Restriction map for
pF1KB5306
(Circular) (Six-base) MAPSORT of: pF1KB5306.seq Check: 1072 from: 1 to: 5051
pF1KB5306 5051 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 769 1560 2089 769
Size: 791 529 3731
Fragments arranged by size:
3731 791 529
Acc65I G'GTAC_C
Cuts at: 2067 2067
Size: 5051
AclI AA'CG_TT
Cuts at: 4891 4891
Size: 5051
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1709 2876 3308 3661 4323 1709
Size: 1167 432 353 662 2437
Fragments arranged by size:
2437 1167 662 432 353
AflIII A'CryG_T
Cuts at: 2403 4203 4484 2403
Size: 1800 281 2970
Fragments arranged by size:
2970 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1713 1713
Size: 5051
AleI CACnn'nnGTG
Cuts at: 791 791
Size: 5051
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1646 1678 1646
Size: 32 5019
AlwNI CAG_nnn'CTG
Cuts at: 2356 3794 2356
Size: 1438 3613
ApaI G_GGCC'C
Cuts at: 2027 2027
Size: 5051
ApaLI G'TGCA_C
Cuts at: 3889 3889
Size: 5051
ApoI r'AATT_y
Cuts at: 1725 1879 2055 4404 4660 1725
Size: 154 176 2349 256 2116
Fragments arranged by size:
2349 2116 256 176 154
AseI AT'TA_AT
Cuts at: 20 626 20
Size: 606 4445
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5051
AvaI C'yCGr_G
Cuts at: 1511 2071 1511
Size: 560 4491
BamHI G'GATC_C
Cuts at: 2076 2076
Size: 5051
BanI G'GyrC_C
Cuts at: 142 1042 2067 2481 2729 2764 142
Size: 900 1025 414 248 35 2429
Fragments arranged by size:
2429 1025 900 414 248 35
BanII G_rGCy'C
Cuts at: 1998 2027 2065 3095 1998
Size: 29 38 1030 3954
Fragments arranged by size:
3954 1030 38 29
BbeI G_GCGC'C
Cuts at: 2485 2733 2485
Size: 248 4803
BbsI GAAGACnn'nnnn_
Cuts at: 458 523 1652 458
Size: 65 1129 3857
Fragments arranged by size:
3857 1129 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 175 209 175
Size: 34 5017
BciVI GTATCCnnnnn_n'
Cuts at: 1144 2949 4005 5051 1144
Size: 1805 1056 1046 1144
Fragments arranged by size:
1805 1144 1056 1046
BclI T'GATC_A
Cuts at: 201 2571 201
Size: 2370 2681
BglII A'GATC_T
Cuts at: 2566 4603 2566
Size: 2037 3014
BlpI GC'TnA_GC
Cuts at: 917 2163 917
Size: 1246 3805
Bme1580I G_kGCm'C
Cuts at: 1008 2027 2676 2769 3893 1008
Size: 1019 649 93 1124 2166
Fragments arranged by size:
2166 1124 1019 649 93
BmrI ACTGGGnnnn_n'
Cuts at: 1048 2263 2344 2679 4823 1048
Size: 1215 81 335 2144 1276
Fragments arranged by size:
2144 1276 1215 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3418 3418
Size: 5051
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 797 1271 1924 797
Size: 474 653 3924
Fragments arranged by size:
3924 653 474
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1753 2227 3106 3820 4118 1753
Size: 474 879 714 298 2686
Fragments arranged by size:
2686 879 714 474 298
BsaI GGTCTCn'nnnn_
Cuts at: 430 610 4755 430
Size: 180 4145 726
Fragments arranged by size:
4145 726 180
BsaAI yAC'GTr
Cuts at: 2404 2404
Size: 5051
BsaBI GATnn'nnATC
Cuts at: 6 1612 1856 2565 4602 6
Size: 1606 244 709 2037 455
Fragments arranged by size:
2037 1606 709 455 244
BsaHI Gr'CG_yC
Cuts at: 891 2482 2730 3432 4928 891
Size: 1591 248 702 1496 1014
Fragments arranged by size:
1591 1496 1014 702 248
BsaWI w'CCGG_w
Cuts at: 2240 2761 3439 3850 3997 4271 2240
Size: 521 678 411 147 274 3020
Fragments arranged by size:
3020 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 472 1289 1988 472
Size: 817 699 3535
Fragments arranged by size:
3535 817 699
BseYI C'CCAG_C
Cuts at: 2476 3234 3899 4307 2476
Size: 758 665 408 3220
Fragments arranged by size:
3220 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1244 1490 1967 1244
Size: 246 477 4328
Fragments arranged by size:
4328 477 246
BsiEI CG_ry'CG
Cuts at: 104 2593 2639 3869 104
Size: 2489 46 1230 1286
Fragments arranged by size:
2489 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 695 1998 2065 2843 3033 3893 695
Size: 1303 67 778 190 860 1853
Fragments arranged by size:
1853 1303 860 778 190 67
BsmI GAATG_Cn'
Cuts at: 1029 1973 1029
Size: 944 4107
Bsp1286I G_dGCh'C
Cuts at: 695 1008 1998 2027 2065 2676 2769 2843 3033
Size: 313 990 29 38 611 93 74 190
Cuts at: 3033 3095 3893 695
Size: 62 798 1853
Fragments arranged by size:
1853 990 798 611 313 190 93 74
62 38 29
BspHI T'CATG_A
Cuts at: 1101 1334 3483 1101
Size: 233 2149 2669
Fragments arranged by size:
2669 2149 233
BspMI ACCTGCnnnn'nnnn_
Cuts at: 129 1478 2101 2315 2617 2998 129
Size: 1349 623 214 302 381 2182
Fragments arranged by size:
2182 1349 623 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3343 3397 4881 5046 50
Size: 3293 54 1484 165 55
Fragments arranged by size:
3293 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 293 2963 293
Size: 2670 2381
BsrFI r'CCGG_y
Cuts at: 3049 3230 3049
Size: 181 4870
BssSI C'ACGA_G
Cuts at: 3322 4030 3322
Size: 708 4343
BstAPI GCAn_nnn'nTGC
Cuts at: 2428 2428
Size: 5051
BstBI TT'CG_AA
Cuts at: 3412 3412
Size: 5051
BstXI CCAn_nnnn'nTGG
Cuts at: 534 793 534
Size: 259 4792
BstYI r'GATC_y
Cuts at: 1 2076 2543 2566 2901 3147 3453 3465 3551
Size: 2075 467 23 335 246 306 12 86
Cuts at: 3551 4603 1
Size: 1052 449
Fragments arranged by size:
2075 1052 467 449 335 306 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1561 1561
Size: 5051
Bsu36I CC'TnA_GG
Cuts at: 2019 2019
Size: 5051
BtgI C'CryG_G
Cuts at: 1038 1038
Size: 5051
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3001 3182 3345 3001
Size: 181 163 4707
Fragments arranged by size:
4707 181 163
BtsI GCAGTG_nn'
Cuts at: 2294 2294
Size: 5051
ClaI AT'CG_AT
Cuts at: 959 959
Size: 5051
DraI TTT'AAA
Cuts at: 2050 2050
Size: 5051
DrdI GACnn_nn'nnGTC
Cuts at: 2757 4101 2757
Size: 1344 3707
EaeI y'GGCC_r
Cuts at: 2636 2810 3201 3228 4573 2636
Size: 174 391 27 1345 3114
Fragments arranged by size:
3114 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2636 2636
Size: 5051
EarI CTCTTCn'nnn_
Cuts at: 936 2030 3074 3284 936
Size: 1094 1044 210 2703
Fragments arranged by size:
2703 1094 1044 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3259 4001 4147 4861 3259
Size: 742 146 714 3449
Fragments arranged by size:
3449 742 714 146
EcoICRI GAG'CTC
Cuts at: 1996 2063 1996
Size: 67 4984
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 797 1271 1709 1924 2876 3308 3661 4323 797
Size: 474 438 215 952 432 353 662 1525
Fragments arranged by size:
1525 952 662 474 438 432 353 215
EcoO109I rG'GnC_Cy
Cuts at: 118 2023 2190 118
Size: 1905 167 2979
Fragments arranged by size:
2979 1905 167
EcoRI G'AATT_C
Cuts at: 1725 2055 1725
Size: 330 4721
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 526 3070 526
Size: 2544 2507
FspI TGC'GCA
Cuts at: 2832 2832
Size: 5051
HaeII r_GCGC'y
Cuts at: 2485 2733 3963 4734 2485
Size: 248 1230 771 2802
Fragments arranged by size:
2802 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 448 480 751 783 948 980 1699 1731 2893
Size: 32 271 32 165 32 719 32 1162
Cuts at: 2893 2923 2925 2955 448
Size: 30 2 30 2544
Fragments arranged by size:
2544 1162 719 271 165 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 822 2090 822
Size: 1268 3783
HindIII A'AGCT_T
Cuts at: 444 1150 444
Size: 706 4345
KasI G'GCGC_C
Cuts at: 2481 2729 2481
Size: 248 4803
KpnI G_GTAC'C
Cuts at: 2071 2071
Size: 5051
MluI A'CGCG_T
Cuts at: 4484 4484
Size: 5051
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 654 3810 3994 654
Size: 3156 184 1711
Fragments arranged by size:
3156 1711 184
MscI TGG'CCA
Cuts at: 2812 2812
Size: 5051
MslI CAynn'nnrTG
Cuts at: 791 3040 3167 791
Size: 2249 127 2675
Fragments arranged by size:
2675 2249 127
MspA1I CmG'CkG
Cuts at: 775 1228 2162 2476 2836 3620 3865 775
Size: 453 934 314 360 784 245 1961
Fragments arranged by size:
1961 934 784 453 360 314 245
NaeI GCC'GGC
Cuts at: 3232 3232
Size: 5051
NarI GG'CG_CC
Cuts at: 2482 2730 2482
Size: 248 4803
NgoMIV G'CCGG_C
Cuts at: 3230 3230
Size: 5051
NspI r_CATG'y
Cuts at: 1959 2104 2604 4207 1959
Size: 145 500 1603 2803
Fragments arranged by size:
2803 1603 500 145
PciI A'CATG_T
Cuts at: 4203 4203
Size: 5051
PfoI T'CCnGG_A
Cuts at: 1664 1664
Size: 5051
PmeI GTTT'AAAC
Cuts at: 2050 2050
Size: 5051
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 3497
Size: 32 5019
PpuMI rG'GwC_Cy
Cuts at: 118 118
Size: 5051
PspOMI G'GGCC_C
Cuts at: 2023 2023
Size: 5051
PstI C_TGCA'G
Cuts at: 126 2098 2783 126
Size: 1972 685 2394
Fragments arranged by size:
2394 1972 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5051
PvuII CAG'CTG
Cuts at: 775 1228 2476 2836 775
Size: 453 1248 360 2990
Fragments arranged by size:
2990 1248 453 360
RsrII CG'GwC_CG
Cuts at: 3246 3246
Size: 5051
SacI G_AGCT'C
Cuts at: 1998 2065 1998
Size: 67 4984
SalI G'TCGA_C
Cuts at: 2088 2088
Size: 5051
SapI GCTCTTCn'nnn_
Cuts at: 3074 3284 3074
Size: 210 4841
SbfI CC_TGCA'GG
Cuts at: 2098 2098
Size: 5051
ScaI AGT'ACT
Cuts at: 4568 4568
Size: 5051
SfcI C'TryA_G
Cuts at: 33 122 770 2094 2779 3747 3938 33
Size: 89 648 1324 685 968 191 1146
Fragments arranged by size:
1324 1146 968 685 648 191 89
SfoI GGC'GCC
Cuts at: 2483 2731 2483
Size: 248 4803
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5051
SmaI CCC'GGG
Cuts at: 2073 2073
Size: 5051
SmlI C'TyrA_G
Cuts at: 1732 2206 3121 3835 4097 1732
Size: 474 915 714 262 2686
Fragments arranged by size:
2686 915 714 474 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5051
SphI G_CATG'C
Cuts at: 1959 2104 2604 1959
Size: 145 500 4406
Fragments arranged by size:
4406 500 145
StyI C'CwwG_G
Cuts at: 113 410 510 1308 2185 2539 113
Size: 297 100 798 877 354 2625
Fragments arranged by size:
2625 877 798 354 297 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 151 781 799 955 2634 2767 3226 3435 151
Size: 630 18 156 1679 133 459 209 1767
Fragments arranged by size:
1767 1679 630 459 209 156 133 18
TatI w'GTAC_w
Cuts at: 612 1679 4566 612
Size: 1067 2887 1097
Fragments arranged by size:
2887 1097 1067
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4568 4600 4568
Size: 32 5019
Tth111I GACn'n_nGTC
Cuts at: 2848 2848
Size: 5051
XbaI T'CTAG_A
Cuts at: 2082 2082
Size: 5051
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2546 2546
Size: 5051
XmaI C'CCGG_G
Cuts at: 2071 2071
Size: 5051
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AlfI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmBI BspEI BsrGI BssHII BstEII CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI