Restriction map for
pF1KB5271
(Circular) (Six-base) MAPSORT of: pF1KB5271.seq Check: 2254 from: 1 to: 4364
pF1KB5271 4364 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1155 1155
Size: 4364
AccI GT'mk_AC
Cuts at: 1257 1402 1257
Size: 145 4219
Acc65I G'GTAC_C
Cuts at: 1380 1380
Size: 4364
AclI AA'CG_TT
Cuts at: 4204 4204
Size: 4364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 787 2189 2621 3368 3636 393
Size: 394 1402 432 747 268 1121
Fragments arranged by size:
1402 1121 747 432 394 268
AflIII A'CryG_T
Cuts at: 1716 2820 3797 1716
Size: 1104 977 2283
Fragments arranged by size:
2283 1104 977
AgeI A'CCGG_T
Cuts at: 2752 2752
Size: 4364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 962 962
Size: 4364
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 560 592 560
Size: 32 4332
AlwNI CAG_nnn'CTG
Cuts at: 1669 3236 1669
Size: 1567 2797
ApaLI G'TGCA_C
Cuts at: 3134 3134
Size: 4364
ApoI r'AATT_y
Cuts at: 316 796 911 1011 1133 1368 3717 3973 316
Size: 480 115 100 122 235 2349 256 707
Fragments arranged by size:
2349 707 480 256 235 122 115 100
AseI AT'TA_AT
Cuts at: 20 506 20
Size: 486 3878
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
AvaI C'yCGr_G
Cuts at: 1384 1384
Size: 4364
AvrII C'CTAG_G
Cuts at: 603 603
Size: 4364
BamHI G'GATC_C
Cuts at: 1389 1389
Size: 4364
BanI G'GyrC_C
Cuts at: 1380 1794 2042 2077 1380
Size: 414 248 35 3667
Fragments arranged by size:
3667 414 248 35
BanII G_rGCy'C
Cuts at: 1378 2408 1378
Size: 1030 3334
BbeI G_GCGC'C
Cuts at: 1798 2046 1798
Size: 248 4116
BbsI GAAGACnn'nnnn_
Cuts at: 151 151
Size: 4364
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4330
BciVI GTATCCnnnnn_n'
Cuts at: 2262 3023 4364 2262
Size: 761 1341 2262
Fragments arranged by size:
2262 1341 761
BclI T'GATC_A
Cuts at: 1884 1884
Size: 4364
BglII A'GATC_T
Cuts at: 1879 3916 1879
Size: 2037 2327
BlpI GC'TnA_GC
Cuts at: 1476 1476
Size: 4364
Bme1580I G_kGCm'C
Cuts at: 1989 2082 3138 1989
Size: 93 1056 3215
Fragments arranged by size:
3215 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 568 1576 1657 1992 4136 568
Size: 1008 81 335 2144 796
Fragments arranged by size:
2144 1008 796 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 436 843 2731 436
Size: 407 1888 2069
Fragments arranged by size:
2069 1888 407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 218
Size: 4364
Bpu10I CC'TnA_GC
Cuts at: 254 254
Size: 4364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1540 2419 2911 3209 1540
Size: 879 492 298 2695
Fragments arranged by size:
2695 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 356 4068 356
Size: 3712 652
BsaAI yAC'GTr
Cuts at: 1717 1717
Size: 4364
BsaBI GATnn'nnATC
Cuts at: 6 1878 3915 6
Size: 1872 2037 455
Fragments arranged by size:
2037 1872 455
BsaHI Gr'CG_yC
Cuts at: 1795 2043 2745 4241 1795
Size: 248 702 1496 1918
Fragments arranged by size:
1918 1496 702 248
BsaWI w'CCGG_w
Cuts at: 714 1553 2074 2752 3026 3173 3584 714
Size: 839 521 678 274 147 411 1494
Fragments arranged by size:
1494 839 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 244 1789 2547 3124 3620 244
Size: 1545 758 577 496 988
Fragments arranged by size:
1545 988 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 155 1906 1952 3160 104
Size: 51 1751 46 1208 1308
Fragments arranged by size:
1751 1308 1208 51 46
BsiHKAI G_wGCw'C
Cuts at: 597 1378 2156 2346 3138 597
Size: 781 778 190 792 1823
Fragments arranged by size:
1823 792 781 778 190
Bsp1286I G_dGCh'C
Cuts at: 597 1378 1989 2082 2156 2346 2408 3138 597
Size: 781 611 93 74 190 62 730 1823
Fragments arranged by size:
1823 781 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 714 3584 714
Size: 2870 1494
BspHI T'CATG_A
Cuts at: 3540 3540
Size: 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1155 1414 1628 1930 2311 1155
Size: 259 214 302 381 3208
Fragments arranged by size:
3208 381 302 259 214
BsrBI CCG'CTC
Cuts at: 50 114 134 2656 2710 4194 4359 50
Size: 64 20 2522 54 1484 165 55
Fragments arranged by size:
2522 1484 165 64 55 54 20
BsrDI GCAATG_nn'
Cuts at: 2276 2276
Size: 4364
BsrFI r'CCGG_y
Cuts at: 2362 2543 2752 2362
Size: 181 209 3974
Fragments arranged by size:
3974 209 181
BssSI C'ACGA_G
Cuts at: 425 2635 2993 425
Size: 2210 358 1796
Fragments arranged by size:
2210 1796 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1741 1741
Size: 4364
BstBI TT'CG_AA
Cuts at: 1131 2725 1131
Size: 1594 2770
BstYI r'GATC_y
Cuts at: 1 1389 1856 1879 2214 2460 3472 3558 3570
Size: 1388 467 23 335 246 1012 86 12
Cuts at: 3570 3916 1
Size: 346 449
Fragments arranged by size:
1388 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1258 1258
Size: 4364
BtgI C'CryG_G
Cuts at: 515 515
Size: 4364
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2314 2495 2658 2314
Size: 181 163 4020
Fragments arranged by size:
4020 181 163
BtsI GCAGTG_nn'
Cuts at: 1607 1607
Size: 4364
DraI TTT'AAA
Cuts at: 1363 1363
Size: 4364
DrdI GACnn_nn'nnGTC
Cuts at: 2070 2928 2070
Size: 858 3506
EaeI y'GGCC_r
Cuts at: 1949 2123 2514 2541 3886 1949
Size: 174 391 27 1345 2427
Fragments arranged by size:
2427 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1949 1949
Size: 4364
EarI CTCTTCn'nnn_
Cuts at: 494 623 929 1330 2387 2597 494
Size: 129 306 401 1057 210 2261
Fragments arranged by size:
2261 1057 401 306 210 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2572 2882 3028 4174 2572
Size: 310 146 1146 2762
Fragments arranged by size:
2762 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1376 1376
Size: 4364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 393 787 2189 2621 3368 3636 218
Size: 175 394 1402 432 747 268 946
Fragments arranged by size:
1402 946 747 432 394 268 175
EcoNI CCTnn'n_nnAGG
Cuts at: 169 169
Size: 4364
EcoO109I rG'GnC_Cy
Cuts at: 123 1503 123
Size: 1380 2984
EcoRI G'AATT_C
Cuts at: 316 1133 1368 316
Size: 817 235 3312
Fragments arranged by size:
3312 817 235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1225 2383 1225
Size: 1158 3206
FspI TGC'GCA
Cuts at: 2145 2145
Size: 4364
HaeII r_GCGC'y
Cuts at: 1798 2046 3068 4047 1798
Size: 248 1022 979 2115
Fragments arranged by size:
2115 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 408 439 440 471 767 799 2206 2236 2238
Size: 31 1 31 296 32 1407 30 2
Cuts at: 2238 2268 408
Size: 30 2504
Fragments arranged by size:
2504 1407 296 32 31 31 30 30
2 1
HincII GTy'rAC
Cuts at: 663 846 1403 663
Size: 183 557 3624
Fragments arranged by size:
3624 557 183
KasI G'GCGC_C
Cuts at: 1794 2042 1794
Size: 248 4116
KpnI G_GTAC'C
Cuts at: 1384 1384
Size: 4364
MluI A'CGCG_T
Cuts at: 3797 3797
Size: 4364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 611 3035 3219 611
Size: 2424 184 1756
Fragments arranged by size:
2424 1756 184
MscI TGG'CCA
Cuts at: 2125 2125
Size: 4364
MslI CAynn'nnrTG
Cuts at: 1036 2353 2480 1036
Size: 1317 127 2920
Fragments arranged by size:
2920 1317 127
MspA1I CmG'CkG
Cuts at: 1475 1789 2149 3162 3407 1475
Size: 314 360 1013 245 2432
Fragments arranged by size:
2432 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2545 2545
Size: 4364
NarI GG'CG_CC
Cuts at: 1795 2043 1795
Size: 248 4116
NcoI C'CATG_G
Cuts at: 515 515
Size: 4364
NgoMIV G'CCGG_C
Cuts at: 2543 2543
Size: 4364
NspI r_CATG'y
Cuts at: 1417 1917 2824 1417
Size: 500 907 2957
Fragments arranged by size:
2957 907 500
PciI A'CATG_T
Cuts at: 2820 2820
Size: 4364
PflMI CCAn_nnn'nTGG
Cuts at: 859 859
Size: 4364
PmeI GTTT'AAAC
Cuts at: 1363 1363
Size: 4364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 3503
Size: 32 4332
PshAI GACnn'nnGTC
Cuts at: 151 151
Size: 4364
PsiI TTA'TAA
Cuts at: 1241 1241
Size: 4364
PstI C_TGCA'G
Cuts at: 1411 2096 1411
Size: 685 3679
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4364
PvuII CAG'CTG
Cuts at: 1789 2149 1789
Size: 360 4004
RsrII CG'GwC_CG
Cuts at: 2559 2559
Size: 4364
SacI G_AGCT'C
Cuts at: 1378 1378
Size: 4364
SalI G'TCGA_C
Cuts at: 1401 1401
Size: 4364
SapI GCTCTTCn'nnn_
Cuts at: 494 623 929 2387 2597 494
Size: 129 306 1458 210 2261
Fragments arranged by size:
2261 1458 306 210 129
SbfI CC_TGCA'GG
Cuts at: 1411 1411
Size: 4364
ScaI AGT'ACT
Cuts at: 3881 3881
Size: 4364
SfcI C'TryA_G
Cuts at: 33 1407 2092 3085 3276 33
Size: 1374 685 993 191 1121
Fragments arranged by size:
1374 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1796 2044 1796
Size: 248 4116
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
SmaI CCC'GGG
Cuts at: 1386 1386
Size: 4364
SmlI C'TyrA_G
Cuts at: 1519 2434 2926 3188 1519
Size: 915 492 262 2695
Fragments arranged by size:
2695 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4364
SphI G_CATG'C
Cuts at: 1417 1917 1417
Size: 500 3864
SspI AAT'ATT
Cuts at: 442 804 959 1237 442
Size: 362 155 278 3569
Fragments arranged by size:
3569 362 278 155
StuI AGG'CCT
Cuts at: 175 175
Size: 4364
StyI C'CwwG_G
Cuts at: 515 603 882 1498 1852 515
Size: 88 279 616 354 3027
Fragments arranged by size:
3027 616 354 279 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1947 2080 2539 2748 1947
Size: 133 459 209 3563
Fragments arranged by size:
3563 459 209 133
TatI w'GTAC_w
Cuts at: 655 3879 655
Size: 3224 1140
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3881 3913 3881
Size: 32 4332
Tth111I GACn'n_nGTC
Cuts at: 2161 2161
Size: 4364
XbaI T'CTAG_A
Cuts at: 1395 1395
Size: 4364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 957 1859 957
Size: 902 3462
XmaI C'CCGG_G
Cuts at: 1384 1384
Size: 4364
XmnI GAAnn'nnTTC
Cuts at: 507 507
Size: 4364
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI BaeI
BbvCI BfrBI BglI BmgBI BmtI BsaXI BseRI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI