Restriction map for
pF1KB5341
(Circular) (Six-base) MAPSORT of: pF1KB5341.seq Check: 3229 from: 1 to: 4151
pF1KB5341 4151 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1189 1189
Size: 4151
Acc65I G'GTAC_C
Cuts at: 1167 1167
Size: 4151
AclI AA'CG_TT
Cuts at: 3991 3991
Size: 4151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 826 1976 2408 2761 3423 404
Size: 422 1150 432 353 662 1132
Fragments arranged by size:
1150 1132 662 432 422 353
AflIII A'CryG_T
Cuts at: 1503 3303 3584 1503
Size: 1800 281 2070
Fragments arranged by size:
2070 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 989 1456 2894 989
Size: 467 1438 2246
Fragments arranged by size:
2246 1438 467
ApaI G_GGCC'C
Cuts at: 683 683
Size: 4151
ApaLI G'TGCA_C
Cuts at: 2989 2989
Size: 4151
ApoI r'AATT_y
Cuts at: 1155 3504 3760 1155
Size: 2349 256 1546
Fragments arranged by size:
2349 1546 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4151
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 218 1171 218
Size: 953 3198
BamHI G'GATC_C
Cuts at: 1176 1176
Size: 4151
BanI G'GyrC_C
Cuts at: 1167 1581 1829 1864 1167
Size: 414 248 35 3454
Fragments arranged by size:
3454 414 248 35
BanII G_rGCy'C
Cuts at: 342 405 683 1165 2195 342
Size: 63 278 482 1030 2298
Fragments arranged by size:
2298 1030 482 278 63
BbeI G_GCGC'C
Cuts at: 1585 1833 1585
Size: 248 3903
BbsI GAAGACnn'nnnn_
Cuts at: 735 735
Size: 4151
BciVI GTATCCnnnnn_n'
Cuts at: 589 2049 3105 4151 589
Size: 1460 1056 1046 589
Fragments arranged by size:
1460 1056 1046 589
BclI T'GATC_A
Cuts at: 1671 1671
Size: 4151
BglII A'GATC_T
Cuts at: 539 1666 3703 539
Size: 1127 2037 987
Fragments arranged by size:
2037 1127 987
BlpI GC'TnA_GC
Cuts at: 1263 1263
Size: 4151
Bme1580I G_kGCm'C
Cuts at: 683 1776 1869 2993 683
Size: 1093 93 1124 1841
Fragments arranged by size:
1841 1124 1093 93
BmrI ACTGGGnnnn_n'
Cuts at: 368 872 955 1003 1363 1444 1779 3923 368
Size: 504 83 48 360 81 335 2144 596
Fragments arranged by size:
2144 596 504 360 335 83 81 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 308 495 787 798 901 211
Size: 97 187 292 11 103 3461
Fragments arranged by size:
3461 292 187 103 97 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1327 2206 2920 3218 1327
Size: 879 714 298 2260
Fragments arranged by size:
2260 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3855 3855
Size: 4151
BsaAI yAC'GTr
Cuts at: 1504 1504
Size: 4151
BsaBI GATnn'nnATC
Cuts at: 6 1665 3702 6
Size: 1659 2037 455
Fragments arranged by size:
2037 1659 455
BsaHI Gr'CG_yC
Cuts at: 1582 1830 2532 4028 1582
Size: 248 702 1496 1705
Fragments arranged by size:
1705 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1340 1861 2539 2950 3097 3371 1340
Size: 521 678 411 147 274 2120
Fragments arranged by size:
2120 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 979 1009 979
Size: 30 4121
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 160 163 649 1067 136
Size: 24 3 486 418 3220
Fragments arranged by size:
3220 486 418 24 3
BseYI C'CCAG_C
Cuts at: 1576 2334 2999 3407 1576
Size: 758 665 408 2320
Fragments arranged by size:
2320 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1693 1739 2969 104
Size: 1589 46 1230 1286
Fragments arranged by size:
1589 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 342 405 845 1165 1943 2133 2993 342
Size: 63 440 320 778 190 860 1500
Fragments arranged by size:
1500 860 778 440 320 190 63
Bsp1286I G_dGCh'C
Cuts at: 342 405 683 845 1165 1776 1869 1943 2133
Size: 63 278 162 320 611 93 74 190
Cuts at: 2133 2195 2993 342
Size: 62 798 1500
Fragments arranged by size:
1500 798 611 320 278 190 162 93
74 63 62
BspHI T'CATG_A
Cuts at: 2583 2583
Size: 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 971 1201 1415 1717 2098 971
Size: 230 214 302 381 3024
Fragments arranged by size:
3024 381 302 230 214
BsrBI CCG'CTC
Cuts at: 50 2443 2497 3981 4146 50
Size: 2393 54 1484 165 55
Fragments arranged by size:
2393 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2063 2063
Size: 4151
BsrFI r'CCGG_y
Cuts at: 167 2149 2330 167
Size: 1982 181 1988
Fragments arranged by size:
1988 1982 181
BssSI C'ACGA_G
Cuts at: 2422 3130 2422
Size: 708 3443
BstAPI GCAn_nnn'nTGC
Cuts at: 799 1528 799
Size: 729 3422
BstBI TT'CG_AA
Cuts at: 2512 2512
Size: 4151
BstYI r'GATC_y
Cuts at: 1 173 330 539 873 1176 1643 1666 2001
Size: 172 157 209 334 303 467 23 335
Cuts at: 2001 2247 2553 2565 2651 3703 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 467 449 335 334 306 303 246
209 172 157 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 971 971
Size: 4151
BtgI C'CryG_G
Cuts at: 107 419 591 107
Size: 312 172 3667
Fragments arranged by size:
3667 312 172
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2101 2282 2445 2101
Size: 181 163 3807
Fragments arranged by size:
3807 181 163
BtsI GCAGTG_nn'
Cuts at: 789 1394 789
Size: 605 3546
DraI TTT'AAA
Cuts at: 1150 1150
Size: 4151
DrdI GACnn_nn'nnGTC
Cuts at: 1857 3201 1857
Size: 1344 2807
EaeI y'GGCC_r
Cuts at: 369 1736 1910 2301 2328 3673 369
Size: 1367 174 391 27 1345 847
Fragments arranged by size:
1367 1345 847 391 174 27
EagI C'GGCC_G
Cuts at: 1736 1736
Size: 4151
EarI CTCTTCn'nnn_
Cuts at: 864 2174 2384 864
Size: 1310 210 2631
Fragments arranged by size:
2631 1310 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 166 2359 3101 3247 3961 166
Size: 2193 742 146 714 356
Fragments arranged by size:
2193 742 714 356 146
EcoICRI GAG'CTC
Cuts at: 340 403 1163 340
Size: 63 760 3328
Fragments arranged by size:
3328 760 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 308 404 495 787 798 826 901 1976
Size: 97 96 91 292 11 28 75 1075
Cuts at: 1976 2408 2761 3423 211
Size: 432 353 662 939
Fragments arranged by size:
1075 939 662 432 353 292 97 96
91 75 28 11
EcoO109I rG'GnC_Cy
Cuts at: 1056 1071 1290 1056
Size: 15 219 3917
Fragments arranged by size:
3917 219 15
EcoRI G'AATT_C
Cuts at: 1155 1155
Size: 4151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 759 2170 759
Size: 1411 2740
FspI TGC'GCA
Cuts at: 1932 1932
Size: 4151
HaeII r_GCGC'y
Cuts at: 1585 1833 3063 3834 1585
Size: 248 1230 771 1902
Fragments arranged by size:
1902 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1993 2023 2025 2055 1993
Size: 30 2 30 4089
Fragments arranged by size:
4089 30 30 2
HincII GTy'rAC
Cuts at: 534 1190 534
Size: 656 3495
KasI G'GCGC_C
Cuts at: 1581 1829 1581
Size: 248 3903
KpnI G_GTAC'C
Cuts at: 1171 1171
Size: 4151
MluI A'CGCG_T
Cuts at: 3584 3584
Size: 4151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 845 1005 2910 3094 845
Size: 160 1905 184 1902
Fragments arranged by size:
1905 1902 184 160
MscI TGG'CCA
Cuts at: 1912 1912
Size: 4151
MslI CAynn'nnrTG
Cuts at: 2140 2267 2140
Size: 127 4024
MspA1I CmG'CkG
Cuts at: 1068 1262 1576 1936 2720 2965 1068
Size: 194 314 360 784 245 2254
Fragments arranged by size:
2254 784 360 314 245 194
NaeI GCC'GGC
Cuts at: 2332 2332
Size: 4151
NarI GG'CG_CC
Cuts at: 1582 1830 1582
Size: 248 3903
NcoI C'CATG_G
Cuts at: 107 419 591 107
Size: 312 172 3667
Fragments arranged by size:
3667 312 172
NdeI CA'TA_TG
Cuts at: 891 891
Size: 4151
NgoMIV G'CCGG_C
Cuts at: 2330 2330
Size: 4151
NspI r_CATG'y
Cuts at: 1204 1704 3307 1204
Size: 500 1603 2048
Fragments arranged by size:
2048 1603 500
PciI A'CATG_T
Cuts at: 3303 3303
Size: 4151
PfoI T'CCnGG_A
Cuts at: 333 333
Size: 4151
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2597 2629 2597
Size: 32 4119
PpuMI rG'GwC_Cy
Cuts at: 1071 1071
Size: 4151
PspOMI G'GGCC_C
Cuts at: 679 679
Size: 4151
PstI C_TGCA'G
Cuts at: 1198 1883 1198
Size: 685 3466
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4151
PvuII CAG'CTG
Cuts at: 1576 1936 1576
Size: 360 3791
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 4151
SacI G_AGCT'C
Cuts at: 342 405 1165 342
Size: 63 760 3328
Fragments arranged by size:
3328 760 63
SalI G'TCGA_C
Cuts at: 1188 1188
Size: 4151
SanDI GG'GwC_CC
Cuts at: 1071 1071
Size: 4151
SapI GCTCTTCn'nnn_
Cuts at: 2174 2384 2174
Size: 210 3941
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 3668 3668
Size: 4151
SfcI C'TryA_G
Cuts at: 33 1194 1879 2847 3038 33
Size: 1161 685 968 191 1146
Fragments arranged by size:
1161 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1583 1831 1583
Size: 248 3903
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 1173 1173
Size: 4151
SmlI C'TyrA_G
Cuts at: 1306 2221 2935 3197 1306
Size: 915 714 262 2260
Fragments arranged by size:
2260 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 1204 1704 1204
Size: 500 3651
StyI C'CwwG_G
Cuts at: 107 419 591 663 1134 1142 1285 1639 107
Size: 312 172 72 471 8 143 354 2619
Fragments arranged by size:
2619 471 354 312 172 143 72 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1734 1867 2326 2535 1734
Size: 133 459 209 3350
Fragments arranged by size:
3350 459 209 133
TatI w'GTAC_w
Cuts at: 3666 3666
Size: 4151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3668 3700 3668
Size: 32 4119
Tth111I GACn'n_nGTC
Cuts at: 979 1948 979
Size: 969 3182
XbaI T'CTAG_A
Cuts at: 1182 1182
Size: 4151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1646 1646
Size: 4151
XmaI C'CCGG_G
Cuts at: 1171 1171
Size: 4151
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI