Restriction map for
pF1KB5387
(Circular) (Six-base) MAPSORT of: pF1KB5387.seq Check: 4 from: 1 to: 4742
pF1KB5387 4742 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 1780 1780
Size: 4742
Acc65I G'GTAC_C
Cuts at: 1758 1758
Size: 4742
AclI AA'CG_TT
Cuts at: 4582 4582
Size: 4742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2999 3352 4014 2567
Size: 432 353 662 3295
Fragments arranged by size:
3295 662 432 353
AfeI AGC'GCT
Cuts at: 1627 1627
Size: 4742
AflIII A'CryG_T
Cuts at: 2094 3894 4175 2094
Size: 1800 281 2661
Fragments arranged by size:
2661 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1724 1724
Size: 4742
AlwNI CAG_nnn'CTG
Cuts at: 750 2047 3485 750
Size: 1297 1438 2007
Fragments arranged by size:
2007 1438 1297
ApaI G_GGCC'C
Cuts at: 879 879
Size: 4742
ApaLI G'TGCA_C
Cuts at: 1198 3580 1198
Size: 2382 2360
ApoI r'AATT_y
Cuts at: 271 1746 4095 4351 271
Size: 1475 2349 256 662
Fragments arranged by size:
2349 1475 662 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4742
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
AvaI C'yCGr_G
Cuts at: 677 1762 677
Size: 1085 3657
BamHI G'GATC_C
Cuts at: 723 1767 723
Size: 1044 3698
BanI G'GyrC_C
Cuts at: 309 771 1296 1758 2172 2420 2455 309
Size: 462 525 462 414 248 35 2596
Fragments arranged by size:
2596 525 462 462 414 248 35
BanII G_rGCy'C
Cuts at: 259 536 805 879 1691 1756 2786 259
Size: 277 269 74 812 65 1030 2215
Fragments arranged by size:
2215 1030 812 277 269 74 65
BbeI G_GCGC'C
Cuts at: 775 2176 2424 775
Size: 1401 248 3093
Fragments arranged by size:
3093 1401 248
BbvCI CC'TCA_GC
Cuts at: 550 932 550
Size: 382 4360
BciVI GTATCCnnnnn_n'
Cuts at: 2640 3696 4742 2640
Size: 1056 1046 2640
Fragments arranged by size:
2640 1056 1046
BclI T'GATC_A
Cuts at: 188 303 2262 188
Size: 115 1959 2668
Fragments arranged by size:
2668 1959 115
BglII A'GATC_T
Cuts at: 2257 4294 2257
Size: 2037 2705
BlpI GC'TnA_GC
Cuts at: 1854 1854
Size: 4742
Bme1580I G_kGCm'C
Cuts at: 199 879 1202 2367 2460 3584 199
Size: 680 323 1165 93 1124 1357
Fragments arranged by size:
1357 1165 1124 680 323 93
BmgBI CAC'GTC
Cuts at: 390 1432 390
Size: 1042 3700
BmrI ACTGGGnnnn_n'
Cuts at: 437 1221 1650 1954 2035 2370 4514 437
Size: 784 429 304 81 335 2144 665
Fragments arranged by size:
2144 784 665 429 335 304 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3109 3109
Size: 4742
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 401 548 1102 243
Size: 158 147 554 3883
Fragments arranged by size:
3883 554 158 147
Bpu10I CC'TnA_GC
Cuts at: 225 550 932 225
Size: 325 382 4035
Fragments arranged by size:
4035 382 325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1918 2797 3511 3809 1918
Size: 879 714 298 2851
Fragments arranged by size:
2851 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 142 377 1539 1590 4446 142
Size: 235 1162 51 2856 438
Fragments arranged by size:
2856 1162 438 235 51
BsaAI yAC'GTr
Cuts at: 1335 2095 1335
Size: 760 3982
BsaBI GATnn'nnATC
Cuts at: 6 2256 4293 6
Size: 2250 2037 455
Fragments arranged by size:
2250 2037 455
BsaHI Gr'CG_yC
Cuts at: 772 2173 2421 3123 4619 772
Size: 1401 248 702 1496 895
Fragments arranged by size:
1496 1401 895 702 248
BsaWI w'CCGG_w
Cuts at: 1604 1931 2452 3130 3541 3688 3962 1604
Size: 327 521 678 411 147 274 2384
Fragments arranged by size:
2384 678 521 411 327 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 374 404 629 659 374
Size: 30 225 30 4457
Fragments arranged by size:
4457 225 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 559 580 665 795 1120 1699 559
Size: 21 85 130 325 579 3602
Fragments arranged by size:
3602 579 325 130 85 21
BseYI C'CCAG_C
Cuts at: 374 1181 2167 2925 3590 3998 374
Size: 807 986 758 665 408 1118
Fragments arranged by size:
1118 986 807 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1091 1091
Size: 4742
BsiEI CG_ry'CG
Cuts at: 104 942 1158 2284 2330 3560 104
Size: 838 216 1126 46 1230 1286
Fragments arranged by size:
1286 1230 1126 838 216 46
BsiHKAI G_wGCw'C
Cuts at: 536 1202 1691 1756 2534 2724 3584 536
Size: 666 489 65 778 190 860 1694
Fragments arranged by size:
1694 860 778 666 489 190 65
BsmI GAATG_Cn'
Cuts at: 998 998
Size: 4742
Bsp1286I G_dGCh'C
Cuts at: 199 259 536 805 879 1202 1691 1756 2367
Size: 60 277 269 74 323 489 65 611
Cuts at: 2367 2460 2534 2724 2786 3584 199
Size: 93 74 190 62 798 1357
Fragments arranged by size:
1357 798 611 489 323 277 269 190
93 74 74 65 62 60
BspHI T'CATG_A
Cuts at: 1253 3174 1253
Size: 1921 2821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1110 1692 1792 2006 2308 2689 1110
Size: 582 100 214 302 381 3163
Fragments arranged by size:
3163 582 381 302 214 100
BsrBI CCG'CTC
Cuts at: 50 224 3034 3088 4572 4737 50
Size: 174 2810 54 1484 165 55
Fragments arranged by size:
2810 1484 174 165 55 54
BsrDI GCAATG_nn'
Cuts at: 983 2654 983
Size: 1671 3071
BsrFI r'CCGG_y
Cuts at: 994 1148 1211 2740 2921 994
Size: 154 63 1529 181 2815
Fragments arranged by size:
2815 1529 181 154 63
BssSI C'ACGA_G
Cuts at: 955 1637 3013 3721 955
Size: 682 1376 708 1976
Fragments arranged by size:
1976 1376 708 682
BstAPI GCAn_nnn'nTGC
Cuts at: 2119 2119
Size: 4742
BstBI TT'CG_AA
Cuts at: 3103 3103
Size: 4742
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1425 114
Size: 1311 3431
BstYI r'GATC_y
Cuts at: 1 723 1767 2234 2257 2592 2838 3144 3156
Size: 722 1044 467 23 335 246 306 12
Cuts at: 3156 3242 4294 1
Size: 86 1052 449
Fragments arranged by size:
1052 1044 722 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 807 807
Size: 4742
BtgI C'CryG_G
Cuts at: 107 1292 107
Size: 1185 3557
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2692 2873 3036 2692
Size: 181 163 4398
Fragments arranged by size:
4398 181 163
BtsI GCAGTG_nn'
Cuts at: 637 1108 1418 1985 637
Size: 471 310 567 3394
Fragments arranged by size:
3394 567 471 310
DraI TTT'AAA
Cuts at: 1741 1741
Size: 4742
DrdI GACnn_nn'nnGTC
Cuts at: 2448 3792 2448
Size: 1344 3398
EaeI y'GGCC_r
Cuts at: 116 734 2327 2501 2892 2919 4264 116
Size: 618 1593 174 391 27 1345 594
Fragments arranged by size:
1593 1345 618 594 391 174 27
EagI C'GGCC_G
Cuts at: 2327 2327
Size: 4742
EarI CTCTTCn'nnn_
Cuts at: 1514 2765 2975 1514
Size: 1251 210 3281
Fragments arranged by size:
3281 1251 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2950 3692 3838 4552 2950
Size: 742 146 714 3140
Fragments arranged by size:
3140 742 714 146
EcoICRI GAG'CTC
Cuts at: 534 1689 1754 534
Size: 1155 65 3522
Fragments arranged by size:
3522 1155 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 401 548 1102 2567 2999 3352 4014 243
Size: 158 147 554 1465 432 353 662 971
Fragments arranged by size:
1465 971 662 554 432 353 158 147
EcoO109I rG'GnC_Cy
Cuts at: 875 876 1881 875
Size: 1 1005 3736
Fragments arranged by size:
3736 1005 1
EcoRI G'AATT_C
Cuts at: 1746 1746
Size: 4742
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4742
FspI TGC'GCA
Cuts at: 424 2523 424
Size: 2099 2643
HaeII r_GCGC'y
Cuts at: 775 1062 1629 2176 2424 3654 4425 775
Size: 287 567 547 248 1230 771 1092
Fragments arranged by size:
1230 1092 771 567 547 287 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 153 185 374 406 502 534 708 740 2584
Size: 32 189 32 96 32 174 32 1844
Cuts at: 2584 2614 2616 2646 153
Size: 30 2 30 2249
Fragments arranged by size:
2249 1844 189 174 96 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1781 1781
Size: 4742
KasI G'GCGC_C
Cuts at: 771 2172 2420 771
Size: 1401 248 3093
Fragments arranged by size:
3093 1401 248
KpnI G_GTAC'C
Cuts at: 1762 1762
Size: 4742
MluI A'CGCG_T
Cuts at: 4175 4175
Size: 4742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 459 3501 3685 459
Size: 3042 184 1516
Fragments arranged by size:
3042 1516 184
MscI TGG'CCA
Cuts at: 118 736 2503 118
Size: 618 1767 2357
Fragments arranged by size:
2357 1767 618
MslI CAynn'nnrTG
Cuts at: 112 178 623 1270 2731 2858 112
Size: 66 445 647 1461 127 1996
Fragments arranged by size:
1996 1461 647 445 127 66
MspA1I CmG'CkG
Cuts at: 829 1022 1853 2167 2527 3311 3556 829
Size: 193 831 314 360 784 245 2015
Fragments arranged by size:
2015 831 784 360 314 245 193
NaeI GCC'GGC
Cuts at: 996 1213 2923 996
Size: 217 1710 2815
Fragments arranged by size:
2815 1710 217
NarI GG'CG_CC
Cuts at: 772 2173 2421 772
Size: 1401 248 3093
Fragments arranged by size:
3093 1401 248
NcoI C'CATG_G
Cuts at: 107 1292 107
Size: 1185 3557
NgoMIV G'CCGG_C
Cuts at: 994 1211 2921 994
Size: 217 1710 2815
Fragments arranged by size:
2815 1710 217
NspI r_CATG'y
Cuts at: 1795 2295 3898 1795
Size: 500 1603 2639
Fragments arranged by size:
2639 1603 500
PciI A'CATG_T
Cuts at: 3894 3894
Size: 4742
PfoI T'CCnGG_A
Cuts at: 275 1714 275
Size: 1439 3303
PmeI GTTT'AAAC
Cuts at: 1741 1741
Size: 4742
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4710
PspOMI G'GGCC_C
Cuts at: 875 875
Size: 4742
PspXI vC'TCGA_Gb
Cuts at: 677 677
Size: 4742
PstI C_TGCA'G
Cuts at: 248 1789 2474 248
Size: 1541 685 2516
Fragments arranged by size:
2516 1541 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4742
PvuII CAG'CTG
Cuts at: 829 2167 2527 829
Size: 1338 360 3044
Fragments arranged by size:
3044 1338 360
RsrII CG'GwC_CG
Cuts at: 2937 2937
Size: 4742
SacI G_AGCT'C
Cuts at: 536 1691 1756 536
Size: 1155 65 3522
Fragments arranged by size:
3522 1155 65
SalI G'TCGA_C
Cuts at: 1779 1779
Size: 4742
SapI GCTCTTCn'nnn_
Cuts at: 2765 2975 2765
Size: 210 4532
SbfI CC_TGCA'GG
Cuts at: 1789 1789
Size: 4742
ScaI AGT'ACT
Cuts at: 4259 4259
Size: 4742
SfcI C'TryA_G
Cuts at: 33 244 1785 2470 3438 3629 33
Size: 211 1541 685 968 191 1146
Fragments arranged by size:
1541 1146 968 685 211 191
SfoI GGC'GCC
Cuts at: 773 2174 2422 773
Size: 1401 248 3093
Fragments arranged by size:
3093 1401 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
SmaI CCC'GGG
Cuts at: 1764 1764
Size: 4742
SmlI C'TyrA_G
Cuts at: 677 1897 2812 3526 3788 677
Size: 1220 915 714 262 1631
Fragments arranged by size:
1631 1220 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4742
SphI G_CATG'C
Cuts at: 1795 2295 1795
Size: 500 4242
StyI C'CwwG_G
Cuts at: 107 989 1292 1876 2230 107
Size: 882 303 584 354 2619
Fragments arranged by size:
2619 882 584 354 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1172 2325 2458 2917 3126 1172
Size: 1153 133 459 209 2788
Fragments arranged by size:
2788 1153 459 209 133
TatI w'GTAC_w
Cuts at: 785 4257 785
Size: 3472 1270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 629 661 4259 4291 629
Size: 32 3598 32 1080
Fragments arranged by size:
3598 1080 32 32
Tth111I GACn'n_nGTC
Cuts at: 388 2539 388
Size: 2151 2591
XbaI T'CTAG_A
Cuts at: 1773 1773
Size: 4742
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1485 2237 1485
Size: 752 3990
XhoI C'TCGA_G
Cuts at: 677 677
Size: 4742
XmaI C'CCGG_G
Cuts at: 1762 1762
Size: 4742
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmtI BsiWI
BsmBI BspEI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI