Restriction map for
pF1KB5311
(Circular) (Six-base) MAPSORT of: pF1KB5311.seq Check: 4273 from: 1 to: 4511
pF1KB5311 4511 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1549 1549
Size: 4511
Acc65I G'GTAC_C
Cuts at: 1527 1527
Size: 4511
AclI AA'CG_TT
Cuts at: 4351 4351
Size: 4511
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 679 2336 2768 3121 3783 385
Size: 294 1657 432 353 662 1113
Fragments arranged by size:
1657 1113 662 432 353 294
AflII C'TTAA_G
Cuts at: 1435 1435
Size: 4511
AflIII A'CryG_T
Cuts at: 245 1178 1863 3663 3944 245
Size: 933 685 1800 281 812
Fragments arranged by size:
1800 933 812 685 281
AleI CACnn'nnGTG
Cuts at: 478 478
Size: 4511
AlwNI CAG_nnn'CTG
Cuts at: 211 1260 1350 1816 3254 211
Size: 1049 90 466 1438 1468
Fragments arranged by size:
1468 1438 1049 466 90
ApaLI G'TGCA_C
Cuts at: 1204 3349 1204
Size: 2145 2366
ApoI r'AATT_y
Cuts at: 401 1192 1515 3864 4120 401
Size: 791 323 2349 256 792
Fragments arranged by size:
2349 792 791 323 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4511
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4511
AvaI C'yCGr_G
Cuts at: 825 1531 825
Size: 706 3805
BamHI G'GATC_C
Cuts at: 1536 1536
Size: 4511
BanI G'GyrC_C
Cuts at: 1186 1485 1527 1941 2189 2224 1186
Size: 299 42 414 248 35 3473
Fragments arranged by size:
3473 414 299 248 42 35
BanII G_rGCy'C
Cuts at: 350 1253 1525 2555 350
Size: 903 272 1030 2306
Fragments arranged by size:
2306 1030 903 272
BbeI G_GCGC'C
Cuts at: 1945 2193 1945
Size: 248 4263
BbsI GAAGACnn'nnnn_
Cuts at: 565 961 565
Size: 396 4115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1268 1302 1268
Size: 34 4477
BciVI GTATCCnnnnn_n'
Cuts at: 2409 3465 4511 2409
Size: 1056 1046 2409
Fragments arranged by size:
2409 1056 1046
BclI T'GATC_A
Cuts at: 897 2031 897
Size: 1134 3377
BglII A'GATC_T
Cuts at: 2026 4063 2026
Size: 2037 2474
BlpI GC'TnA_GC
Cuts at: 1015 1623 1015
Size: 608 3903
Bme1580I G_kGCm'C
Cuts at: 1208 1327 2136 2229 3353 1208
Size: 119 809 93 1124 2366
Fragments arranged by size:
2366 1124 809 119 93
BmrI ACTGGGnnnn_n'
Cuts at: 680 1723 1804 2139 4283 680
Size: 1043 81 335 2144 908
Fragments arranged by size:
2144 1043 908 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2878 2878
Size: 4511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 196
Size: 4511
Bpu10I CC'TnA_GC
Cuts at: 770 770
Size: 4511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 100 1687 2566 3280 3578 100
Size: 1587 879 714 298 1033
Fragments arranged by size:
1587 1033 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 266 4215 266
Size: 3949 562
BsaAI yAC'GTr
Cuts at: 444 1864 444
Size: 1420 3091
BsaBI GATnn'nnATC
Cuts at: 6 1234 2025 4062 6
Size: 1228 791 2037 455
Fragments arranged by size:
2037 1228 791 455
BsaHI Gr'CG_yC
Cuts at: 1942 2190 2892 4388 1942
Size: 248 702 1496 2065
Fragments arranged by size:
2065 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1026 1700 2221 2899 3310 3457 3731 1026
Size: 674 521 678 411 147 274 1806
Fragments arranged by size:
1806 678 674 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 726 1132 726
Size: 406 4105
BseYI C'CCAG_C
Cuts at: 1936 2694 3359 3767 1936
Size: 758 665 408 2680
Fragments arranged by size:
2680 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 1488 563
Size: 925 3586
BsiEI CG_ry'CG
Cuts at: 104 1066 2053 2099 3329 104
Size: 962 987 46 1230 1286
Fragments arranged by size:
1286 1230 987 962 46
BsiHKAI G_wGCw'C
Cuts at: 1208 1525 2303 2493 3353 1208
Size: 317 778 190 860 2366
Fragments arranged by size:
2366 860 778 317 190
BsmI GAATG_Cn'
Cuts at: 943 1011 943
Size: 68 4443
Bsp1286I G_dGCh'C
Cuts at: 350 1208 1253 1327 1525 2136 2229 2303 2493
Size: 858 45 74 198 611 93 74 190
Cuts at: 2493 2555 3353 350
Size: 62 798 1508
Fragments arranged by size:
1508 858 798 611 198 190 93 74
74 62 45
BspEI T'CCGG_A
Cuts at: 1026 1026
Size: 4511
BspHI T'CATG_A
Cuts at: 593 2943 593
Size: 2350 2161
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1561 1775 2077 2458 1561
Size: 214 302 381 3614
Fragments arranged by size:
3614 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2803 2857 4341 4506 50
Size: 2753 54 1484 165 55
Fragments arranged by size:
2753 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1304 2423 1304
Size: 1119 3392
BsrFI r'CCGG_y
Cuts at: 2509 2690 2509
Size: 181 4330
BsrGI T'GTAC_A
Cuts at: 248 248
Size: 4511
BssSI C'ACGA_G
Cuts at: 2782 3490 2782
Size: 708 3803
BstAPI GCAn_nnn'nTGC
Cuts at: 186 1350 1888 186
Size: 1164 538 2809
Fragments arranged by size:
2809 1164 538
BstBI TT'CG_AA
Cuts at: 2872 2872
Size: 4511
BstXI CCAn_nnnn'nTGG
Cuts at: 984 984
Size: 4511
BstYI r'GATC_y
Cuts at: 1 455 1211 1536 2003 2026 2361 2607 2913
Size: 454 756 325 467 23 335 246 306
Cuts at: 2913 2925 3011 4063 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 756 467 454 449 335 325 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 350 350
Size: 4511
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 529 2461 2642 2805 529
Size: 1932 181 163 2235
Fragments arranged by size:
2235 1932 181 163
BtsI GCAGTG_nn'
Cuts at: 1754 1754
Size: 4511
DraI TTT'AAA
Cuts at: 1510 1510
Size: 4511
DrdI GACnn_nn'nnGTC
Cuts at: 2217 3561 2217
Size: 1344 3167
EaeI y'GGCC_r
Cuts at: 202 395 875 1063 2096 2270 2661 2688 4033
Size: 193 480 188 1033 174 391 27 1345
Cuts at: 4033 202
Size: 680
Fragments arranged by size:
1345 1033 680 480 391 193 188 174
27
EagI C'GGCC_G
Cuts at: 1063 2096 1063
Size: 1033 3478
EarI CTCTTCn'nnn_
Cuts at: 338 1069 2534 2744 338
Size: 731 1465 210 2105
Fragments arranged by size:
2105 1465 731 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2719 3461 3607 4321 2719
Size: 742 146 714 2909
Fragments arranged by size:
2909 742 714 146
EcoICRI GAG'CTC
Cuts at: 1523 1523
Size: 4511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 385 679 2336 2768 3121 3783 196
Size: 189 294 1657 432 353 662 924
Fragments arranged by size:
1657 924 662 432 353 294 189
EcoNI CCTnn'n_nnAGG
Cuts at: 269 269
Size: 4511
EcoO109I rG'GnC_Cy
Cuts at: 1650 1650
Size: 4511
EcoRI G'AATT_C
Cuts at: 401 1515 401
Size: 1114 3397
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 454 2530 454
Size: 2076 2435
FspI TGC'GCA
Cuts at: 2292 2292
Size: 4511
HaeII r_GCGC'y
Cuts at: 160 1945 2193 3423 4194 160
Size: 1785 248 1230 771 477
Fragments arranged by size:
1785 1230 771 477 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 199 231 819 851 882 914 2353 2383 2385
Size: 32 588 32 31 32 1439 30 2
Cuts at: 2385 2415 199
Size: 30 2295
Fragments arranged by size:
2295 1439 588 32 32 32 31 30
30 2
HincII GTy'rAC
Cuts at: 1550 1550
Size: 4511
KasI G'GCGC_C
Cuts at: 1941 2189 1941
Size: 248 4263
KpnI G_GTAC'C
Cuts at: 1531 1531
Size: 4511
MfeI C'AATT_G
Cuts at: 708 708
Size: 4511
MluI A'CGCG_T
Cuts at: 3944 3944
Size: 4511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 188 884 3270 3454 188
Size: 696 2386 184 1245
Fragments arranged by size:
2386 1245 696 184
MscI TGG'CCA
Cuts at: 397 877 2272 397
Size: 480 1395 2636
Fragments arranged by size:
2636 1395 480
MslI CAynn'nnrTG
Cuts at: 478 2500 2627 478
Size: 2022 127 2362
Fragments arranged by size:
2362 2022 127
MspA1I CmG'CkG
Cuts at: 504 1350 1622 1936 2296 3080 3325 504
Size: 846 272 314 360 784 245 1690
Fragments arranged by size:
1690 846 784 360 314 272 245
NaeI GCC'GGC
Cuts at: 2692 2692
Size: 4511
NarI GG'CG_CC
Cuts at: 1942 2190 1942
Size: 248 4263
NcoI C'CATG_G
Cuts at: 350 350
Size: 4511
NgoMIV G'CCGG_C
Cuts at: 2690 2690
Size: 4511
NspI r_CATG'y
Cuts at: 150 249 1182 1564 2064 3667 150
Size: 99 933 382 500 1603 994
Fragments arranged by size:
1603 994 933 500 382 99
PciI A'CATG_T
Cuts at: 245 1178 3663 245
Size: 933 2485 1093
Fragments arranged by size:
2485 1093 933
PmeI GTTT'AAAC
Cuts at: 1510 1510
Size: 4511
PmlI CAC'GTG
Cuts at: 444 444
Size: 4511
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2957 2989 2957
Size: 32 4479
PstI C_TGCA'G
Cuts at: 191 509 779 835 1346 1355 1558 2243 191
Size: 318 270 56 511 9 203 685 2459
Fragments arranged by size:
2459 685 511 318 270 203 56 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4511
PvuII CAG'CTG
Cuts at: 504 1350 1936 2296 504
Size: 846 586 360 2719
Fragments arranged by size:
2719 846 586 360
RsrII CG'GwC_CG
Cuts at: 2706 2706
Size: 4511
SacI G_AGCT'C
Cuts at: 1525 1525
Size: 4511
SalI G'TCGA_C
Cuts at: 1548 1548
Size: 4511
SapI GCTCTTCn'nnn_
Cuts at: 338 2534 2744 338
Size: 2196 210 2105
Fragments arranged by size:
2196 2105 210
SbfI CC_TGCA'GG
Cuts at: 1558 1558
Size: 4511
ScaI AGT'ACT
Cuts at: 4028 4028
Size: 4511
SfcI C'TryA_G
Cuts at: 33 187 505 583 775 831 1342 1351 1554
Size: 154 318 78 192 56 511 9 203
Cuts at: 1554 2239 3207 3398 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 685 511 318 203 192 191
154 78 56 9
SfoI GGC'GCC
Cuts at: 1943 2191 1943
Size: 248 4263
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4511
SmaI CCC'GGG
Cuts at: 1533 1533
Size: 4511
SmlI C'TyrA_G
Cuts at: 115 1435 1666 2581 3295 3557 115
Size: 1320 231 915 714 262 1069
Fragments arranged by size:
1320 1069 915 714 262 231
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4511
SphI G_CATG'C
Cuts at: 150 1564 2064 150
Size: 1414 500 2597
Fragments arranged by size:
2597 1414 500
StyI C'CwwG_G
Cuts at: 350 740 1069 1170 1645 1999 350
Size: 390 329 101 475 354 2862
Fragments arranged by size:
2862 475 390 354 329 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 253 1473 2094 2227 2686 2895 253
Size: 1220 621 133 459 209 1869
Fragments arranged by size:
1869 1220 621 459 209 133
TatI w'GTAC_w
Cuts at: 248 857 4026 248
Size: 609 3169 733
Fragments arranged by size:
3169 733 609
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 287 319 4028 4060 287
Size: 32 3709 32 738
Fragments arranged by size:
3709 738 32 32
Tth111I GACn'n_nGTC
Cuts at: 2308 2308
Size: 4511
XbaI T'CTAG_A
Cuts at: 1542 1542
Size: 4511
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1149 2006 1149
Size: 857 3654
XmaI C'CCGG_G
Cuts at: 1531 1531
Size: 4511
XmnI GAAnn'nnTTC
Cuts at: 929 929
Size: 4511
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PmlI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI