(Circular) (Six-base) MAPSORT of: pF1KB5364.seq Check: 3732 from: 1 to: 4298 pF1KB5364 4298 bp With 182 enzymes: SgfI * October 7, 2009 14:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 381 381 Size: 4298 AccI GT'mk_AC Cuts at: 1214 1336 1214 Size: 122 4176 Acc65I G'GTAC_C Cuts at: 1314 1314 Size: 4298 AclI AA'CG_TT Cuts at: 783 4138 783 Size: 3355 943 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 206 367 2123 2555 3302 3570 206 Size: 161 1756 432 747 268 934 Fragments arranged by size: 1756 934 747 432 268 161 AflIII A'CryG_T Cuts at: 1650 2754 3731 1650 Size: 1104 977 2217 Fragments arranged by size: 2217 1104 977 AgeI A'CCGG_T Cuts at: 2686 2686 Size: 4298 AlwNI CAG_nnn'CTG Cuts at: 1603 3170 1603 Size: 1567 2731 ApaLI G'TGCA_C Cuts at: 3068 3068 Size: 4298 ApoI r'AATT_y Cuts at: 339 669 909 1302 3651 3907 339 Size: 330 240 393 2349 256 730 Fragments arranged by size: 2349 730 393 330 256 240 AseI AT'TA_AT Cuts at: 20 20 Size: 4298 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4298 AvaI C'yCGr_G Cuts at: 1318 1318 Size: 4298 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 620 653 620 Size: 33 4265 BamHI G'GATC_C Cuts at: 1323 1323 Size: 4298 BanI G'GyrC_C Cuts at: 315 1314 1728 1976 2011 315 Size: 999 414 248 35 2602 Fragments arranged by size: 2602 999 414 248 35 BanII G_rGCy'C Cuts at: 1312 2342 1312 Size: 1030 3268 BbeI G_GCGC'C Cuts at: 1732 1980 1732 Size: 248 4050 BbsI GAAGACnn'nnnn_ Cuts at: 560 693 560 Size: 133 4165 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 4264 BciVI GTATCCnnnnn_n' Cuts at: 2196 2957 4298 2196 Size: 761 1341 2196 Fragments arranged by size: 2196 1341 761 BclI T'GATC_A Cuts at: 1818 1818 Size: 4298 BglI GCCn_nnn'nGGC Cuts at: 422 422 Size: 4298 BglII A'GATC_T Cuts at: 1813 3850 1813 Size: 2037 2261 BlpI GC'TnA_GC Cuts at: 1034 1410 1034 Size: 376 3922 Bme1580I G_kGCm'C Cuts at: 1923 2016 3072 1923 Size: 93 1056 3149 Fragments arranged by size: 3149 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1510 1591 1926 4070 1510 Size: 81 335 2144 1738 Fragments arranged by size: 2144 1738 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1170 2665 1170 Size: 1495 2803 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1474 2353 2845 3143 1474 Size: 879 492 298 2629 Fragments arranged by size: 2629 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4002 4002 Size: 4298 BsaAI yAC'GTr Cuts at: 1651 1651 Size: 4298 BsaBI GATnn'nnATC Cuts at: 6 1812 3849 6 Size: 1806 2037 455 Fragments arranged by size: 2037 1806 455 BsaHI Gr'CG_yC Cuts at: 1729 1977 2679 4175 1729 Size: 248 702 1496 1852 Fragments arranged by size: 1852 1496 702 248 BsaWI w'CCGG_w Cuts at: 1487 2008 2686 2960 3107 3518 1487 Size: 521 678 274 147 411 2267 Fragments arranged by size: 2267 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 201 231 201 Size: 30 4268 BseYI C'CCAG_C Cuts at: 939 1723 2481 3058 3554 939 Size: 784 758 577 496 1683 Fragments arranged by size: 1683 784 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 263 263 Size: 4298 BsiEI CG_ry'CG Cuts at: 104 1840 1886 3094 104 Size: 1736 46 1208 1308 Fragments arranged by size: 1736 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1312 2090 2280 3072 1312 Size: 778 190 792 2538 Fragments arranged by size: 2538 792 778 190 Bsp1286I G_dGCh'C Cuts at: 1312 1923 2016 2090 2280 2342 3072 1312 Size: 611 93 74 190 62 730 2538 Fragments arranged by size: 2538 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3518 3518 Size: 4298 BspHI T'CATG_A Cuts at: 3474 3474 Size: 4298 BspMI ACCTGCnnnn'nnnn_ Cuts at: 381 1268 1348 1562 1864 2245 381 Size: 887 80 214 302 381 2434 Fragments arranged by size: 2434 887 381 302 214 80 BsrBI CCG'CTC Cuts at: 50 2590 2644 4128 4293 50 Size: 2540 54 1484 165 55 Fragments arranged by size: 2540 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2210 2210 Size: 4298 BsrFI r'CCGG_y Cuts at: 2296 2477 2686 2296 Size: 181 209 3908 Fragments arranged by size: 3908 209 181 BssSI C'ACGA_G Cuts at: 992 2569 2927 992 Size: 1577 358 2363 Fragments arranged by size: 2363 1577 358 BstAPI GCAn_nnn'nTGC Cuts at: 1675 1675 Size: 4298 BstBI TT'CG_AA Cuts at: 1250 2659 1250 Size: 1409 2889 BstXI CCAn_nnnn'nTGG Cuts at: 933 933 Size: 4298 BstYI r'GATC_y Cuts at: 1 1323 1790 1813 2148 2394 3406 3492 3504 Size: 1322 467 23 335 246 1012 86 12 Cuts at: 3504 3850 1 Size: 346 449 Fragments arranged by size: 1322 1012 467 449 346 335 246 86 23 12 BstZ17I GTA'TAC Cuts at: 1215 1215 Size: 4298 BtgI C'CryG_G Cuts at: 184 250 184 Size: 66 4232 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2248 2429 2592 2248 Size: 181 163 3954 Fragments arranged by size: 3954 181 163 BtsI GCAGTG_nn' Cuts at: 1541 1541 Size: 4298 DraI TTT'AAA Cuts at: 1297 1297 Size: 4298 DrdI GACnn_nn'nnGTC Cuts at: 764 2004 2862 764 Size: 1240 858 2200 Fragments arranged by size: 2200 1240 858 EaeI y'GGCC_r Cuts at: 1883 2057 2448 2475 3820 1883 Size: 174 391 27 1345 2361 Fragments arranged by size: 2361 1345 391 174 27 EagI C'GGCC_G Cuts at: 1883 1883 Size: 4298 EarI CTCTTCn'nnn_ Cuts at: 440 2321 2531 440 Size: 1881 210 2207 Fragments arranged by size: 2207 1881 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2506 2816 2962 4108 2506 Size: 310 146 1146 2696 Fragments arranged by size: 2696 1146 310 146 EcoICRI GAG'CTC Cuts at: 1310 1310 Size: 4298 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 206 367 2123 2555 3302 3570 206 Size: 161 1756 432 747 268 934 Fragments arranged by size: 1756 934 747 432 268 161 EcoNI CCTnn'n_nnAGG Cuts at: 221 1283 221 Size: 1062 3236 EcoO109I rG'GnC_Cy Cuts at: 1437 1437 Size: 4298 EcoRI G'AATT_C Cuts at: 339 1302 339 Size: 963 3335 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2317 2317 Size: 4298 FspI TGC'GCA Cuts at: 2079 2079 Size: 4298 HaeII r_GCGC'y Cuts at: 1732 1980 3002 3981 1732 Size: 248 1022 979 2049 Fragments arranged by size: 2049 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1165 1197 2140 2170 2172 2202 1165 Size: 32 943 30 2 30 3261 Fragments arranged by size: 3261 943 32 30 30 2 HincII GTy'rAC Cuts at: 740 1184 1337 740 Size: 444 153 3701 Fragments arranged by size: 3701 444 153 HindIII A'AGCT_T Cuts at: 714 714 Size: 4298 HpaI GTT'AAC Cuts at: 740 740 Size: 4298 KasI G'GCGC_C Cuts at: 1728 1976 1728 Size: 248 4050 KpnI G_GTAC'C Cuts at: 1318 1318 Size: 4298 MluI A'CGCG_T Cuts at: 3731 3731 Size: 4298 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1057 2969 3153 1057 Size: 1912 184 2202 Fragments arranged by size: 2202 1912 184 MscI TGG'CCA Cuts at: 2059 2059 Size: 4298 MslI CAynn'nnrTG Cuts at: 2287 2414 2287 Size: 127 4171 MspA1I CmG'CkG Cuts at: 158 469 1409 1723 2083 3096 3341 158 Size: 311 940 314 360 1013 245 1115 Fragments arranged by size: 1115 1013 940 360 314 311 245 NaeI GCC'GGC Cuts at: 2479 2479 Size: 4298 NarI GG'CG_CC Cuts at: 1729 1977 1729 Size: 248 4050 NcoI C'CATG_G Cuts at: 250 250 Size: 4298 NgoMIV G'CCGG_C Cuts at: 2477 2477 Size: 4298 NspI r_CATG'y Cuts at: 738 1351 1851 2758 738 Size: 613 500 907 2278 Fragments arranged by size: 2278 907 613 500 PciI A'CATG_T Cuts at: 2754 2754 Size: 4298 PmeI GTTT'AAAC Cuts at: 1297 1297 Size: 4298 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 230 262 3437 3469 230 Size: 32 3175 32 1059 Fragments arranged by size: 3175 1059 32 32 PstI C_TGCA'G Cuts at: 202 1345 2030 202 Size: 1143 685 2470 Fragments arranged by size: 2470 1143 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4298 PvuII CAG'CTG Cuts at: 158 1723 2083 158 Size: 1565 360 2373 Fragments arranged by size: 2373 1565 360 RsrII CG'GwC_CG Cuts at: 2493 2493 Size: 4298 SacI G_AGCT'C Cuts at: 1312 1312 Size: 4298 SalI G'TCGA_C Cuts at: 1335 1335 Size: 4298 SapI GCTCTTCn'nnn_ Cuts at: 2321 2531 2321 Size: 210 4088 SbfI CC_TGCA'GG Cuts at: 1345 1345 Size: 4298 ScaI AGT'ACT Cuts at: 3815 3815 Size: 4298 SfcI C'TryA_G Cuts at: 33 198 1341 2026 3019 3210 33 Size: 165 1143 685 993 191 1121 Fragments arranged by size: 1143 1121 993 685 191 165 SfoI GGC'GCC Cuts at: 1730 1978 1730 Size: 248 4050 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4298 SmaI CCC'GGG Cuts at: 1320 1320 Size: 4298 SmlI C'TyrA_G Cuts at: 1453 2368 2860 3122 1453 Size: 915 492 262 2629 Fragments arranged by size: 2629 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4298 SphI G_CATG'C Cuts at: 1351 1851 1351 Size: 500 3798 SspI AAT'ATT Cuts at: 510 510 Size: 4298 StyI C'CwwG_G Cuts at: 250 1432 1786 250 Size: 1182 354 2762 Fragments arranged by size: 2762 1182 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 599 1881 2014 2473 2682 599 Size: 1282 133 459 209 2215 Fragments arranged by size: 2215 1282 459 209 133 TatI w'GTAC_w Cuts at: 535 3813 535 Size: 3278 1020 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3815 3847 3815 Size: 32 4266 Tth111I GACn'n_nGTC Cuts at: 2095 2095 Size: 4298 XbaI T'CTAG_A Cuts at: 816 1329 816 Size: 513 3785 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1793 1793 Size: 4298 XmaI C'CCGG_G Cuts at: 1318 1318 Size: 4298 XmnI GAAnn'nnTTC Cuts at: 824 824 Size: 4298 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BbvCI BfrBI BmgBI BmtI BpmI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI