Restriction map for
pF1KB5396
(Circular) (Six-base) MAPSORT of: pF1KB5396.seq Check: 9049 from: 1 to: 5012
pF1KB5396 5012 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 2050 2050
Size: 5012
Acc65I G'GTAC_C
Cuts at: 2028 2028
Size: 5012
AclI AA'CG_TT
Cuts at: 4852 4852
Size: 5012
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1903 2837 3269 3622 4284 1903
Size: 934 432 353 662 2631
Fragments arranged by size:
2631 934 662 432 353
AflIII A'CryG_T
Cuts at: 375 2364 4164 4445 375
Size: 1989 1800 281 942
Fragments arranged by size:
1989 1800 942 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1876 1908 1876
Size: 32 4980
AlwNI CAG_nnn'CTG
Cuts at: 2317 3755 2317
Size: 1438 3574
ApaLI G'TGCA_C
Cuts at: 3850 3850
Size: 5012
ApoI r'AATT_y
Cuts at: 316 797 901 1195 1972 2016 4365 4621 316
Size: 481 104 294 777 44 2349 256 707
Fragments arranged by size:
2349 777 707 481 294 256 104 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 5012
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5012
AvaI C'yCGr_G
Cuts at: 1790 2032 1790
Size: 242 4770
BamHI G'GATC_C
Cuts at: 702 859 2037 702
Size: 157 1178 3677
Fragments arranged by size:
3677 1178 157
BanI G'GyrC_C
Cuts at: 651 1707 2028 2442 2690 2725 651
Size: 1056 321 414 248 35 2938
Fragments arranged by size:
2938 1056 414 321 248 35
BanII G_rGCy'C
Cuts at: 336 2026 3056 336
Size: 1690 1030 2292
Fragments arranged by size:
2292 1690 1030
BbeI G_GCGC'C
Cuts at: 2446 2694 2446
Size: 248 4764
BbsI GAAGACnn'nnnn_
Cuts at: 421 1259 421
Size: 838 4174
BciVI GTATCCnnnnn_n'
Cuts at: 159 1977 2910 3966 5012 159
Size: 1818 933 1056 1046 159
Fragments arranged by size:
1818 1056 1046 933 159
BclI T'GATC_A
Cuts at: 2532 2532
Size: 5012
BfrBI ATG'CAT
Cuts at: 733 1237 733
Size: 504 4508
BglII A'GATC_T
Cuts at: 2527 4564 2527
Size: 2037 2975
BlpI GC'TnA_GC
Cuts at: 1907 2124 1907
Size: 217 4795
Bme1580I G_kGCm'C
Cuts at: 656 1710 2637 2730 3854 656
Size: 1054 927 93 1124 1814
Fragments arranged by size:
1814 1124 1054 927 93
BmrI ACTGGGnnnn_n'
Cuts at: 1896 2224 2305 2640 4784 1896
Size: 328 81 335 2144 2124
Fragments arranged by size:
2144 2124 335 328 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3379 3379
Size: 5012
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1368 1475 1368
Size: 107 4905
Bpu10I CC'TnA_GC
Cuts at: 1121 1121
Size: 5012
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 2188 3067 3781 4079 520
Size: 1668 879 714 298 1453
Fragments arranged by size:
1668 1453 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4716 4716
Size: 5012
BsaAI yAC'GTr
Cuts at: 2365 2365
Size: 5012
BsaBI GATnn'nnATC
Cuts at: 6 2526 4563 6
Size: 2520 2037 455
Fragments arranged by size:
2520 2037 455
BsaHI Gr'CG_yC
Cuts at: 1026 2443 2691 3393 4889 1026
Size: 1417 248 702 1496 1149
Fragments arranged by size:
1496 1417 1149 702 248
BsaWI w'CCGG_w
Cuts at: 712 2201 2722 3400 3811 3958 4232 712
Size: 1489 521 678 411 147 274 1492
Fragments arranged by size:
1492 1489 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1253 1283 1253
Size: 30 4982
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1274 1412 1415 1628 1631 1274
Size: 138 3 213 3 4655
Fragments arranged by size:
4655 213 138 3 3
BseYI C'CCAG_C
Cuts at: 2437 3195 3860 4268 2437
Size: 758 665 408 3181
Fragments arranged by size:
3181 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 746 746
Size: 5012
BsiEI CG_ry'CG
Cuts at: 104 2554 2600 3830 104
Size: 2450 46 1230 1286
Fragments arranged by size:
2450 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 336 1641 1668 2026 2804 2994 3854 336
Size: 1305 27 358 778 190 860 1494
Fragments arranged by size:
1494 1305 860 778 358 190 27
Bsp1286I G_dGCh'C
Cuts at: 336 656 1641 1668 1710 2026 2637 2730 2804
Size: 320 985 27 42 316 611 93 74
Cuts at: 2804 2994 3056 3854 336
Size: 190 62 798 1494
Fragments arranged by size:
1494 985 798 611 320 316 190 93
74 62 42 27
BspHI T'CATG_A
Cuts at: 3444 3444
Size: 5012
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2062 2276 2578 2959 2062
Size: 214 302 381 4115
Fragments arranged by size:
4115 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3304 3358 4842 5007 50
Size: 3254 54 1484 165 55
Fragments arranged by size:
3254 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1815 2924 1815
Size: 1109 3903
BsrFI r'CCGG_y
Cuts at: 1028 3010 3191 1028
Size: 1982 181 2849
Fragments arranged by size:
2849 1982 181
BssSI C'ACGA_G
Cuts at: 3283 3991 3283
Size: 708 4304
BstAPI GCAn_nnn'nTGC
Cuts at: 304 2389 304
Size: 2085 2927
BstBI TT'CG_AA
Cuts at: 3373 3373
Size: 5012
BstYI r'GATC_y
Cuts at: 1 684 702 859 1104 1569 1786 2037 2504
Size: 683 18 157 245 465 217 251 467
Cuts at: 2504 2527 2862 3108 3414 3426 3512 4564 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 683 467 465 449 335 306 251
246 245 217 157 86 23 18 12
BtgI C'CryG_G
Cuts at: 107 1773 1782 107
Size: 1666 9 3337
Fragments arranged by size:
3337 1666 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1887 2962 3143 3306 1887
Size: 1075 181 163 3593
Fragments arranged by size:
3593 1075 181 163
BtsI GCAGTG_nn'
Cuts at: 1391 2255 1391
Size: 864 4148
DraI TTT'AAA
Cuts at: 2011 2011
Size: 5012
DrdI GACnn_nn'nnGTC
Cuts at: 2718 4062 2718
Size: 1344 3668
EaeI y'GGCC_r
Cuts at: 2597 2771 3162 3189 4534 2597
Size: 174 391 27 1345 3075
Fragments arranged by size:
3075 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2597 2597
Size: 5012
EarI CTCTTCn'nnn_
Cuts at: 143 1605 3035 3245 143
Size: 1462 1430 210 1910
Fragments arranged by size:
1910 1462 1430 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3220 3962 4108 4822 3220
Size: 742 146 714 3410
Fragments arranged by size:
3410 742 714 146
EcoICRI GAG'CTC
Cuts at: 334 2024 334
Size: 1690 3322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1368 1475 1903 2837 3269 3622 4284 1368
Size: 107 428 934 432 353 662 2096
Fragments arranged by size:
2096 934 662 432 428 353 107
EcoNI CCTnn'n_nnAGG
Cuts at: 1676 1676
Size: 5012
EcoO109I rG'GnC_Cy
Cuts at: 469 1750 2151 469
Size: 1281 401 3330
Fragments arranged by size:
3330 1281 401
EcoRI G'AATT_C
Cuts at: 901 2016 901
Size: 1115 3897
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 5012
FspI TGC'GCA
Cuts at: 2793 2793
Size: 5012
HaeII r_GCGC'y
Cuts at: 1365 2446 2694 3924 4695 1365
Size: 1081 248 1230 771 1682
Fragments arranged by size:
1682 1230 1081 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 694 726 991 1023 2854 2884 2886 2916 694
Size: 32 265 32 1831 30 2 30 2790
Fragments arranged by size:
2790 1831 265 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2051 2051
Size: 5012
KasI G'GCGC_C
Cuts at: 2442 2690 2442
Size: 248 4764
KpnI G_GTAC'C
Cuts at: 2032 2032
Size: 5012
MfeI C'AATT_G
Cuts at: 299 299
Size: 5012
MluI A'CGCG_T
Cuts at: 4445 4445
Size: 5012
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 837 3771 3955 837
Size: 2934 184 1894
Fragments arranged by size:
2934 1894 184
MscI TGG'CCA
Cuts at: 2773 2773
Size: 5012
MslI CAynn'nnrTG
Cuts at: 399 832 3001 3128 399
Size: 433 2169 127 2283
Fragments arranged by size:
2283 2169 433 127
MspA1I CmG'CkG
Cuts at: 1267 2123 2437 2797 3581 3826 1267
Size: 856 314 360 784 245 2453
Fragments arranged by size:
2453 856 784 360 314 245
NaeI GCC'GGC
Cuts at: 1030 3193 1030
Size: 2163 2849
NarI GG'CG_CC
Cuts at: 2443 2691 2443
Size: 248 4764
NcoI C'CATG_G
Cuts at: 107 107
Size: 5012
NgoMIV G'CCGG_C
Cuts at: 1028 3191 1028
Size: 2163 2849
NsiI A_TGCA'T
Cuts at: 735 1239 735
Size: 504 4508
NspI r_CATG'y
Cuts at: 379 1434 2065 2565 4168 379
Size: 1055 631 500 1603 1223
Fragments arranged by size:
1603 1223 1055 631 500
PciI A'CATG_T
Cuts at: 375 4164 375
Size: 3789 1223
PmeI GTTT'AAAC
Cuts at: 2011 2011
Size: 5012
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3458 3490 3458
Size: 32 4980
PpuMI rG'GwC_Cy
Cuts at: 469 1750 469
Size: 1281 3731
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 498 530 498
Size: 32 4980
PstI C_TGCA'G
Cuts at: 2059 2744 2059
Size: 685 4327
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5012
PvuII CAG'CTG
Cuts at: 1267 2437 2797 1267
Size: 1170 360 3482
Fragments arranged by size:
3482 1170 360
RsrII CG'GwC_CG
Cuts at: 3207 3207
Size: 5012
SacI G_AGCT'C
Cuts at: 336 2026 336
Size: 1690 3322
SalI G'TCGA_C
Cuts at: 2049 2049
Size: 5012
SapI GCTCTTCn'nnn_
Cuts at: 3035 3245 3035
Size: 210 4802
SbfI CC_TGCA'GG
Cuts at: 2059 2059
Size: 5012
ScaI AGT'ACT
Cuts at: 4529 4529
Size: 5012
SfcI C'TryA_G
Cuts at: 33 346 420 2055 2740 3708 3899 33
Size: 313 74 1635 685 968 191 1146
Fragments arranged by size:
1635 1146 968 685 313 191 74
SfoI GGC'GCC
Cuts at: 2444 2692 2444
Size: 248 4764
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5012
SmaI CCC'GGG
Cuts at: 2034 2034
Size: 5012
SmlI C'TyrA_G
Cuts at: 499 2167 3082 3796 4058 499
Size: 1668 915 714 262 1453
Fragments arranged by size:
1668 1453 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5012
SphI G_CATG'C
Cuts at: 1434 2065 2565 1434
Size: 631 500 3881
Fragments arranged by size:
3881 631 500
SspI AAT'ATT
Cuts at: 254 1175 254
Size: 921 4091
StuI AGG'CCT
Cuts at: 218 493 1381 218
Size: 275 888 3849
Fragments arranged by size:
3849 888 275
StyI C'CwwG_G
Cuts at: 107 625 816 1651 2146 2500 107
Size: 518 191 835 495 354 2619
Fragments arranged by size:
2619 835 518 495 354 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2595 2728 3187 3396 2595
Size: 133 459 209 4211
Fragments arranged by size:
4211 459 209 133
TatI w'GTAC_w
Cuts at: 342 572 4527 342
Size: 230 3955 827
Fragments arranged by size:
3955 827 230
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 534 566 4529 4561 534
Size: 32 3963 32 985
Fragments arranged by size:
3963 985 32 32
Tth111I GACn'n_nGTC
Cuts at: 2809 2809
Size: 5012
XbaI T'CTAG_A
Cuts at: 2043 2043
Size: 5012
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2507 2507
Size: 5012
XmaI C'CCGG_G
Cuts at: 2032 2032
Size: 5012
XmnI GAAnn'nnTTC
Cuts at: 254 254
Size: 5012
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PmeI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI