Restriction map for
pF1KB5272
(Circular) (Six-base) MAPSORT of: pF1KB5272.seq Check: 4372 from: 1 to: 3857
pF1KB5272 3857 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 895 895
Size: 3857
Acc65I G'GTAC_C
Cuts at: 873 873
Size: 3857
AclI AA'CG_TT
Cuts at: 3697 3697
Size: 3857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 610 1682 2114 2861 3129 610
Size: 1072 432 747 268 1338
Fragments arranged by size:
1338 1072 747 432 268
AflIII A'CryG_T
Cuts at: 1209 2313 3290 1209
Size: 1104 977 1776
Fragments arranged by size:
1776 1104 977
AgeI A'CCGG_T
Cuts at: 2245 2245
Size: 3857
AlwNI CAG_nnn'CTG
Cuts at: 156 831 1162 2729 156
Size: 675 331 1567 1284
Fragments arranged by size:
1567 1284 675 331
ApaI G_GGCC'C
Cuts at: 231 231
Size: 3857
ApaLI G'TGCA_C
Cuts at: 2627 2627
Size: 3857
ApoI r'AATT_y
Cuts at: 861 3210 3466 861
Size: 2349 256 1252
Fragments arranged by size:
2349 1252 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3857
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
AvaI C'yCGr_G
Cuts at: 230 877 230
Size: 647 3210
BamHI G'GATC_C
Cuts at: 272 882 272
Size: 610 3247
BanI G'GyrC_C
Cuts at: 873 1287 1535 1570 873
Size: 414 248 35 3160
Fragments arranged by size:
3160 414 248 35
BanII G_rGCy'C
Cuts at: 231 871 1901 231
Size: 640 1030 2187
Fragments arranged by size:
2187 1030 640
BbeI G_GCGC'C
Cuts at: 1291 1539 1291
Size: 248 3609
BbvCI CC'TCA_GC
Cuts at: 242 248 242
Size: 6 3851
BciVI GTATCCnnnnn_n'
Cuts at: 1755 2516 3857 1755
Size: 761 1341 1755
Fragments arranged by size:
1755 1341 761
BclI T'GATC_A
Cuts at: 509 1377 509
Size: 868 2989
BglII A'GATC_T
Cuts at: 1372 3409 1372
Size: 2037 1820
BlpI GC'TnA_GC
Cuts at: 191 452 548 969 191
Size: 261 96 421 3079
Fragments arranged by size:
3079 421 261 96
Bme1580I G_kGCm'C
Cuts at: 231 1482 1575 2631 231
Size: 1251 93 1056 1457
Fragments arranged by size:
1457 1251 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1069 1150 1485 3629 1069
Size: 81 335 2144 1297
Fragments arranged by size:
2144 1297 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 273 657 2224 273
Size: 384 1567 1906
Fragments arranged by size:
1906 1567 384
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 412 598 184
Size: 228 186 3443
Fragments arranged by size:
3443 228 186
Bpu10I CC'TnA_GC
Cuts at: 242 248 305 626 242
Size: 6 57 321 3473
Fragments arranged by size:
3473 321 57 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 640 679 1033 1912 2404 2702 595
Size: 45 39 354 879 492 298 1750
Fragments arranged by size:
1750 879 492 354 298 45 39
BsaI GGTCTCn'nnnn_
Cuts at: 700 3561 700
Size: 2861 996
BsaAI yAC'GTr
Cuts at: 1210 1210
Size: 3857
BsaBI GATnn'nnATC
Cuts at: 6 1371 3408 6
Size: 1365 2037 455
Fragments arranged by size:
2037 1365 455
BsaHI Gr'CG_yC
Cuts at: 1288 1536 2238 3734 1288
Size: 248 702 1496 1411
Fragments arranged by size:
1496 1411 702 248
BsaWI w'CCGG_w
Cuts at: 1046 1567 2245 2519 2666 3077 1046
Size: 521 678 274 147 411 1826
Fragments arranged by size:
1826 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 595 598 808 196
Size: 399 3 210 3245
Fragments arranged by size:
3245 399 210 3
BseYI C'CCAG_C
Cuts at: 112 1282 2040 2617 3113 112
Size: 1170 758 577 496 856
Fragments arranged by size:
1170 856 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1399 1445 2653 104
Size: 1295 46 1208 1308
Fragments arranged by size:
1308 1295 1208 46
BsiHKAI G_wGCw'C
Cuts at: 306 800 871 1649 1839 2631 306
Size: 494 71 778 190 792 1532
Fragments arranged by size:
1532 792 778 494 190 71
Bsp1286I G_dGCh'C
Cuts at: 231 306 800 871 1482 1575 1649 1839 1901
Size: 75 494 71 611 93 74 190 62
Cuts at: 1901 2631 231
Size: 730 1457
Fragments arranged by size:
1457 730 611 494 190 93 75 74
71 62
BspEI T'CCGG_A
Cuts at: 3077 3077
Size: 3857
BspHI T'CATG_A
Cuts at: 3033 3033
Size: 3857
BspMI ACCTGCnnnn'nnnn_
Cuts at: 907 1121 1423 1804 907
Size: 214 302 381 2960
Fragments arranged by size:
2960 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2149 2203 3687 3852 50
Size: 2099 54 1484 165 55
Fragments arranged by size:
2099 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 458 1769 458
Size: 1311 2546
BsrFI r'CCGG_y
Cuts at: 1855 2036 2245 1855
Size: 181 209 3467
Fragments arranged by size:
3467 209 181
BsrGI T'GTAC_A
Cuts at: 805 805
Size: 3857
BssSI C'ACGA_G
Cuts at: 2128 2486 2128
Size: 358 3499
BstAPI GCAn_nnn'nTGC
Cuts at: 175 1234 175
Size: 1059 2798
BstBI TT'CG_AA
Cuts at: 2218 2218
Size: 3857
BstYI r'GATC_y
Cuts at: 1 146 272 882 1349 1372 1707 1953 2965
Size: 145 126 610 467 23 335 246 1012
Cuts at: 2965 3051 3063 3409 1
Size: 86 12 346 449
Fragments arranged by size:
1012 610 467 449 346 335 246 145
126 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3857
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1807 1988 2151 1807
Size: 181 163 3513
Fragments arranged by size:
3513 181 163
BtsI GCAGTG_nn'
Cuts at: 320 1100 320
Size: 780 3077
DraI TTT'AAA
Cuts at: 856 856
Size: 3857
DrdI GACnn_nn'nnGTC
Cuts at: 1563 2421 1563
Size: 858 2999
EaeI y'GGCC_r
Cuts at: 317 1442 1616 2007 2034 3379 317
Size: 1125 174 391 27 1345 795
Fragments arranged by size:
1345 1125 795 391 174 27
EagI C'GGCC_G
Cuts at: 1442 1442
Size: 3857
EarI CTCTTCn'nnn_
Cuts at: 284 461 482 1880 2090 284
Size: 177 21 1398 210 2051
Fragments arranged by size:
2051 1398 210 177 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 646 2065 2375 2521 3667 646
Size: 1419 310 146 1146 836
Fragments arranged by size:
1419 1146 836 310 146
EcoICRI GAG'CTC
Cuts at: 869 869
Size: 3857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 412 598 610 1682 2114 2861 3129 184
Size: 228 186 12 1072 432 747 268 912
Fragments arranged by size:
1072 912 747 432 268 228 186 12
EcoO109I rG'GnC_Cy
Cuts at: 996 996
Size: 3857
EcoRI G'AATT_C
Cuts at: 861 861
Size: 3857
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1876 1876
Size: 3857
FspI TGC'GCA
Cuts at: 1638 1638
Size: 3857
HaeII r_GCGC'y
Cuts at: 198 315 1291 1539 2561 3540 198
Size: 117 976 248 1022 979 515
Fragments arranged by size:
1022 979 976 515 248 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1699 1729 1731 1761 1699
Size: 30 2 30 3795
Fragments arranged by size:
3795 30 30 2
HincII GTy'rAC
Cuts at: 896 896
Size: 3857
KasI G'GCGC_C
Cuts at: 1287 1535 1287
Size: 248 3609
KpnI G_GTAC'C
Cuts at: 877 877
Size: 3857
MfeI C'AATT_G
Cuts at: 755 755
Size: 3857
MluI A'CGCG_T
Cuts at: 3290 3290
Size: 3857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 462 2528 2712 462
Size: 2066 184 1607
Fragments arranged by size:
2066 1607 184
MscI TGG'CCA
Cuts at: 319 1618 319
Size: 1299 2558
MslI CAynn'nnrTG
Cuts at: 1846 1973 1846
Size: 127 3730
MspA1I CmG'CkG
Cuts at: 279 968 1282 1642 2655 2900 279
Size: 689 314 360 1013 245 1236
Fragments arranged by size:
1236 1013 689 360 314 245
NaeI GCC'GGC
Cuts at: 2038 2038
Size: 3857
NarI GG'CG_CC
Cuts at: 1288 1536 1288
Size: 248 3609
NcoI C'CATG_G
Cuts at: 107 107
Size: 3857
NgoMIV G'CCGG_C
Cuts at: 2036 2036
Size: 3857
NspI r_CATG'y
Cuts at: 910 1410 2317 910
Size: 500 907 2450
Fragments arranged by size:
2450 907 500
PciI A'CATG_T
Cuts at: 2313 2313
Size: 3857
PfoI T'CCnGG_A
Cuts at: 428 428
Size: 3857
PmeI GTTT'AAAC
Cuts at: 856 856
Size: 3857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 3825
PspOMI G'GGCC_C
Cuts at: 227 227
Size: 3857
PstI C_TGCA'G
Cuts at: 161 904 1589 161
Size: 743 685 2429
Fragments arranged by size:
2429 743 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3857
PvuII CAG'CTG
Cuts at: 279 1282 1642 279
Size: 1003 360 2494
Fragments arranged by size:
2494 1003 360
RsrII CG'GwC_CG
Cuts at: 2052 2052
Size: 3857
SacI G_AGCT'C
Cuts at: 871 871
Size: 3857
SalI G'TCGA_C
Cuts at: 894 894
Size: 3857
SapI GCTCTTCn'nnn_
Cuts at: 284 1880 2090 284
Size: 1596 210 2051
Fragments arranged by size:
2051 1596 210
SbfI CC_TGCA'GG
Cuts at: 904 904
Size: 3857
ScaI AGT'ACT
Cuts at: 640 3374 640
Size: 2734 1123
SfcI C'TryA_G
Cuts at: 33 157 900 1585 2578 2769 33
Size: 124 743 685 993 191 1121
Fragments arranged by size:
1121 993 743 685 191 124
SfoI GGC'GCC
Cuts at: 1289 1537 1289
Size: 248 3609
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
SmaI CCC'GGG
Cuts at: 232 879 232
Size: 647 3210
SmlI C'TyrA_G
Cuts at: 610 619 694 1012 1927 2419 2681 610
Size: 9 75 318 915 492 262 1786
Fragments arranged by size:
1786 915 492 318 262 75 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3857
SphI G_CATG'C
Cuts at: 910 1410 910
Size: 500 3357
StyI C'CwwG_G
Cuts at: 107 991 1345 107
Size: 884 354 2619
Fragments arranged by size:
2619 884 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1440 1573 2032 2241 1440
Size: 133 459 209 3056
Fragments arranged by size:
3056 459 209 133
TatI w'GTAC_w
Cuts at: 638 805 3372 638
Size: 167 2567 1123
Fragments arranged by size:
2567 1123 167
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3374 3406 3374
Size: 32 3825
Tth111I GACn'n_nGTC
Cuts at: 1654 1654
Size: 3857
XbaI T'CTAG_A
Cuts at: 888 888
Size: 3857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1352 1352
Size: 3857
XmaI C'CCGG_G
Cuts at: 230 877 230
Size: 647 3210
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI