Restriction map for
pF1KB5363
(Circular) (Six-base) MAPSORT of: pF1KB5363.seq Check: 3983 from: 1 to: 3575
pF1KB5363 3575 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 251 613 251
Size: 362 3213
Acc65I G'GTAC_C
Cuts at: 591 591
Size: 3575
AclI AA'CG_TT
Cuts at: 3415 3415
Size: 3575
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 1400 1832 2185 2847 317
Size: 1083 432 353 662 1045
Fragments arranged by size:
1083 1045 662 432 353
AflIII A'CryG_T
Cuts at: 927 2727 3008 927
Size: 1800 281 1494
Fragments arranged by size:
1800 1494 281
AlwNI CAG_nnn'CTG
Cuts at: 880 2318 880
Size: 1438 2137
ApaLI G'TGCA_C
Cuts at: 2413 2413
Size: 3575
ApoI r'AATT_y
Cuts at: 579 2928 3184 579
Size: 2349 256 970
Fragments arranged by size:
2349 970 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3575
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3575
AvaI C'yCGr_G
Cuts at: 595 595
Size: 3575
BamHI G'GATC_C
Cuts at: 600 600
Size: 3575
BanI G'GyrC_C
Cuts at: 591 1005 1253 1288 591
Size: 414 248 35 2878
Fragments arranged by size:
2878 414 248 35
BanII G_rGCy'C
Cuts at: 589 1619 589
Size: 1030 2545
BbeI G_GCGC'C
Cuts at: 1009 1257 1009
Size: 248 3327
BciVI GTATCCnnnnn_n'
Cuts at: 1473 2529 3575 1473
Size: 1056 1046 1473
Fragments arranged by size:
1473 1056 1046
BclI T'GATC_A
Cuts at: 1095 1095
Size: 3575
BglII A'GATC_T
Cuts at: 1090 3127 1090
Size: 2037 1538
BlpI GC'TnA_GC
Cuts at: 687 687
Size: 3575
Bme1580I G_kGCm'C
Cuts at: 517 1200 1293 2417 517
Size: 683 93 1124 1675
Fragments arranged by size:
1675 1124 683 93
BmrI ACTGGGnnnn_n'
Cuts at: 787 868 1203 3347 787
Size: 81 335 2144 1015
Fragments arranged by size:
2144 1015 335 81
BmtI G_CTAG'C
Cuts at: 182 283 182
Size: 101 3474
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1942 1942
Size: 3575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 1630 2344 2642 751
Size: 879 714 298 1684
Fragments arranged by size:
1684 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3279 3279
Size: 3575
BsaAI yAC'GTr
Cuts at: 512 928 512
Size: 416 3159
BsaBI GATnn'nnATC
Cuts at: 6 1089 3126 6
Size: 1083 2037 455
Fragments arranged by size:
2037 1083 455
BsaHI Gr'CG_yC
Cuts at: 1006 1254 1956 3452 1006
Size: 248 702 1496 1129
Fragments arranged by size:
1496 1129 702 248
BsaWI w'CCGG_w
Cuts at: 764 1285 1963 2374 2521 2795 764
Size: 521 678 411 147 274 1544
Fragments arranged by size:
1544 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1000 1758 2423 2831 1000
Size: 758 665 408 1744
Fragments arranged by size:
1744 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 174 1117 1163 2393 104
Size: 70 943 46 1230 1286
Fragments arranged by size:
1286 1230 943 70 46
BsiHKAI G_wGCw'C
Cuts at: 589 1367 1557 2417 589
Size: 778 190 860 1747
Fragments arranged by size:
1747 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 517 589 1200 1293 1367 1557 1619 2417 517
Size: 72 611 93 74 190 62 798 1675
Fragments arranged by size:
1675 798 611 190 93 74 72 62
BspHI T'CATG_A
Cuts at: 2007 2007
Size: 3575
BspMI ACCTGCnnnn'nnnn_
Cuts at: 625 839 1141 1522 625
Size: 214 302 381 2678
Fragments arranged by size:
2678 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1867 1921 3405 3570 50
Size: 1817 54 1484 165 55
Fragments arranged by size:
1817 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1487 1487
Size: 3575
BsrFI r'CCGG_y
Cuts at: 1573 1754 1573
Size: 181 3394
BssSI C'ACGA_G
Cuts at: 157 1846 2554 157
Size: 1689 708 1178
Fragments arranged by size:
1689 1178 708
BstAPI GCAn_nnn'nTGC
Cuts at: 952 952
Size: 3575
BstBI TT'CG_AA
Cuts at: 1936 1936
Size: 3575
BstYI r'GATC_y
Cuts at: 1 392 502 600 1067 1090 1425 1671 1977
Size: 391 110 98 467 23 335 246 306
Cuts at: 1977 1989 2075 3127 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 467 449 391 335 306 246 110
98 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3575
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1525 1706 1869 1525
Size: 181 163 3231
Fragments arranged by size:
3231 181 163
BtsI GCAGTG_nn'
Cuts at: 544 818 544
Size: 274 3301
DraI TTT'AAA
Cuts at: 574 574
Size: 3575
DrdI GACnn_nn'nnGTC
Cuts at: 1281 2625 1281
Size: 1344 2231
EaeI y'GGCC_r
Cuts at: 1160 1334 1725 1752 3097 1160
Size: 174 391 27 1345 1638
Fragments arranged by size:
1638 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1160 1160
Size: 3575
EarI CTCTTCn'nnn_
Cuts at: 293 366 1598 1808 293
Size: 73 1232 210 2060
Fragments arranged by size:
2060 1232 210 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1783 2525 2671 3385 1783
Size: 742 146 714 1973
Fragments arranged by size:
1973 742 714 146
EcoICRI GAG'CTC
Cuts at: 587 587
Size: 3575
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 1400 1832 2185 2847 317
Size: 1083 432 353 662 1045
Fragments arranged by size:
1083 1045 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 435 714 435
Size: 279 3296
EcoRI G'AATT_C
Cuts at: 579 579
Size: 3575
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1594 1594
Size: 3575
FspI TGC'GCA
Cuts at: 1356 1356
Size: 3575
HaeII r_GCGC'y
Cuts at: 1009 1257 2487 3258 1009
Size: 248 1230 771 1326
Fragments arranged by size:
1326 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 130 151 162 183 1417 1447 1449 1479 130
Size: 21 11 21 1234 30 2 30 2226
Fragments arranged by size:
2226 1234 30 30 21 21 11 2
HincII GTy'rAC
Cuts at: 614 614
Size: 3575
KasI G'GCGC_C
Cuts at: 1005 1253 1005
Size: 248 3327
KpnI G_GTAC'C
Cuts at: 595 595
Size: 3575
MluI A'CGCG_T
Cuts at: 3008 3008
Size: 3575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 2334 2518 194
Size: 2140 184 1251
Fragments arranged by size:
2140 1251 184
MscI TGG'CCA
Cuts at: 1336 1336
Size: 3575
MslI CAynn'nnrTG
Cuts at: 1564 1691 1564
Size: 127 3448
MspA1I CmG'CkG
Cuts at: 291 686 1000 1360 2144 2389 291
Size: 395 314 360 784 245 1477
Fragments arranged by size:
1477 784 395 360 314 245
NaeI GCC'GGC
Cuts at: 1756 1756
Size: 3575
NarI GG'CG_CC
Cuts at: 1006 1254 1006
Size: 248 3327
NcoI C'CATG_G
Cuts at: 107 107
Size: 3575
NgoMIV G'CCGG_C
Cuts at: 1754 1754
Size: 3575
NheI G'CTAG_C
Cuts at: 178 279 178
Size: 101 3474
NspI r_CATG'y
Cuts at: 462 628 1128 2731 462
Size: 166 500 1603 1306
Fragments arranged by size:
1603 1306 500 166
PciI A'CATG_T
Cuts at: 2727 2727
Size: 3575
PfoI T'CCnGG_A
Cuts at: 395 395
Size: 3575
PmeI GTTT'AAAC
Cuts at: 574 574
Size: 3575
PmlI CAC'GTG
Cuts at: 512 512
Size: 3575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2021 2053 2021
Size: 32 3543
PpuMI rG'GwC_Cy
Cuts at: 435 435
Size: 3575
PstI C_TGCA'G
Cuts at: 622 1307 622
Size: 685 2890
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3575
PvuII CAG'CTG
Cuts at: 291 1000 1360 291
Size: 709 360 2506
Fragments arranged by size:
2506 709 360
RsrII CG'GwC_CG
Cuts at: 1770 1770
Size: 3575
SacI G_AGCT'C
Cuts at: 589 589
Size: 3575
SalI G'TCGA_C
Cuts at: 612 612
Size: 3575
SanDI GG'GwC_CC
Cuts at: 435 435
Size: 3575
SapI GCTCTTCn'nnn_
Cuts at: 1598 1808 1598
Size: 210 3365
SbfI CC_TGCA'GG
Cuts at: 622 622
Size: 3575
ScaI AGT'ACT
Cuts at: 3092 3092
Size: 3575
SfcI C'TryA_G
Cuts at: 33 618 1303 2271 2462 33
Size: 585 685 968 191 1146
Fragments arranged by size:
1146 968 685 585 191
SfoI GGC'GCC
Cuts at: 1007 1255 1007
Size: 248 3327
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3575
SmaI CCC'GGG
Cuts at: 597 597
Size: 3575
SmlI C'TyrA_G
Cuts at: 730 1645 2359 2621 730
Size: 915 714 262 1684
Fragments arranged by size:
1684 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3575
SphI G_CATG'C
Cuts at: 462 628 1128 462
Size: 166 500 2909
Fragments arranged by size:
2909 500 166
StyI C'CwwG_G
Cuts at: 107 709 1063 107
Size: 602 354 2619
Fragments arranged by size:
2619 602 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 272 1158 1291 1750 1959 272
Size: 886 133 459 209 1888
Fragments arranged by size:
1888 886 459 209 133
TatI w'GTAC_w
Cuts at: 3090 3090
Size: 3575
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 3543
Tth111I GACn'n_nGTC
Cuts at: 1372 1372
Size: 3575
XbaI T'CTAG_A
Cuts at: 606 606
Size: 3575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1070 1070
Size: 3575
XmaI C'CCGG_G
Cuts at: 595 595
Size: 3575
XmnI GAAnn'nnTTC
Cuts at: 236 236
Size: 3575
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BglII BlpI Bme1580I BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PciI PfoI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI
BfrBI BglI BmgBI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI
PacI PasI PflMI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI