Restriction map for
pF1KB5329
(Circular) (Six-base) MAPSORT of: pF1KB5329.seq Check: 4782 from: 1 to: 3611
pF1KB5329 3611 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 649 649
Size: 3611
Acc65I G'GTAC_C
Cuts at: 627 627
Size: 3611
AclI AA'CG_TT
Cuts at: 146 3451 146
Size: 3305 306
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1436 1868 2615 2883 1436
Size: 432 747 268 2164
Fragments arranged by size:
2164 747 432 268
AflIII A'CryG_T
Cuts at: 963 2067 3044 963
Size: 1104 977 1530
Fragments arranged by size:
1530 1104 977
AgeI A'CCGG_T
Cuts at: 1999 1999
Size: 3611
AlwNI CAG_nnn'CTG
Cuts at: 916 2483 916
Size: 1567 2044
ApaLI G'TGCA_C
Cuts at: 2381 2381
Size: 3611
ApoI r'AATT_y
Cuts at: 262 379 395 615 2964 3220 262
Size: 117 16 220 2349 256 653
Fragments arranged by size:
2349 653 256 220 117 16
AseI AT'TA_AT
Cuts at: 20 20
Size: 3611
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3611
AvaI C'yCGr_G
Cuts at: 631 631
Size: 3611
BamHI G'GATC_C
Cuts at: 636 636
Size: 3611
BanI G'GyrC_C
Cuts at: 627 1041 1289 1324 627
Size: 414 248 35 2914
Fragments arranged by size:
2914 414 248 35
BanII G_rGCy'C
Cuts at: 625 1655 625
Size: 1030 2581
BbeI G_GCGC'C
Cuts at: 1045 1293 1045
Size: 248 3363
BciVI GTATCCnnnnn_n'
Cuts at: 1509 2270 3611 1509
Size: 761 1341 1509
Fragments arranged by size:
1509 1341 761
BclI T'GATC_A
Cuts at: 1131 1131
Size: 3611
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3611
BglII A'GATC_T
Cuts at: 1126 3163 1126
Size: 2037 1574
BlpI GC'TnA_GC
Cuts at: 723 723
Size: 3611
Bme1580I G_kGCm'C
Cuts at: 1236 1329 2385 1236
Size: 93 1056 2462
Fragments arranged by size:
2462 1056 93
BmgBI CAC'GTC
Cuts at: 576 576
Size: 3611
BmrI ACTGGGnnnn_n'
Cuts at: 459 823 904 1239 3383 459
Size: 364 81 335 2144 687
Fragments arranged by size:
2144 687 364 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1978 1978
Size: 3611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 202
Size: 3611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 1666 2158 2456 787
Size: 879 492 298 1942
Fragments arranged by size:
1942 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 151 3315 151
Size: 3164 447
BsaAI yAC'GTr
Cuts at: 964 964
Size: 3611
BsaBI GATnn'nnATC
Cuts at: 6 1125 3162 6
Size: 1119 2037 455
Fragments arranged by size:
2037 1119 455
BsaHI Gr'CG_yC
Cuts at: 1042 1290 1992 3488 1042
Size: 248 702 1496 1165
Fragments arranged by size:
1496 1165 702 248
BsaWI w'CCGG_w
Cuts at: 800 1321 1999 2273 2420 2831 800
Size: 521 678 274 147 411 1580
Fragments arranged by size:
1580 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1036 1794 2371 2867 1036
Size: 758 577 496 1780
Fragments arranged by size:
1780 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1153 1199 2407 104
Size: 1049 46 1208 1308
Fragments arranged by size:
1308 1208 1049 46
BsiHKAI G_wGCw'C
Cuts at: 625 1403 1593 2385 625
Size: 778 190 792 1851
Fragments arranged by size:
1851 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 625 1236 1329 1403 1593 1655 2385 625
Size: 611 93 74 190 62 730 1851
Fragments arranged by size:
1851 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2831 2831
Size: 3611
BspHI T'CATG_A
Cuts at: 287 2787 287
Size: 2500 1111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 661 875 1177 1558 661
Size: 214 302 381 2714
Fragments arranged by size:
2714 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1903 1957 3441 3606 50
Size: 1853 54 1484 165 55
Fragments arranged by size:
1853 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 410 1523 410
Size: 1113 2498
BsrFI r'CCGG_y
Cuts at: 1609 1790 1999 1609
Size: 181 209 3221
Fragments arranged by size:
3221 209 181
BssSI C'ACGA_G
Cuts at: 1882 2240 1882
Size: 358 3253
BstAPI GCAn_nnn'nTGC
Cuts at: 988 988
Size: 3611
BstBI TT'CG_AA
Cuts at: 1972 1972
Size: 3611
BstEII G'GTnAC_C
Cuts at: 301 301
Size: 3611
BstXI CCAn_nnnn'nTGG
Cuts at: 131 131
Size: 3611
BstYI r'GATC_y
Cuts at: 1 636 1103 1126 1461 1707 2719 2805 2817
Size: 635 467 23 335 246 1012 86 12
Cuts at: 2817 3163 1
Size: 346 449
Fragments arranged by size:
1012 635 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3611
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1561 1742 1905 1561
Size: 181 163 3267
Fragments arranged by size:
3267 181 163
BtsI GCAGTG_nn'
Cuts at: 854 854
Size: 3611
DraI TTT'AAA
Cuts at: 610 610
Size: 3611
DraIII CAC_nnn'GTG
Cuts at: 467 467
Size: 3611
DrdI GACnn_nn'nnGTC
Cuts at: 1317 2175 1317
Size: 858 2753
EaeI y'GGCC_r
Cuts at: 407 495 1196 1370 1761 1788 3133 407
Size: 88 701 174 391 27 1345 885
Fragments arranged by size:
1345 885 701 391 174 88 27
EagI C'GGCC_G
Cuts at: 1196 1196
Size: 3611
EarI CTCTTCn'nnn_
Cuts at: 1634 1844 1634
Size: 210 3401
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1819 2129 2275 3421 1819
Size: 310 146 1146 2009
Fragments arranged by size:
2009 1146 310 146
EcoICRI GAG'CTC
Cuts at: 623 623
Size: 3611
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 1436 1868 2615 2883 202
Size: 1234 432 747 268 930
Fragments arranged by size:
1234 930 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 750 750
Size: 3611
EcoRI G'AATT_C
Cuts at: 395 615 395
Size: 220 3391
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 379 1630 379
Size: 1251 2360
FspI TGC'GCA
Cuts at: 1392 1392
Size: 3611
HaeII r_GCGC'y
Cuts at: 1045 1293 2315 3294 1045
Size: 248 1022 979 1362
Fragments arranged by size:
1362 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1453 1483 1485 1515 1453
Size: 30 2 30 3549
Fragments arranged by size:
3549 30 30 2
HincII GTy'rAC
Cuts at: 650 650
Size: 3611
KasI G'GCGC_C
Cuts at: 1041 1289 1041
Size: 248 3363
KpnI G_GTAC'C
Cuts at: 631 631
Size: 3611
MluI A'CGCG_T
Cuts at: 3044 3044
Size: 3611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2282 2466 2282
Size: 184 3427
MscI TGG'CCA
Cuts at: 409 1372 409
Size: 963 2648
MslI CAynn'nnrTG
Cuts at: 517 1600 1727 517
Size: 1083 127 2401
Fragments arranged by size:
2401 1083 127
MspA1I CmG'CkG
Cuts at: 722 1036 1396 2409 2654 722
Size: 314 360 1013 245 1679
Fragments arranged by size:
1679 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1792 1792
Size: 3611
NarI GG'CG_CC
Cuts at: 1042 1290 1042
Size: 248 3363
NcoI C'CATG_G
Cuts at: 107 107
Size: 3611
NgoMIV G'CCGG_C
Cuts at: 1790 1790
Size: 3611
NspI r_CATG'y
Cuts at: 202 664 1164 2071 202
Size: 462 500 907 1742
Fragments arranged by size:
1742 907 500 462
PciI A'CATG_T
Cuts at: 2067 2067
Size: 3611
PflMI CCAn_nnn'nTGG
Cuts at: 467 467
Size: 3611
PmeI GTTT'AAAC
Cuts at: 610 610
Size: 3611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2750 2782 2750
Size: 32 3579
PstI C_TGCA'G
Cuts at: 658 1343 658
Size: 685 2926
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3611
PvuII CAG'CTG
Cuts at: 1036 1396 1036
Size: 360 3251
RsrII CG'GwC_CG
Cuts at: 1806 1806
Size: 3611
SacI G_AGCT'C
Cuts at: 625 625
Size: 3611
SalI G'TCGA_C
Cuts at: 648 648
Size: 3611
SapI GCTCTTCn'nnn_
Cuts at: 1634 1844 1634
Size: 210 3401
SbfI CC_TGCA'GG
Cuts at: 658 658
Size: 3611
ScaI AGT'ACT
Cuts at: 3128 3128
Size: 3611
SfcI C'TryA_G
Cuts at: 33 654 1339 2332 2523 33
Size: 621 685 993 191 1121
Fragments arranged by size:
1121 993 685 621 191
SfoI GGC'GCC
Cuts at: 1043 1291 1043
Size: 248 3363
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3611
SmaI CCC'GGG
Cuts at: 633 633
Size: 3611
SmlI C'TyrA_G
Cuts at: 766 1681 2173 2435 766
Size: 915 492 262 1942
Fragments arranged by size:
1942 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3611
SphI G_CATG'C
Cuts at: 202 664 1164 202
Size: 462 500 2649
Fragments arranged by size:
2649 500 462
StyI C'CwwG_G
Cuts at: 107 294 745 1099 107
Size: 187 451 354 2619
Fragments arranged by size:
2619 451 354 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 156 478 1194 1327 1786 1995 156
Size: 322 716 133 459 209 1772
Fragments arranged by size:
1772 716 459 322 209 133
TatI w'GTAC_w
Cuts at: 188 3126 188
Size: 2938 673
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 161 193 3128 3160 161
Size: 32 2935 32 612
Fragments arranged by size:
2935 612 32 32
Tth111I GACn'n_nGTC
Cuts at: 1408 1408
Size: 3611
XbaI T'CTAG_A
Cuts at: 642 642
Size: 3611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 150 1106 150
Size: 956 2655
XmaI C'CCGG_G
Cuts at: 631 631
Size: 3611
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI