Restriction map for
pF1KB5307
(Circular) (Six-base) MAPSORT of: pF1KB5307.seq Check: 1196 from: 1 to: 4349
pF1KB5307 4349 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AatII G_ACGT'C
Cuts at: 205 1295 205
Size: 1090 3259
AccI GT'mk_AC
Cuts at: 1295 1387 1295
Size: 92 4257
Acc65I G'GTAC_C
Cuts at: 383 1365 383
Size: 982 3367
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2606 3353 3621 2174
Size: 432 747 268 2902
Fragments arranged by size:
2902 747 432 268
AfeI AGC'GCT
Cuts at: 244 244
Size: 4349
AflIII A'CryG_T
Cuts at: 1701 2805 3782 1701
Size: 1104 977 2268
Fragments arranged by size:
2268 1104 977
AgeI A'CCGG_T
Cuts at: 2737 2737
Size: 4349
AlwNI CAG_nnn'CTG
Cuts at: 513 582 1272 1654 3221 513
Size: 69 690 382 1567 1641
Fragments arranged by size:
1641 1567 690 382 69
ApaI G_GGCC'C
Cuts at: 195 195
Size: 4349
ApaLI G'TGCA_C
Cuts at: 3119 3119
Size: 4349
ApoI r'AATT_y
Cuts at: 1353 3702 3958 1353
Size: 2349 256 1744
Fragments arranged by size:
2349 1744 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 139 236 1369 139
Size: 97 1133 3119
Fragments arranged by size:
3119 1133 97
BamHI G'GATC_C
Cuts at: 1374 1374
Size: 4349
BanI G'GyrC_C
Cuts at: 383 1177 1365 1779 2027 2062 383
Size: 794 188 414 248 35 2670
Fragments arranged by size:
2670 794 414 248 188 35
BanII G_rGCy'C
Cuts at: 195 1363 2393 195
Size: 1168 1030 2151
Fragments arranged by size:
2151 1168 1030
BbeI G_GCGC'C
Cuts at: 1181 1783 2031 1181
Size: 602 248 3499
Fragments arranged by size:
3499 602 248
BbvCI CC'TCA_GC
Cuts at: 127 1115 127
Size: 988 3361
BciVI GTATCCnnnnn_n'
Cuts at: 2247 3008 4349 2247
Size: 761 1341 2247
Fragments arranged by size:
2247 1341 761
BclI T'GATC_A
Cuts at: 1034 1869 1034
Size: 835 3514
BfrBI ATG'CAT
Cuts at: 1170 1170
Size: 4349
BglII A'GATC_T
Cuts at: 1077 1864 3901 1077
Size: 787 2037 1525
Fragments arranged by size:
2037 1525 787
BlpI GC'TnA_GC
Cuts at: 1461 1461
Size: 4349
Bme1580I G_kGCm'C
Cuts at: 195 1974 2067 3123 195
Size: 1779 93 1056 1421
Fragments arranged by size:
1779 1421 1056 93
BmgBI CAC'GTC
Cuts at: 1223 1223
Size: 4349
BmrI ACTGGGnnnn_n'
Cuts at: 549 1176 1561 1642 1977 4121 549
Size: 627 385 81 335 2144 777
Fragments arranged by size:
2144 777 627 385 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 321 431 2716 321
Size: 110 2285 1954
Fragments arranged by size:
2285 1954 110
Bpu10I CC'TnA_GC
Cuts at: 127 1115 127
Size: 988 3361
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1525 2404 2896 3194 922
Size: 603 879 492 298 2077
Fragments arranged by size:
2077 879 603 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1259 4053 1259
Size: 2794 1555
BsaAI yAC'GTr
Cuts at: 225 480 628 1702 225
Size: 255 148 1074 2872
Fragments arranged by size:
2872 1074 255 148
BsaBI GATnn'nnATC
Cuts at: 6 1028 1863 3900 6
Size: 1022 835 2037 455
Fragments arranged by size:
2037 1022 835 455
BsaHI Gr'CG_yC
Cuts at: 118 202 825 1178 1292 1780 2028 2730 4226
Size: 84 623 353 114 488 248 702 1496
Cuts at: 4226 118
Size: 241
Fragments arranged by size:
1496 702 623 488 353 248 241 114
84
BsaWI w'CCGG_w
Cuts at: 1337 1538 2059 2737 3011 3158 3569 1337
Size: 201 521 678 274 147 411 2117
Fragments arranged by size:
2117 678 521 411 274 201 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 170 200 170
Size: 30 4319
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 144 144
Size: 4349
BseYI C'CCAG_C
Cuts at: 1774 2532 3109 3605 1774
Size: 758 577 496 2518
Fragments arranged by size:
2518 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 460
Size: 4349
BsiEI CG_ry'CG
Cuts at: 104 1891 1937 3145 104
Size: 1787 46 1208 1308
Fragments arranged by size:
1787 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 381 839 1363 2141 2331 3123 381
Size: 458 524 778 190 792 1607
Fragments arranged by size:
1607 792 778 524 458 190
BsmI GAATG_Cn'
Cuts at: 948 948
Size: 4349
BsmBI CGTCTCn'nnnn_
Cuts at: 784 784
Size: 4349
Bsp1286I G_dGCh'C
Cuts at: 195 381 839 1363 1974 2067 2141 2331 2393
Size: 186 458 524 611 93 74 190 62
Cuts at: 2393 3123 195
Size: 730 1421
Fragments arranged by size:
1421 730 611 524 458 190 186 93
74 62
BspEI T'CCGG_A
Cuts at: 1337 3569 1337
Size: 2232 2117
BspHI T'CATG_A
Cuts at: 3525 3525
Size: 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 395 1307 1399 1613 1915 2296 395
Size: 912 92 214 302 381 2448
Fragments arranged by size:
2448 912 381 302 214 92
BsrBI CCG'CTC
Cuts at: 50 134 156 197 347 2641 2695 4179 4344
Size: 84 22 41 150 2294 54 1484 165
Cuts at: 4344 50
Size: 55
Fragments arranged by size:
2294 1484 165 150 84 55 54 41
22
BsrDI GCAATG_nn'
Cuts at: 855 2261 855
Size: 1406 2943
BsrFI r'CCGG_y
Cuts at: 2347 2528 2737 2347
Size: 181 209 3959
Fragments arranged by size:
3959 209 181
BssSI C'ACGA_G
Cuts at: 2620 2978 2620
Size: 358 3991
BstAPI GCAn_nnn'nTGC
Cuts at: 1272 1726 1272
Size: 454 3895
BstBI TT'CG_AA
Cuts at: 2710 2710
Size: 4349
BstEII G'GTnAC_C
Cuts at: 603 603
Size: 4349
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1318 114
Size: 1204 3145
BstYI r'GATC_y
Cuts at: 1 1043 1077 1374 1841 1864 2199 2445 3457
Size: 1042 34 297 467 23 335 246 1012
Cuts at: 3457 3543 3555 3901 1
Size: 86 12 346 449
Fragments arranged by size:
1042 1012 467 449 346 335 297 246
86 34 23 12
BtgI C'CryG_G
Cuts at: 1053 1053
Size: 4349
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 996 2299 2480 2643 996
Size: 1303 181 163 2702
Fragments arranged by size:
2702 1303 181 163
BtsI GCAGTG_nn'
Cuts at: 1592 1592
Size: 4349
ClaI AT'CG_AT
Cuts at: 1316 1316
Size: 4349
DraI TTT'AAA
Cuts at: 1348 1348
Size: 4349
DraIII CAC_nnn'GTG
Cuts at: 225 225
Size: 4349
DrdI GACnn_nn'nnGTC
Cuts at: 2055 2913 2055
Size: 858 3491
EaeI y'GGCC_r
Cuts at: 206 278 342 716 873 1155 1320 1934 2108
Size: 72 64 374 157 282 165 614 174
Cuts at: 2108 2499 2526 3871 206
Size: 391 27 1345 684
Fragments arranged by size:
1345 684 614 391 374 282 174 165
157 72 64 27
EagI C'GGCC_G
Cuts at: 1934 1934
Size: 4349
EarI CTCTTCn'nnn_
Cuts at: 2372 2582 2372
Size: 210 4139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 301 559 1297 2557 2867 3013 4159 301
Size: 258 738 1260 310 146 1146 491
Fragments arranged by size:
1260 1146 738 491 310 258 146
EcoICRI GAG'CTC
Cuts at: 1361 1361
Size: 4349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2606 3353 3621 2174
Size: 432 747 268 2902
Fragments arranged by size:
2902 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 172 621 1131 1189 1488 172
Size: 449 510 58 299 3033
Fragments arranged by size:
3033 510 449 299 58
EcoRI G'AATT_C
Cuts at: 1353 1353
Size: 4349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 771 2368 771
Size: 1597 2752
FspI TGC'GCA
Cuts at: 2130 2130
Size: 4349
HaeII r_GCGC'y
Cuts at: 246 736 1181 1783 2031 3053 4032 246
Size: 490 445 602 248 1022 979 563
Fragments arranged by size:
1022 979 602 563 490 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 120 152 164 196 2191 2221 2223 2253 120
Size: 32 12 32 1995 30 2 30 2216
Fragments arranged by size:
2216 1995 32 32 30 30 12 2
HincII GTy'rAC
Cuts at: 1388 1388
Size: 4349
HindIII A'AGCT_T
Cuts at: 1332 1332
Size: 4349
KasI G'GCGC_C
Cuts at: 1177 1779 2027 1177
Size: 602 248 3499
Fragments arranged by size:
3499 602 248
KpnI G_GTAC'C
Cuts at: 387 1369 387
Size: 982 3367
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3020 3204 3020
Size: 184 4165
MscI TGG'CCA
Cuts at: 718 875 1157 2110 718
Size: 157 282 953 2957
Fragments arranged by size:
2957 953 282 157
MslI CAynn'nnrTG
Cuts at: 328 482 596 842 904 1316 2338 2465 328
Size: 154 114 246 62 412 1022 127 2212
Fragments arranged by size:
2212 1022 412 246 154 127 114 62
MspA1I CmG'CkG
Cuts at: 295 437 567 579 1263 1460 1774 2134 3147
Size: 142 130 12 684 197 314 360 1013
Cuts at: 3147 3392 295
Size: 245 1252
Fragments arranged by size:
1252 1013 684 360 314 245 197 142
130 12
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 4349
NarI GG'CG_CC
Cuts at: 1178 1780 2028 1178
Size: 602 248 3499
Fragments arranged by size:
3499 602 248
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 4349
NsiI A_TGCA'T
Cuts at: 1172 1172
Size: 4349
NspI r_CATG'y
Cuts at: 714 1170 1402 1902 2809 714
Size: 456 232 500 907 2254
Fragments arranged by size:
2254 907 500 456 232
PciI A'CATG_T
Cuts at: 2805 2805
Size: 4349
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PmlI CAC'GTG
Cuts at: 225 480 225
Size: 255 4094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 3488
Size: 32 4317
PpuMI rG'GwC_Cy
Cuts at: 172 1131 1189 172
Size: 959 58 3332
Fragments arranged by size:
3332 959 58
PspOMI G'GGCC_C
Cuts at: 191 191
Size: 4349
PstI C_TGCA'G
Cuts at: 392 1262 1268 1277 1396 2081 392
Size: 870 6 9 119 685 2660
Fragments arranged by size:
2660 870 685 119 9 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 567 579 1263 1774 2134 567
Size: 12 684 511 360 2782
Fragments arranged by size:
2782 684 511 360 12
RsrII CG'GwC_CG
Cuts at: 2544 2544
Size: 4349
SacI G_AGCT'C
Cuts at: 1363 1363
Size: 4349
SalI G'TCGA_C
Cuts at: 1386 1386
Size: 4349
SanDI GG'GwC_CC
Cuts at: 172 172
Size: 4349
SapI GCTCTTCn'nnn_
Cuts at: 2372 2582 2372
Size: 210 4139
SbfI CC_TGCA'GG
Cuts at: 1277 1396 1277
Size: 119 4230
ScaI AGT'ACT
Cuts at: 559 3866 559
Size: 3307 1042
SfcI C'TryA_G
Cuts at: 33 388 1258 1264 1273 1392 2077 3070 3261
Size: 355 870 6 9 119 685 993 191
Cuts at: 3261 33
Size: 1121
Fragments arranged by size:
1121 993 870 685 355 191 119 9
6
SfoI GGC'GCC
Cuts at: 1179 1781 2029 1179
Size: 602 248 3499
Fragments arranged by size:
3499 602 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SmaI CCC'GGG
Cuts at: 141 1371 141
Size: 1230 3119
SmlI C'TyrA_G
Cuts at: 937 1504 2419 2911 3173 937
Size: 567 915 492 262 2113
Fragments arranged by size:
2113 915 567 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 1402 1902 1402
Size: 500 3849
StyI C'CwwG_G
Cuts at: 1483 1837 1483
Size: 354 3995
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 798 1932 2065 2524 2733 798
Size: 1134 133 459 209 2414
Fragments arranged by size:
2414 1134 459 209 133
TatI w'GTAC_w
Cuts at: 557 772 3864 557
Size: 215 3092 1042
Fragments arranged by size:
3092 1042 215
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 170 202 3866 3898 170
Size: 32 3664 32 621
Fragments arranged by size:
3664 621 32 32
Tth111I GACn'n_nGTC
Cuts at: 757 1087 2146 757
Size: 330 1059 2960
Fragments arranged by size:
2960 1059 330
XbaI T'CTAG_A
Cuts at: 1380 1380
Size: 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 726 1844 726
Size: 1118 3231
XmaI C'CCGG_G
Cuts at: 139 1369 139
Size: 1230 3119
ZraI GAC'GTC
Cuts at: 203 1293 203
Size: 1090 3259
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BcgI BglI BmtI BpmI BsiWI BsrGI
BssHII BstZ17I Bsu36I CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI