Restriction map for
pF1KB5394
(Circular) (Six-base) MAPSORT of: pF1KB5394.seq Check: 40 from: 1 to: 4892
pF1KB5394 4892 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AccI GT'mk_AC
Cuts at: 1930 1930
Size: 4892
Acc65I G'GTAC_C
Cuts at: 1908 1908
Size: 4892
AclI AA'CG_TT
Cuts at: 4732 4732
Size: 4892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1793 2717 3149 3896 4164 1793
Size: 924 432 747 268 2521
Fragments arranged by size:
2521 924 747 432 268
AfeI AGC'GCT
Cuts at: 1416 1416
Size: 4892
AflIII A'CryG_T
Cuts at: 488 827 1199 2244 3348 4325 488
Size: 339 372 1045 1104 977 1055
Fragments arranged by size:
1104 1055 1045 977 372 339
AgeI A'CCGG_T
Cuts at: 3280 3280
Size: 4892
AhdI GACnn_n'nnGTC
Cuts at: 516 516
Size: 4892
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 304 304
Size: 4892
AlwNI CAG_nnn'CTG
Cuts at: 204 459 474 1609 2197 3764 204
Size: 255 15 1135 588 1567 1332
Fragments arranged by size:
1567 1332 1135 588 255 15
ApaLI G'TGCA_C
Cuts at: 3662 3662
Size: 4892
ApoI r'AATT_y
Cuts at: 255 1896 4245 4501 255
Size: 1641 2349 256 646
Fragments arranged by size:
2349 1641 646 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4892
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
AvaI C'yCGr_G
Cuts at: 1030 1078 1248 1861 1912 1030
Size: 48 170 613 51 4010
Fragments arranged by size:
4010 613 170 51 48
BamHI G'GATC_C
Cuts at: 1917 1917
Size: 4892
BanI G'GyrC_C
Cuts at: 280 569 662 1326 1426 1618 1908 2322 2570
Size: 289 93 664 100 192 290 414 248
Cuts at: 2570 2605 280
Size: 35 2567
Fragments arranged by size:
2567 664 414 290 289 248 192 100
93 35
BanII G_rGCy'C
Cuts at: 383 734 997 1318 1373 1412 1530 1906 2936
Size: 351 263 321 55 39 118 376 1030
Cuts at: 2936 383
Size: 2339
Fragments arranged by size:
2339 1030 376 351 321 263 118 55
39
BbeI G_GCGC'C
Cuts at: 2326 2574 2326
Size: 248 4644
BbsI GAAGACnn'nnnn_
Cuts at: 1848 1848
Size: 4892
BbvCI CC'TCA_GC
Cuts at: 1183 1183
Size: 4892
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1472 1506 1472
Size: 34 4858
BciVI GTATCCnnnnn_n'
Cuts at: 2790 3551 4892 2790
Size: 761 1341 2790
Fragments arranged by size:
2790 1341 761
BclI T'GATC_A
Cuts at: 2412 2412
Size: 4892
BglI GCCn_nnn'nGGC
Cuts at: 387 1069 387
Size: 682 4210
BglII A'GATC_T
Cuts at: 2407 4444 2407
Size: 2037 2855
BlpI GC'TnA_GC
Cuts at: 2004 2004
Size: 4892
Bme1580I G_kGCm'C
Cuts at: 283 567 1429 2517 2610 3666 283
Size: 284 862 1088 93 1056 1509
Fragments arranged by size:
1509 1088 1056 862 284 93
BmrI ACTGGGnnnn_n'
Cuts at: 651 956 1230 1879 2104 2185 2520 4664 651
Size: 305 274 649 225 81 335 2144 879
Fragments arranged by size:
2144 879 649 335 305 274 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3259 3259
Size: 4892
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 871 937 989 1544 1551 1858 181
Size: 690 66 52 555 7 307 3215
Fragments arranged by size:
3215 690 555 307 66 52 7
Bpu10I CC'TnA_GC
Cuts at: 1183 1183
Size: 4892
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 1714 2068 2947 3439 3737 661
Size: 1053 354 879 492 298 1816
Fragments arranged by size:
1816 1053 879 492 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 214 1588 4596 214
Size: 1374 3008 510
Fragments arranged by size:
3008 1374 510
BsaAI yAC'GTr
Cuts at: 491 2245 491
Size: 1754 3138
BsaBI GATnn'nnATC
Cuts at: 6 2406 4443 6
Size: 2400 2037 455
Fragments arranged by size:
2400 2037 455
BsaHI Gr'CG_yC
Cuts at: 1853 2323 2571 3273 4769 1853
Size: 470 248 702 1496 1976
Fragments arranged by size:
1976 1496 702 470 248
BsaWI w'CCGG_w
Cuts at: 302 1112 2081 2602 3280 3554 3701 4112 302
Size: 810 969 521 678 274 147 411 1082
Fragments arranged by size:
1082 969 810 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 301 331 775 805 301
Size: 30 444 30 4388
Fragments arranged by size:
4388 444 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 739 742 772 796 739
Size: 3 30 24 4835
Fragments arranged by size:
4835 30 24 3
BseYI C'CCAG_C
Cuts at: 204 956 1411 1447 1602 1688 2317 3075 3652
Size: 752 455 36 155 86 629 758 577
Cuts at: 3652 4148 204
Size: 496 948
Fragments arranged by size:
948 758 752 629 577 496 455 155
86 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 181
Size: 4892
BsiEI CG_ry'CG
Cuts at: 104 1064 1460 2434 2480 3688 104
Size: 960 396 974 46 1208 1308
Fragments arranged by size:
1308 1208 974 960 396 46
BsiHKAI G_wGCw'C
Cuts at: 734 1906 2684 2874 3666 734
Size: 1172 778 190 792 1960
Fragments arranged by size:
1960 1172 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1537 1537
Size: 4892
Bsp1286I G_dGCh'C
Cuts at: 283 383 567 734 997 1318 1373 1412 1429
Size: 100 184 167 263 321 55 39 17
Cuts at: 1429 1530 1906 2517 2610 2684 2874 2936 3666
Size: 101 376 611 93 74 190 62 730
Cuts at: 3666 283
Size: 1509
Fragments arranged by size:
1509 730 611 376 321 263 190 184
167 101 100 93 74 62 55 39
17
BspEI T'CCGG_A
Cuts at: 302 1112 4112 302
Size: 810 3000 1082
Fragments arranged by size:
3000 1082 810
BspHI T'CATG_A
Cuts at: 4068 4068
Size: 4892
BspMI ACCTGCnnnn'nnnn_
Cuts at: 440 713 1524 1664 1942 2156 2458 2839 440
Size: 273 811 140 278 214 302 381 2493
Fragments arranged by size:
2493 811 381 302 278 273 214 140
BsrBI CCG'CTC
Cuts at: 50 3184 3238 4722 4887 50
Size: 3134 54 1484 165 55
Fragments arranged by size:
3134 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 524 2804 524
Size: 2280 2612
BsrFI r'CCGG_y
Cuts at: 1059 1063 1387 2890 3071 3280 1059
Size: 4 324 1503 181 209 2671
Fragments arranged by size:
2671 1503 324 209 181 4
BssHII G'CGCG_C
Cuts at: 179 179
Size: 4892
BssSI C'ACGA_G
Cuts at: 334 1048 3163 3521 334
Size: 714 2115 358 1705
Fragments arranged by size:
2115 1705 714 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2269 2269
Size: 4892
BstBI TT'CG_AA
Cuts at: 3253 3253
Size: 4892
BstXI CCAn_nnnn'nTGG
Cuts at: 1278 1278
Size: 4892
BstYI r'GATC_y
Cuts at: 1 297 771 1178 1917 2384 2407 2742 2988
Size: 296 474 407 739 467 23 335 246
Cuts at: 2988 4000 4086 4098 4444 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 739 474 467 449 407 346 335
296 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 482 1354 1622 1848 107
Size: 375 872 268 226 3151
Fragments arranged by size:
3151 872 375 268 226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 825 2842 3023 3186 825
Size: 2017 181 163 2531
Fragments arranged by size:
2531 2017 181 163
BtsI GCAGTG_nn'
Cuts at: 250 1641 2135 250
Size: 1391 494 3007
Fragments arranged by size:
3007 1391 494
DraI TTT'AAA
Cuts at: 1891 1891
Size: 4892
DrdI GACnn_nn'nnGTC
Cuts at: 1204 2598 3456 1204
Size: 1394 858 2640
Fragments arranged by size:
2640 1394 858
EaeI y'GGCC_r
Cuts at: 1061 1457 1703 2477 2651 3042 3069 4414 1061
Size: 396 246 774 174 391 27 1345 1539
Fragments arranged by size:
1539 1345 774 396 391 246 174 27
EagI C'GGCC_G
Cuts at: 1061 1457 2477 1061
Size: 396 1020 3476
Fragments arranged by size:
3476 1020 396
EarI CTCTTCn'nnn_
Cuts at: 1580 1769 2915 3125 1580
Size: 189 1146 210 3347
Fragments arranged by size:
3347 1146 210 189
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1124 3100 3410 3556 4702 1124
Size: 1976 310 146 1146 1314
Fragments arranged by size:
1976 1314 1146 310 146
EcoICRI GAG'CTC
Cuts at: 732 1904 732
Size: 1172 3720
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 871 937 989 1544 1551 1793 1858 2717
Size: 690 66 52 555 7 242 65 859
Cuts at: 2717 3149 3896 4164 181
Size: 432 747 268 909
Fragments arranged by size:
909 859 747 690 555 432 268 242
66 65 52 7
EcoNI CCTnn'n_nnAGG
Cuts at: 980 1192 980
Size: 212 4680
EcoO109I rG'GnC_Cy
Cuts at: 1245 1252 1359 1468 1508 1881 2031 1245
Size: 7 107 109 40 373 150 4106
Fragments arranged by size:
4106 373 150 109 107 40 7
EcoRI G'AATT_C
Cuts at: 1896 1896
Size: 4892
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2911 2911
Size: 4892
FspI TGC'GCA
Cuts at: 2673 2673
Size: 4892
HaeII r_GCGC'y
Cuts at: 475 1418 1650 2326 2574 3596 4575 475
Size: 943 232 676 248 1022 979 792
Fragments arranged by size:
1022 979 943 792 676 248 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 502 534 775 807 2734 2764 2766 2796 502
Size: 32 241 32 1927 30 2 30 2598
Fragments arranged by size:
2598 1927 241 32 32 30 30 2
HincII GTy'rAC
Cuts at: 825 1044 1931 825
Size: 219 887 3786
Fragments arranged by size:
3786 887 219
KasI G'GCGC_C
Cuts at: 2322 2570 2322
Size: 248 4644
KpnI G_GTAC'C
Cuts at: 1912 1912
Size: 4892
MluI A'CGCG_T
Cuts at: 4325 4325
Size: 4892
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3563 3747 3563
Size: 184 4708
MscI TGG'CCA
Cuts at: 2653 2653
Size: 4892
MslI CAynn'nnrTG
Cuts at: 1801 2881 3008 1801
Size: 1080 127 3685
Fragments arranged by size:
3685 1080 127
MspA1I CmG'CkG
Cuts at: 270 2003 2317 2677 3690 3935 270
Size: 1733 314 360 1013 245 1227
Fragments arranged by size:
1733 1227 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1061 1065 1389 3073 1061
Size: 4 324 1684 2880
Fragments arranged by size:
2880 1684 324 4
NarI GG'CG_CC
Cuts at: 2323 2571 2323
Size: 248 4644
NcoI C'CATG_G
Cuts at: 107 482 1354 107
Size: 375 872 3645
Fragments arranged by size:
3645 872 375
NgoMIV G'CCGG_C
Cuts at: 1059 1063 1387 3071 1059
Size: 4 324 1684 2880
Fragments arranged by size:
2880 1684 324 4
NspI r_CATG'y
Cuts at: 1945 2445 3352 1945
Size: 500 907 3485
Fragments arranged by size:
3485 907 500
PciI A'CATG_T
Cuts at: 3348 3348
Size: 4892
PflMI CCAn_nnn'nTGG
Cuts at: 204 459 204
Size: 255 4637
PmeI GTTT'AAAC
Cuts at: 1891 1891
Size: 4892
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 4031
Size: 32 4860
PpuMI rG'GwC_Cy
Cuts at: 1359 1508 1881 1359
Size: 149 373 4370
Fragments arranged by size:
4370 373 149
PstI C_TGCA'G
Cuts at: 293 368 680 779 1727 1939 2624 293
Size: 75 312 99 948 212 685 2561
Fragments arranged by size:
2561 948 685 312 212 99 75
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4892
PvuII CAG'CTG
Cuts at: 270 2317 2677 270
Size: 2047 360 2485
Fragments arranged by size:
2485 2047 360
RsrII CG'GwC_CG
Cuts at: 3087 3087
Size: 4892
SacI G_AGCT'C
Cuts at: 734 1906 734
Size: 1172 3720
SalI G'TCGA_C
Cuts at: 1929 1929
Size: 4892
SanDI GG'GwC_CC
Cuts at: 1508 1881 1508
Size: 373 4519
SapI GCTCTTCn'nnn_
Cuts at: 1769 2915 3125 1769
Size: 1146 210 3536
Fragments arranged by size:
3536 1146 210
SbfI CC_TGCA'GG
Cuts at: 1939 1939
Size: 4892
ScaI AGT'ACT
Cuts at: 4409 4409
Size: 4892
SfcI C'TryA_G
Cuts at: 33 289 364 676 775 1723 1935 2620 3613
Size: 256 75 312 99 948 212 685 993
Cuts at: 3613 3804 33
Size: 191 1121
Fragments arranged by size:
1121 993 948 685 312 256 212 191
99 75
SfiI GGCCn_nnn'nGGCC
Cuts at: 1069 1069
Size: 4892
SfoI GGC'GCC
Cuts at: 2324 2572 2324
Size: 248 4644
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
SmaI CCC'GGG
Cuts at: 1080 1250 1914 1080
Size: 170 664 4058
Fragments arranged by size:
4058 664 170
SmlI C'TyrA_G
Cuts at: 640 1729 2047 2962 3454 3716 640
Size: 1089 318 915 492 262 1816
Fragments arranged by size:
1816 1089 915 492 318 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4892
SphI G_CATG'C
Cuts at: 1945 2445 1945
Size: 500 4392
StuI AGG'CCT
Cuts at: 1489 1489
Size: 4892
StyI C'CwwG_G
Cuts at: 107 224 482 666 1157 1354 2026 2380 107
Size: 117 258 184 491 197 672 354 2619
Fragments arranged by size:
2619 672 491 354 258 197 184 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1344 2475 2608 3067 3276 1344
Size: 1131 133 459 209 2960
Fragments arranged by size:
2960 1131 459 209 133
TatI w'GTAC_w
Cuts at: 4407 4407
Size: 4892
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 325 357 565 597 1138 1170 1365 1397 4409
Size: 32 208 32 541 32 195 32 3012
Cuts at: 4409 4441 325
Size: 32 776
Fragments arranged by size:
3012 776 541 208 195 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2689 2689
Size: 4892
XbaI T'CTAG_A
Cuts at: 1923 1923
Size: 4892
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1361 1519 2387 114
Size: 1247 158 868 2619
Fragments arranged by size:
2619 1247 868 158
XmaI C'CCGG_G
Cuts at: 1078 1248 1912 1078
Size: 170 664 4058
Fragments arranged by size:
4058 664 170
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AloI ApaI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI