Restriction map for
pF1KB5324
(Circular) (Six-base) MAPSORT of: pF1KB5324.seq Check: 8630 from: 1 to: 4493
pF1KB5324 4493 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 135 135
Size: 4493
AccI GT'mk_AC
Cuts at: 1531 1531
Size: 4493
Acc65I G'GTAC_C
Cuts at: 331 1509 331
Size: 1178 3315
AclI AA'CG_TT
Cuts at: 4333 4333
Size: 4493
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 766 2318 2750 3103 3765 101
Size: 665 1552 432 353 662 829
Fragments arranged by size:
1552 829 665 662 432 353
AflIII A'CryG_T
Cuts at: 1845 3645 3926 1845
Size: 1800 281 2412
Fragments arranged by size:
2412 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 606 850 1798 3236 606
Size: 244 948 1438 1863
Fragments arranged by size:
1863 1438 948 244
ApaI G_GGCC'C
Cuts at: 582 903 582
Size: 321 4172
ApaLI G'TGCA_C
Cuts at: 720 1462 3331 720
Size: 742 1869 1882
Fragments arranged by size:
1882 1869 742
ApoI r'AATT_y
Cuts at: 1497 3846 4102 1497
Size: 2349 256 1888
Fragments arranged by size:
2349 1888 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4493
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4493
AvaI C'yCGr_G
Cuts at: 1033 1294 1513 1033
Size: 261 219 4013
Fragments arranged by size:
4013 261 219
BamHI G'GATC_C
Cuts at: 1518 1518
Size: 4493
BanI G'GyrC_C
Cuts at: 156 331 1509 1923 2171 2206 156
Size: 175 1178 414 248 35 2443
Fragments arranged by size:
2443 1178 414 248 175 35
BanII G_rGCy'C
Cuts at: 275 384 582 790 859 903 1072 1507 2537
Size: 109 198 208 69 44 169 435 1030
Cuts at: 2537 275
Size: 2231
Fragments arranged by size:
2231 1030 435 208 198 169 109 69
44
BbeI G_GCGC'C
Cuts at: 1927 2175 1927
Size: 248 4245
BbsI GAAGACnn'nnnn_
Cuts at: 1171 1171
Size: 4493
BbvCI CC'TCA_GC
Cuts at: 851 851
Size: 4493
BciVI GTATCCnnnnn_n'
Cuts at: 2391 3447 4493 2391
Size: 1056 1046 2391
Fragments arranged by size:
2391 1056 1046
BclI T'GATC_A
Cuts at: 1010 2013 1010
Size: 1003 3490
BglII A'GATC_T
Cuts at: 2008 4045 2008
Size: 2037 2456
BlpI GC'TnA_GC
Cuts at: 301 1605 301
Size: 1304 3189
Bme1580I G_kGCm'C
Cuts at: 391 582 724 903 1466 2118 2211 3335 391
Size: 191 142 179 563 652 93 1124 1549
Fragments arranged by size:
1549 1124 652 563 191 179 142 93
BmrI ACTGGGnnnn_n'
Cuts at: 387 1705 1786 2121 4265 387
Size: 1318 81 335 2144 615
Fragments arranged by size:
2144 1318 615 335 81
BmtI G_CTAG'C
Cuts at: 220 631 220
Size: 411 4082
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1014 2860 1014
Size: 1846 2647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 857 857
Size: 4493
Bpu10I CC'TnA_GC
Cuts at: 499 851 499
Size: 352 4141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 1669 2548 3262 3560 259
Size: 1410 879 714 298 1192
Fragments arranged by size:
1410 1192 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 842 1291 4197 842
Size: 449 2906 1138
Fragments arranged by size:
2906 1138 449
BsaAI yAC'GTr
Cuts at: 359 1846 359
Size: 1487 3006
BsaBI GATnn'nnATC
Cuts at: 6 2007 4044 6
Size: 2001 2037 455
Fragments arranged by size:
2037 2001 455
BsaHI Gr'CG_yC
Cuts at: 796 1924 2172 2874 4370 796
Size: 1128 248 702 1496 919
Fragments arranged by size:
1496 1128 919 702 248
BsaWI w'CCGG_w
Cuts at: 1682 2203 2881 3292 3439 3713 1682
Size: 521 678 411 147 274 2462
Fragments arranged by size:
2462 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 940 970 940
Size: 30 4463
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 860 1003 860
Size: 143 4350
BseYI C'CCAG_C
Cuts at: 582 631 659 1311 1415 1918 2676 3341 3749
Size: 49 28 652 104 503 758 665 408
Cuts at: 3749 582
Size: 1326
Fragments arranged by size:
1326 758 665 652 503 408 104 49
28
BsiEI CG_ry'CG
Cuts at: 104 2035 2081 3311 104
Size: 1931 46 1230 1286
Fragments arranged by size:
1931 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 275 575 724 765 1072 1466 1507 2285 2475
Size: 300 149 41 307 394 41 778 190
Cuts at: 2475 3335 275
Size: 860 1433
Fragments arranged by size:
1433 860 778 394 307 300 190 149
41 41
BsmBI CGTCTCn'nnnn_
Cuts at: 787 1419 787
Size: 632 3861
Bsp1286I G_dGCh'C
Cuts at: 275 384 391 575 582 724 765 790 859
Size: 109 7 184 7 142 41 25 69
Cuts at: 859 903 1072 1466 1507 2118 2211 2285 2475
Size: 44 169 394 41 611 93 74 190
Cuts at: 2475 2537 3335 275
Size: 62 798 1433
Fragments arranged by size:
1433 798 611 394 190 184 169 142
109 93 74 69 62 44 41 41
25 7 7
BspHI T'CATG_A
Cuts at: 446 2925 446
Size: 2479 2014
BspMI ACCTGCnnnn'nnnn_
Cuts at: 135 245 371 1543 1757 2059 2440 135
Size: 110 126 1172 214 302 381 2188
Fragments arranged by size:
2188 1172 381 302 214 126 110
BsrBI CCG'CTC
Cuts at: 50 618 2785 2839 4323 4488 50
Size: 568 2167 54 1484 165 55
Fragments arranged by size:
2167 1484 568 165 55 54
BsrDI GCAATG_nn'
Cuts at: 441 2405 441
Size: 1964 2529
BsrFI r'CCGG_y
Cuts at: 2491 2672 2491
Size: 181 4312
BssSI C'ACGA_G
Cuts at: 2764 3472 2764
Size: 708 3785
BstAPI GCAn_nnn'nTGC
Cuts at: 1870 1870
Size: 4493
BstBI TT'CG_AA
Cuts at: 2854 2854
Size: 4493
BstXI CCAn_nnnn'nTGG
Cuts at: 1221 1221
Size: 4493
BstYI r'GATC_y
Cuts at: 1 1439 1518 1985 2008 2343 2589 2895 2907
Size: 1438 79 467 23 335 246 306 12
Cuts at: 2907 2993 4045 1
Size: 86 1052 449
Fragments arranged by size:
1438 1052 467 449 335 306 246 86
79 23 12
BtgI C'CryG_G
Cuts at: 200 686 959 1063 1137 200
Size: 486 273 104 74 3556
Fragments arranged by size:
3556 486 273 104 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2443 2624 2787 2443
Size: 181 163 4149
Fragments arranged by size:
4149 181 163
BtsI GCAGTG_nn'
Cuts at: 643 1736 643
Size: 1093 3400
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 792 827 792
Size: 35 4458
DraI TTT'AAA
Cuts at: 1492 1492
Size: 4493
DrdI GACnn_nn'nnGTC
Cuts at: 2199 3543 2199
Size: 1344 3149
EaeI y'GGCC_r
Cuts at: 428 1154 2078 2252 2643 2670 4015 428
Size: 726 924 174 391 27 1345 906
Fragments arranged by size:
1345 924 906 726 391 174 27
EagI C'GGCC_G
Cuts at: 2078 2078
Size: 4493
EarI CTCTTCn'nnn_
Cuts at: 598 932 938 2516 2726 598
Size: 334 6 1578 210 2365
Fragments arranged by size:
2365 1578 334 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2701 3443 3589 4303 2701
Size: 742 146 714 2891
Fragments arranged by size:
2891 742 714 146
EcoICRI GAG'CTC
Cuts at: 273 1070 1505 273
Size: 797 435 3261
Fragments arranged by size:
3261 797 435
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 766 857 2318 2750 3103 3765 101
Size: 665 91 1461 432 353 662 829
Fragments arranged by size:
1461 829 665 662 432 353 91
EcoO109I rG'GnC_Cy
Cuts at: 169 579 900 1044 1632 169
Size: 410 321 144 588 3030
Fragments arranged by size:
3030 588 410 321 144
EcoRI G'AATT_C
Cuts at: 1497 1497
Size: 4493
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2512 2512
Size: 4493
FspI TGC'GCA
Cuts at: 2274 2274
Size: 4493
HaeII r_GCGC'y
Cuts at: 1452 1927 2175 3405 4176 1452
Size: 475 248 1230 771 1769
Fragments arranged by size:
1769 1230 771 475 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 940 972 2335 2365 2367 2397 940
Size: 32 1363 30 2 30 3036
Fragments arranged by size:
3036 1363 32 30 30 2
HincII GTy'rAC
Cuts at: 264 1335 1532 264
Size: 1071 197 3225
Fragments arranged by size:
3225 1071 197
KasI G'GCGC_C
Cuts at: 1923 2171 1923
Size: 248 4245
KpnI G_GTAC'C
Cuts at: 335 1513 335
Size: 1178 3315
MluI A'CGCG_T
Cuts at: 3926 3926
Size: 4493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3252 3436 3252
Size: 184 4309
MscI TGG'CCA
Cuts at: 430 1156 2254 430
Size: 726 1098 2669
Fragments arranged by size:
2669 1098 726
MslI CAynn'nnrTG
Cuts at: 685 715 1258 2482 2609 685
Size: 30 543 1224 127 2569
Fragments arranged by size:
2569 1224 543 127 30
MspA1I CmG'CkG
Cuts at: 881 926 1152 1415 1604 1918 2278 3062 3307
Size: 45 226 263 189 314 360 784 245
Cuts at: 3307 881
Size: 2067
Fragments arranged by size:
2067 784 360 314 263 245 226 189
45
NaeI GCC'GGC
Cuts at: 2674 2674
Size: 4493
NarI GG'CG_CC
Cuts at: 1924 2172 1924
Size: 248 4245
NcoI C'CATG_G
Cuts at: 686 959 686
Size: 273 4220
NgoMIV G'CCGG_C
Cuts at: 2672 2672
Size: 4493
NheI G'CTAG_C
Cuts at: 216 627 216
Size: 411 4082
NspI r_CATG'y
Cuts at: 1546 2046 3649 1546
Size: 500 1603 2390
Fragments arranged by size:
2390 1603 500
PciI A'CATG_T
Cuts at: 3645 3645
Size: 4493
PfoI T'CCnGG_A
Cuts at: 1382 1382
Size: 4493
PmeI GTTT'AAAC
Cuts at: 1492 1492
Size: 4493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2939 2971 2939
Size: 32 4461
PpuMI rG'GwC_Cy
Cuts at: 169 169
Size: 4493
PspOMI G'GGCC_C
Cuts at: 578 899 578
Size: 321 4172
PstI C_TGCA'G
Cuts at: 917 1540 2225 917
Size: 623 685 3185
Fragments arranged by size:
3185 685 623
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4493
PvuII CAG'CTG
Cuts at: 926 1152 1918 2278 926
Size: 226 766 360 3141
Fragments arranged by size:
3141 766 360 226
RsrII CG'GwC_CG
Cuts at: 2688 2688
Size: 4493
SacI G_AGCT'C
Cuts at: 275 1072 1507 275
Size: 797 435 3261
Fragments arranged by size:
3261 797 435
SalI G'TCGA_C
Cuts at: 1530 1530
Size: 4493
SanDI GG'GwC_CC
Cuts at: 169 169
Size: 4493
SapI GCTCTTCn'nnn_
Cuts at: 2516 2726 2516
Size: 210 4283
SbfI CC_TGCA'GG
Cuts at: 1540 1540
Size: 4493
ScaI AGT'ACT
Cuts at: 4010 4010
Size: 4493
SexAI A'CCwGG_T
Cuts at: 605 605
Size: 4493
SfcI C'TryA_G
Cuts at: 33 913 921 1536 2221 3189 3380 33
Size: 880 8 615 685 968 191 1146
Fragments arranged by size:
1146 968 880 685 615 191 8
SfoI GGC'GCC
Cuts at: 1925 2173 1925
Size: 248 4245
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4493
SmaI CCC'GGG
Cuts at: 1515 1515
Size: 4493
SmlI C'TyrA_G
Cuts at: 274 1648 2563 3277 3539 274
Size: 1374 915 714 262 1228
Fragments arranged by size:
1374 1228 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4493
SphI G_CATG'C
Cuts at: 1546 2046 1546
Size: 500 3993
StuI AGG'CCT
Cuts at: 919 919
Size: 4493
StyI C'CwwG_G
Cuts at: 686 959 1627 1981 686
Size: 273 668 354 3198
Fragments arranged by size:
3198 668 354 273
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2076 2209 2668 2877 2076
Size: 133 459 209 3692
Fragments arranged by size:
3692 459 209 133
TatI w'GTAC_w
Cuts at: 4008 4008
Size: 4493
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1194 1226 4010 4042 1194
Size: 32 2784 32 1645
Fragments arranged by size:
2784 1645 32 32
Tth111I GACn'n_nGTC
Cuts at: 2290 2290
Size: 4493
XbaI T'CTAG_A
Cuts at: 1524 1524
Size: 4493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1135 1988 1135
Size: 853 3640
XmaI C'CCGG_G
Cuts at: 1513 1513
Size: 4493
XmnI GAAnn'nnTTC
Cuts at: 1168 1168
Size: 4493
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BsgI
BsiWI BsmI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI