Restriction map for
pF1KB5288
(Circular) (Six-base) MAPSORT of: pF1KB5288.seq Check: 1348 from: 1 to: 4763
pF1KB5288 4763 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 345 345
Size: 4763
AccI GT'mk_AC
Cuts at: 1801 1801
Size: 4763
Acc65I G'GTAC_C
Cuts at: 1009 1779 1009
Size: 770 3993
AclI AA'CG_TT
Cuts at: 4603 4603
Size: 4763
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 2588 3020 3373 4035 1240
Size: 1348 432 353 662 1968
Fragments arranged by size:
1968 1348 662 432 353
AfeI AGC'GCT
Cuts at: 409 409
Size: 4763
AflIII A'CryG_T
Cuts at: 2115 3915 4196 2115
Size: 1800 281 2682
Fragments arranged by size:
2682 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1265 1265
Size: 4763
AleI CACnn'nnGTG
Cuts at: 459 459
Size: 4763
AlwNI CAG_nnn'CTG
Cuts at: 273 360 1117 2068 3506 273
Size: 87 757 951 1438 1530
Fragments arranged by size:
1530 1438 951 757 87
ApaI G_GGCC'C
Cuts at: 684 684
Size: 4763
ApaLI G'TGCA_C
Cuts at: 3601 3601
Size: 4763
ApoI r'AATT_y
Cuts at: 1767 4116 4372 1767
Size: 2349 256 2158
Fragments arranged by size:
2349 2158 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4763
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4763
AvaI C'yCGr_G
Cuts at: 1160 1783 1160
Size: 623 4140
AvrII C'CTAG_G
Cuts at: 1657 1657
Size: 4763
BamHI G'GATC_C
Cuts at: 1788 1788
Size: 4763
BanI G'GyrC_C
Cuts at: 1009 1779 2193 2441 2476 1009
Size: 770 414 248 35 3296
Fragments arranged by size:
3296 770 414 248 35
BanII G_rGCy'C
Cuts at: 684 1665 1777 2807 684
Size: 981 112 1030 2640
Fragments arranged by size:
2640 1030 981 112
BbeI G_GCGC'C
Cuts at: 2197 2445 2197
Size: 248 4515
BbsI GAAGACnn'nnnn_
Cuts at: 373 373
Size: 4763
BbvCI CC'TCA_GC
Cuts at: 316 617 1688 316
Size: 301 1071 3391
Fragments arranged by size:
3391 1071 301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4729
BciVI GTATCCnnnnn_n'
Cuts at: 2661 3717 4763 2661
Size: 1056 1046 2661
Fragments arranged by size:
2661 1056 1046
BclI T'GATC_A
Cuts at: 2283 2283
Size: 4763
BglI GCCn_nnn'nGGC
Cuts at: 1570 1570
Size: 4763
BglII A'GATC_T
Cuts at: 2278 4315 2278
Size: 2037 2726
BlpI GC'TnA_GC
Cuts at: 404 974 1875 404
Size: 570 901 3292
Fragments arranged by size:
3292 901 570
Bme1580I G_kGCm'C
Cuts at: 684 1053 2388 2481 3605 684
Size: 369 1335 93 1124 1842
Fragments arranged by size:
1842 1335 1124 369 93
BmrI ACTGGGnnnn_n'
Cuts at: 808 1056 1127 1975 2056 2391 4535 808
Size: 248 71 848 81 335 2144 1036
Fragments arranged by size:
2144 1036 848 335 248 81 71
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3130 3130
Size: 4763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 196 295 1516 1729 151
Size: 45 99 1221 213 3185
Fragments arranged by size:
3185 1221 213 99 45
Bpu10I CC'TnA_GC
Cuts at: 316 617 1210 1688 316
Size: 301 593 478 3391
Fragments arranged by size:
3391 593 478 301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1939 2818 3532 3830 1939
Size: 879 714 298 2872
Fragments arranged by size:
2872 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1605 4467 1605
Size: 2862 1901
BsaAI yAC'GTr
Cuts at: 2116 2116
Size: 4763
BsaBI GATnn'nnATC
Cuts at: 6 2277 4314 6
Size: 2271 2037 455
Fragments arranged by size:
2271 2037 455
BsaHI Gr'CG_yC
Cuts at: 2194 2442 3144 4640 2194
Size: 248 702 1496 2317
Fragments arranged by size:
2317 1496 702 248
BsaWI w'CCGG_w
Cuts at: 215 446 1952 2473 3151 3562 3709 3983 215
Size: 231 1506 521 678 411 147 274 995
Fragments arranged by size:
1506 995 678 521 411 274 231 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1285 1315 1356 1386 1285
Size: 30 41 30 4662
Fragments arranged by size:
4662 41 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 729 934 729
Size: 205 4558
BseYI C'CCAG_C
Cuts at: 257 360 506 531 775 1030 1475 1575 2188
Size: 103 146 25 244 255 445 100 613
Cuts at: 2188 2946 3611 4019 257
Size: 758 665 408 1001
Fragments arranged by size:
1001 758 665 613 445 408 255 244
146 103 100 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1042 1042
Size: 4763
BsiEI CG_ry'CG
Cuts at: 104 426 2305 2351 3581 104
Size: 322 1879 46 1230 1286
Fragments arranged by size:
1879 1286 1230 322 46
BsiHKAI G_wGCw'C
Cuts at: 611 1777 2555 2745 3605 611
Size: 1166 778 190 860 1769
Fragments arranged by size:
1769 1166 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 611 684 1053 1665 1777 2388 2481 2555 2745
Size: 73 369 612 112 611 93 74 190
Cuts at: 2745 2807 3605 611
Size: 62 798 1769
Fragments arranged by size:
1769 798 612 611 369 190 112 93
74 73 62
BspEI T'CCGG_A
Cuts at: 215 446 215
Size: 231 4532
BspHI T'CATG_A
Cuts at: 915 1352 3195 915
Size: 437 1843 2483
Fragments arranged by size:
2483 1843 437
BspMI ACCTGCnnnn'nnnn_
Cuts at: 345 1813 2027 2329 2710 345
Size: 1468 214 302 381 2398
Fragments arranged by size:
2398 1468 381 302 214
BsrBI CCG'CTC
Cuts at: 50 737 3055 3109 4593 4758 50
Size: 687 2318 54 1484 165 55
Fragments arranged by size:
2318 1484 687 165 55 54
BsrDI GCAATG_nn'
Cuts at: 609 1526 2675 609
Size: 917 1149 2697
Fragments arranged by size:
2697 1149 917
BsrFI r'CCGG_y
Cuts at: 374 1094 2761 2942 374
Size: 720 1667 181 2195
Fragments arranged by size:
2195 1667 720 181
BssSI C'ACGA_G
Cuts at: 3034 3742 3034
Size: 708 4055
BstAPI GCAn_nnn'nTGC
Cuts at: 2140 2140
Size: 4763
BstBI TT'CG_AA
Cuts at: 3124 3124
Size: 4763
BstXI CCAn_nnnn'nTGG
Cuts at: 461 461
Size: 4763
BstYI r'GATC_y
Cuts at: 1 212 1788 2255 2278 2613 2859 3165 3177
Size: 211 1576 467 23 335 246 306 12
Cuts at: 3177 3263 4315 1
Size: 86 1052 449
Fragments arranged by size:
1576 1052 467 449 335 306 246 211
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 741 1015 1103 741
Size: 274 88 4401
Fragments arranged by size:
4401 274 88
BtgI C'CryG_G
Cuts at: 420 1699 420
Size: 1279 3484
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 746 1434 2713 2894 3057 746
Size: 688 1279 181 163 2452
Fragments arranged by size:
2452 1279 688 181 163
BtsI GCAGTG_nn'
Cuts at: 878 998 1276 1342 2006 878
Size: 120 278 66 664 3635
Fragments arranged by size:
3635 664 278 120 66
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 798 833 798
Size: 35 4728
DraI TTT'AAA
Cuts at: 1762 1762
Size: 4763
DrdI GACnn_nn'nnGTC
Cuts at: 2469 3813 2469
Size: 1344 3419
EaeI y'GGCC_r
Cuts at: 423 2348 2522 2913 2940 4285 423
Size: 1925 174 391 27 1345 901
Fragments arranged by size:
1925 1345 901 391 174 27
EagI C'GGCC_G
Cuts at: 423 2348 423
Size: 1925 2838
EarI CTCTTCn'nnn_
Cuts at: 616 668 1090 1118 2786 2996 616
Size: 52 422 28 1668 210 2383
Fragments arranged by size:
2383 1668 422 210 52 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2971 3713 3859 4573 2971
Size: 742 146 714 3161
Fragments arranged by size:
3161 742 714 146
EcoICRI GAG'CTC
Cuts at: 1775 1775
Size: 4763
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 196 295 1240 1516 1729 2588 3020 3373
Size: 45 99 945 276 213 859 432 353
Cuts at: 3373 4035 151
Size: 662 879
Fragments arranged by size:
945 879 859 662 432 353 276 213
99 45
EcoNI CCTnn'n_nnAGG
Cuts at: 744 744
Size: 4763
EcoO109I rG'GnC_Cy
Cuts at: 135 230 575 659 680 681 1409 1902 135
Size: 95 345 84 21 1 728 493 2996
Fragments arranged by size:
2996 728 493 345 95 84 21 1
EcoRI G'AATT_C
Cuts at: 1767 1767
Size: 4763
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2782 2782
Size: 4763
FspI TGC'GCA
Cuts at: 2544 2544
Size: 4763
HaeII r_GCGC'y
Cuts at: 411 2197 2445 3675 4446 411
Size: 1786 248 1230 771 728
Fragments arranged by size:
1786 1230 771 728 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 110 142 1251 1281 1283 1313 2605 2635 2637
Size: 32 1109 30 2 30 1292 30 2
Cuts at: 2637 2667 110
Size: 30 2206
Fragments arranged by size:
2206 1292 1109 32 30 30 30 30
2 2
HincII GTy'rAC
Cuts at: 1149 1802 1149
Size: 653 4110
KasI G'GCGC_C
Cuts at: 2193 2441 2193
Size: 248 4515
KpnI G_GTAC'C
Cuts at: 1013 1783 1013
Size: 770 3993
MluI A'CGCG_T
Cuts at: 4196 4196
Size: 4763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3522 3706 3522
Size: 184 4579
MscI TGG'CCA
Cuts at: 2524 2524
Size: 4763
MslI CAynn'nnrTG
Cuts at: 459 556 2752 2879 459
Size: 97 2196 127 2343
Fragments arranged by size:
2343 2196 127 97
MspA1I CmG'CkG
Cuts at: 339 403 422 506 531 1131 1440 1456 1479
Size: 64 19 84 25 600 309 16 23
Cuts at: 1479 1874 2188 2548 3332 3577 339
Size: 395 314 360 784 245 1525
Fragments arranged by size:
1525 784 600 395 360 314 309 245
84 64 25 23 19 16
NaeI GCC'GGC
Cuts at: 376 2944 376
Size: 2568 2195
NarI GG'CG_CC
Cuts at: 2194 2442 2194
Size: 248 4515
NgoMIV G'CCGG_C
Cuts at: 374 2942 374
Size: 2568 2195
NspI r_CATG'y
Cuts at: 1816 2316 3919 1816
Size: 500 1603 2660
Fragments arranged by size:
2660 1603 500
PasI CC'CwG_GG
Cuts at: 729 1604 729
Size: 875 3888
PciI A'CATG_T
Cuts at: 3915 3915
Size: 4763
PflMI CCAn_nnn'nTGG
Cuts at: 820 843 1395 1425 820
Size: 23 552 30 4158
Fragments arranged by size:
4158 552 30 23
PfoI T'CCnGG_A
Cuts at: 128 545 128
Size: 417 4346
PmeI GTTT'AAAC
Cuts at: 1762 1762
Size: 4763
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 4731
PpuMI rG'GwC_Cy
Cuts at: 135 575 1409 135
Size: 440 834 3489
Fragments arranged by size:
3489 834 440
PshAI GACnn'nnGTC
Cuts at: 1162 1162
Size: 4763
PspOMI G'GGCC_C
Cuts at: 680 680
Size: 4763
PspXI vC'TCGA_Gb
Cuts at: 1160 1160
Size: 4763
PstI C_TGCA'G
Cuts at: 338 530 1283 1720 1810 2495 338
Size: 192 753 437 90 685 2606
Fragments arranged by size:
2606 753 685 437 192 90
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4763
PvuII CAG'CTG
Cuts at: 531 2188 2548 531
Size: 1657 360 2746
Fragments arranged by size:
2746 1657 360
RsrII CG'GwC_CG
Cuts at: 2958 2958
Size: 4763
SacI G_AGCT'C
Cuts at: 1777 1777
Size: 4763
SacII CC_GC'GG
Cuts at: 423 423
Size: 4763
SalI G'TCGA_C
Cuts at: 1800 1800
Size: 4763
SapI GCTCTTCn'nnn_
Cuts at: 616 1118 2786 2996 616
Size: 502 1668 210 2383
Fragments arranged by size:
2383 1668 502 210
SbfI CC_TGCA'GG
Cuts at: 1810 1810
Size: 4763
ScaI AGT'ACT
Cuts at: 4280 4280
Size: 4763
SfcI C'TryA_G
Cuts at: 33 334 526 1279 1716 1806 2491 3459 3650
Size: 301 192 753 437 90 685 968 191
Cuts at: 3650 33
Size: 1146
Fragments arranged by size:
1146 968 753 685 437 301 192 191
90
SfoI GGC'GCC
Cuts at: 2195 2443 2195
Size: 248 4515
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4763
SmaI CCC'GGG
Cuts at: 1785 1785
Size: 4763
SmlI C'TyrA_G
Cuts at: 1160 1918 2833 3547 3809 1160
Size: 758 915 714 262 2114
Fragments arranged by size:
2114 915 758 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4763
SphI G_CATG'C
Cuts at: 1816 2316 1816
Size: 500 4263
SspI AAT'ATT
Cuts at: 478 478
Size: 4763
StuI AGG'CCT
Cuts at: 1712 1712
Size: 4763
StyI C'CwwG_G
Cuts at: 1657 1897 2251 1657
Size: 240 354 4169
Fragments arranged by size:
4169 354 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2346 2479 2938 3147 2346
Size: 133 459 209 3962
Fragments arranged by size:
3962 459 209 133
TatI w'GTAC_w
Cuts at: 4278 4278
Size: 4763
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1285 1317 4280 4312 1285
Size: 32 2963 32 1736
Fragments arranged by size:
2963 1736 32 32
Tth111I GACn'n_nGTC
Cuts at: 2560 2560
Size: 4763
XbaI T'CTAG_A
Cuts at: 1794 1794
Size: 4763
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 861 1583 2258 861
Size: 722 675 3366
Fragments arranged by size:
3366 722 675
XhoI C'TCGA_G
Cuts at: 1160 1160
Size: 4763
XmaI C'CCGG_G
Cuts at: 1783 1783
Size: 4763
XmnI GAAnn'nnTTC
Cuts at: 1623 1623
Size: 4763
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AgeI AlfI AloI AscI BaeI BfrBI
BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PmlI PsiI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI ZraI