Restriction map for
pF1KB5390
(Circular) (Six-base) MAPSORT of: pF1KB5390.seq Check: 1856 from: 1 to: 5873
pF1KB5390 5873 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AatII G_ACGT'C
Cuts at: 1979 1979
Size: 5873
AccI GT'mk_AC
Cuts at: 1754 2911 1754
Size: 1157 4716
Acc65I G'GTAC_C
Cuts at: 2889 2889
Size: 5873
AclI AA'CG_TT
Cuts at: 5713 5713
Size: 5873
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1599 2201 3698 4130 4483 5145 1599
Size: 602 1497 432 353 662 2327
Fragments arranged by size:
2327 1497 662 602 432 353
AflIII A'CryG_T
Cuts at: 860 926 1116 3225 5025 5306 860
Size: 66 190 2109 1800 281 1427
Fragments arranged by size:
2109 1800 1427 281 190 66
AleI CACnn'nnGTG
Cuts at: 1375 2347 2509 1375
Size: 972 162 4739
Fragments arranged by size:
4739 972 162
AlwNI CAG_nnn'CTG
Cuts at: 136 460 1188 3178 4616 136
Size: 324 728 1990 1438 1393
Fragments arranged by size:
1990 1438 1393 728 324
ApaI G_GGCC'C
Cuts at: 1988 2036 1988
Size: 48 5825
ApaLI G'TGCA_C
Cuts at: 4711 4711
Size: 5873
ApoI r'AATT_y
Cuts at: 2877 5226 5482 2877
Size: 2349 256 3268
Fragments arranged by size:
3268 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5873
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5873
AvaI C'yCGr_G
Cuts at: 662 1844 2489 2893 662
Size: 1182 645 404 3642
Fragments arranged by size:
3642 1182 645 404
BamHI G'GATC_C
Cuts at: 1488 2898 1488
Size: 1410 4463
BanI G'GyrC_C
Cuts at: 224 407 2057 2809 2889 3303 3551 3586 224
Size: 183 1650 752 80 414 248 35 2511
Fragments arranged by size:
2511 1650 752 414 248 183 80 35
BanII G_rGCy'C
Cuts at: 686 1016 1729 1988 2036 2339 2887 3917 686
Size: 330 713 259 48 303 548 1030 2642
Fragments arranged by size:
2642 1030 713 548 330 303 259 48
BbeI G_GCGC'C
Cuts at: 228 411 2813 3307 3555 228
Size: 183 2402 494 248 2546
Fragments arranged by size:
2546 2402 494 248 183
BbsI GAAGACnn'nnnn_
Cuts at: 698 1464 1605 698
Size: 766 141 4966
Fragments arranged by size:
4966 766 141
BbvCI CC'TCA_GC
Cuts at: 958 2261 958
Size: 1303 4570
BciVI GTATCCnnnnn_n'
Cuts at: 3771 4827 5873 3771
Size: 1056 1046 3771
Fragments arranged by size:
3771 1056 1046
BclI T'GATC_A
Cuts at: 791 3393 791
Size: 2602 3271
BglI GCCn_nnn'nGGC
Cuts at: 2106 2106
Size: 5873
BglII A'GATC_T
Cuts at: 1541 3388 5425 1541
Size: 1847 2037 1989
Fragments arranged by size:
2037 1989 1847
BlpI GC'TnA_GC
Cuts at: 2985 2985
Size: 5873
Bme1580I G_kGCm'C
Cuts at: 1384 1844 1988 2036 2062 3498 3591 4715 1384
Size: 460 144 48 26 1436 93 1124 2542
Fragments arranged by size:
2542 1436 1124 460 144 93 48 26
BmgBI CAC'GTC
Cuts at: 2772 2772
Size: 5873
BmrI ACTGGGnnnn_n'
Cuts at: 559 791 1042 2003 2226 3085 3166 3501 5645
Size: 232 251 961 223 859 81 335 2144
Cuts at: 5645 559
Size: 787
Fragments arranged by size:
2144 961 859 787 335 251 232 223
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4240 4240
Size: 5873
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 1027 2063 2380 333
Size: 694 1036 317 3826
Fragments arranged by size:
3826 1036 694 317
Bpu10I CC'TnA_GC
Cuts at: 958 2015 2261 958
Size: 1057 246 4570
Fragments arranged by size:
4570 1057 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 796 1713 3049 3928 4642 4940 397
Size: 399 917 1336 879 714 298 1330
Fragments arranged by size:
1336 1330 917 879 714 399 298
BsaI GGTCTCn'nnnn_
Cuts at: 796 2003 2347 5577 796
Size: 1207 344 3230 1092
Fragments arranged by size:
3230 1207 1092 344
BsaAI yAC'GTr
Cuts at: 327 929 1905 2271 2295 3226 327
Size: 602 976 366 24 931 2974
Fragments arranged by size:
2974 976 931 602 366 24
BsaBI GATnn'nnATC
Cuts at: 6 2449 3387 5424 6
Size: 2443 938 2037 455
Fragments arranged by size:
2443 2037 938 455
BsaHI Gr'CG_yC
Cuts at: 225 408 1976 2781 2810 3304 3552 4254 5750
Size: 183 1568 805 29 494 248 702 1496
Cuts at: 5750 225
Size: 348
Fragments arranged by size:
1568 1496 805 702 494 348 248 183
29
BsaWI w'CCGG_w
Cuts at: 1278 2577 3062 3583 4261 4672 4819 5093 1278
Size: 1299 485 521 678 411 147 274 2058
Fragments arranged by size:
2058 1299 678 521 485 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 331 361 1087 1117 1177 1207 1777 1807 1811
Size: 30 726 30 60 30 570 30 4
Cuts at: 1811 1841 1855 1885 2689 2719 331
Size: 30 14 30 804 30 3485
Fragments arranged by size:
3485 804 726 570 60 30 30 30
30 30 30 30 14 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 1024 1663 1975 211
Size: 813 639 312 4109
Fragments arranged by size:
4109 813 639 312
BseYI C'CCAG_C
Cuts at: 453 829 1270 2029 2035 2195 2814 2842 3298
Size: 376 441 759 6 160 619 28 456
Cuts at: 3298 4056 4721 5129 453
Size: 758 665 408 1197
Fragments arranged by size:
1197 759 758 665 619 456 441 408
376 160 28 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 349
Size: 5873
BsiEI CG_ry'CG
Cuts at: 104 119 1279 2166 2798 3415 3461 4691 104
Size: 15 1160 887 632 617 46 1230 1286
Fragments arranged by size:
1286 1230 1160 887 632 617 46 15
BsiHKAI G_wGCw'C
Cuts at: 1016 2339 2887 3665 3855 4715 1016
Size: 1323 548 778 190 860 2174
Fragments arranged by size:
2174 1323 860 778 548 190
BsmBI CGTCTCn'nnnn_
Cuts at: 2709 2709
Size: 5873
Bsp1286I G_dGCh'C
Cuts at: 686 1016 1384 1729 1844 1988 2036 2062 2339
Size: 330 368 345 115 144 48 26 277
Cuts at: 2339 2887 3498 3591 3665 3855 3917 4715 686
Size: 548 611 93 74 190 62 798 1844
Fragments arranged by size:
1844 798 611 548 368 345 330 277
190 144 115 93 74 62 48 26
BspEI T'CCGG_A
Cuts at: 2577 2577
Size: 5873
BspHI T'CATG_A
Cuts at: 848 935 4305 848
Size: 87 3370 2416
Fragments arranged by size:
3370 2416 87
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1766 2923 3137 3439 3820 1766
Size: 1157 214 302 381 3819
Fragments arranged by size:
3819 1157 381 302 214
BsrBI CCG'CTC
Cuts at: 50 669 1103 4165 4219 5703 5868 50
Size: 619 434 3062 54 1484 165 55
Fragments arranged by size:
3062 1484 619 434 165 55 54
BsrDI GCAATG_nn'
Cuts at: 969 1401 2204 3785 969
Size: 432 803 1581 3057
Fragments arranged by size:
3057 1581 803 432
BsrFI r'CCGG_y
Cuts at: 271 1514 1889 2797 3871 4052 271
Size: 1243 375 908 1074 181 2092
Fragments arranged by size:
2092 1243 1074 908 375 181
BsrGI T'GTAC_A
Cuts at: 2585 2585
Size: 5873
BssSI C'ACGA_G
Cuts at: 193 1519 2509 4144 4852 193
Size: 1326 990 1635 708 1214
Fragments arranged by size:
1635 1326 1214 990 708
BstAPI GCAn_nnn'nTGC
Cuts at: 929 3250 929
Size: 2321 3552
BstBI TT'CG_AA
Cuts at: 4234 4234
Size: 5873
BstXI CCAn_nnnn'nTGG
Cuts at: 2421 2421
Size: 5873
BstYI r'GATC_y
Cuts at: 1 1488 1541 1548 2898 3365 3388 3723 3969
Size: 1487 53 7 1350 467 23 335 246
Cuts at: 3969 4275 4287 4373 5425 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1487 1350 1052 467 449 335 306 246
86 53 23 12 7
BtgI C'CryG_G
Cuts at: 107 1353 1484 107
Size: 1246 131 4496
Fragments arranged by size:
4496 1246 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1277 2586 3823 4004 4167 1277
Size: 1309 1237 181 163 2983
Fragments arranged by size:
2983 1309 1237 181 163
BtsI GCAGTG_nn'
Cuts at: 262 319 958 1438 3116 262
Size: 57 639 480 1678 3019
Fragments arranged by size:
3019 1678 639 480 57
DraI TTT'AAA
Cuts at: 2872 2872
Size: 5873
DrdI GACnn_nn'nnGTC
Cuts at: 2701 3579 4923 2701
Size: 878 1344 3651
Fragments arranged by size:
3651 1344 878
EaeI y'GGCC_r
Cuts at: 501 1092 1137 2673 2795 3458 3632 4023 4050
Size: 591 45 1536 122 663 174 391 27
Cuts at: 4050 5395 501
Size: 1345 979
Fragments arranged by size:
1536 1345 979 663 591 391 174 122
45 27
EagI C'GGCC_G
Cuts at: 2795 3458 2795
Size: 663 5210
EarI CTCTTCn'nnn_
Cuts at: 863 1366 2344 2758 3896 4106 863
Size: 503 978 414 1138 210 2630
Fragments arranged by size:
2630 1138 978 503 414 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1338 4081 4823 4969 5683 1338
Size: 2743 742 146 714 1528
Fragments arranged by size:
2743 1528 742 714 146
EcoICRI GAG'CTC
Cuts at: 1014 2337 2885 1014
Size: 1323 548 4002
Fragments arranged by size:
4002 1323 548
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 1027 1599 2063 2201 2380 3698 4130 4483
Size: 694 572 464 138 179 1318 432 353
Cuts at: 4483 5145 333
Size: 662 1061
Fragments arranged by size:
1318 1061 694 662 572 464 432 353
179 138
EcoNI CCTnn'n_nnAGG
Cuts at: 415 2362 415
Size: 1947 3926
EcoO109I rG'GnC_Cy
Cuts at: 1221 1866 1985 2529 3012 1221
Size: 645 119 544 483 4082
Fragments arranged by size:
4082 645 544 483 119
EcoRI G'AATT_C
Cuts at: 2877 2877
Size: 5873
EcoRV GAT'ATC
Cuts at: 1683 1683
Size: 5873
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1479 3892 1479
Size: 2413 3460
FspI TGC'GCA
Cuts at: 655 3654 655
Size: 2999 2874
HaeII r_GCGC'y
Cuts at: 228 411 2813 3307 3555 4785 5556 228
Size: 183 2402 494 248 1230 771 545
Fragments arranged by size:
2402 1230 771 545 494 248 183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 331 363 1177 1209 1533 1565 1724 1756 1811
Size: 32 814 32 324 32 159 32 55
Cuts at: 1811 1843 3715 3745 3747 3777 331
Size: 32 1872 30 2 30 2427
Fragments arranged by size:
2427 1872 814 324 159 55 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2912 2912
Size: 5873
KasI G'GCGC_C
Cuts at: 224 407 2809 3303 3551 224
Size: 183 2402 494 248 2546
Fragments arranged by size:
2546 2402 494 248 183
KpnI G_GTAC'C
Cuts at: 2893 2893
Size: 5873
MfeI C'AATT_G
Cuts at: 2076 2076
Size: 5873
MluI A'CGCG_T
Cuts at: 5306 5306
Size: 5873
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 845 2636 4632 4816 845
Size: 1791 1996 184 1902
Fragments arranged by size:
1996 1902 1791 184
MscI TGG'CCA
Cuts at: 503 1094 1139 3634 503
Size: 591 45 2495 2742
Fragments arranged by size:
2742 2495 591 45
MslI CAynn'nnrTG
Cuts at: 1375 2094 2347 2509 3862 3989 1375
Size: 719 253 162 1353 127 3259
Fragments arranged by size:
3259 1353 719 253 162 127
MspA1I CmG'CkG
Cuts at: 133 163 460 482 840 2124 2406 2672 2984
Size: 30 297 22 358 1284 282 266 312
Cuts at: 2984 3298 3658 4442 4687 133
Size: 314 360 784 245 1319
Fragments arranged by size:
1319 1284 784 360 358 314 312 297
282 266 245 30 22
NaeI GCC'GGC
Cuts at: 273 2799 4054 273
Size: 2526 1255 2092
Fragments arranged by size:
2526 2092 1255
NarI GG'CG_CC
Cuts at: 225 408 2810 3304 3552 225
Size: 183 2402 494 248 2546
Fragments arranged by size:
2546 2402 494 248 183
NcoI C'CATG_G
Cuts at: 107 1484 107
Size: 1377 4496
NgoMIV G'CCGG_C
Cuts at: 271 2797 4052 271
Size: 2526 1255 2092
Fragments arranged by size:
2526 2092 1255
NspI r_CATG'y
Cuts at: 847 864 1120 2631 2926 3426 5029 847
Size: 17 256 1511 295 500 1603 1691
Fragments arranged by size:
1691 1603 1511 500 295 256 17
PasI CC'CwG_GG
Cuts at: 1981 1981
Size: 5873
PciI A'CATG_T
Cuts at: 860 1116 5025 860
Size: 256 3909 1708
Fragments arranged by size:
3909 1708 256
PflMI CCAn_nnn'nTGG
Cuts at: 1158 2772 1158
Size: 1614 4259
PfoI T'CCnGG_A
Cuts at: 1202 2069 2525 1202
Size: 867 456 4550
Fragments arranged by size:
4550 867 456
PmeI GTTT'AAAC
Cuts at: 2872 2872
Size: 5873
PmlI CAC'GTG
Cuts at: 929 929
Size: 5873
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4319 4351 4319
Size: 32 5841
PpuMI rG'GwC_Cy
Cuts at: 1221 2529 1221
Size: 1308 4565
PspOMI G'GGCC_C
Cuts at: 1984 2032 1984
Size: 48 5825
PstI C_TGCA'G
Cuts at: 953 2138 2324 2833 2920 3605 953
Size: 1185 186 509 87 685 3221
Fragments arranged by size:
3221 1185 685 509 186 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5873
PvuII CAG'CTG
Cuts at: 133 460 482 840 2124 2406 3298 3658 133
Size: 327 22 358 1284 282 892 360 2348
Fragments arranged by size:
2348 1284 892 360 358 327 282 22
RsrII CG'GwC_CG
Cuts at: 4068 4068
Size: 5873
SacI G_AGCT'C
Cuts at: 1016 2339 2887 1016
Size: 1323 548 4002
Fragments arranged by size:
4002 1323 548
SalI G'TCGA_C
Cuts at: 2910 2910
Size: 5873
SapI GCTCTTCn'nnn_
Cuts at: 2344 3896 4106 2344
Size: 1552 210 4111
Fragments arranged by size:
4111 1552 210
SbfI CC_TGCA'GG
Cuts at: 2920 2920
Size: 5873
ScaI AGT'ACT
Cuts at: 985 5390 985
Size: 4405 1468
SexAI A'CCwGG_T
Cuts at: 473 1070 473
Size: 597 5276
SfcI C'TryA_G
Cuts at: 33 949 2134 2320 2829 2916 3601 4569 4760
Size: 916 1185 186 509 87 685 968 191
Cuts at: 4760 33
Size: 1146
Fragments arranged by size:
1185 1146 968 916 685 509 191 186
87
SfoI GGC'GCC
Cuts at: 226 409 2811 3305 3553 226
Size: 183 2402 494 248 2546
Fragments arranged by size:
2546 2402 494 248 183
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5873
SmaI CCC'GGG
Cuts at: 1846 2895 1846
Size: 1049 4824
SmlI C'TyrA_G
Cuts at: 412 775 1728 3028 3943 4657 4919 412
Size: 363 953 1300 915 714 262 1366
Fragments arranged by size:
1366 1300 953 915 714 363 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5873
SphI G_CATG'C
Cuts at: 2631 2926 3426 2631
Size: 295 500 5078
Fragments arranged by size:
5078 500 295
StuI AGG'CCT
Cuts at: 209 1060 209
Size: 851 5022
StyI C'CwwG_G
Cuts at: 107 1484 2082 2104 2735 3007 3361 107
Size: 1377 598 22 631 272 354 2619
Fragments arranged by size:
2619 1377 631 598 354 272 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 525 2856 3456 3589 4048 4257 525
Size: 2331 600 133 459 209 2141
Fragments arranged by size:
2331 2141 600 459 209 133
TatI w'GTAC_w
Cuts at: 983 2585 5388 983
Size: 1602 2803 1468
Fragments arranged by size:
2803 1602 1468
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 205 237 1087 1119 5390 5422 205
Size: 32 850 32 4271 32 656
Fragments arranged by size:
4271 850 656 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2284 3670 2284
Size: 1386 4487
XbaI T'CTAG_A
Cuts at: 492 2904 492
Size: 2412 3461
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 461 3368 461
Size: 2907 2966
XmaI C'CCGG_G
Cuts at: 1844 2893 1844
Size: 1049 4824
ZraI GAC'GTC
Cuts at: 1977 1977
Size: 5873
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmtI BsiWI BsmI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI