Restriction map for
pF1KB5292
(Circular) (Six-base) MAPSORT of: pF1KB5292.seq Check: 5769 from: 1 to: 4349
pF1KB5292 4349 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AatII G_ACGT'C
Cuts at: 1271 1271
Size: 4349
AccI GT'mk_AC
Cuts at: 1387 1387
Size: 4349
Acc65I G'GTAC_C
Cuts at: 511 554 1365 511
Size: 43 811 3495
Fragments arranged by size:
3495 811 43
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1147 2174 2606 3353 3621 1147
Size: 1027 432 747 268 1875
Fragments arranged by size:
1875 1027 747 432 268
AflIII A'CryG_T
Cuts at: 1701 2805 3782 1701
Size: 1104 977 2268
Fragments arranged by size:
2268 1104 977
AgeI A'CCGG_T
Cuts at: 2737 2737
Size: 4349
AhdI GACnn_n'nnGTC
Cuts at: 573 573
Size: 4349
AleI CACnn'nnGTG
Cuts at: 499 499
Size: 4349
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 778 810 778
Size: 32 4317
AlwNI CAG_nnn'CTG
Cuts at: 607 774 1654 3221 607
Size: 167 880 1567 1735
Fragments arranged by size:
1735 1567 880 167
ApaI G_GGCC'C
Cuts at: 305 1018 305
Size: 713 3636
ApaLI G'TGCA_C
Cuts at: 3119 3119
Size: 4349
ApoI r'AATT_y
Cuts at: 1353 3702 3958 1353
Size: 2349 256 1744
Fragments arranged by size:
2349 1744 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 310 537 1369 310
Size: 227 832 3290
Fragments arranged by size:
3290 832 227
BamHI G'GATC_C
Cuts at: 176 461 1256 1374 176
Size: 285 795 118 3151
Fragments arranged by size:
3151 795 285 118
BanI G'GyrC_C
Cuts at: 282 511 554 579 1365 1779 2027 2062 282
Size: 229 43 25 786 414 248 35 2569
Fragments arranged by size:
2569 786 414 248 229 43 35 25
BanII G_rGCy'C
Cuts at: 232 305 406 1018 1363 2393 232
Size: 73 101 612 345 1030 2188
Fragments arranged by size:
2188 1030 612 345 101 73
BbeI G_GCGC'C
Cuts at: 1783 2031 1783
Size: 248 4101
BbsI GAAGACnn'nnnn_
Cuts at: 384 741 1201 1263 384
Size: 357 460 62 3470
Fragments arranged by size:
3470 460 357 62
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4315
BciVI GTATCCnnnnn_n'
Cuts at: 2247 3008 4349 2247
Size: 761 1341 2247
Fragments arranged by size:
2247 1341 761
BclI T'GATC_A
Cuts at: 1869 1869
Size: 4349
BglI GCCn_nnn'nGGC
Cuts at: 1054 1211 1054
Size: 157 4192
BglII A'GATC_T
Cuts at: 1864 3901 1864
Size: 2037 2312
BlpI GC'TnA_GC
Cuts at: 1461 1461
Size: 4349
Bme1580I G_kGCm'C
Cuts at: 305 584 1018 1974 2067 3123 305
Size: 279 434 956 93 1056 1531
Fragments arranged by size:
1531 1056 956 434 279 93
BmgBI CAC'GTC
Cuts at: 197 197
Size: 4349
BmrI ACTGGGnnnn_n'
Cuts at: 333 369 1561 1642 1977 4121 333
Size: 36 1192 81 335 2144 561
Fragments arranged by size:
2144 1192 561 335 81 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 4349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 102
Size: 4349
Bpu10I CC'TnA_GC
Cuts at: 559 805 1207 559
Size: 246 402 3701
Fragments arranged by size:
3701 402 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 1159 1525 2404 2896 3194 304
Size: 855 366 879 492 298 1459
Fragments arranged by size:
1459 879 855 492 366 298
BsaI GGTCTCn'nnnn_
Cuts at: 4053 4053
Size: 4349
BsaAI yAC'GTr
Cuts at: 501 1702 501
Size: 1201 3148
BsaBI GATnn'nnATC
Cuts at: 6 358 1863 3900 6
Size: 352 1505 2037 455
Fragments arranged by size:
2037 1505 455 352
BsaHI Gr'CG_yC
Cuts at: 335 367 1268 1780 2028 2730 4226 335
Size: 32 901 512 248 702 1496 458
Fragments arranged by size:
1496 901 702 512 458 248 32
BsaWI w'CCGG_w
Cuts at: 1538 2059 2737 3011 3158 3569 1538
Size: 521 678 274 147 411 2318
Fragments arranged by size:
2318 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 388 418 778 808 388
Size: 30 360 30 3929
Fragments arranged by size:
3929 360 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 396 396
Size: 4349
BseYI C'CCAG_C
Cuts at: 846 1131 1240 1774 2532 3109 3605 846
Size: 285 109 534 758 577 496 1590
Fragments arranged by size:
1590 758 577 534 496 285 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 169 137
Size: 32 4317
BsiEI CG_ry'CG
Cuts at: 104 1281 1891 1937 3145 104
Size: 1177 610 46 1208 1308
Fragments arranged by size:
1308 1208 1177 610 46
BsiHKAI G_wGCw'C
Cuts at: 320 1363 2141 2331 3123 320
Size: 1043 778 190 792 1546
Fragments arranged by size:
1546 1043 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 267 267
Size: 4349
Bsp1286I G_dGCh'C
Cuts at: 232 305 320 406 584 1018 1363 1974 2067
Size: 73 15 86 178 434 345 611 93
Cuts at: 2067 2141 2331 2393 3123 232
Size: 74 190 62 730 1458
Fragments arranged by size:
1458 730 611 434 345 190 178 93
86 74 73 62 15
BspEI T'CCGG_A
Cuts at: 3569 3569
Size: 4349
BspHI T'CATG_A
Cuts at: 3525 3525
Size: 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 627 909 1399 1613 1915 2296 627
Size: 282 490 214 302 381 2680
Fragments arranged by size:
2680 490 381 302 282 214
BsrBI CCG'CTC
Cuts at: 50 132 1211 2641 2695 4179 4344 50
Size: 82 1079 1430 54 1484 165 55
Fragments arranged by size:
1484 1430 1079 165 82 55 54
BsrDI GCAATG_nn'
Cuts at: 2261 2261
Size: 4349
BsrFI r'CCGG_y
Cuts at: 2347 2528 2737 2347
Size: 181 209 3959
Fragments arranged by size:
3959 209 181
BssSI C'ACGA_G
Cuts at: 2620 2978 2620
Size: 358 3991
BstAPI GCAn_nnn'nTGC
Cuts at: 1726 1726
Size: 4349
BstBI TT'CG_AA
Cuts at: 2710 2710
Size: 4349
BstYI r'GATC_y
Cuts at: 1 176 461 1256 1374 1841 1864 2199 2445
Size: 175 285 795 118 467 23 335 246
Cuts at: 2445 3457 3543 3555 3901 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 795 467 449 346 335 285 246
175 118 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 435 663 2299 2480 2643 435
Size: 228 1636 181 163 2141
Fragments arranged by size:
2141 1636 228 181 163
BtsI GCAGTG_nn'
Cuts at: 432 1592 432
Size: 1160 3189
DraI TTT'AAA
Cuts at: 1348 1348
Size: 4349
DrdI GACnn_nn'nnGTC
Cuts at: 2055 2913 2055
Size: 858 3491
EaeI y'GGCC_r
Cuts at: 944 1278 1934 2108 2499 2526 3871 944
Size: 334 656 174 391 27 1345 1422
Fragments arranged by size:
1422 1345 656 391 334 174 27
EagI C'GGCC_G
Cuts at: 1278 1934 1278
Size: 656 3693
EarI CTCTTCn'nnn_
Cuts at: 2372 2582 2372
Size: 210 4139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 193 2557 2867 3013 4159 193
Size: 2364 310 146 1146 383
Fragments arranged by size:
2364 1146 383 310 146
EcoICRI GAG'CTC
Cuts at: 1361 1361
Size: 4349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 1147 2174 2606 3353 3621 102
Size: 1045 1027 432 747 268 830
Fragments arranged by size:
1045 1027 830 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 562 931 964 562
Size: 369 33 3947
Fragments arranged by size:
3947 369 33
EcoO109I rG'GnC_Cy
Cuts at: 240 256 301 420 813 1488 240
Size: 16 45 119 393 675 3101
Fragments arranged by size:
3101 675 393 119 45 16
EcoRI G'AATT_C
Cuts at: 1353 1353
Size: 4349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 4349
FspI TGC'GCA
Cuts at: 824 2130 824
Size: 1306 3043
HaeII r_GCGC'y
Cuts at: 1783 2031 3053 4032 1783
Size: 248 1022 979 2100
Fragments arranged by size:
2100 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 388 420 559 591 1087 1119 2191 2221 2223
Size: 32 139 32 496 32 1072 30 2
Cuts at: 2223 2253 388
Size: 30 2484
Fragments arranged by size:
2484 1072 496 139 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 732 1388 732
Size: 656 3693
KasI G'GCGC_C
Cuts at: 1779 2027 1779
Size: 248 4101
KpnI G_GTAC'C
Cuts at: 515 558 1369 515
Size: 43 811 3495
Fragments arranged by size:
3495 811 43
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 197 434 3020 3204 197
Size: 237 2586 184 1342
Fragments arranged by size:
2586 1342 237 184
MscI TGG'CCA
Cuts at: 946 2110 946
Size: 1164 3185
MslI CAynn'nnrTG
Cuts at: 499 1222 2338 2465 499
Size: 723 1116 127 2383
Fragments arranged by size:
2383 1116 723 127
MspA1I CmG'CkG
Cuts at: 183 950 1065 1244 1460 1774 2134 3147 3392
Size: 767 115 179 216 314 360 1013 245
Cuts at: 3392 183
Size: 1140
Fragments arranged by size:
1140 1013 767 360 314 245 216 179
115
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 4349
NarI GG'CG_CC
Cuts at: 1780 2028 1780
Size: 248 4101
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 4349
NspI r_CATG'y
Cuts at: 1402 1902 2809 1402
Size: 500 907 2942
Fragments arranged by size:
2942 907 500
PasI CC'CwG_GG
Cuts at: 612 612
Size: 4349
PciI A'CATG_T
Cuts at: 2805 2805
Size: 4349
PflMI CCAn_nnn'nTGG
Cuts at: 416 416
Size: 4349
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 778 810 3488 3520 778
Size: 32 2678 32 1607
Fragments arranged by size:
2678 1607 32 32
PpuMI rG'GwC_Cy
Cuts at: 240 420 813 240
Size: 180 393 3776
Fragments arranged by size:
3776 393 180
PspOMI G'GGCC_C
Cuts at: 301 1014 301
Size: 713 3636
PstI C_TGCA'G
Cuts at: 1043 1396 2081 1043
Size: 353 685 3311
Fragments arranged by size:
3311 685 353
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 1774 2134 1774
Size: 360 3989
RsrII CG'GwC_CG
Cuts at: 2544 2544
Size: 4349
SacI G_AGCT'C
Cuts at: 1363 1363
Size: 4349
SalI G'TCGA_C
Cuts at: 1386 1386
Size: 4349
SanDI GG'GwC_CC
Cuts at: 420 813 420
Size: 393 3956
SapI GCTCTTCn'nnn_
Cuts at: 2372 2582 2372
Size: 210 4139
SbfI CC_TGCA'GG
Cuts at: 1396 1396
Size: 4349
ScaI AGT'ACT
Cuts at: 271 343 3866 271
Size: 72 3523 754
Fragments arranged by size:
3523 754 72
SexAI A'CCwGG_T
Cuts at: 932 932
Size: 4349
SfcI C'TryA_G
Cuts at: 33 1039 1392 2077 3070 3261 33
Size: 1006 353 685 993 191 1121
Fragments arranged by size:
1121 1006 993 685 353 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1054 1054
Size: 4349
SfoI GGC'GCC
Cuts at: 1781 2029 1781
Size: 248 4101
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SmaI CCC'GGG
Cuts at: 1371 1371
Size: 4349
SmlI C'TyrA_G
Cuts at: 319 1174 1504 2419 2911 3173 319
Size: 855 330 915 492 262 1495
Fragments arranged by size:
1495 915 855 492 330 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 1402 1902 1402
Size: 500 3849
StuI AGG'CCT
Cuts at: 910 910
Size: 4349
StyI C'CwwG_G
Cuts at: 1483 1837 1483
Size: 354 3995
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 310 838 1254 1932 2065 2524 2733 310
Size: 528 416 678 133 459 209 1926
Fragments arranged by size:
1926 678 528 459 416 209 133
TatI w'GTAC_w
Cuts at: 269 341 473 1079 3864 269
Size: 72 132 606 2785 754
Fragments arranged by size:
2785 754 606 132 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1241 1273 3866 3898 1241
Size: 32 2593 32 1692
Fragments arranged by size:
2593 1692 32 32
Tth111I GACn'n_nGTC
Cuts at: 213 2146 213
Size: 1933 2416
XbaI T'CTAG_A
Cuts at: 1380 1380
Size: 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1844 1844
Size: 4349
XmaI C'CCGG_G
Cuts at: 1369 1369
Size: 4349
XmnI GAAnn'nnTTC
Cuts at: 790 790
Size: 4349
ZraI GAC'GTC
Cuts at: 1269 1269
Size: 4349
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AlfI AscI AvrII BaeI BbvCI
BfrBI BmtI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SgrAI SnaBI SrfI SspI SwaI XhoI