Restriction map for
pF1KB5284
(Circular) (Six-base) MAPSORT of: pF1KB5284.seq Check: 8058 from: 1 to: 3680
pF1KB5284 3680 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 718 718
Size: 3680
Acc65I G'GTAC_C
Cuts at: 696 696
Size: 3680
AclI AA'CG_TT
Cuts at: 3520 3520
Size: 3680
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 676 1505 1937 2684 2952 331
Size: 345 829 432 747 268 1059
Fragments arranged by size:
1059 829 747 432 345 268
AflIII A'CryG_T
Cuts at: 1032 2136 3113 1032
Size: 1104 977 1599
Fragments arranged by size:
1599 1104 977
AgeI A'CCGG_T
Cuts at: 2068 2068
Size: 3680
AlwNI CAG_nnn'CTG
Cuts at: 985 2552 985
Size: 1567 2113
ApaLI G'TGCA_C
Cuts at: 2450 2450
Size: 3680
ApoI r'AATT_y
Cuts at: 684 3033 3289 684
Size: 2349 256 1075
Fragments arranged by size:
2349 1075 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3680
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3680
AvaI C'yCGr_G
Cuts at: 285 337 700 285
Size: 52 363 3265
Fragments arranged by size:
3265 363 52
BamHI G'GATC_C
Cuts at: 705 705
Size: 3680
BanI G'GyrC_C
Cuts at: 696 1110 1358 1393 696
Size: 414 248 35 2983
Fragments arranged by size:
2983 414 248 35
BanII G_rGCy'C
Cuts at: 146 443 694 1724 146
Size: 297 251 1030 2102
Fragments arranged by size:
2102 1030 297 251
BbeI G_GCGC'C
Cuts at: 1114 1362 1114
Size: 248 3432
BbvCI CC'TCA_GC
Cuts at: 410 410
Size: 3680
BciVI GTATCCnnnnn_n'
Cuts at: 1578 2339 3680 1578
Size: 761 1341 1578
Fragments arranged by size:
1578 1341 761
BclI T'GATC_A
Cuts at: 1200 1200
Size: 3680
BglI GCCn_nnn'nGGC
Cuts at: 363 363
Size: 3680
BglII A'GATC_T
Cuts at: 1195 3232 1195
Size: 2037 1643
BlpI GC'TnA_GC
Cuts at: 792 792
Size: 3680
Bme1580I G_kGCm'C
Cuts at: 1305 1398 2454 1305
Size: 93 1056 2531
Fragments arranged by size:
2531 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 892 973 1308 3452 892
Size: 81 335 2144 1120
Fragments arranged by size:
2144 1120 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 3680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 654 654
Size: 3680
Bpu10I CC'TnA_GC
Cuts at: 410 530 410
Size: 120 3560
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 1735 2227 2525 856
Size: 879 492 298 2011
Fragments arranged by size:
2011 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 362 3384 362
Size: 3022 658
BsaAI yAC'GTr
Cuts at: 1033 1033
Size: 3680
BsaBI GATnn'nnATC
Cuts at: 6 1194 3231 6
Size: 1188 2037 455
Fragments arranged by size:
2037 1188 455
BsaHI Gr'CG_yC
Cuts at: 1111 1359 2061 3557 1111
Size: 248 702 1496 1234
Fragments arranged by size:
1496 1234 702 248
BsaWI w'CCGG_w
Cuts at: 869 1390 2068 2342 2489 2900 869
Size: 521 678 274 147 411 1649
Fragments arranged by size:
1649 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 427 646 427
Size: 219 3461
BseYI C'CCAG_C
Cuts at: 1105 1863 2440 2936 1105
Size: 758 577 496 1849
Fragments arranged by size:
1849 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1222 1268 2476 104
Size: 1118 46 1208 1308
Fragments arranged by size:
1308 1208 1118 46
BsiHKAI G_wGCw'C
Cuts at: 146 443 694 1472 1662 2454 146
Size: 297 251 778 190 792 1372
Fragments arranged by size:
1372 792 778 297 251 190
Bsp1286I G_dGCh'C
Cuts at: 146 443 694 1305 1398 1472 1662 1724 2454
Size: 297 251 611 93 74 190 62 730
Cuts at: 2454 146
Size: 1372
Fragments arranged by size:
1372 730 611 297 251 190 93 74
62
BspEI T'CCGG_A
Cuts at: 2900 2900
Size: 3680
BspHI T'CATG_A
Cuts at: 2856 2856
Size: 3680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 730 944 1246 1627 730
Size: 214 302 381 2783
Fragments arranged by size:
2783 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1972 2026 3510 3675 50
Size: 1922 54 1484 165 55
Fragments arranged by size:
1922 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1592 1592
Size: 3680
BsrFI r'CCGG_y
Cuts at: 1678 1859 2068 1678
Size: 181 209 3290
Fragments arranged by size:
3290 209 181
BssSI C'ACGA_G
Cuts at: 1951 2309 1951
Size: 358 3322
BstAPI GCAn_nnn'nTGC
Cuts at: 1057 1057
Size: 3680
BstBI TT'CG_AA
Cuts at: 2041 2041
Size: 3680
BstYI r'GATC_y
Cuts at: 1 386 705 1172 1195 1530 1776 2788 2874
Size: 385 319 467 23 335 246 1012 86
Cuts at: 2874 2886 3232 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 385 346 335 319 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1630 1811 1974 1630
Size: 181 163 3336
Fragments arranged by size:
3336 181 163
BtsI GCAGTG_nn'
Cuts at: 923 923
Size: 3680
DraI TTT'AAA
Cuts at: 679 679
Size: 3680
DrdI GACnn_nn'nnGTC
Cuts at: 259 1386 2244 259
Size: 1127 858 1695
Fragments arranged by size:
1695 1127 858
EaeI y'GGCC_r
Cuts at: 179 1265 1439 1830 1857 3202 179
Size: 1086 174 391 27 1345 657
Fragments arranged by size:
1345 1086 657 391 174 27
EagI C'GGCC_G
Cuts at: 1265 1265
Size: 3680
EarI CTCTTCn'nnn_
Cuts at: 377 656 1703 1913 377
Size: 279 1047 210 2144
Fragments arranged by size:
2144 1047 279 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1888 2198 2344 3490 1888
Size: 310 146 1146 2078
Fragments arranged by size:
2078 1146 310 146
EcoICRI GAG'CTC
Cuts at: 144 441 692 144
Size: 297 251 3132
Fragments arranged by size:
3132 297 251
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 654 676 1505 1937 2684 2952 331
Size: 323 22 829 432 747 268 1059
Fragments arranged by size:
1059 829 747 432 323 268 22
EcoO109I rG'GnC_Cy
Cuts at: 819 819
Size: 3680
EcoRI G'AATT_C
Cuts at: 684 684
Size: 3680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1699 1699
Size: 3680
FspI TGC'GCA
Cuts at: 1461 1461
Size: 3680
HaeII r_GCGC'y
Cuts at: 1114 1362 2384 3363 1114
Size: 248 1022 979 1431
Fragments arranged by size:
1431 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 470 502 1522 1552 1554 1584 470
Size: 32 1020 30 2 30 2566
Fragments arranged by size:
2566 1020 32 30 30 2
HincII GTy'rAC
Cuts at: 719 719
Size: 3680
KasI G'GCGC_C
Cuts at: 1110 1358 1110
Size: 248 3432
KpnI G_GTAC'C
Cuts at: 700 700
Size: 3680
MluI A'CGCG_T
Cuts at: 3113 3113
Size: 3680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 275 578 584 2351 2535 275
Size: 303 6 1767 184 1420
Fragments arranged by size:
1767 1420 303 184 6
MscI TGG'CCA
Cuts at: 1441 1441
Size: 3680
MslI CAynn'nnrTG
Cuts at: 523 1669 1796 523
Size: 1146 127 2407
Fragments arranged by size:
2407 1146 127
MspA1I CmG'CkG
Cuts at: 150 791 1105 1465 2478 2723 150
Size: 641 314 360 1013 245 1107
Fragments arranged by size:
1107 1013 641 360 314 245
NaeI GCC'GGC
Cuts at: 1861 1861
Size: 3680
NarI GG'CG_CC
Cuts at: 1111 1359 1111
Size: 248 3432
NgoMIV G'CCGG_C
Cuts at: 1859 1859
Size: 3680
NspI r_CATG'y
Cuts at: 733 1233 2140 733
Size: 500 907 2273
Fragments arranged by size:
2273 907 500
PciI A'CATG_T
Cuts at: 2136 2136
Size: 3680
PflMI CCAn_nnn'nTGG
Cuts at: 552 552
Size: 3680
PmeI GTTT'AAAC
Cuts at: 679 679
Size: 3680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2819 2851 2819
Size: 32 3648
PstI C_TGCA'G
Cuts at: 727 1412 727
Size: 685 2995
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3680
PvuII CAG'CTG
Cuts at: 1105 1465 1105
Size: 360 3320
RsrII CG'GwC_CG
Cuts at: 1875 1875
Size: 3680
SacI G_AGCT'C
Cuts at: 146 443 694 146
Size: 297 251 3132
Fragments arranged by size:
3132 297 251
SalI G'TCGA_C
Cuts at: 717 717
Size: 3680
SapI GCTCTTCn'nnn_
Cuts at: 1703 1913 1703
Size: 210 3470
SbfI CC_TGCA'GG
Cuts at: 727 727
Size: 3680
ScaI AGT'ACT
Cuts at: 3197 3197
Size: 3680
SfcI C'TryA_G
Cuts at: 33 723 1408 2401 2592 33
Size: 690 685 993 191 1121
Fragments arranged by size:
1121 993 690 685 191
SfoI GGC'GCC
Cuts at: 1112 1360 1112
Size: 248 3432
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3680
SmaI CCC'GGG
Cuts at: 702 702
Size: 3680
SmlI C'TyrA_G
Cuts at: 835 1750 2242 2504 835
Size: 915 492 262 2011
Fragments arranged by size:
2011 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3680
SphI G_CATG'C
Cuts at: 733 1233 733
Size: 500 3180
StuI AGG'CCT
Cuts at: 667 667
Size: 3680
StyI C'CwwG_G
Cuts at: 358 814 1168 358
Size: 456 354 2870
Fragments arranged by size:
2870 456 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1263 1396 1855 2064 1263
Size: 133 459 209 2879
Fragments arranged by size:
2879 459 209 133
TatI w'GTAC_w
Cuts at: 3195 3195
Size: 3680
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3197 3229 3197
Size: 32 3648
Tth111I GACn'n_nGTC
Cuts at: 1477 1477
Size: 3680
XbaI T'CTAG_A
Cuts at: 711 711
Size: 3680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1175 1175
Size: 3680
XmaI C'CCGG_G
Cuts at: 700 700
Size: 3680
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BmgBI
BmtI BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI