Restriction map for
pF1KB5302
(Circular) (Six-base) MAPSORT of: pF1KB5302.seq Check: 8967 from: 1 to: 4040
pF1KB5302 4040 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 603 1078 603
Size: 475 3565
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1865 2297 2650 3312 1865
Size: 432 353 662 2593
Fragments arranged by size:
2593 662 432 353
AflII C'TTAA_G
Cuts at: 415 415
Size: 4040
AflIII A'CryG_T
Cuts at: 1002 1392 3192 3473 1002
Size: 390 1800 281 1569
Fragments arranged by size:
1800 1569 390 281
AleI CACnn'nnGTG
Cuts at: 814 814
Size: 4040
AlwNI CAG_nnn'CTG
Cuts at: 265 1345 2783 265
Size: 1080 1438 1522
Fragments arranged by size:
1522 1438 1080
ApaLI G'TGCA_C
Cuts at: 2878 2878
Size: 4040
ApoI r'AATT_y
Cuts at: 1044 3393 3649 1044
Size: 2349 256 1435
Fragments arranged by size:
2349 1435 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 746 1060 746
Size: 314 3726
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 857 1056 1470 1718 1753 857
Size: 199 414 248 35 3144
Fragments arranged by size:
3144 414 248 199 35
BanII G_rGCy'C
Cuts at: 828 1054 2084 828
Size: 226 1030 2784
Fragments arranged by size:
2784 1030 226
BbeI G_GCGC'C
Cuts at: 1474 1722 1474
Size: 248 3792
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 232 266 232
Size: 34 4006
BciVI GTATCCnnnnn_n'
Cuts at: 1938 2994 4040 1938
Size: 1056 1046 1938
Fragments arranged by size:
1938 1056 1046
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BglII A'GATC_T
Cuts at: 549 593 1555 3592 549
Size: 44 962 2037 997
Fragments arranged by size:
2037 997 962 44
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 1665 1758 2882 1665
Size: 93 1124 2823
Fragments arranged by size:
2823 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1252 1333 1668 3812 1252
Size: 81 335 2144 1480
Fragments arranged by size:
2144 1480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 2095 2809 3107 1216
Size: 879 714 298 2149
Fragments arranged by size:
2149 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 619 3744 619
Size: 3125 915
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 1471 1719 2421 3917 1471
Size: 248 702 1496 1594
Fragments arranged by size:
1594 1496 702 248
BsaWI w'CCGG_w
Cuts at: 866 1229 1750 2428 2839 2986 3260 866
Size: 363 521 678 411 147 274 1646
Fragments arranged by size:
1646 678 521 411 363 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 350 380 350
Size: 30 4010
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 734 764 734
Size: 30 4010
BseYI C'CCAG_C
Cuts at: 978 1465 2223 2888 3296 978
Size: 487 758 665 408 1722
Fragments arranged by size:
1722 758 665 487 408
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2858 104
Size: 1478 46 1230 1286
Fragments arranged by size:
1478 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 654 828 894 1054 1832 2022 2882 654
Size: 174 66 160 778 190 860 1812
Fragments arranged by size:
1812 860 778 190 174 160 66
Bsp1286I G_dGCh'C
Cuts at: 654 828 894 1054 1665 1758 1832 2022 2084
Size: 174 66 160 611 93 74 190 62
Cuts at: 2084 2882 654
Size: 798 1812
Fragments arranged by size:
1812 798 611 190 174 160 93 74
66 62
BspHI T'CATG_A
Cuts at: 294 459 2472 294
Size: 165 2013 1862
Fragments arranged by size:
2013 1862 165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1090 1304 1606 1987 1090
Size: 214 302 381 3143
Fragments arranged by size:
3143 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1952 1952
Size: 4040
BsrFI r'CCGG_y
Cuts at: 2038 2219 2038
Size: 181 3859
BssSI C'ACGA_G
Cuts at: 2311 3019 2311
Size: 708 3332
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 1417
Size: 4040
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstEII G'GTnAC_C
Cuts at: 916 916
Size: 4040
BstXI CCAn_nnnn'nTGG
Cuts at: 135 135
Size: 4040
BstYI r'GATC_y
Cuts at: 1 549 593 711 1065 1532 1555 1890 2136
Size: 548 44 118 354 467 23 335 246
Cuts at: 2136 2442 2454 2540 3592 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 548 467 449 354 335 306 246
118 86 44 23 12
BtgI C'CryG_G
Cuts at: 107 723 107
Size: 616 3424
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 400 572 1283 400
Size: 172 711 3157
Fragments arranged by size:
3157 711 172
DraI TTT'AAA
Cuts at: 1039 1039
Size: 4040
DrdI GACnn_nn'nnGTC
Cuts at: 1746 3090 1746
Size: 1344 2696
EaeI y'GGCC_r
Cuts at: 1625 1799 2190 2217 3562 1625
Size: 174 391 27 1345 2103
Fragments arranged by size:
2103 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2248 2990 3136 3850 2248
Size: 742 146 714 2438
Fragments arranged by size:
2438 742 714 146
EcoICRI GAG'CTC
Cuts at: 826 1052 826
Size: 226 3814
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1865 2297 2650 3312 1865
Size: 432 353 662 2593
Fragments arranged by size:
2593 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 989 989
Size: 4040
EcoO109I rG'GnC_Cy
Cuts at: 1179 1179
Size: 4040
EcoRI G'AATT_C
Cuts at: 1044 1044
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 4040
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 1474 1722 2952 3723 1474
Size: 248 1230 771 1791
Fragments arranged by size:
1791 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 585 617 777 809 1882 1912 1914 1944 585
Size: 32 160 32 1073 30 2 30 2681
Fragments arranged by size:
2681 1073 160 32 32 30 30 2
HincII GTy'rAC
Cuts at: 369 1079 369
Size: 710 3330
KasI G'GCGC_C
Cuts at: 1470 1718 1470
Size: 248 3792
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 260 2799 2983 260
Size: 2539 184 1317
Fragments arranged by size:
2539 1317 184
MscI TGG'CCA
Cuts at: 1801 1801
Size: 4040
MslI CAynn'nnrTG
Cuts at: 800 814 2029 2156 800
Size: 14 1215 127 2684
Fragments arranged by size:
2684 1215 127 14
MspA1I CmG'CkG
Cuts at: 156 174 1151 1465 1825 2609 2854 156
Size: 18 977 314 360 784 245 1342
Fragments arranged by size:
1342 977 784 360 314 245 18
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 1471 1719 1471
Size: 248 3792
NcoI C'CATG_G
Cuts at: 107 107
Size: 4040
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NspI r_CATG'y
Cuts at: 1006 1093 1593 3196 1006
Size: 87 500 1603 1850
Fragments arranged by size:
1850 1603 500 87
PciI A'CATG_T
Cuts at: 1002 3192 1002
Size: 2190 1850
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 106 138 2486 2518 106
Size: 32 2348 32 1628
Fragments arranged by size:
2348 1628 32 32
PspXI vC'TCGA_Gb
Cuts at: 746 746
Size: 4040
PstI C_TGCA'G
Cuts at: 1087 1772 1087
Size: 685 3355
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 156 174 1465 1825 156
Size: 18 1291 360 2371
Fragments arranged by size:
2371 1291 360 18
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 828 1054 828
Size: 226 3814
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SfcI C'TryA_G
Cuts at: 33 626 1083 1768 2736 2927 33
Size: 593 457 685 968 191 1146
Fragments arranged by size:
1146 968 685 593 457 191
SfoI GGC'GCC
Cuts at: 1472 1720 1472
Size: 248 3792
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 415 746 1195 2110 2824 3086 415
Size: 331 449 915 714 262 1369
Fragments arranged by size:
1369 915 714 449 331 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
SspI AAT'ATT
Cuts at: 541 541
Size: 4040
StyI C'CwwG_G
Cuts at: 107 128 578 1174 1528 107
Size: 21 450 596 354 2619
Fragments arranged by size:
2619 596 450 354 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1623 1756 2215 2424 1623
Size: 133 459 209 3239
Fragments arranged by size:
3239 459 209 133
TatI w'GTAC_w
Cuts at: 3555 3555
Size: 4040
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4008
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 820 1071 820
Size: 251 3789
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1535 1535
Size: 4040
XhoI C'TCGA_G
Cuts at: 746 746
Size: 4040
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
XmnI GAAnn'nnTTC
Cuts at: 121 121
Size: 4040
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsgI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI ZraI