Restriction map for
pF1KB5339
(Circular) (Six-base) MAPSORT of: pF1KB5339.seq Check: 8542 from: 1 to: 4715
pF1KB5339 4715 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 562 562
Size: 4715
AccI GT'mk_AC
Cuts at: 1753 1753
Size: 4715
Acc65I G'GTAC_C
Cuts at: 339 1731 339
Size: 1392 3323
AclI AA'CG_TT
Cuts at: 4555 4555
Size: 4715
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 860 1354 1673 2540 2972 3325 3987 653
Size: 207 494 319 867 432 353 662 1381
Fragments arranged by size:
1381 867 662 494 432 353 319 207
AflIII A'CryG_T
Cuts at: 474 2067 3867 4148 474
Size: 1593 1800 281 1041
Fragments arranged by size:
1800 1593 1041 281
AleI CACnn'nnGTG
Cuts at: 868 868
Size: 4715
AlwNI CAG_nnn'CTG
Cuts at: 1248 2020 3458 1248
Size: 772 1438 2505
Fragments arranged by size:
2505 1438 772
ApaLI G'TGCA_C
Cuts at: 3553 3553
Size: 4715
ApoI r'AATT_y
Cuts at: 516 535 616 1174 1393 1558 1719 4068 4324
Size: 19 81 558 219 165 161 2349 256
Cuts at: 4324 516
Size: 907
Fragments arranged by size:
2349 907 558 256 219 165 161 81
19
AseI AT'TA_AT
Cuts at: 20 20
Size: 4715
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4715
AvaI C'yCGr_G
Cuts at: 1735 1735
Size: 4715
BamHI G'GATC_C
Cuts at: 1740 1740
Size: 4715
BanI G'GyrC_C
Cuts at: 114 339 1731 2145 2393 2428 114
Size: 225 1392 414 248 35 2401
Fragments arranged by size:
2401 1392 414 248 225 35
BanII G_rGCy'C
Cuts at: 744 1729 2759 744
Size: 985 1030 2700
Fragments arranged by size:
2700 1030 985
BbeI G_GCGC'C
Cuts at: 118 2149 2397 118
Size: 2031 248 2436
Fragments arranged by size:
2436 2031 248
BciVI GTATCCnnnnn_n'
Cuts at: 2613 3669 4715 2613
Size: 1056 1046 2613
Fragments arranged by size:
2613 1056 1046
BclI T'GATC_A
Cuts at: 2235 2235
Size: 4715
BfrBI ATG'CAT
Cuts at: 882 882
Size: 4715
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4715
BglII A'GATC_T
Cuts at: 2230 4267 2230
Size: 2037 2678
BlpI GC'TnA_GC
Cuts at: 432 1827 432
Size: 1395 3320
Bme1580I G_kGCm'C
Cuts at: 2340 2433 3557 2340
Size: 93 1124 3498
Fragments arranged by size:
3498 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 415 1927 2008 2343 4487 415
Size: 1512 81 335 2144 643
Fragments arranged by size:
2144 1512 643 335 81
BmtI G_CTAG'C
Cuts at: 724 724
Size: 4715
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 316 3082 316
Size: 2766 1949
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1891 2770 3484 3782 1891
Size: 879 714 298 2824
Fragments arranged by size:
2824 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4419 4419
Size: 4715
BsaAI yAC'GTr
Cuts at: 2068 2068
Size: 4715
BsaBI GATnn'nnATC
Cuts at: 6 2229 4266 6
Size: 2223 2037 455
Fragments arranged by size:
2223 2037 455
BsaHI Gr'CG_yC
Cuts at: 115 130 1264 2146 2394 3096 4592 115
Size: 15 1134 882 248 702 1496 238
Fragments arranged by size:
1496 1134 882 702 248 238 15
BsaWI w'CCGG_w
Cuts at: 464 1904 2425 3103 3514 3661 3935 464
Size: 1440 521 678 411 147 274 1244
Fragments arranged by size:
1440 1244 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1298 1328 1298
Size: 30 4685
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 1434 226
Size: 1208 3507
BseYI C'CCAG_C
Cuts at: 777 1587 2140 2898 3563 3971 777
Size: 810 553 758 665 408 1521
Fragments arranged by size:
1521 810 758 665 553 408
BsiEI CG_ry'CG
Cuts at: 104 2257 2303 3533 104
Size: 2153 46 1230 1286
Fragments arranged by size:
2153 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 433 464 1729 2507 2697 3557 433
Size: 31 1265 778 190 860 1591
Fragments arranged by size:
1591 1265 860 778 190 31
BsmI GAATG_Cn'
Cuts at: 651 879 651
Size: 228 4487
BsmBI CGTCTCn'nnnn_
Cuts at: 121 121
Size: 4715
Bsp1286I G_dGCh'C
Cuts at: 433 464 744 1729 2340 2433 2507 2697 2759
Size: 31 280 985 611 93 74 190 62
Cuts at: 2759 3557 433
Size: 798 1591
Fragments arranged by size:
1591 985 798 611 280 190 93 74
62 31
BspHI T'CATG_A
Cuts at: 3147 3147
Size: 4715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 562 1765 1979 2281 2662 562
Size: 1203 214 302 381 2615
Fragments arranged by size:
2615 1203 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3007 3061 4545 4710 50
Size: 2957 54 1484 165 55
Fragments arranged by size:
2957 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1592 2627 1592
Size: 1035 3680
BsrFI r'CCGG_y
Cuts at: 2713 2894 2713
Size: 181 4534
BssHII G'CGCG_C
Cuts at: 183 183
Size: 4715
BssSI C'ACGA_G
Cuts at: 2986 3694 2986
Size: 708 4007
BstAPI GCAn_nnn'nTGC
Cuts at: 691 2092 691
Size: 1401 3314
BstBI TT'CG_AA
Cuts at: 614 3076 614
Size: 2462 2253
BstYI r'GATC_y
Cuts at: 1 1740 2207 2230 2565 2811 3117 3129 3215
Size: 1739 467 23 335 246 306 12 86
Cuts at: 3215 4267 1
Size: 1052 449
Fragments arranged by size:
1739 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1526 1526
Size: 4715
BtgI C'CryG_G
Cuts at: 107 122 107
Size: 15 4700
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 578 892 2665 2846 3009 578
Size: 314 1773 181 163 2284
Fragments arranged by size:
2284 1773 314 181 163
BtsI GCAGTG_nn'
Cuts at: 1233 1389 1455 1958 1233
Size: 156 66 503 3990
Fragments arranged by size:
3990 503 156 66
DraI TTT'AAA
Cuts at: 621 1172 1683 1714 621
Size: 551 511 31 3622
Fragments arranged by size:
3622 551 511 31
DrdI GACnn_nn'nnGTC
Cuts at: 2421 3765 2421
Size: 1344 3371
EaeI y'GGCC_r
Cuts at: 1230 2300 2474 2865 2892 4237 1230
Size: 1070 174 391 27 1345 1708
Fragments arranged by size:
1708 1345 1070 391 174 27
EagI C'GGCC_G
Cuts at: 2300 2300
Size: 4715
EarI CTCTTCn'nnn_
Cuts at: 836 1469 2738 2948 836
Size: 633 1269 210 2603
Fragments arranged by size:
2603 1269 633 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2923 3665 3811 4525 2923
Size: 742 146 714 3113
Fragments arranged by size:
3113 742 714 146
EcoICRI GAG'CTC
Cuts at: 1727 1727
Size: 4715
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 860 1354 1673 2540 2972 3325 3987 653
Size: 207 494 319 867 432 353 662 1381
Fragments arranged by size:
1381 867 662 494 432 353 319 207
EcoO109I rG'GnC_Cy
Cuts at: 1854 1854
Size: 4715
EcoRI G'AATT_C
Cuts at: 535 1719 535
Size: 1184 3531
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4715
FspI TGC'GCA
Cuts at: 2496 2496
Size: 4715
HaeII r_GCGC'y
Cuts at: 118 2149 2397 3627 4398 118
Size: 2031 248 1230 771 435
Fragments arranged by size:
2031 1230 771 435 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 307 339 691 723 817 849 1298 1330 2557
Size: 32 352 32 94 32 449 32 1227
Cuts at: 2557 2587 2589 2619 307
Size: 30 2 30 2403
Fragments arranged by size:
2403 1227 449 352 94 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 585 1754 585
Size: 1169 3546
KasI G'GCGC_C
Cuts at: 114 2145 2393 114
Size: 2031 248 2436
Fragments arranged by size:
2436 2031 248
KpnI G_GTAC'C
Cuts at: 343 1735 343
Size: 1392 3323
MluI A'CGCG_T
Cuts at: 4148 4148
Size: 4715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1065 1622 3474 3658 1065
Size: 557 1852 184 2122
Fragments arranged by size:
2122 1852 557 184
MscI TGG'CCA
Cuts at: 1232 2476 1232
Size: 1244 3471
MslI CAynn'nnrTG
Cuts at: 386 868 917 1001 2704 2831 386
Size: 482 49 84 1703 127 2270
Fragments arranged by size:
2270 1703 482 127 84 49
MspA1I CmG'CkG
Cuts at: 1430 1826 2140 2500 3284 3529 1430
Size: 396 314 360 784 245 2616
Fragments arranged by size:
2616 784 396 360 314 245
NaeI GCC'GGC
Cuts at: 2896 2896
Size: 4715
NarI GG'CG_CC
Cuts at: 115 2146 2394 115
Size: 2031 248 2436
Fragments arranged by size:
2436 2031 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4715
NgoMIV G'CCGG_C
Cuts at: 2894 2894
Size: 4715
NheI G'CTAG_C
Cuts at: 720 720
Size: 4715
NsiI A_TGCA'T
Cuts at: 884 884
Size: 4715
NspI r_CATG'y
Cuts at: 478 1768 2268 3871 478
Size: 1290 500 1603 1322
Fragments arranged by size:
1603 1322 1290 500
PciI A'CATG_T
Cuts at: 474 3867 474
Size: 3393 1322
PflMI CCAn_nnn'nTGG
Cuts at: 943 943
Size: 4715
PmeI GTTT'AAAC
Cuts at: 1714 1714
Size: 4715
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 4683
PsiI TTA'TAA
Cuts at: 329 329
Size: 4715
PstI C_TGCA'G
Cuts at: 573 1272 1762 2447 573
Size: 699 490 685 2841
Fragments arranged by size:
2841 699 685 490
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4715
PvuII CAG'CTG
Cuts at: 1430 2140 2500 1430
Size: 710 360 3645
Fragments arranged by size:
3645 710 360
RsrII CG'GwC_CG
Cuts at: 2910 2910
Size: 4715
SacI G_AGCT'C
Cuts at: 1729 1729
Size: 4715
SalI G'TCGA_C
Cuts at: 1752 1752
Size: 4715
SapI GCTCTTCn'nnn_
Cuts at: 2738 2948 2738
Size: 210 4505
SbfI CC_TGCA'GG
Cuts at: 1762 1762
Size: 4715
ScaI AGT'ACT
Cuts at: 4232 4232
Size: 4715
SfcI C'TryA_G
Cuts at: 33 446 569 1268 1758 2443 3411 3602 33
Size: 413 123 699 490 685 968 191 1146
Fragments arranged by size:
1146 968 699 685 490 413 191 123
SfoI GGC'GCC
Cuts at: 116 2147 2395 116
Size: 2031 248 2436
Fragments arranged by size:
2436 2031 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4715
SmaI CCC'GGG
Cuts at: 1737 1737
Size: 4715
SmlI C'TyrA_G
Cuts at: 1870 2785 3499 3761 1870
Size: 915 714 262 2824
Fragments arranged by size:
2824 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1372 67
Size: 1305 3410
SphI G_CATG'C
Cuts at: 1768 2268 1768
Size: 500 4215
StyI C'CwwG_G
Cuts at: 107 710 1849 2203 107
Size: 603 1139 354 2619
Fragments arranged by size:
2619 1139 603 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 769 2298 2431 2890 3099 769
Size: 1529 133 459 209 2385
Fragments arranged by size:
2385 1529 459 209 133
TatI w'GTAC_w
Cuts at: 4230 4230
Size: 4715
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4232 4264 4232
Size: 32 4683
Tth111I GACn'n_nGTC
Cuts at: 2512 2512
Size: 4715
XbaI T'CTAG_A
Cuts at: 1746 1746
Size: 4715
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2210 2210
Size: 4715
XmaI C'CCGG_G
Cuts at: 1735 1735
Size: 4715
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NsiI NspI PciI PflMI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BmgBI BpmI
Bpu10I BsgI BsiWI BspEI BsrGI BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NotI NruI PacI PasI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI