Restriction map for
pF1KB5399
(Circular) (Six-base) MAPSORT of: pF1KB5399.seq Check: 2948 from: 1 to: 3686
pF1KB5399 3686 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 560 724 560
Size: 164 3522
Acc65I G'GTAC_C
Cuts at: 702 702
Size: 3686
AclI AA'CG_TT
Cuts at: 3526 3526
Size: 3686
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 1511 1943 2690 2958 158
Size: 1353 432 747 268 886
Fragments arranged by size:
1353 886 747 432 268
AflIII A'CryG_T
Cuts at: 1038 2142 3119 1038
Size: 1104 977 1605
Fragments arranged by size:
1605 1104 977
AgeI A'CCGG_T
Cuts at: 2074 2074
Size: 3686
AleI CACnn'nnGTG
Cuts at: 460 460
Size: 3686
AlwNI CAG_nnn'CTG
Cuts at: 408 991 2558 408
Size: 583 1567 1536
Fragments arranged by size:
1567 1536 583
ApaLI G'TGCA_C
Cuts at: 2456 2456
Size: 3686
ApoI r'AATT_y
Cuts at: 247 616 690 3039 3295 247
Size: 369 74 2349 256 638
Fragments arranged by size:
2349 638 369 256 74
AseI AT'TA_AT
Cuts at: 20 20
Size: 3686
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3686
AvaI C'yCGr_G
Cuts at: 241 458 706 241
Size: 217 248 3221
Fragments arranged by size:
3221 248 217
BamHI G'GATC_C
Cuts at: 711 711
Size: 3686
BanI G'GyrC_C
Cuts at: 340 702 1116 1364 1399 340
Size: 362 414 248 35 2627
Fragments arranged by size:
2627 414 362 248 35
BanII G_rGCy'C
Cuts at: 700 1730 700
Size: 1030 2656
BbeI G_GCGC'C
Cuts at: 1120 1368 1120
Size: 248 3438
BciVI GTATCCnnnnn_n'
Cuts at: 1584 2345 3686 1584
Size: 761 1341 1584
Fragments arranged by size:
1584 1341 761
BclI T'GATC_A
Cuts at: 512 1206 512
Size: 694 2992
BglII A'GATC_T
Cuts at: 1201 3238 1201
Size: 2037 1649
BlpI GC'TnA_GC
Cuts at: 798 798
Size: 3686
Bme1580I G_kGCm'C
Cuts at: 1311 1404 2460 1311
Size: 93 1056 2537
Fragments arranged by size:
2537 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 423 898 979 1314 3458 423
Size: 475 81 335 2144 651
Fragments arranged by size:
2144 651 475 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 3686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 369 493 369
Size: 124 3562
Bpu10I CC'TnA_GC
Cuts at: 548 548
Size: 3686
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 196 862 1741 2233 2531 172
Size: 24 666 879 492 298 1327
Fragments arranged by size:
1327 879 666 492 298 24
BsaI GGTCTCn'nnnn_
Cuts at: 3390 3390
Size: 3686
BsaAI yAC'GTr
Cuts at: 1039 1039
Size: 3686
BsaBI GATnn'nnATC
Cuts at: 6 1200 3237 6
Size: 1194 2037 455
Fragments arranged by size:
2037 1194 455
BsaHI Gr'CG_yC
Cuts at: 287 544 1117 1365 2067 3563 287
Size: 257 573 248 702 1496 410
Fragments arranged by size:
1496 702 573 410 257 248
BsaWI w'CCGG_w
Cuts at: 875 1396 2074 2348 2495 2906 875
Size: 521 678 274 147 411 1655
Fragments arranged by size:
1655 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 302 1111 1869 2446 2942 302
Size: 809 758 577 496 1046
Fragments arranged by size:
1046 809 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1228 1274 2482 104
Size: 1124 46 1208 1308
Fragments arranged by size:
1308 1208 1124 46
BsiHKAI G_wGCw'C
Cuts at: 166 700 1478 1668 2460 166
Size: 534 778 190 792 1392
Fragments arranged by size:
1392 792 778 534 190
BsmBI CGTCTCn'nnnn_
Cuts at: 278 278
Size: 3686
Bsp1286I G_dGCh'C
Cuts at: 166 700 1311 1404 1478 1668 1730 2460 166
Size: 534 611 93 74 190 62 730 1392
Fragments arranged by size:
1392 730 611 534 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2906 2906
Size: 3686
BspHI T'CATG_A
Cuts at: 515 2862 515
Size: 2347 1339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 736 950 1252 1633 736
Size: 214 302 381 2789
Fragments arranged by size:
2789 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1978 2032 3516 3681 50
Size: 1928 54 1484 165 55
Fragments arranged by size:
1928 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1598 1598
Size: 3686
BsrFI r'CCGG_y
Cuts at: 1684 1865 2074 1684
Size: 181 209 3296
Fragments arranged by size:
3296 209 181
BssSI C'ACGA_G
Cuts at: 1957 2315 1957
Size: 358 3328
BstAPI GCAn_nnn'nTGC
Cuts at: 1063 1063
Size: 3686
BstBI TT'CG_AA
Cuts at: 2047 2047
Size: 3686
BstYI r'GATC_y
Cuts at: 1 711 1178 1201 1536 1782 2794 2880 2892
Size: 710 467 23 335 246 1012 86 12
Cuts at: 2892 3238 1
Size: 346 449
Fragments arranged by size:
1012 710 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3686
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1636 1817 1980 1636
Size: 181 163 3342
Fragments arranged by size:
3342 181 163
BtsI GCAGTG_nn'
Cuts at: 199 670 929 199
Size: 471 259 2956
Fragments arranged by size:
2956 471 259
DraI TTT'AAA
Cuts at: 685 685
Size: 3686
DrdI GACnn_nn'nnGTC
Cuts at: 1392 2250 1392
Size: 858 2828
EaeI y'GGCC_r
Cuts at: 1271 1445 1836 1863 3208 1271
Size: 174 391 27 1345 1749
Fragments arranged by size:
1749 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1271 1271
Size: 3686
EarI CTCTTCn'nnn_
Cuts at: 154 1709 1919 154
Size: 1555 210 1921
Fragments arranged by size:
1921 1555 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1894 2204 2350 3496 1894
Size: 310 146 1146 2084
Fragments arranged by size:
2084 1146 310 146
EcoICRI GAG'CTC
Cuts at: 698 698
Size: 3686
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 369 493 1511 1943 2690 2958 158
Size: 211 124 1018 432 747 268 886
Fragments arranged by size:
1018 886 747 432 268 211 124
EcoO109I rG'GnC_Cy
Cuts at: 150 825 150
Size: 675 3011
EcoRI G'AATT_C
Cuts at: 616 690 616
Size: 74 3612
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1705 1705
Size: 3686
FspI TGC'GCA
Cuts at: 1467 1467
Size: 3686
HaeII r_GCGC'y
Cuts at: 1120 1368 2390 3369 1120
Size: 248 1022 979 1437
Fragments arranged by size:
1437 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 222 254 347 379 1528 1558 1560 1590 222
Size: 32 93 32 1149 30 2 30 2318
Fragments arranged by size:
2318 1149 93 32 32 30 30 2
HincII GTy'rAC
Cuts at: 725 725
Size: 3686
KasI G'GCGC_C
Cuts at: 1116 1364 1116
Size: 248 3438
KpnI G_GTAC'C
Cuts at: 706 706
Size: 3686
MluI A'CGCG_T
Cuts at: 3119 3119
Size: 3686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2357 2541 2357
Size: 184 3502
MscI TGG'CCA
Cuts at: 1447 1447
Size: 3686
MslI CAynn'nnrTG
Cuts at: 460 1675 1802 460
Size: 1215 127 2344
Fragments arranged by size:
2344 1215 127
MspA1I CmG'CkG
Cuts at: 797 1111 1471 2484 2729 797
Size: 314 360 1013 245 1754
Fragments arranged by size:
1754 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1867 1867
Size: 3686
NarI GG'CG_CC
Cuts at: 1117 1365 1117
Size: 248 3438
NcoI C'CATG_G
Cuts at: 107 107
Size: 3686
NgoMIV G'CCGG_C
Cuts at: 1865 1865
Size: 3686
NspI r_CATG'y
Cuts at: 310 739 1239 2146 310
Size: 429 500 907 1850
Fragments arranged by size:
1850 907 500 429
PciI A'CATG_T
Cuts at: 2142 2142
Size: 3686
PmeI GTTT'AAAC
Cuts at: 685 685
Size: 3686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2857 2825
Size: 32 3654
PpuMI rG'GwC_Cy
Cuts at: 150 150
Size: 3686
PstI C_TGCA'G
Cuts at: 665 733 1418 665
Size: 68 685 2933
Fragments arranged by size:
2933 685 68
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3686
PvuII CAG'CTG
Cuts at: 1111 1471 1111
Size: 360 3326
RsrII CG'GwC_CG
Cuts at: 1881 1881
Size: 3686
SacI G_AGCT'C
Cuts at: 700 700
Size: 3686
SalI G'TCGA_C
Cuts at: 723 723
Size: 3686
SapI GCTCTTCn'nnn_
Cuts at: 154 1709 1919 154
Size: 1555 210 1921
Fragments arranged by size:
1921 1555 210
SbfI CC_TGCA'GG
Cuts at: 733 733
Size: 3686
ScaI AGT'ACT
Cuts at: 3203 3203
Size: 3686
SexAI A'CCwGG_T
Cuts at: 152 152
Size: 3686
SfcI C'TryA_G
Cuts at: 33 661 729 1414 2407 2598 33
Size: 628 68 685 993 191 1121
Fragments arranged by size:
1121 993 685 628 191 68
SfoI GGC'GCC
Cuts at: 1118 1366 1118
Size: 248 3438
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3686
SmaI CCC'GGG
Cuts at: 708 708
Size: 3686
SmlI C'TyrA_G
Cuts at: 187 211 241 841 1756 2248 2510 187
Size: 24 30 600 915 492 262 1363
Fragments arranged by size:
1363 915 600 492 262 30 24
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3686
SphI G_CATG'C
Cuts at: 310 739 1239 310
Size: 429 500 2757
Fragments arranged by size:
2757 500 429
StyI C'CwwG_G
Cuts at: 107 820 1174 107
Size: 713 354 2619
Fragments arranged by size:
2619 713 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1269 1402 1861 2070 1269
Size: 133 459 209 2885
Fragments arranged by size:
2885 459 209 133
TatI w'GTAC_w
Cuts at: 3201 3201
Size: 3686
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 3654
Tth111I GACn'n_nGTC
Cuts at: 1483 1483
Size: 3686
XbaI T'CTAG_A
Cuts at: 717 717
Size: 3686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1181 1181
Size: 3686
XhoI C'TCGA_G
Cuts at: 241 241
Size: 3686
XmaI C'CCGG_G
Cuts at: 706 706
Size: 3686
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BseRI BsgI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI