Restriction map for
pF1KB5348
(Circular) (Six-base) MAPSORT of: pF1KB5348.seq Check: 3302 from: 1 to: 4283
pF1KB5348 4283 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 384 1321 384
Size: 937 3346
Acc65I G'GTAC_C
Cuts at: 1172 1299 1172
Size: 127 4156
AclI AA'CG_TT
Cuts at: 4123 4123
Size: 4283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 2108 2540 2893 3555 593
Size: 1515 432 353 662 1321
Fragments arranged by size:
1515 1321 662 432 353
AflIII A'CryG_T
Cuts at: 1635 3435 3716 1635
Size: 1800 281 2202
Fragments arranged by size:
2202 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1588 3026 1588
Size: 1438 2845
ApaI G_GGCC'C
Cuts at: 1007 1007
Size: 4283
ApaLI G'TGCA_C
Cuts at: 3121 3121
Size: 4283
ApoI r'AATT_y
Cuts at: 516 1287 3636 3892 516
Size: 771 2349 256 907
Fragments arranged by size:
2349 907 771 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4283
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
AvaI C'yCGr_G
Cuts at: 1303 1303
Size: 4283
BamHI G'GATC_C
Cuts at: 1308 1308
Size: 4283
BanI G'GyrC_C
Cuts at: 1172 1299 1713 1961 1996 1172
Size: 127 414 248 35 3459
Fragments arranged by size:
3459 414 248 127 35
BanII G_rGCy'C
Cuts at: 651 1007 1297 2327 651
Size: 356 290 1030 2607
Fragments arranged by size:
2607 1030 356 290
BbeI G_GCGC'C
Cuts at: 1717 1965 1717
Size: 248 4035
BciVI GTATCCnnnnn_n'
Cuts at: 2181 3237 4283 2181
Size: 1056 1046 2181
Fragments arranged by size:
2181 1056 1046
BclI T'GATC_A
Cuts at: 1803 1803
Size: 4283
BglII A'GATC_T
Cuts at: 598 1798 3835 598
Size: 1200 2037 1046
Fragments arranged by size:
2037 1200 1046
BlpI GC'TnA_GC
Cuts at: 1395 1395
Size: 4283
Bme1580I G_kGCm'C
Cuts at: 1007 1908 2001 3125 1007
Size: 901 93 1124 2165
Fragments arranged by size:
2165 1124 901 93
BmrI ACTGGGnnnn_n'
Cuts at: 544 1495 1576 1911 4055 544
Size: 951 81 335 2144 772
Fragments arranged by size:
2144 951 772 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2650 2650
Size: 4283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1256 1256
Size: 4283
Bpu10I CC'TnA_GC
Cuts at: 293 293
Size: 4283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1074 1098 1459 2338 3052 3350 1074
Size: 24 361 879 714 298 2007
Fragments arranged by size:
2007 879 714 361 298 24
BsaI GGTCTCn'nnnn_
Cuts at: 3987 3987
Size: 4283
BsaAI yAC'GTr
Cuts at: 661 862 982 1057 1186 1636 661
Size: 201 120 75 129 450 3308
Fragments arranged by size:
3308 450 201 129 120 75
BsaBI GATnn'nnATC
Cuts at: 6 869 1797 3834 6
Size: 863 928 2037 455
Fragments arranged by size:
2037 928 863 455
BsaHI Gr'CG_yC
Cuts at: 1714 1962 2664 4160 1714
Size: 248 702 1496 1837
Fragments arranged by size:
1837 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1472 1993 2671 3082 3229 3503 1472
Size: 521 678 411 147 274 2252
Fragments arranged by size:
2252 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 461 497 641 461
Size: 36 144 4103
Fragments arranged by size:
4103 144 36
BseYI C'CCAG_C
Cuts at: 282 1708 2466 3131 3539 282
Size: 1426 758 665 408 1026
Fragments arranged by size:
1426 1026 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 587
Size: 4283
BsiEI CG_ry'CG
Cuts at: 104 1825 1871 3101 104
Size: 1721 46 1230 1286
Fragments arranged by size:
1721 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 651 990 1218 1297 2075 2265 3125 651
Size: 339 228 79 778 190 860 1809
Fragments arranged by size:
1809 860 778 339 228 190 79
Bsp1286I G_dGCh'C
Cuts at: 651 990 1007 1218 1297 1908 2001 2075 2265
Size: 339 17 211 79 611 93 74 190
Cuts at: 2265 2327 3125 651
Size: 62 798 1809
Fragments arranged by size:
1809 798 611 339 211 190 93 79
74 62 17
BspHI T'CATG_A
Cuts at: 2715 2715
Size: 4283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1333 1547 1849 2230 1333
Size: 214 302 381 3386
Fragments arranged by size:
3386 381 302 214
BsrBI CCG'CTC
Cuts at: 50 702 2575 2629 4113 4278 50
Size: 652 1873 54 1484 165 55
Fragments arranged by size:
1873 1484 652 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2195 2195
Size: 4283
BsrFI r'CCGG_y
Cuts at: 2281 2462 2281
Size: 181 4102
BssSI C'ACGA_G
Cuts at: 479 791 821 998 2554 3262 479
Size: 312 30 177 1556 708 1500
Fragments arranged by size:
1556 1500 708 312 177 30
BstAPI GCAn_nnn'nTGC
Cuts at: 1660 1660
Size: 4283
BstBI TT'CG_AA
Cuts at: 2644 2644
Size: 4283
BstXI CCAn_nnnn'nTGG
Cuts at: 348 348
Size: 4283
BstYI r'GATC_y
Cuts at: 1 598 1308 1775 1798 2133 2379 2685 2697
Size: 597 710 467 23 335 246 306 12
Cuts at: 2697 2783 3835 1
Size: 86 1052 449
Fragments arranged by size:
1052 710 597 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1209 107
Size: 1102 3181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2233 2414 2577 2233
Size: 181 163 3939
Fragments arranged by size:
3939 181 163
BtsI GCAGTG_nn'
Cuts at: 636 1526 636
Size: 890 3393
DraI TTT'AAA
Cuts at: 1282 1282
Size: 4283
DraIII CAC_nnn'GTG
Cuts at: 985 985
Size: 4283
DrdI GACnn_nn'nnGTC
Cuts at: 1989 3333 1989
Size: 1344 2939
EaeI y'GGCC_r
Cuts at: 1868 2042 2433 2460 3805 1868
Size: 174 391 27 1345 2346
Fragments arranged by size:
2346 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1868 1868
Size: 4283
EarI CTCTTCn'nnn_
Cuts at: 142 438 639 660 2306 2516 142
Size: 296 201 21 1646 210 1909
Fragments arranged by size:
1909 1646 296 210 201 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2491 3233 3379 4093 2491
Size: 742 146 714 2681
Fragments arranged by size:
2681 742 714 146
EcoICRI GAG'CTC
Cuts at: 649 1295 649
Size: 646 3637
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 1256 2108 2540 2893 3555 593
Size: 663 852 432 353 662 1321
Fragments arranged by size:
1321 852 663 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 425 479 425
Size: 54 4229
EcoO109I rG'GnC_Cy
Cuts at: 418 469 583 1003 1004 1422 418
Size: 51 114 420 1 418 3279
Fragments arranged by size:
3279 420 418 114 51 1
EcoRI G'AATT_C
Cuts at: 1287 1287
Size: 4283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 4283
FspI TGC'GCA
Cuts at: 2064 2064
Size: 4283
HaeII r_GCGC'y
Cuts at: 1717 1965 3195 3966 1717
Size: 248 1230 771 2034
Fragments arranged by size:
2034 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 859 891 1072 1096 1104 1128 2125 2155 2157
Size: 32 181 24 8 24 997 30 2
Cuts at: 2157 2187 859
Size: 30 2955
Fragments arranged by size:
2955 997 181 32 30 30 24 24
8 2
HincII GTy'rAC
Cuts at: 1322 1322
Size: 4283
KasI G'GCGC_C
Cuts at: 1713 1961 1713
Size: 248 4035
KpnI G_GTAC'C
Cuts at: 1176 1303 1176
Size: 127 4156
MluI A'CGCG_T
Cuts at: 3716 3716
Size: 4283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3042 3226 3042
Size: 184 4099
MscI TGG'CCA
Cuts at: 2044 2044
Size: 4283
MslI CAynn'nnrTG
Cuts at: 346 494 935 2272 2399 346
Size: 148 441 1337 127 2230
Fragments arranged by size:
2230 1337 441 148 127
MspA1I CmG'CkG
Cuts at: 1211 1394 1708 2068 2852 3097 1211
Size: 183 314 360 784 245 2397
Fragments arranged by size:
2397 784 360 314 245 183
NaeI GCC'GGC
Cuts at: 2464 2464
Size: 4283
NarI GG'CG_CC
Cuts at: 1714 1962 1714
Size: 248 4035
NcoI C'CATG_G
Cuts at: 107 107
Size: 4283
NdeI CA'TA_TG
Cuts at: 960 960
Size: 4283
NgoMIV G'CCGG_C
Cuts at: 2462 2462
Size: 4283
NspI r_CATG'y
Cuts at: 1336 1836 3439 1336
Size: 500 1603 2180
Fragments arranged by size:
2180 1603 500
PciI A'CATG_T
Cuts at: 3435 3435
Size: 4283
PflMI CCAn_nnn'nTGG
Cuts at: 577 1018 577
Size: 441 3842
PmeI GTTT'AAAC
Cuts at: 1282 1282
Size: 4283
PmlI CAC'GTG
Cuts at: 661 862 1057 1186 661
Size: 201 195 129 3758
Fragments arranged by size:
3758 201 195 129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2729 2761 2729
Size: 32 4251
PpuMI rG'GwC_Cy
Cuts at: 418 469 583 418
Size: 51 114 4118
Fragments arranged by size:
4118 114 51
PspOMI G'GGCC_C
Cuts at: 1003 1003
Size: 4283
PstI C_TGCA'G
Cuts at: 1330 2015 1330
Size: 685 3598
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4283
PvuII CAG'CTG
Cuts at: 1708 2068 1708
Size: 360 3923
RsrII CG'GwC_CG
Cuts at: 2478 2478
Size: 4283
SacI G_AGCT'C
Cuts at: 651 1297 651
Size: 646 3637
SacII CC_GC'GG
Cuts at: 1212 1212
Size: 4283
SalI G'TCGA_C
Cuts at: 1320 1320
Size: 4283
SapI GCTCTTCn'nnn_
Cuts at: 142 639 2306 2516 142
Size: 497 1667 210 1909
Fragments arranged by size:
1909 1667 497 210
SbfI CC_TGCA'GG
Cuts at: 1330 1330
Size: 4283
ScaI AGT'ACT
Cuts at: 218 3800 218
Size: 3582 701
SfcI C'TryA_G
Cuts at: 33 948 1160 1194 1326 2011 2979 3170 33
Size: 915 212 34 132 685 968 191 1146
Fragments arranged by size:
1146 968 915 685 212 191 132 34
SfoI GGC'GCC
Cuts at: 1715 1963 1715
Size: 248 4035
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
SmaI CCC'GGG
Cuts at: 1305 1305
Size: 4283
SmlI C'TyrA_G
Cuts at: 1089 1113 1438 2353 3067 3329 1089
Size: 24 325 915 714 262 2043
Fragments arranged by size:
2043 915 714 325 262 24
SpeI A'CTAG_T
Cuts at: 67 615 67
Size: 548 3735
SphI G_CATG'C
Cuts at: 1336 1836 1336
Size: 500 3783
StyI C'CwwG_G
Cuts at: 107 1417 1771 107
Size: 1310 354 2619
Fragments arranged by size:
2619 1310 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1866 1999 2458 2667 1866
Size: 133 459 209 3482
Fragments arranged by size:
3482 459 209 133
TatI w'GTAC_w
Cuts at: 216 3798 216
Size: 3582 701
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 194 226 3800 3832 194
Size: 32 3574 32 645
Fragments arranged by size:
3574 645 32 32
Tth111I GACn'n_nGTC
Cuts at: 2080 2080
Size: 4283
XbaI T'CTAG_A
Cuts at: 1314 1314
Size: 4283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1778 1778
Size: 4283
XmaI C'CCGG_G
Cuts at: 1303 1303
Size: 4283
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PflMI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BsaXI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PasI PfoI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI