Restriction map for
pF1KB5318
(Circular) (Six-base) MAPSORT of: pF1KB5318.seq Check: 6800 from: 1 to: 4388
pF1KB5318 4388 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 821 821
Size: 4388
AccI GT'mk_AC
Cuts at: 1426 1426
Size: 4388
Acc65I G'GTAC_C
Cuts at: 1404 1404
Size: 4388
AclI AA'CG_TT
Cuts at: 4228 4228
Size: 4388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 278 293 2213 2645 2998 3660 208
Size: 70 15 1920 432 353 662 936
Fragments arranged by size:
1920 936 662 432 353 70 15
AflIII A'CryG_T
Cuts at: 1740 3540 3821 1740
Size: 1800 281 2307
Fragments arranged by size:
2307 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 876 876
Size: 4388
AlwNI CAG_nnn'CTG
Cuts at: 1285 1693 3131 1285
Size: 408 1438 2542
Fragments arranged by size:
2542 1438 408
ApaLI G'TGCA_C
Cuts at: 3226 3226
Size: 4388
ApoI r'AATT_y
Cuts at: 415 660 666 961 1061 1392 3741 3997 415
Size: 245 6 295 100 331 2349 256 806
Fragments arranged by size:
2349 806 331 295 256 245 100 6
AseI AT'TA_AT
Cuts at: 20 20
Size: 4388
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
AvaI C'yCGr_G
Cuts at: 350 1408 350
Size: 1058 3330
BamHI G'GATC_C
Cuts at: 1413 1413
Size: 4388
BanI G'GyrC_C
Cuts at: 809 1404 1818 2066 2101 809
Size: 595 414 248 35 3096
Fragments arranged by size:
3096 595 414 248 35
BanII G_rGCy'C
Cuts at: 1402 2432 1402
Size: 1030 3358
BbeI G_GCGC'C
Cuts at: 1822 2070 1822
Size: 248 4140
BciVI GTATCCnnnnn_n'
Cuts at: 2286 3342 4388 2286
Size: 1056 1046 2286
Fragments arranged by size:
2286 1056 1046
BclI T'GATC_A
Cuts at: 849 1908 849
Size: 1059 3329
BglII A'GATC_T
Cuts at: 1903 3940 1903
Size: 2037 2351
BlpI GC'TnA_GC
Cuts at: 1500 1500
Size: 4388
Bme1580I G_kGCm'C
Cuts at: 2013 2106 3230 2013
Size: 93 1124 3171
Fragments arranged by size:
3171 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 227 1600 1681 2016 4160 227
Size: 1373 81 335 2144 455
Fragments arranged by size:
2144 1373 455 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 4388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1178 1387 1178
Size: 209 4179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1564 2443 3157 3455 1564
Size: 879 714 298 2497
Fragments arranged by size:
2497 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 655 1085 4092 655
Size: 430 3007 951
Fragments arranged by size:
3007 951 430
BsaAI yAC'GTr
Cuts at: 1741 1741
Size: 4388
BsaBI GATnn'nnATC
Cuts at: 6 1902 3939 6
Size: 1896 2037 455
Fragments arranged by size:
2037 1896 455
BsaHI Gr'CG_yC
Cuts at: 1819 2067 2769 4265 1819
Size: 248 702 1496 1942
Fragments arranged by size:
1942 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1577 2098 2776 3187 3334 3608 1577
Size: 521 678 411 147 274 2357
Fragments arranged by size:
2357 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 157
Size: 4388
BseYI C'CCAG_C
Cuts at: 306 1813 2571 3236 3644 306
Size: 1507 758 665 408 1050
Fragments arranged by size:
1507 1050 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 802
Size: 4388
BsiEI CG_ry'CG
Cuts at: 104 1930 1976 3206 104
Size: 1826 46 1230 1286
Fragments arranged by size:
1826 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 495 1402 2180 2370 3230 495
Size: 907 778 190 860 1653
Fragments arranged by size:
1653 907 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 495 1402 2013 2106 2180 2370 2432 3230 495
Size: 907 611 93 74 190 62 798 1653
Fragments arranged by size:
1653 907 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2820 2820
Size: 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 821 1438 1652 1954 2335 821
Size: 617 214 302 381 2874
Fragments arranged by size:
2874 617 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2680 2734 4218 4383 50
Size: 2630 54 1484 165 55
Fragments arranged by size:
2630 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 521 2300 521
Size: 1779 2609
BsrFI r'CCGG_y
Cuts at: 976 2386 2567 976
Size: 1410 181 2797
Fragments arranged by size:
2797 1410 181
BssSI C'ACGA_G
Cuts at: 820 2659 3367 820
Size: 1839 708 1841
Fragments arranged by size:
1841 1839 708
BstAPI GCAn_nnn'nTGC
Cuts at: 816 1177 1765 816
Size: 361 588 3439
Fragments arranged by size:
3439 588 361
BstBI TT'CG_AA
Cuts at: 1043 2749 1043
Size: 1706 2682
BstEII G'GTnAC_C
Cuts at: 628 628
Size: 4388
BstYI r'GATC_y
Cuts at: 1 835 1008 1378 1413 1880 1903 2238 2484
Size: 834 173 370 35 467 23 335 246
Cuts at: 2484 2790 2802 2888 3940 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 834 467 449 370 335 306 246
173 86 35 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4388
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2338 2519 2682 2338
Size: 181 163 4044
Fragments arranged by size:
4044 181 163
BtsI GCAGTG_nn'
Cuts at: 920 1223 1631 920
Size: 303 408 3677
Fragments arranged by size:
3677 408 303
DraI TTT'AAA
Cuts at: 1387 1387
Size: 4388
DraIII CAC_nnn'GTG
Cuts at: 909 909
Size: 4388
DrdI GACnn_nn'nnGTC
Cuts at: 318 2094 3438 318
Size: 1776 1344 1268
Fragments arranged by size:
1776 1344 1268
EaeI y'GGCC_r
Cuts at: 777 1973 2147 2538 2565 3910 777
Size: 1196 174 391 27 1345 1255
Fragments arranged by size:
1345 1255 1196 391 174 27
EagI C'GGCC_G
Cuts at: 1973 1973
Size: 4388
EarI CTCTTCn'nnn_
Cuts at: 227 1334 2411 2621 227
Size: 1107 1077 210 1994
Fragments arranged by size:
1994 1107 1077 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2596 3338 3484 4198 2596
Size: 742 146 714 2786
Fragments arranged by size:
2786 742 714 146
EcoICRI GAG'CTC
Cuts at: 1400 1400
Size: 4388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 278 293 1178 1387 2213 2645 2998 3660
Size: 70 15 885 209 826 432 353 662
Cuts at: 3660 208
Size: 936
Fragments arranged by size:
936 885 826 662 432 353 209 70
15
EcoO109I rG'GnC_Cy
Cuts at: 1527 1527
Size: 4388
EcoRI G'AATT_C
Cuts at: 660 1392 660
Size: 732 3656
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4388
FspI TGC'GCA
Cuts at: 917 2169 917
Size: 1252 3136
FspAI rTGC'GCAy
Cuts at: 917 917
Size: 4388
HaeII r_GCGC'y
Cuts at: 1822 2070 3300 4071 1822
Size: 248 1230 771 2139
Fragments arranged by size:
2139 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 204 236 663 695 862 894 1327 1359 2230
Size: 32 427 32 167 32 433 32 871
Cuts at: 2230 2260 2262 2292 204
Size: 30 2 30 2300
Fragments arranged by size:
2300 871 433 427 167 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 933 1427 933
Size: 494 3894
KasI G'GCGC_C
Cuts at: 1818 2066 1818
Size: 248 4140
KpnI G_GTAC'C
Cuts at: 1408 1408
Size: 4388
MluI A'CGCG_T
Cuts at: 3821 3821
Size: 4388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3147 3331 3147
Size: 184 4204
MscI TGG'CCA
Cuts at: 779 2149 779
Size: 1370 3018
MslI CAynn'nnrTG
Cuts at: 1055 2377 2504 1055
Size: 1322 127 2939
Fragments arranged by size:
2939 1322 127
MspA1I CmG'CkG
Cuts at: 787 1499 1813 2173 2957 3202 787
Size: 712 314 360 784 245 1973
Fragments arranged by size:
1973 784 712 360 314 245
NaeI GCC'GGC
Cuts at: 2569 2569
Size: 4388
NarI GG'CG_CC
Cuts at: 1819 2067 1819
Size: 248 4140
NcoI C'CATG_G
Cuts at: 107 107
Size: 4388
NgoMIV G'CCGG_C
Cuts at: 2567 2567
Size: 4388
NspI r_CATG'y
Cuts at: 504 1441 1941 3544 504
Size: 937 500 1603 1348
Fragments arranged by size:
1603 1348 937 500
PciI A'CATG_T
Cuts at: 3540 3540
Size: 4388
PfoI T'CCnGG_A
Cuts at: 655 655
Size: 4388
PmeI GTTT'AAAC
Cuts at: 1387 1387
Size: 4388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2834 2866 2834
Size: 32 4356
PspXI vC'TCGA_Gb
Cuts at: 350 350
Size: 4388
PstI C_TGCA'G
Cuts at: 1435 2120 1435
Size: 685 3703
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4388
PvuII CAG'CTG
Cuts at: 787 1813 2173 787
Size: 1026 360 3002
Fragments arranged by size:
3002 1026 360
RsrII CG'GwC_CG
Cuts at: 2583 2583
Size: 4388
SacI G_AGCT'C
Cuts at: 1402 1402
Size: 4388
SalI G'TCGA_C
Cuts at: 1425 1425
Size: 4388
SapI GCTCTTCn'nnn_
Cuts at: 227 2411 2621 227
Size: 2184 210 1994
Fragments arranged by size:
2184 1994 210
SbfI CC_TGCA'GG
Cuts at: 1435 1435
Size: 4388
ScaI AGT'ACT
Cuts at: 3905 3905
Size: 4388
SexAI A'CCwGG_T
Cuts at: 203 874 203
Size: 671 3717
SfcI C'TryA_G
Cuts at: 33 954 1431 2116 3084 3275 33
Size: 921 477 685 968 191 1146
Fragments arranged by size:
1146 968 921 685 477 191
SfoI GGC'GCC
Cuts at: 1820 2068 1820
Size: 248 4140
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
SmaI CCC'GGG
Cuts at: 1410 1410
Size: 4388
SmlI C'TyrA_G
Cuts at: 350 1543 2458 3172 3434 350
Size: 1193 915 714 262 1304
Fragments arranged by size:
1304 1193 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4388
SphI G_CATG'C
Cuts at: 1441 1941 1441
Size: 500 3888
SspI AAT'ATT
Cuts at: 1326 1326
Size: 4388
StyI C'CwwG_G
Cuts at: 107 1264 1522 1876 107
Size: 1157 258 354 2619
Fragments arranged by size:
2619 1157 354 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1971 2104 2563 2772 1971
Size: 133 459 209 3587
Fragments arranged by size:
3587 459 209 133
TatI w'GTAC_w
Cuts at: 354 3903 354
Size: 3549 839
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3905 3937 3905
Size: 32 4356
Tth111I GACn'n_nGTC
Cuts at: 2185 2185
Size: 4388
XbaI T'CTAG_A
Cuts at: 1419 1419
Size: 4388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1883 1883
Size: 4388
XhoI C'TCGA_G
Cuts at: 350 350
Size: 4388
XmaI C'CCGG_G
Cuts at: 1408 1408
Size: 4388
XmnI GAAnn'nnTTC
Cuts at: 965 1097 965
Size: 132 4256
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI ZraI