Restriction map for
pF1KB5317
(Circular) (Six-base) MAPSORT of: pF1KB5317.seq Check: 5032 from: 1 to: 4289
pF1KB5317 4289 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AccI GT'mk_AC
Cuts at: 1327 1327
Size: 4289
Acc65I G'GTAC_C
Cuts at: 1305 1305
Size: 4289
AclI AA'CG_TT
Cuts at: 4129 4129
Size: 4289
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 1035 2114 2546 3293 3561 391
Size: 644 1079 432 747 268 1119
Fragments arranged by size:
1119 1079 747 644 432 268
AfeI AGC'GCT
Cuts at: 1048 1048
Size: 4289
AflIII A'CryG_T
Cuts at: 785 1641 2745 3722 785
Size: 856 1104 977 1352
Fragments arranged by size:
1352 1104 977 856
AgeI A'CCGG_T
Cuts at: 2677 2677
Size: 4289
AlwNI CAG_nnn'CTG
Cuts at: 649 867 1117 1131 1194 1594 3161 649
Size: 218 250 14 63 400 1567 1777
Fragments arranged by size:
1777 1567 400 250 218 63 14
ApaLI G'TGCA_C
Cuts at: 3059 3059
Size: 4289
ApoI r'AATT_y
Cuts at: 249 301 1293 3642 3898 249
Size: 52 992 2349 256 640
Fragments arranged by size:
2349 992 640 256 52
AseI AT'TA_AT
Cuts at: 20 20
Size: 4289
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
AvaI C'yCGr_G
Cuts at: 220 1309 220
Size: 1089 3200
BamHI G'GATC_C
Cuts at: 474 1314 474
Size: 840 3449
BanI G'GyrC_C
Cuts at: 829 1236 1305 1719 1967 2002 829
Size: 407 69 414 248 35 3116
Fragments arranged by size:
3116 414 407 248 69 35
BanII G_rGCy'C
Cuts at: 1303 2333 1303
Size: 1030 3259
BbeI G_GCGC'C
Cuts at: 1723 1971 1723
Size: 248 4041
BbsI GAAGACnn'nnnn_
Cuts at: 470 1187 470
Size: 717 3572
BbvCI CC'TCA_GC
Cuts at: 778 778
Size: 4289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 143 177 143
Size: 34 4255
BciVI GTATCCnnnnn_n'
Cuts at: 2187 2948 4289 2187
Size: 761 1341 2187
Fragments arranged by size:
2187 1341 761
BclI T'GATC_A
Cuts at: 1809 1809
Size: 4289
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4289
BglII A'GATC_T
Cuts at: 1804 3841 1804
Size: 2037 2252
BlpI GC'TnA_GC
Cuts at: 997 1401 997
Size: 404 3885
Bme1580I G_kGCm'C
Cuts at: 1914 2007 3063 1914
Size: 93 1056 3140
Fragments arranged by size:
3140 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 183 1501 1582 1917 4061 183
Size: 1318 81 335 2144 411
Fragments arranged by size:
2144 1318 411 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 246 2656 246
Size: 2410 1879
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 489 907 489
Size: 418 3871
Bpu10I CC'TnA_GC
Cuts at: 778 778
Size: 4289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 1027 1465 2344 2836 3134 265
Size: 762 438 879 492 298 1420
Fragments arranged by size:
1420 879 762 492 438 298
BsaI GGTCTCn'nnnn_
Cuts at: 394 3993 394
Size: 3599 690
BsaAI yAC'GTr
Cuts at: 1642 1642
Size: 4289
BsaBI GATnn'nnATC
Cuts at: 6 1803 3840 6
Size: 1797 2037 455
Fragments arranged by size:
2037 1797 455
BsaHI Gr'CG_yC
Cuts at: 1720 1968 2670 4166 1720
Size: 248 702 1496 1843
Fragments arranged by size:
1843 1496 702 248
BsaWI w'CCGG_w
Cuts at: 568 1478 1999 2677 2951 3098 3509 568
Size: 910 521 678 274 147 411 1348
Fragments arranged by size:
1348 910 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 406 409 412 430 595 628 676 701
Size: 213 3 3 18 165 33 48 25
Cuts at: 701 865 1098 1157 193
Size: 164 233 59 3325
Fragments arranged by size:
3325 233 213 165 164 59 48 33
25 18 3 3
BseYI C'CCAG_C
Cuts at: 1714 2472 3049 3545 1714
Size: 758 577 496 2458
Fragments arranged by size:
2458 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 880
Size: 4289
BsiEI CG_ry'CG
Cuts at: 104 1831 1877 3085 104
Size: 1727 46 1208 1308
Fragments arranged by size:
1727 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1274 1303 2081 2271 3063 1274
Size: 29 778 190 792 2500
Fragments arranged by size:
2500 792 778 190 29
Bsp1286I G_dGCh'C
Cuts at: 1274 1303 1914 2007 2081 2271 2333 3063 1274
Size: 29 611 93 74 190 62 730 2500
Fragments arranged by size:
2500 730 611 190 93 74 62 29
BspEI T'CCGG_A
Cuts at: 3509 3509
Size: 4289
BspHI T'CATG_A
Cuts at: 3465 3465
Size: 4289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1193 1339 1553 1855 2236 1193
Size: 146 214 302 381 3246
Fragments arranged by size:
3246 381 302 214 146
BsrBI CCG'CTC
Cuts at: 50 2581 2635 4119 4284 50
Size: 2531 54 1484 165 55
Fragments arranged by size:
2531 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2201 2201
Size: 4289
BsrFI r'CCGG_y
Cuts at: 2287 2468 2677 2287
Size: 181 209 3899
Fragments arranged by size:
3899 209 181
BssSI C'ACGA_G
Cuts at: 2560 2918 2560
Size: 358 3931
BstAPI GCAn_nnn'nTGC
Cuts at: 729 1666 729
Size: 937 3352
BstBI TT'CG_AA
Cuts at: 2650 2650
Size: 4289
BstXI CCAn_nnnn'nTGG
Cuts at: 1102 1102
Size: 4289
BstYI r'GATC_y
Cuts at: 1 383 474 518 1314 1781 1804 2139 2385
Size: 382 91 44 796 467 23 335 246
Cuts at: 2385 3397 3483 3495 3841 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 796 467 449 382 346 335 246
91 86 44 23 12
Bsu36I CC'TnA_GG
Cuts at: 650 1110 650
Size: 460 3829
BtgI C'CryG_G
Cuts at: 107 281 107
Size: 174 4115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2239 2420 2583 2239
Size: 181 163 3945
Fragments arranged by size:
3945 181 163
BtsI GCAGTG_nn'
Cuts at: 1532 1532
Size: 4289
DraI TTT'AAA
Cuts at: 1265 1288 1265
Size: 23 4266
DrdI GACnn_nn'nnGTC
Cuts at: 1995 2853 1995
Size: 858 3431
EaeI y'GGCC_r
Cuts at: 1874 2048 2439 2466 3811 1874
Size: 174 391 27 1345 2352
Fragments arranged by size:
2352 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1874 1874
Size: 4289
EarI CTCTTCn'nnn_
Cuts at: 131 527 533 547 653 671 2312 2522 131
Size: 396 6 14 106 18 1641 210 1898
Fragments arranged by size:
1898 1641 396 210 106 18 14 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 996 1081 2497 2807 2953 4099 996
Size: 85 1416 310 146 1146 1186
Fragments arranged by size:
1416 1186 1146 310 146 85
EcoICRI GAG'CTC
Cuts at: 1301 1301
Size: 4289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 489 907 1035 2114 2546 3293 3561 391
Size: 98 418 128 1079 432 747 268 1119
Fragments arranged by size:
1119 1079 747 432 418 268 128 98
EcoNI CCTnn'n_nnAGG
Cuts at: 721 721
Size: 4289
EcoO109I rG'GnC_Cy
Cuts at: 114 147 171 225 288 1428 114
Size: 33 24 54 63 1140 2975
Fragments arranged by size:
2975 1140 63 54 33 24
EcoRI G'AATT_C
Cuts at: 1293 1293
Size: 4289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 930 2308 930
Size: 1378 2911
FspI TGC'GCA
Cuts at: 2070 2070
Size: 4289
HaeII r_GCGC'y
Cuts at: 1050 1723 1971 2993 3972 1050
Size: 673 248 1022 979 1367
Fragments arranged by size:
1367 1022 979 673 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2131 2161 2163 2193 2131
Size: 30 2 30 4227
Fragments arranged by size:
4227 30 30 2
HincII GTy'rAC
Cuts at: 1328 1328
Size: 4289
KasI G'GCGC_C
Cuts at: 1719 1967 1719
Size: 248 4041
KpnI G_GTAC'C
Cuts at: 1309 1309
Size: 4289
MluI A'CGCG_T
Cuts at: 3722 3722
Size: 4289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1094 2960 3144 1094
Size: 1866 184 2239
Fragments arranged by size:
2239 1866 184
MscI TGG'CCA
Cuts at: 2050 2050
Size: 4289
MslI CAynn'nnrTG
Cuts at: 2278 2405 2278
Size: 127 4162
MspA1I CmG'CkG
Cuts at: 867 1400 1714 2074 3087 3332 867
Size: 533 314 360 1013 245 1824
Fragments arranged by size:
1824 1013 533 360 314 245
NaeI GCC'GGC
Cuts at: 2470 2470
Size: 4289
NarI GG'CG_CC
Cuts at: 1720 1968 1720
Size: 248 4041
NcoI C'CATG_G
Cuts at: 107 281 107
Size: 174 4115
NgoMIV G'CCGG_C
Cuts at: 2468 2468
Size: 4289
NspI r_CATG'y
Cuts at: 789 1342 1842 2749 789
Size: 553 500 907 2329
Fragments arranged by size:
2329 907 553 500
PciI A'CATG_T
Cuts at: 785 2745 785
Size: 1960 2329
PmeI GTTT'AAAC
Cuts at: 1288 1288
Size: 4289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 526 558 3428 3460 526
Size: 32 2870 32 1355
Fragments arranged by size:
2870 1355 32 32
PpuMI rG'GwC_Cy
Cuts at: 147 171 225 288 147
Size: 24 54 63 4148
Fragments arranged by size:
4148 63 54 24
PstI C_TGCA'G
Cuts at: 725 1007 1082 1190 1336 2021 725
Size: 282 75 108 146 685 2993
Fragments arranged by size:
2993 685 282 146 108 75
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4289
PvuII CAG'CTG
Cuts at: 867 1714 2074 867
Size: 847 360 3082
Fragments arranged by size:
3082 847 360
RsrII CG'GwC_CG
Cuts at: 2484 2484
Size: 4289
SacI G_AGCT'C
Cuts at: 1303 1303
Size: 4289
SalI G'TCGA_C
Cuts at: 1326 1326
Size: 4289
SapI GCTCTTCn'nnn_
Cuts at: 2312 2522 2312
Size: 210 4079
SbfI CC_TGCA'GG
Cuts at: 725 1336 725
Size: 611 3678
ScaI AGT'ACT
Cuts at: 3806 3806
Size: 4289
SexAI A'CCwGG_T
Cuts at: 818 818
Size: 4289
SfcI C'TryA_G
Cuts at: 33 721 1003 1078 1186 1332 2017 3010 3201
Size: 688 282 75 108 146 685 993 191
Cuts at: 3201 33
Size: 1121
Fragments arranged by size:
1121 993 688 685 282 191 146 108
75
SfoI GGC'GCC
Cuts at: 1721 1969 1721
Size: 248 4041
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
SmaI CCC'GGG
Cuts at: 1311 1311
Size: 4289
SmlI C'TyrA_G
Cuts at: 220 244 1042 1444 2359 2851 3113 220
Size: 24 798 402 915 492 262 1396
Fragments arranged by size:
1396 915 798 492 402 262 24
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4289
SphI G_CATG'C
Cuts at: 1342 1842 1342
Size: 500 3789
StyI C'CwwG_G
Cuts at: 107 281 1423 1777 107
Size: 174 1142 354 2619
Fragments arranged by size:
2619 1142 354 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 364 702 820 1872 2005 2464 2673 364
Size: 338 118 1052 133 459 209 1980
Fragments arranged by size:
1980 1052 459 338 209 133 118
TatI w'GTAC_w
Cuts at: 3804 3804
Size: 4289
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1128 1160 3806 3838 1128
Size: 32 2646 32 1579
Fragments arranged by size:
2646 1579 32 32
Tth111I GACn'n_nGTC
Cuts at: 712 2086 712
Size: 1374 2915
XbaI T'CTAG_A
Cuts at: 1320 1320
Size: 4289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 196 1784 196
Size: 1588 2701
XhoI C'TCGA_G
Cuts at: 220 220
Size: 4289
XmaI C'CCGG_G
Cuts at: 1309 1309
Size: 4289
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI