Restriction map for
pF1KB5343
(Circular) (Six-base) MAPSORT of: pF1KB5343.seq Check: 2246 from: 1 to: 4877
pF1KB5343 4877 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1265 1265
Size: 4877
AatII G_ACGT'C
Cuts at: 1211 1211
Size: 4877
AccI GT'mk_AC
Cuts at: 1241 1915 1241
Size: 674 4203
Acc65I G'GTAC_C
Cuts at: 1893 1893
Size: 4877
AclI AA'CG_TT
Cuts at: 295 4717 295
Size: 4422 455
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1117 1447 2702 3134 3487 4149 247
Size: 870 330 1255 432 353 662 975
Fragments arranged by size:
1255 975 870 662 432 353 330
AflIII A'CryG_T
Cuts at: 902 2229 4029 4310 902
Size: 1327 1800 281 1469
Fragments arranged by size:
1800 1469 1327 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1394 1426 1394
Size: 32 4845
AlwNI CAG_nnn'CTG
Cuts at: 1564 1677 2182 3620 1564
Size: 113 505 1438 2821
Fragments arranged by size:
2821 1438 505 113
ApaLI G'TGCA_C
Cuts at: 3715 3715
Size: 4877
ApoI r'AATT_y
Cuts at: 1881 4230 4486 1881
Size: 2349 256 2272
Fragments arranged by size:
2349 2272 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4877
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4877
AvaI C'yCGr_G
Cuts at: 856 1897 856
Size: 1041 3836
BamHI G'GATC_C
Cuts at: 1902 1902
Size: 4877
BanI G'GyrC_C
Cuts at: 895 969 1119 1648 1893 2307 2555 2590 895
Size: 74 150 529 245 414 248 35 3182
Fragments arranged by size:
3182 529 414 248 245 150 74 35
BanII G_rGCy'C
Cuts at: 1891 2921 1891
Size: 1030 3847
BbeI G_GCGC'C
Cuts at: 2311 2559 2311
Size: 248 4629
BbsI GAAGACnn'nnnn_
Cuts at: 642 1046 642
Size: 404 4473
BciVI GTATCCnnnnn_n'
Cuts at: 488 2775 3831 4877 488
Size: 2287 1056 1046 488
Fragments arranged by size:
2287 1056 1046 488
BclI T'GATC_A
Cuts at: 2397 2397
Size: 4877
BglI GCCn_nnn'nGGC
Cuts at: 955 1594 1858 955
Size: 639 264 3974
Fragments arranged by size:
3974 639 264
BglII A'GATC_T
Cuts at: 2392 4429 2392
Size: 2037 2840
BlpI GC'TnA_GC
Cuts at: 193 1989 193
Size: 1796 3081
Bme1580I G_kGCm'C
Cuts at: 639 898 1124 2502 2595 3719 639
Size: 259 226 1378 93 1124 1797
Fragments arranged by size:
1797 1378 1124 259 226 93
BmgBI CAC'GTC
Cuts at: 750 750
Size: 4877
BmrI ACTGGGnnnn_n'
Cuts at: 700 1287 2089 2170 2505 4649 700
Size: 587 802 81 335 2144 928
Fragments arranged by size:
2144 928 802 587 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1365 3244 1365
Size: 1879 2998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 1078 1681 172
Size: 906 603 3368
Fragments arranged by size:
3368 906 603
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 2053 2932 3646 3944 139
Size: 1914 879 714 298 1072
Fragments arranged by size:
1914 1072 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4581 4581
Size: 4877
BsaAI yAC'GTr
Cuts at: 570 1284 2230 570
Size: 714 946 3217
Fragments arranged by size:
3217 946 714
BsaBI GATnn'nnATC
Cuts at: 6 2391 4428 6
Size: 2385 2037 455
Fragments arranged by size:
2385 2037 455
BsaHI Gr'CG_yC
Cuts at: 1208 2308 2556 3258 4754 1208
Size: 1100 248 702 1496 1331
Fragments arranged by size:
1496 1331 1100 702 248
BsaWI w'CCGG_w
Cuts at: 374 383 2066 2587 3265 3676 3823 4097 374
Size: 9 1683 521 678 411 147 274 1154
Fragments arranged by size:
1683 1154 678 521 411 274 147 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1143 1173 1394 1424 1143
Size: 30 221 30 4596
Fragments arranged by size:
4596 221 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 316 997 1000 1498 1501 1555 1558 1561 1702
Size: 681 3 498 3 54 3 3 141
Cuts at: 1702 1705 316
Size: 3 3488
Fragments arranged by size:
3488 681 498 141 54 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 798 2302 3060 3725 4133 798
Size: 1504 758 665 408 1542
Fragments arranged by size:
1542 1504 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 1306 1378 592
Size: 714 72 4091
Fragments arranged by size:
4091 714 72
BsiEI CG_ry'CG
Cuts at: 104 1245 2419 2465 3695 104
Size: 1141 1174 46 1230 1286
Fragments arranged by size:
1286 1230 1174 1141 46
BsiHKAI G_wGCw'C
Cuts at: 1891 2669 2859 3719 1891
Size: 778 190 860 3049
Fragments arranged by size:
3049 860 778 190
BsmI GAATG_Cn'
Cuts at: 221 221
Size: 4877
Bsp1286I G_dGCh'C
Cuts at: 639 898 1124 1891 2502 2595 2669 2859 2921
Size: 259 226 767 611 93 74 190 62
Cuts at: 2921 3719 639
Size: 798 1797
Fragments arranged by size:
1797 798 767 611 259 226 190 93
74 62
BspEI T'CCGG_A
Cuts at: 374 383 374
Size: 9 4868
BspHI T'CATG_A
Cuts at: 3309 3309
Size: 4877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1265 1927 2141 2443 2824 1265
Size: 662 214 302 381 3318
Fragments arranged by size:
3318 662 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3169 3223 4707 4872 50
Size: 3119 54 1484 165 55
Fragments arranged by size:
3119 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2789 2789
Size: 4877
BsrFI r'CCGG_y
Cuts at: 593 886 1244 2875 3056 593
Size: 293 358 1631 181 2414
Fragments arranged by size:
2414 1631 358 293 181
BssSI C'ACGA_G
Cuts at: 3148 3856 3148
Size: 708 4169
BstAPI GCAn_nnn'nTGC
Cuts at: 2254 2254
Size: 4877
BstBI TT'CG_AA
Cuts at: 3238 3238
Size: 4877
BstXI CCAn_nnnn'nTGG
Cuts at: 798 1641 1710 1800 798
Size: 843 69 90 3875
Fragments arranged by size:
3875 843 90 69
BstYI r'GATC_y
Cuts at: 1 1902 2369 2392 2727 2973 3279 3291 3377
Size: 1901 467 23 335 246 306 12 86
Cuts at: 3377 4429 1
Size: 1052 449
Fragments arranged by size:
1901 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 550 1334 107
Size: 443 784 3650
Fragments arranged by size:
3650 784 443
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2827 3008 3171 2827
Size: 181 163 4533
Fragments arranged by size:
4533 181 163
BtsI GCAGTG_nn'
Cuts at: 2120 2120
Size: 4877
ClaI AT'CG_AT
Cuts at: 491 491
Size: 4877
DraI TTT'AAA
Cuts at: 428 1876 428
Size: 1448 3429
DrdI GACnn_nn'nnGTC
Cuts at: 760 2583 3927 760
Size: 1823 1344 1710
Fragments arranged by size:
1823 1710 1344
EaeI y'GGCC_r
Cuts at: 146 767 1712 2462 2636 3027 3054 4399 146
Size: 621 945 750 174 391 27 1345 624
Fragments arranged by size:
1345 945 750 624 621 391 174 27
EagI C'GGCC_G
Cuts at: 2462 2462
Size: 4877
EarI CTCTTCn'nnn_
Cuts at: 394 1475 1487 1520 1577 1601 2900 3110 394
Size: 1081 12 33 57 24 1299 210 2161
Fragments arranged by size:
2161 1299 1081 210 57 33 24 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 805 1819 3085 3827 3973 4687 805
Size: 1014 1266 742 146 714 995
Fragments arranged by size:
1266 1014 995 742 714 146
EcoICRI GAG'CTC
Cuts at: 1889 1889
Size: 4877
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 247 1078 1117 1447 1681 2702 3134 3487
Size: 75 831 39 330 234 1021 432 353
Cuts at: 3487 4149 172
Size: 662 900
Fragments arranged by size:
1021 900 831 662 432 353 330 234
75 39
EcoNI CCTnn'n_nnAGG
Cuts at: 643 643
Size: 4877
EcoO109I rG'GnC_Cy
Cuts at: 504 1092 1131 1455 2016 504
Size: 588 39 324 561 3365
Fragments arranged by size:
3365 588 561 324 39
EcoRI G'AATT_C
Cuts at: 1881 1881
Size: 4877
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2896 2896
Size: 4877
FspI TGC'GCA
Cuts at: 643 2658 643
Size: 2015 2862
HaeII r_GCGC'y
Cuts at: 1710 2311 2559 3789 4560 1710
Size: 601 248 1230 771 2027
Fragments arranged by size:
2027 1230 771 601 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 505 537 2719 2749 2751 2781 505
Size: 32 2182 30 2 30 2601
Fragments arranged by size:
2601 2182 32 30 30 2
HincII GTy'rAC
Cuts at: 270 345 810 1242 1916 270
Size: 75 465 432 674 3231
Fragments arranged by size:
3231 674 465 432 75
HindIII A'AGCT_T
Cuts at: 1081 1081
Size: 4877
HpaI GTT'AAC
Cuts at: 270 270
Size: 4877
KasI G'GCGC_C
Cuts at: 2307 2555 2307
Size: 248 4629
KpnI G_GTAC'C
Cuts at: 1897 1897
Size: 4877
MluI A'CGCG_T
Cuts at: 902 4310 902
Size: 3408 1469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 887 3636 3820 887
Size: 2749 184 1944
Fragments arranged by size:
2749 1944 184
MscI TGG'CCA
Cuts at: 769 1714 2638 769
Size: 945 924 3008
Fragments arranged by size:
3008 945 924
MslI CAynn'nnrTG
Cuts at: 1720 2866 2993 1720
Size: 1146 127 3604
Fragments arranged by size:
3604 1146 127
MspA1I CmG'CkG
Cuts at: 192 552 585 1362 1446 1674 1988 2302 2662
Size: 360 33 777 84 228 314 314 360
Cuts at: 2662 3446 3691 192
Size: 784 245 1378
Fragments arranged by size:
1378 784 777 360 360 314 314 245
228 84 33
NaeI GCC'GGC
Cuts at: 595 3058 595
Size: 2463 2414
NarI GG'CG_CC
Cuts at: 2308 2556 2308
Size: 248 4629
NcoI C'CATG_G
Cuts at: 107 107
Size: 4877
NgoMIV G'CCGG_C
Cuts at: 593 3056 593
Size: 2463 2414
NspI r_CATG'y
Cuts at: 1930 2430 4033 1930
Size: 500 1603 2774
Fragments arranged by size:
2774 1603 500
PasI CC'CwG_GG
Cuts at: 1634 1634
Size: 4877
PciI A'CATG_T
Cuts at: 4029 4029
Size: 4877
PflMI CCAn_nnn'nTGG
Cuts at: 707 707
Size: 4877
PmeI GTTT'AAAC
Cuts at: 1876 1876
Size: 4877
PmlI CAC'GTG
Cuts at: 570 570
Size: 4877
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1394 1426 3323 3355 1394
Size: 32 1897 32 2916
Fragments arranged by size:
2916 1897 32 32
PpuMI rG'GwC_Cy
Cuts at: 504 1455 504
Size: 951 3926
PshAI GACnn'nnGTC
Cuts at: 1264 1264
Size: 4877
PspXI vC'TCGA_Gb
Cuts at: 856 856
Size: 4877
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 469 501 469
Size: 32 4845
PstI C_TGCA'G
Cuts at: 1445 1924 2609 1445
Size: 479 685 3713
Fragments arranged by size:
3713 685 479
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4877
PvuII CAG'CTG
Cuts at: 192 585 1362 2302 2662 192
Size: 393 777 940 360 2407
Fragments arranged by size:
2407 940 777 393 360
RsrII CG'GwC_CG
Cuts at: 3072 3072
Size: 4877
SacI G_AGCT'C
Cuts at: 1891 1891
Size: 4877
SacII CC_GC'GG
Cuts at: 553 553
Size: 4877
SalI G'TCGA_C
Cuts at: 1240 1914 1240
Size: 674 4203
SapI GCTCTTCn'nnn_
Cuts at: 394 1601 2900 3110 394
Size: 1207 1299 210 2161
Fragments arranged by size:
2161 1299 1207 210
SbfI CC_TGCA'GG
Cuts at: 1924 1924
Size: 4877
ScaI AGT'ACT
Cuts at: 1345 4394 1345
Size: 3049 1828
SfcI C'TryA_G
Cuts at: 33 281 416 756 1441 1920 2605 3573 3764
Size: 248 135 340 685 479 685 968 191
Cuts at: 3764 33
Size: 1146
Fragments arranged by size:
1146 968 685 685 479 340 248 191
135
SfoI GGC'GCC
Cuts at: 2309 2557 2309
Size: 248 4629
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4877
SmaI CCC'GGG
Cuts at: 1899 1899
Size: 4877
SmlI C'TyrA_G
Cuts at: 118 856 2032 2947 3661 3923 118
Size: 738 1176 915 714 262 1072
Fragments arranged by size:
1176 1072 915 738 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4877
SphI G_CATG'C
Cuts at: 1930 2430 1930
Size: 500 4377
StuI AGG'CCT
Cuts at: 116 116
Size: 4877
StyI C'CwwG_G
Cuts at: 107 2011 2365 107
Size: 1904 354 2619
Fragments arranged by size:
2619 1904 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1243 1287 1405 2460 2593 3052 3261 1243
Size: 44 118 1055 133 459 209 2859
Fragments arranged by size:
2859 1055 459 209 133 118 44
TatI w'GTAC_w
Cuts at: 1343 4392 1343
Size: 3049 1828
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1143 1175 4394 4426 1143
Size: 32 3219 32 1594
Fragments arranged by size:
3219 1594 32 32
Tth111I GACn'n_nGTC
Cuts at: 2674 2674
Size: 4877
XbaI T'CTAG_A
Cuts at: 1908 1908
Size: 4877
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2372 2372
Size: 4877
XhoI C'TCGA_G
Cuts at: 856 856
Size: 4877
XmaI C'CCGG_G
Cuts at: 1897 1897
Size: 4877
XmnI GAAnn'nnTTC
Cuts at: 1102 1102
Size: 4877
ZraI GAC'GTC
Cuts at: 1209 1209
Size: 4877
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I CspCI DraIII
EcoRV FseI FspAI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PsiI PspOMI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI