Restriction map for
pF1KB5300
(Circular) (Six-base) MAPSORT of: pF1KB5300.seq Check: 806 from: 1 to: 4364
pF1KB5300 4364 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 176 1402 176
Size: 1226 3138
Acc65I G'GTAC_C
Cuts at: 1380 1380
Size: 4364
AclI AA'CG_TT
Cuts at: 4204 4204
Size: 4364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2189 2621 3368 3636 2189
Size: 432 747 268 2917
Fragments arranged by size:
2917 747 432 268
AflII C'TTAA_G
Cuts at: 841 841
Size: 4364
AflIII A'CryG_T
Cuts at: 1716 2820 3797 1716
Size: 1104 977 2283
Fragments arranged by size:
2283 1104 977
AgeI A'CCGG_T
Cuts at: 2752 2752
Size: 4364
AhdI GACnn_n'nnGTC
Cuts at: 915 915
Size: 4364
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 373 405 373
Size: 32 4332
AlwNI CAG_nnn'CTG
Cuts at: 352 445 1669 3236 352
Size: 93 1224 1567 1480
Fragments arranged by size:
1567 1480 1224 93
ApaLI G'TGCA_C
Cuts at: 3134 3134
Size: 4364
ApoI r'AATT_y
Cuts at: 250 1368 3717 3973 250
Size: 1118 2349 256 641
Fragments arranged by size:
2349 1118 641 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4364
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
AvaI C'yCGr_G
Cuts at: 1094 1154 1384 1094
Size: 60 230 4074
Fragments arranged by size:
4074 230 60
AvrII C'CTAG_G
Cuts at: 297 297
Size: 4364
BamHI G'GATC_C
Cuts at: 1389 1389
Size: 4364
BanI G'GyrC_C
Cuts at: 1297 1380 1794 2042 2077 1297
Size: 83 414 248 35 3584
Fragments arranged by size:
3584 414 248 83 35
BanII G_rGCy'C
Cuts at: 624 718 1313 1378 2408 624
Size: 94 595 65 1030 2580
Fragments arranged by size:
2580 1030 595 94 65
BbeI G_GCGC'C
Cuts at: 1798 2046 1798
Size: 248 4116
BbvCI CC'TCA_GC
Cuts at: 1082 1082
Size: 4364
BciVI GTATCCnnnnn_n'
Cuts at: 2262 3023 4364 2262
Size: 761 1341 2262
Fragments arranged by size:
2262 1341 761
BclI T'GATC_A
Cuts at: 1884 1884
Size: 4364
BglI GCCn_nnn'nGGC
Cuts at: 299 1015 299
Size: 716 3648
BglII A'GATC_T
Cuts at: 1879 3916 1879
Size: 2037 2327
BlpI GC'TnA_GC
Cuts at: 1100 1476 1100
Size: 376 3988
Bme1580I G_kGCm'C
Cuts at: 320 1989 2082 3138 320
Size: 1669 93 1056 1546
Fragments arranged by size:
1669 1546 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1298 1576 1657 1992 4136 1298
Size: 278 81 335 2144 1526
Fragments arranged by size:
2144 1526 335 278 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 4364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 484
Size: 4364
Bpu10I CC'TnA_GC
Cuts at: 1082 1082
Size: 4364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1540 2419 2911 3209 1540
Size: 879 492 298 2695
Fragments arranged by size:
2695 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4068 4068
Size: 4364
BsaAI yAC'GTr
Cuts at: 1717 1717
Size: 4364
BsaBI GATnn'nnATC
Cuts at: 6 1878 3915 6
Size: 1872 2037 455
Fragments arranged by size:
2037 1872 455
BsaHI Gr'CG_yC
Cuts at: 1795 2043 2745 4241 1795
Size: 248 702 1496 1918
Fragments arranged by size:
1918 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1553 2074 2752 3026 3173 3584 1553
Size: 521 678 274 147 411 2333
Fragments arranged by size:
2333 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 319 349 740 770 319
Size: 30 391 30 3913
Fragments arranged by size:
3913 391 30 30
BseYI C'CCAG_C
Cuts at: 372 438 445 1789 2547 3124 3620 372
Size: 66 7 1344 758 577 496 1116
Fragments arranged by size:
1344 1116 758 577 496 66 7
BsiEI CG_ry'CG
Cuts at: 104 1906 1952 3160 104
Size: 1802 46 1208 1308
Fragments arranged by size:
1802 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 851 1288 1313 1378 2156 2346 3138 851
Size: 437 25 65 778 190 792 2077
Fragments arranged by size:
2077 792 778 437 190 65 25
BsmI GAATG_Cn'
Cuts at: 996 996
Size: 4364
Bsp1286I G_dGCh'C
Cuts at: 320 624 718 851 1288 1313 1378 1989 2082
Size: 304 94 133 437 25 65 611 93
Cuts at: 2082 2156 2346 2408 3138 320
Size: 74 190 62 730 1546
Fragments arranged by size:
1546 730 611 437 304 190 133 94
93 74 65 62 25
BspEI T'CCGG_A
Cuts at: 3584 3584
Size: 4364
BspHI T'CATG_A
Cuts at: 3540 3540
Size: 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1038 1414 1628 1930 2311 1038
Size: 376 214 302 381 3091
Fragments arranged by size:
3091 381 376 302 214
BsrBI CCG'CTC
Cuts at: 50 2656 2710 4194 4359 50
Size: 2606 54 1484 165 55
Fragments arranged by size:
2606 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2276 2276
Size: 4364
BsrFI r'CCGG_y
Cuts at: 302 2362 2543 2752 302
Size: 2060 181 209 1914
Fragments arranged by size:
2060 1914 209 181
BssSI C'ACGA_G
Cuts at: 2635 2993 2635
Size: 358 4006
BstAPI GCAn_nnn'nTGC
Cuts at: 1741 1741
Size: 4364
BstBI TT'CG_AA
Cuts at: 2725 2725
Size: 4364
BstYI r'GATC_y
Cuts at: 1 767 1389 1856 1879 2214 2460 3472 3558
Size: 766 622 467 23 335 246 1012 86
Cuts at: 3558 3570 3916 1
Size: 12 346 449
Fragments arranged by size:
1012 766 622 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 632 632
Size: 4364
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2314 2495 2658 2314
Size: 181 163 4020
Fragments arranged by size:
4020 181 163
BtsI GCAGTG_nn'
Cuts at: 608 968 1232 1607 608
Size: 360 264 375 3365
Fragments arranged by size:
3365 375 360 264
DraI TTT'AAA
Cuts at: 1363 1363
Size: 4364
DrdI GACnn_nn'nnGTC
Cuts at: 2070 2928 2070
Size: 858 3506
EaeI y'GGCC_r
Cuts at: 734 1002 1949 2123 2514 2541 3886 734
Size: 268 947 174 391 27 1345 1212
Fragments arranged by size:
1345 1212 947 391 268 174 27
EagI C'GGCC_G
Cuts at: 1949 1949
Size: 4364
EarI CTCTTCn'nnn_
Cuts at: 881 2387 2597 881
Size: 1506 210 2648
Fragments arranged by size:
2648 1506 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 761 2572 2882 3028 4174 761
Size: 1811 310 146 1146 951
Fragments arranged by size:
1811 1146 951 310 146
EcoICRI GAG'CTC
Cuts at: 1311 1376 1311
Size: 65 4299
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 2189 2621 3368 3636 484
Size: 1705 432 747 268 1212
Fragments arranged by size:
1705 1212 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1503 1503
Size: 4364
EcoRI G'AATT_C
Cuts at: 1368 1368
Size: 4364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 354 966 2383 354
Size: 612 1417 2335
Fragments arranged by size:
2335 1417 612
FseI GG_CCGG'CC
Cuts at: 306 306
Size: 4364
FspI TGC'GCA
Cuts at: 2145 2145
Size: 4364
HaeII r_GCGC'y
Cuts at: 1798 2046 3068 4047 1798
Size: 248 1022 979 2115
Fragments arranged by size:
2115 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 319 351 901 933 2206 2236 2238 2268 319
Size: 32 550 32 1273 30 2 30 2415
Fragments arranged by size:
2415 1273 550 32 32 30 30 2
HincII GTy'rAC
Cuts at: 177 960 1403 177
Size: 783 443 3138
Fragments arranged by size:
3138 783 443
HindIII A'AGCT_T
Cuts at: 551 1145 551
Size: 594 3770
KasI G'GCGC_C
Cuts at: 1794 2042 1794
Size: 248 4116
KpnI G_GTAC'C
Cuts at: 1384 1384
Size: 4364
MluI A'CGCG_T
Cuts at: 3797 3797
Size: 4364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3035 3219 3035
Size: 184 4180
MscI TGG'CCA
Cuts at: 736 1004 2125 736
Size: 268 1121 2975
Fragments arranged by size:
2975 1121 268
MslI CAynn'nnrTG
Cuts at: 2353 2480 2353
Size: 127 4237
MspA1I CmG'CkG
Cuts at: 442 1475 1789 2149 3162 3407 442
Size: 1033 314 360 1013 245 1399
Fragments arranged by size:
1399 1033 1013 360 314 245
NaeI GCC'GGC
Cuts at: 304 2545 304
Size: 2241 2123
NarI GG'CG_CC
Cuts at: 1795 2043 1795
Size: 248 4116
NcoI C'CATG_G
Cuts at: 632 632
Size: 4364
NgoMIV G'CCGG_C
Cuts at: 302 2543 302
Size: 2241 2123
NspI r_CATG'y
Cuts at: 1417 1917 2824 1417
Size: 500 907 2957
Fragments arranged by size:
2957 907 500
PciI A'CATG_T
Cuts at: 2820 2820
Size: 4364
PflMI CCAn_nnn'nTGG
Cuts at: 445 445
Size: 4364
PfoI T'CCnGG_A
Cuts at: 1313 1313
Size: 4364
PmeI GTTT'AAAC
Cuts at: 1363 1363
Size: 4364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1152 1184 3503 3535 1152
Size: 32 2319 32 1981
Fragments arranged by size:
2319 1981 32 32
PshAI GACnn'nnGTC
Cuts at: 1055 1055
Size: 4364
PstI C_TGCA'G
Cuts at: 1411 2096 1411
Size: 685 3679
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4364
PvuII CAG'CTG
Cuts at: 442 1789 2149 442
Size: 1347 360 2657
Fragments arranged by size:
2657 1347 360
RsrII CG'GwC_CG
Cuts at: 2559 2559
Size: 4364
SacI G_AGCT'C
Cuts at: 1313 1378 1313
Size: 65 4299
SalI G'TCGA_C
Cuts at: 175 1401 175
Size: 1226 3138
SapI GCTCTTCn'nnn_
Cuts at: 2387 2597 2387
Size: 210 4154
SbfI CC_TGCA'GG
Cuts at: 1411 1411
Size: 4364
ScaI AGT'ACT
Cuts at: 334 3881 334
Size: 3547 817
SfcI C'TryA_G
Cuts at: 33 353 1407 2092 3085 3276 33
Size: 320 1054 685 993 191 1121
Fragments arranged by size:
1121 1054 993 685 320 191
SfoI GGC'GCC
Cuts at: 1796 2044 1796
Size: 248 4116
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
SmaI CCC'GGG
Cuts at: 1156 1386 1156
Size: 230 4134
SmlI C'TyrA_G
Cuts at: 841 1519 2434 2926 3188 841
Size: 678 915 492 262 2017
Fragments arranged by size:
2017 915 678 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4364
SphI G_CATG'C
Cuts at: 1417 1917 1417
Size: 500 3864
SspI AAT'ATT
Cuts at: 1356 1356
Size: 4364
StuI AGG'CCT
Cuts at: 706 706
Size: 4364
StyI C'CwwG_G
Cuts at: 297 632 707 1498 1852 297
Size: 335 75 791 354 2809
Fragments arranged by size:
2809 791 354 335 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1947 2080 2539 2748 1947
Size: 133 459 209 3563
Fragments arranged by size:
3563 459 209 133
TatI w'GTAC_w
Cuts at: 332 3879 332
Size: 3547 817
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3881 3913 3881
Size: 32 4332
Tth111I GACn'n_nGTC
Cuts at: 796 964 2161 796
Size: 168 1197 2999
Fragments arranged by size:
2999 1197 168
XbaI T'CTAG_A
Cuts at: 1395 1395
Size: 4364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1170 1285 1859 1170
Size: 115 574 3675
Fragments arranged by size:
3675 574 115
XmaI C'CCGG_G
Cuts at: 1154 1384 1154
Size: 230 4134
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI ApaI AscI BaeI
BbsI BcgI BfrBI BmgBI BmtI BseRI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI