Restriction map for
pF1KB5299
(Circular) (Six-base) MAPSORT of: pF1KB5299.seq Check: 1510 from: 1 to: 4469
pF1KB5299 4469 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 275 275
Size: 4469
AccI GT'mk_AC
Cuts at: 1122 1507 1122
Size: 385 4084
Acc65I G'GTAC_C
Cuts at: 1485 1485
Size: 4469
AclI AA'CG_TT
Cuts at: 4309 4309
Size: 4469
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 490 1103 2294 2726 3079 3741 442
Size: 48 613 1191 432 353 662 1170
Fragments arranged by size:
1191 1170 662 613 432 353 48
AfeI AGC'GCT
Cuts at: 227 227
Size: 4469
AflIII A'CryG_T
Cuts at: 897 1821 3621 3902 897
Size: 924 1800 281 1464
Fragments arranged by size:
1800 1464 924 281
AleI CACnn'nnGTG
Cuts at: 898 898
Size: 4469
AlwNI CAG_nnn'CTG
Cuts at: 1431 1774 3212 1431
Size: 343 1438 2688
Fragments arranged by size:
2688 1438 343
ApaLI G'TGCA_C
Cuts at: 613 3307 613
Size: 2694 1775
ApoI r'AATT_y
Cuts at: 149 1473 3822 4078 149
Size: 1324 2349 256 540
Fragments arranged by size:
2349 1324 540 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4469
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
AvaI C'yCGr_G
Cuts at: 221 694 1489 221
Size: 473 795 3201
Fragments arranged by size:
3201 795 473
BamHI G'GATC_C
Cuts at: 1221 1494 1221
Size: 273 4196
BanI G'GyrC_C
Cuts at: 1485 1899 2147 2182 1485
Size: 414 248 35 3772
Fragments arranged by size:
3772 414 248 35
BanII G_rGCy'C
Cuts at: 175 762 1299 1483 2513 175
Size: 587 537 184 1030 2131
Fragments arranged by size:
2131 1030 587 537 184
BbeI G_GCGC'C
Cuts at: 1903 2151 1903
Size: 248 4221
BbsI GAAGACnn'nnnn_
Cuts at: 248 1043 248
Size: 795 3674
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 237 271 237
Size: 34 4435
BciVI GTATCCnnnnn_n'
Cuts at: 2367 3423 4469 2367
Size: 1056 1046 2367
Fragments arranged by size:
2367 1056 1046
BclI T'GATC_A
Cuts at: 1989 1989
Size: 4469
BglII A'GATC_T
Cuts at: 1984 4021 1984
Size: 2037 2432
BlpI GC'TnA_GC
Cuts at: 1581 1581
Size: 4469
Bme1580I G_kGCm'C
Cuts at: 617 2094 2187 3311 617
Size: 1477 93 1124 1775
Fragments arranged by size:
1775 1477 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 169 303 1681 1762 2097 4241 169
Size: 134 1378 81 335 2144 397
Fragments arranged by size:
2144 1378 397 335 134 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 261 500 2836 261
Size: 239 2336 1894
Fragments arranged by size:
2336 1894 239
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 309 636 1429 149
Size: 160 327 793 3189
Fragments arranged by size:
3189 793 327 160
Bpu10I CC'TnA_GC
Cuts at: 229 761 229
Size: 532 3937
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 1645 2524 3238 3536 301
Size: 1344 879 714 298 1234
Fragments arranged by size:
1344 1234 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1071 4173 1071
Size: 3102 1367
BsaAI yAC'GTr
Cuts at: 1822 1822
Size: 4469
BsaBI GATnn'nnATC
Cuts at: 6 1983 4020 6
Size: 1977 2037 455
Fragments arranged by size:
2037 1977 455
BsaHI Gr'CG_yC
Cuts at: 199 1900 2148 2850 4346 199
Size: 1701 248 702 1496 322
Fragments arranged by size:
1701 1496 702 322 248
BsaWI w'CCGG_w
Cuts at: 710 1211 1658 2179 2857 3268 3415 3689 710
Size: 501 447 521 678 411 147 274 1490
Fragments arranged by size:
1490 678 521 501 447 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 416 416
Size: 4469
BseYI C'CCAG_C
Cuts at: 843 916 1894 2652 3317 3725 843
Size: 73 978 758 665 408 1587
Fragments arranged by size:
1587 978 758 665 408 73
BsiEI CG_ry'CG
Cuts at: 104 2011 2057 3287 104
Size: 1907 46 1230 1286
Fragments arranged by size:
1907 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 446 617 762 895 1091 1415 1483 2261 2451
Size: 171 145 133 196 324 68 778 190
Cuts at: 2451 3311 446
Size: 860 1604
Fragments arranged by size:
1604 860 778 324 196 190 171 145
133 68
BsmI GAATG_Cn'
Cuts at: 1465 1465
Size: 4469
Bsp1286I G_dGCh'C
Cuts at: 175 446 617 762 895 1091 1299 1415 1483
Size: 271 171 145 133 196 208 116 68
Cuts at: 1483 2094 2187 2261 2451 2513 3311 175
Size: 611 93 74 190 62 798 1333
Fragments arranged by size:
1333 798 611 271 208 196 190 171
145 133 116 93 74 68 62
BspEI T'CCGG_A
Cuts at: 1211 1211
Size: 4469
BspHI T'CATG_A
Cuts at: 1385 2901 1385
Size: 1516 2953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 275 1519 1733 2035 2416 275
Size: 1244 214 302 381 2328
Fragments arranged by size:
2328 1244 381 302 214
BsrBI CCG'CTC
Cuts at: 50 650 2761 2815 4299 4464 50
Size: 600 2111 54 1484 165 55
Fragments arranged by size:
2111 1484 600 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2381 2381
Size: 4469
BsrFI r'CCGG_y
Cuts at: 677 1163 2467 2648 677
Size: 486 1304 181 2498
Fragments arranged by size:
2498 1304 486 181
BssSI C'ACGA_G
Cuts at: 2740 3448 2740
Size: 708 3761
BstAPI GCAn_nnn'nTGC
Cuts at: 1095 1846 1095
Size: 751 3718
BstBI TT'CG_AA
Cuts at: 2830 2830
Size: 4469
BstEII G'GTnAC_C
Cuts at: 656 656
Size: 4469
BstXI CCAn_nnnn'nTGG
Cuts at: 287 1233 287
Size: 946 3523
BstYI r'GATC_y
Cuts at: 1 1221 1494 1961 1984 2319 2565 2871 2883
Size: 1220 273 467 23 335 246 306 12
Cuts at: 2883 2969 4021 1
Size: 86 1052 449
Fragments arranged by size:
1220 1052 467 449 335 306 273 246
86 23 12
BtgI C'CryG_G
Cuts at: 602 824 959 602
Size: 222 135 4112
Fragments arranged by size:
4112 222 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2419 2600 2763 2419
Size: 181 163 4125
Fragments arranged by size:
4125 181 163
BtsI GCAGTG_nn'
Cuts at: 837 1712 837
Size: 875 3594
ClaI AT'CG_AT
Cuts at: 420 420
Size: 4469
DraI TTT'AAA
Cuts at: 1468 1468
Size: 4469
DraIII CAC_nnn'GTG
Cuts at: 960 960
Size: 4469
DrdI GACnn_nn'nnGTC
Cuts at: 2175 3519 2175
Size: 1344 3125
EaeI y'GGCC_r
Cuts at: 1392 2054 2228 2619 2646 3991 1392
Size: 662 174 391 27 1345 1870
Fragments arranged by size:
1870 1345 662 391 174 27
EagI C'GGCC_G
Cuts at: 2054 2054
Size: 4469
EarI CTCTTCn'nnn_
Cuts at: 1079 2492 2702 1079
Size: 1413 210 2846
Fragments arranged by size:
2846 1413 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 241 724 2677 3419 3565 4279 241
Size: 483 1953 742 146 714 431
Fragments arranged by size:
1953 742 714 483 431 146
EcoICRI GAG'CTC
Cuts at: 760 1481 760
Size: 721 3748
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 309 442 490 636 1103 1429 2294 2726
Size: 160 133 48 146 467 326 865 432
Cuts at: 2726 3079 3741 149
Size: 353 662 877
Fragments arranged by size:
877 865 662 467 432 353 326 160
146 133 48
EcoNI CCTnn'n_nnAGG
Cuts at: 431 431
Size: 4469
EcoO109I rG'GnC_Cy
Cuts at: 587 766 1608 587
Size: 179 842 3448
Fragments arranged by size:
3448 842 179
EcoRI G'AATT_C
Cuts at: 1473 1473
Size: 4469
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 4469
FspI TGC'GCA
Cuts at: 263 2250 263
Size: 1987 2482
HaeII r_GCGC'y
Cuts at: 229 1903 2151 3381 4152 229
Size: 1674 248 1230 771 546
Fragments arranged by size:
1674 1230 771 546 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 108 140 1183 1206 1215 1238 1273 1305 2311
Size: 32 1043 23 9 23 35 32 1006
Cuts at: 2311 2341 2343 2373 108
Size: 30 2 30 2204
Fragments arranged by size:
2204 1043 1006 35 32 32 30 30
23 23 9 2
HincII GTy'rAC
Cuts at: 1508 1508
Size: 4469
KasI G'GCGC_C
Cuts at: 1899 2147 1899
Size: 248 4221
KpnI G_GTAC'C
Cuts at: 1489 1489
Size: 4469
MfeI C'AATT_G
Cuts at: 350 350
Size: 4469
MluI A'CGCG_T
Cuts at: 897 3902 897
Size: 3005 1464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3228 3412 3228
Size: 184 4285
MscI TGG'CCA
Cuts at: 2230 2230
Size: 4469
MslI CAynn'nnrTG
Cuts at: 434 898 2458 2585 434
Size: 464 1560 127 2318
Fragments arranged by size:
2318 1560 464 127
MspA1I CmG'CkG
Cuts at: 702 909 1580 1894 2254 3038 3283 702
Size: 207 671 314 360 784 245 1888
Fragments arranged by size:
1888 784 671 360 314 245 207
NaeI GCC'GGC
Cuts at: 2650 2650
Size: 4469
NarI GG'CG_CC
Cuts at: 1900 2148 1900
Size: 248 4221
NcoI C'CATG_G
Cuts at: 824 824
Size: 4469
NgoMIV G'CCGG_C
Cuts at: 2648 2648
Size: 4469
NspI r_CATG'y
Cuts at: 1522 2022 3625 1522
Size: 500 1603 2366
Fragments arranged by size:
2366 1603 500
PciI A'CATG_T
Cuts at: 3621 3621
Size: 4469
PflMI CCAn_nnn'nTGG
Cuts at: 1062 1431 1062
Size: 369 4100
PmeI GTTT'AAAC
Cuts at: 1468 1468
Size: 4469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2915 2947 2915
Size: 32 4437
PpuMI rG'GwC_Cy
Cuts at: 587 766 587
Size: 179 4290
PspXI vC'TCGA_Gb
Cuts at: 221 221
Size: 4469
PstI C_TGCA'G
Cuts at: 1516 2201 1516
Size: 685 3784
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4469
PvuII CAG'CTG
Cuts at: 909 1894 2254 909
Size: 985 360 3124
Fragments arranged by size:
3124 985 360
RsrII CG'GwC_CG
Cuts at: 2664 2664
Size: 4469
SacI G_AGCT'C
Cuts at: 762 1483 762
Size: 721 3748
SalI G'TCGA_C
Cuts at: 1506 1506
Size: 4469
SanDI GG'GwC_CC
Cuts at: 766 766
Size: 4469
SapI GCTCTTCn'nnn_
Cuts at: 1079 2492 2702 1079
Size: 1413 210 2846
Fragments arranged by size:
2846 1413 210
SbfI CC_TGCA'GG
Cuts at: 1516 1516
Size: 4469
ScaI AGT'ACT
Cuts at: 3986 3986
Size: 4469
SfcI C'TryA_G
Cuts at: 33 1119 1512 2197 3165 3356 33
Size: 1086 393 685 968 191 1146
Fragments arranged by size:
1146 1086 968 685 393 191
SfoI GGC'GCC
Cuts at: 1901 2149 1901
Size: 248 4221
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
SmaI CCC'GGG
Cuts at: 1491 1491
Size: 4469
SmlI C'TyrA_G
Cuts at: 221 316 1624 2539 3253 3515 221
Size: 95 1308 915 714 262 1175
Fragments arranged by size:
1308 1175 915 714 262 95
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4469
SphI G_CATG'C
Cuts at: 1522 2022 1522
Size: 500 3969
SspI AAT'ATT
Cuts at: 1208 1208
Size: 4469
StyI C'CwwG_G
Cuts at: 824 1603 1957 824
Size: 779 354 3336
Fragments arranged by size:
3336 779 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2052 2185 2644 2853 2052
Size: 133 459 209 3668
Fragments arranged by size:
3668 459 209 133
TatI w'GTAC_w
Cuts at: 3984 3984
Size: 4469
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 491 523 784 816 887 919 1405 1437 3986
Size: 32 261 32 71 32 486 32 2549
Cuts at: 3986 4018 491
Size: 32 942
Fragments arranged by size:
2549 942 486 261 71 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2266 2266
Size: 4469
XbaI T'CTAG_A
Cuts at: 1500 1500
Size: 4469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 600 1964 600
Size: 1364 3105
XhoI C'TCGA_G
Cuts at: 221 221
Size: 4469
XmaI C'CCGG_G
Cuts at: 1489 1489
Size: 4469
XmnI GAAnn'nnTTC
Cuts at: 455 455
Size: 4469
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I CspCI
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspOMI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI ZraI