Restriction map for
pF1KB5359
(Circular) (Six-base) MAPSORT of: pF1KB5359.seq Check: 3822 from: 1 to: 3662
pF1KB5359 3662 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 700 700
Size: 3662
Acc65I G'GTAC_C
Cuts at: 678 678
Size: 3662
AclI AA'CG_TT
Cuts at: 3502 3502
Size: 3662
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 274 317 1487 1919 2666 2934 209
Size: 65 43 1170 432 747 268 937
Fragments arranged by size:
1170 937 747 432 268 65 43
AflIII A'CryG_T
Cuts at: 222 1014 2118 3095 222
Size: 792 1104 977 789
Fragments arranged by size:
1104 977 792 789
AgeI A'CCGG_T
Cuts at: 2050 2050
Size: 3662
AlwNI CAG_nnn'CTG
Cuts at: 187 277 967 2534 187
Size: 90 690 1567 1315
Fragments arranged by size:
1567 1315 690 90
ApaLI G'TGCA_C
Cuts at: 2432 2432
Size: 3662
ApoI r'AATT_y
Cuts at: 193 303 666 3015 3271 193
Size: 110 363 2349 256 584
Fragments arranged by size:
2349 584 363 256 110
AseI AT'TA_AT
Cuts at: 20 20
Size: 3662
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
AvaI C'yCGr_G
Cuts at: 682 682
Size: 3662
BamHI G'GATC_C
Cuts at: 687 687
Size: 3662
BanI G'GyrC_C
Cuts at: 490 678 1092 1340 1375 490
Size: 188 414 248 35 2777
Fragments arranged by size:
2777 414 248 188 35
BanII G_rGCy'C
Cuts at: 676 1706 676
Size: 1030 2632
BbeI G_GCGC'C
Cuts at: 1096 1344 1096
Size: 248 3414
BciVI GTATCCnnnnn_n'
Cuts at: 1560 2321 3662 1560
Size: 761 1341 1560
Fragments arranged by size:
1560 1341 761
BclI T'GATC_A
Cuts at: 1182 1182
Size: 3662
BglII A'GATC_T
Cuts at: 1177 3214 1177
Size: 2037 1625
BlpI GC'TnA_GC
Cuts at: 774 774
Size: 3662
Bme1580I G_kGCm'C
Cuts at: 1287 1380 2436 1287
Size: 93 1056 2513
Fragments arranged by size:
2513 1056 93
BmgBI CAC'GTC
Cuts at: 223 223
Size: 3662
BmrI ACTGGGnnnn_n'
Cuts at: 874 955 1290 3434 874
Size: 81 335 2144 1102
Fragments arranged by size:
2144 1102 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 3662
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 638 838 1717 2209 2507 638
Size: 200 879 492 298 1793
Fragments arranged by size:
1793 879 492 298 200
BsaI GGTCTCn'nnnn_
Cuts at: 3366 3366
Size: 3662
BsaAI yAC'GTr
Cuts at: 1015 1015
Size: 3662
BsaBI GATnn'nnATC
Cuts at: 6 1176 3213 6
Size: 1170 2037 455
Fragments arranged by size:
2037 1170 455
BsaHI Gr'CG_yC
Cuts at: 1093 1341 2043 3539 1093
Size: 248 702 1496 1216
Fragments arranged by size:
1496 1216 702 248
BsaWI w'CCGG_w
Cuts at: 851 1372 2050 2324 2471 2882 851
Size: 521 678 274 147 411 1631
Fragments arranged by size:
1631 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1087 1845 2422 2918 1087
Size: 758 577 496 1831
Fragments arranged by size:
1831 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1204 1250 2458 104
Size: 1100 46 1208 1308
Fragments arranged by size:
1308 1208 1100 46
BsiHKAI G_wGCw'C
Cuts at: 676 1454 1644 2436 676
Size: 778 190 792 1902
Fragments arranged by size:
1902 792 778 190
BsmI GAATG_Cn'
Cuts at: 630 630
Size: 3662
Bsp1286I G_dGCh'C
Cuts at: 676 1287 1380 1454 1644 1706 2436 676
Size: 611 93 74 190 62 730 1902
Fragments arranged by size:
1902 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2882 2882
Size: 3662
BspHI T'CATG_A
Cuts at: 524 2838 524
Size: 2314 1348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 712 926 1228 1609 712
Size: 214 302 381 2765
Fragments arranged by size:
2765 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1954 2008 3492 3657 50
Size: 1904 54 1484 165 55
Fragments arranged by size:
1904 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 127 1574 127
Size: 1447 2215
BsrFI r'CCGG_y
Cuts at: 1660 1841 2050 1660
Size: 181 209 3272
Fragments arranged by size:
3272 209 181
BssSI C'ACGA_G
Cuts at: 1933 2291 1933
Size: 358 3304
BstAPI GCAn_nnn'nTGC
Cuts at: 1039 1039
Size: 3662
BstBI TT'CG_AA
Cuts at: 2023 2023
Size: 3662
BstYI r'GATC_y
Cuts at: 1 687 1154 1177 1512 1758 2770 2856 2868
Size: 686 467 23 335 246 1012 86 12
Cuts at: 2868 3214 1
Size: 346 449
Fragments arranged by size:
1012 686 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3662
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1612 1793 1956 1612
Size: 181 163 3318
Fragments arranged by size:
3318 181 163
BtsI GCAGTG_nn'
Cuts at: 510 905 510
Size: 395 3267
DraI TTT'AAA
Cuts at: 661 661
Size: 3662
DrdI GACnn_nn'nnGTC
Cuts at: 1368 2226 1368
Size: 858 2804
EaeI y'GGCC_r
Cuts at: 1247 1421 1812 1839 3184 1247
Size: 174 391 27 1345 1725
Fragments arranged by size:
1725 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1247 1247
Size: 3662
EarI CTCTTCn'nnn_
Cuts at: 170 608 1685 1895 170
Size: 438 1077 210 1937
Fragments arranged by size:
1937 1077 438 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1870 2180 2326 3472 1870
Size: 310 146 1146 2060
Fragments arranged by size:
2060 1146 310 146
EcoICRI GAG'CTC
Cuts at: 674 674
Size: 3662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 274 317 1487 1919 2666 2934 209
Size: 65 43 1170 432 747 268 937
Fragments arranged by size:
1170 937 747 432 268 65 43
EcoO109I rG'GnC_Cy
Cuts at: 801 801
Size: 3662
EcoRI G'AATT_C
Cuts at: 666 666
Size: 3662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 352 401 1681 352
Size: 49 1280 2333
Fragments arranged by size:
2333 1280 49
FspI TGC'GCA
Cuts at: 1443 1443
Size: 3662
HaeII r_GCGC'y
Cuts at: 1096 1344 2366 3345 1096
Size: 248 1022 979 1413
Fragments arranged by size:
1413 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 385 400 417 432 1504 1534 1536 1566 385
Size: 15 17 15 1072 30 2 30 2481
Fragments arranged by size:
2481 1072 30 30 17 15 15 2
HincII GTy'rAC
Cuts at: 701 701
Size: 3662
KasI G'GCGC_C
Cuts at: 1092 1340 1092
Size: 248 3414
KpnI G_GTAC'C
Cuts at: 682 682
Size: 3662
MluI A'CGCG_T
Cuts at: 3095 3095
Size: 3662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 140 2333 2517 140
Size: 2193 184 1285
Fragments arranged by size:
2193 1285 184
MscI TGG'CCA
Cuts at: 1423 1423
Size: 3662
MslI CAynn'nnrTG
Cuts at: 1651 1778 1651
Size: 127 3535
MspA1I CmG'CkG
Cuts at: 773 1087 1447 2460 2705 773
Size: 314 360 1013 245 1730
Fragments arranged by size:
1730 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1843 1843
Size: 3662
NarI GG'CG_CC
Cuts at: 1093 1341 1093
Size: 248 3414
NcoI C'CATG_G
Cuts at: 107 107
Size: 3662
NgoMIV G'CCGG_C
Cuts at: 1841 1841
Size: 3662
NspI r_CATG'y
Cuts at: 715 1215 2122 715
Size: 500 907 2255
Fragments arranged by size:
2255 907 500
PciI A'CATG_T
Cuts at: 2118 2118
Size: 3662
PmeI GTTT'AAAC
Cuts at: 661 661
Size: 3662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2801 2833 2801
Size: 32 3630
PstI C_TGCA'G
Cuts at: 709 1394 709
Size: 685 2977
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3662
PvuII CAG'CTG
Cuts at: 1087 1447 1087
Size: 360 3302
RsrII CG'GwC_CG
Cuts at: 1857 1857
Size: 3662
SacI G_AGCT'C
Cuts at: 676 676
Size: 3662
SalI G'TCGA_C
Cuts at: 699 699
Size: 3662
SapI GCTCTTCn'nnn_
Cuts at: 1685 1895 1685
Size: 210 3452
SbfI CC_TGCA'GG
Cuts at: 709 709
Size: 3662
ScaI AGT'ACT
Cuts at: 212 3179 212
Size: 2967 695
SfcI C'TryA_G
Cuts at: 33 705 1390 2383 2574 33
Size: 672 685 993 191 1121
Fragments arranged by size:
1121 993 685 672 191
SfoI GGC'GCC
Cuts at: 1094 1342 1094
Size: 248 3414
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
SmaI CCC'GGG
Cuts at: 684 684
Size: 3662
SmlI C'TyrA_G
Cuts at: 653 817 1732 2224 2486 653
Size: 164 915 492 262 1829
Fragments arranged by size:
1829 915 492 262 164
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3662
SphI G_CATG'C
Cuts at: 715 1215 715
Size: 500 3162
SspI AAT'ATT
Cuts at: 247 247
Size: 3662
StyI C'CwwG_G
Cuts at: 107 796 1150 107
Size: 689 354 2619
Fragments arranged by size:
2619 689 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1245 1378 1837 2046 1245
Size: 133 459 209 2861
Fragments arranged by size:
2861 459 209 133
TatI w'GTAC_w
Cuts at: 210 3177 210
Size: 2967 695
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 3630
Tth111I GACn'n_nGTC
Cuts at: 1459 1459
Size: 3662
XbaI T'CTAG_A
Cuts at: 693 693
Size: 3662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1157 1157
Size: 3662
XmaI C'CCGG_G
Cuts at: 682 682
Size: 3662
XmnI GAAnn'nnTTC
Cuts at: 332 332
Size: 3662
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI