCTnn'n_nnAGG
Cuts at: 1121 1121
Size: 4217
EcoO109I rG'GnC_Cy
Cuts at: 358 609 617 636 722 743 1113 1114 1126
Size: 251 8 19 86 21 370 1 12
Cuts at: 1126 1356 358
Size: 230 3219
Fragments arranged by size:
3219 370 251 230 86 21 19 12
8 1
EcoRI G'AATT_C
Cuts at: 1221 1221
Size: 4217
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 4217
FspI TGC'GCA
Cuts at: 229 1998 229
Size: 1769 2448
HaeII r_GCGC'y
Cuts at: 680 841 898 993 1651 1899 2921 3900 680
Size: 161 57 95 658 248 1022 979 997
Fragments arranged by size:
1022 997 979 658 248 161 95 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2059 2089 2091 2121 2059
Size: 30 2 30 4155
Fragments arranged by size:
4155 30 30 2
HincII GTy'rAC
Cuts at: 1256 1256
Size: 4217
KasI G'GCGC_C
Cuts at: 676 894 1647 1895 676
Size: 218 753 248 2998
Fragments arranged by size:
2998 753 248 218
KpnI G_GTAC'C
Cuts at: 1237 1237
Size: 4217
MluI A'CGCG_T
Cuts at: 3650 3650
Size: 4217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2888 3072 2888
Size: 184 4033
MscI TGG'CCA
Cuts at: 112 1978 112
Size: 1866 2351
MslI CAynn'nnrTG
Cuts at: 511 694 2206 2333 511
Size: 183 1512 127 2395
Fragments arranged by size:
2395 1512 183 127
MspA1I CmG'CkG
Cuts at: 172 309 468 523 913 1077 1328 1642 2002
Size: 137 159 55 390 164 251 314 360
Cuts at: 2002 3015 3260 172
Size: 1013 245 1129
Fragments arranged by size:
1129 1013 390 360 314 251 245 164
159 137 55
NaeI GCC'GGC
Cuts at: 407 734 2398 407
Size: 327 1664 2226
Fragments arranged by size:
2226 1664 327
NarI GG'CG_CC
Cuts at: 677 895 1648 1896 677
Size: 218 753 248 2998
Fragments arranged by size:
2998 753 248 218
NcoI C'CATG_G
Cuts at: 107 107
Size: 4217
NgoMIV G'CCGG_C
Cuts at: 405 732 2396 405
Size: 327 1664 2226
Fragments arranged by size:
2226 1664 327
NotI GC'GGCC_GC
Cuts at: 290 484 290
Size: 194 4023
NspI r_CATG'y
Cuts at: 1270 1770 2677 1270
Size: 500 907 2810
Fragments arranged by size:
2810 907 500
PciI A'CATG_T
Cuts at: 2673 2673
Size: 4217
PmeI GTTT'AAAC
Cuts at: 1216 1216
Size: 4217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3356 3388 3356
Size: 32 4185
PpuMI rG'GwC_Cy
Cuts at: 743 743
Size: 4217
PspOMI G'GGCC_C
Cuts at: 357 616 635 1113 357
Size: 259 19 478 3461
Fragments arranged by size:
3461 478 259 19
PstI C_TGCA'G
Cuts at: 989 1264 1949 989
Size: 275 685 3257
Fragments arranged by size:
3257 685 275
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4217
PvuII CAG'CTG
Cuts at: 172 1642 2002 172
Size: 1470 360 2387
Fragments arranged by size:
2387 1470 360
RsrII CG'GwC_CG
Cuts at: 2412 2412
Size: 4217
SacI G_AGCT'C
Cuts at: 1231 1231
Size: 4217
SacII CC_GC'GG
Cuts at: 524 914 1078 524
Size: 390 164 3663
Fragments arranged by size:
3663 390 164
SalI G'TCGA_C
Cuts at: 1254 1254
Size: 4217
SanDI GG'GwC_CC
Cuts at: 743 743
Size: 4217
SapI GCTCTTCn'nnn_
Cuts at: 2240 2450 2240
Size: 210 4007
SbfI CC_TGCA'GG
Cuts at: 1264 1264
Size: 4217
ScaI AGT'ACT
Cuts at: 3734 3734
Size: 4217
SfcI C'TryA_G
Cuts at: 33 985 1260 1945 2938 3129 33
Size: 952 275 685 993 191 1121
Fragments arranged by size:
1121 993 952 685 275 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 624 643 729 624
Size: 19 86 4112
Fragments arranged by size:
4112 86 19
SfoI GGC'GCC
Cuts at: 678 896 1649 1897 678
Size: 218 753 248 2998
Fragments arranged by size:
2998 753 248 218
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
SmaI CCC'GGG
Cuts at: 336 813 1239 336
Size: 477 426 3314
Fragments arranged by size:
3314 477 426
SmlI C'TyrA_G
Cuts at: 1372 2287 2779 3041 1372
Size: 915 492 262 2548
Fragments arranged by size:
2548 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4217
SphI G_CATG'C
Cuts at: 1270 1770 1270
Size: 500 3717
SrfI GCCC'GGGC
Cuts at: 336 336
Size: 4217
StyI C'CwwG_G
Cuts at: 107 1351 1705 107
Size: 1244 354 2619
Fragments arranged by size:
2619 1244 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 585 1800 1933 2392 2601 585
Size: 1215 133 459 209 2201
Fragments arranged by size:
2201 1215 459 209 133
TatI w'GTAC_w
Cuts at: 3732 3732
Size: 4217
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3734 3766 3734
Size: 32 4185
Tth111I GACn'n_nGTC
Cuts at: 2014 2014
Size: 4217
XbaI T'CTAG_A
Cuts at: 1248 1248
Size: 4217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 306 1712 306
Size: 1406 2811
XmaI C'CCGG_G
Cuts at: 334 811 1237 334
Size: 477 426 3314
Fragments arranged by size:
3314 477 426
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SexAI
SgrAI SnaBI SspI StuI SwaI XhoI XmnI ZraI