Restriction map for
pF1KB5400
(Circular) (Six-base) MAPSORT of: pF1KB5400.seq Check: 2157 from: 1 to: 4526
pF1KB5400 4526 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1564 1564
Size: 4526
Acc65I G'GTAC_C
Cuts at: 1333 1542 1333
Size: 209 4317
AclI AA'CG_TT
Cuts at: 4366 4366
Size: 4526
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 2351 2783 3136 3798 304
Size: 2047 432 353 662 1032
Fragments arranged by size:
2047 1032 662 432 353
AflIII A'CryG_T
Cuts at: 1878 3678 3959 1878
Size: 1800 281 2445
Fragments arranged by size:
2445 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1354 1483 1831 3269 1354
Size: 129 348 1438 2611
Fragments arranged by size:
2611 1438 348 129
ApaLI G'TGCA_C
Cuts at: 3364 3364
Size: 4526
ApoI r'AATT_y
Cuts at: 288 1492 1530 3879 4135 288
Size: 1204 38 2349 256 679
Fragments arranged by size:
2349 1204 679 256 38
AseI AT'TA_AT
Cuts at: 20 20
Size: 4526
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4526
AvaI C'yCGr_G
Cuts at: 1546 1546
Size: 4526
BamHI G'GATC_C
Cuts at: 1551 1551
Size: 4526
BanI G'GyrC_C
Cuts at: 1061 1154 1303 1333 1542 1956 2204 2239 1061
Size: 93 149 30 209 414 248 35 3348
Fragments arranged by size:
3348 414 248 209 149 93 35 30
BanII G_rGCy'C
Cuts at: 785 1133 1540 2570 785
Size: 348 407 1030 2741
Fragments arranged by size:
2741 1030 407 348
BbeI G_GCGC'C
Cuts at: 1065 1158 1960 2208 1065
Size: 93 802 248 3383
Fragments arranged by size:
3383 802 248 93
BbsI GAAGACnn'nnnn_
Cuts at: 316 408 316
Size: 92 4434
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1234 1268 90
Size: 34 1110 34 3348
Fragments arranged by size:
3348 1110 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2424 3480 4526 2424
Size: 1056 1046 2424
Fragments arranged by size:
2424 1056 1046
BclI T'GATC_A
Cuts at: 2046 2046
Size: 4526
BglII A'GATC_T
Cuts at: 2041 4078 2041
Size: 2037 2489
BlpI GC'TnA_GC
Cuts at: 1638 1638
Size: 4526
Bme1580I G_kGCm'C
Cuts at: 2151 2244 3368 2151
Size: 93 1124 3309
Fragments arranged by size:
3309 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1738 1819 2154 4298 1738
Size: 81 335 2144 1966
Fragments arranged by size:
2144 1966 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2893 2893
Size: 4526
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 1147 1331 1505 1015
Size: 132 184 174 4036
Fragments arranged by size:
4036 184 174 132
Bpu10I CC'TnA_GC
Cuts at: 1132 1132
Size: 4526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1702 2581 3295 3593 1702
Size: 879 714 298 2635
Fragments arranged by size:
2635 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4230 4230
Size: 4526
BsaAI yAC'GTr
Cuts at: 1879 1879
Size: 4526
BsaBI GATnn'nnATC
Cuts at: 6 2040 4077 6
Size: 2034 2037 455
Fragments arranged by size:
2037 2034 455
BsaHI Gr'CG_yC
Cuts at: 1062 1155 1957 2205 2907 4403 1062
Size: 93 802 248 702 1496 1185
Fragments arranged by size:
1496 1185 802 702 248 93
BsaWI w'CCGG_w
Cuts at: 1715 2236 2914 3325 3472 3746 1715
Size: 521 678 411 147 274 2495
Fragments arranged by size:
2495 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1326 1356 1326
Size: 30 4496
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1334 1448 1334
Size: 114 4412
BseYI C'CCAG_C
Cuts at: 1338 1951 2709 3374 3782 1338
Size: 613 758 665 408 2082
Fragments arranged by size:
2082 758 665 613 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 998 1078 1496 998
Size: 80 418 4028
Fragments arranged by size:
4028 418 80
BsiEI CG_ry'CG
Cuts at: 104 2068 2114 3344 104
Size: 1964 46 1230 1286
Fragments arranged by size:
1964 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1028 1133 1540 2318 2508 3368 1028
Size: 105 407 778 190 860 2186
Fragments arranged by size:
2186 860 778 407 190 105
BsmI GAATG_Cn'
Cuts at: 1465 1465
Size: 4526
Bsp1286I G_dGCh'C
Cuts at: 785 1028 1133 1540 2151 2244 2318 2508 2570
Size: 243 105 407 611 93 74 190 62
Cuts at: 2570 3368 785
Size: 798 1943
Fragments arranged by size:
1943 798 611 407 243 190 105 93
74 62
BspHI T'CATG_A
Cuts at: 2958 2958
Size: 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 240 1362 1576 1790 2092 2473 240
Size: 1122 214 214 302 381 2293
Fragments arranged by size:
2293 1122 381 302 214 214
BsrBI CCG'CTC
Cuts at: 50 2818 2872 4356 4521 50
Size: 2768 54 1484 165 55
Fragments arranged by size:
2768 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2438 2438
Size: 4526
BsrFI r'CCGG_y
Cuts at: 2524 2705 2524
Size: 181 4345
BssSI C'ACGA_G
Cuts at: 983 2797 3505 983
Size: 1814 708 2004
Fragments arranged by size:
2004 1814 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1903 1903
Size: 4526
BstBI TT'CG_AA
Cuts at: 564 2887 564
Size: 2323 2203
BstYI r'GATC_y
Cuts at: 1 1551 2018 2041 2376 2622 2928 2940 3026
Size: 1550 467 23 335 246 306 12 86
Cuts at: 3026 4078 1
Size: 1052 449
Fragments arranged by size:
1550 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 669 669
Size: 4526
BtgI C'CryG_G
Cuts at: 510 785 510
Size: 275 4251
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2476 2657 2820 2476
Size: 181 163 4182
Fragments arranged by size:
4182 181 163
BtsI GCAGTG_nn'
Cuts at: 738 1769 738
Size: 1031 3495
DraI TTT'AAA
Cuts at: 1525 1525
Size: 4526
DraIII CAC_nnn'GTG
Cuts at: 511 511
Size: 4526
DrdI GACnn_nn'nnGTC
Cuts at: 2232 3576 2232
Size: 1344 3182
EaeI y'GGCC_r
Cuts at: 621 2111 2285 2676 2703 4048 621
Size: 1490 174 391 27 1345 1099
Fragments arranged by size:
1490 1345 1099 391 174 27
EagI C'GGCC_G
Cuts at: 2111 2111
Size: 4526
EarI CTCTTCn'nnn_
Cuts at: 800 1082 1139 1160 2549 2759 800
Size: 282 57 21 1389 210 2567
Fragments arranged by size:
2567 1389 282 210 57 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2734 3476 3622 4336 2734
Size: 742 146 714 2924
Fragments arranged by size:
2924 742 714 146
EcoICRI GAG'CTC
Cuts at: 1131 1538 1131
Size: 407 4119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 1015 1147 1331 1505 2351 2783 3136 3798
Size: 711 132 184 174 846 432 353 662
Cuts at: 3798 304
Size: 1032
Fragments arranged by size:
1032 846 711 662 432 353 184 174
132
EcoO109I rG'GnC_Cy
Cuts at: 665 1351 1665 665
Size: 686 314 3526
Fragments arranged by size:
3526 686 314
EcoRI G'AATT_C
Cuts at: 1530 1530
Size: 4526
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 829 1090 1242 2545 829
Size: 261 152 1303 2810
Fragments arranged by size:
2810 1303 261 152
FspI TGC'GCA
Cuts at: 2307 2307
Size: 4526
HaeII r_GCGC'y
Cuts at: 1065 1158 1960 2208 3438 4209 1065
Size: 93 802 248 1230 771 1382
Fragments arranged by size:
1382 1230 802 771 248 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2368 2398 2400 2430 2368
Size: 30 2 30 4464
Fragments arranged by size:
4464 30 30 2
HincII GTy'rAC
Cuts at: 1565 1565
Size: 4526
KasI G'GCGC_C
Cuts at: 1061 1154 1956 2204 1061
Size: 93 802 248 3383
Fragments arranged by size:
3383 802 248 93
KpnI G_GTAC'C
Cuts at: 1337 1546 1337
Size: 209 4317
MfeI C'AATT_G
Cuts at: 1445 1445
Size: 4526
MluI A'CGCG_T
Cuts at: 3959 3959
Size: 4526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 611 3285 3469 611
Size: 2674 184 1668
Fragments arranged by size:
2674 1668 184
MscI TGG'CCA
Cuts at: 623 2287 623
Size: 1664 2862
MslI CAynn'nnrTG
Cuts at: 934 1039 1363 2515 2642 934
Size: 105 324 1152 127 2818
Fragments arranged by size:
2818 1152 324 127 105
MspA1I CmG'CkG
Cuts at: 522 706 1330 1637 1951 2311 3095 3340 522
Size: 184 624 307 314 360 784 245 1708
Fragments arranged by size:
1708 784 624 360 314 307 245 184
NaeI GCC'GGC
Cuts at: 2707 2707
Size: 4526
NarI GG'CG_CC
Cuts at: 1062 1155 1957 2205 1062
Size: 93 802 248 3383
Fragments arranged by size:
3383 802 248 93
NcoI C'CATG_G
Cuts at: 785 785
Size: 4526
NgoMIV G'CCGG_C
Cuts at: 2705 2705
Size: 4526
NspI r_CATG'y
Cuts at: 1579 2079 3682 1579
Size: 500 1603 2423
Fragments arranged by size:
2423 1603 500
PciI A'CATG_T
Cuts at: 3678 3678
Size: 4526
PflMI CCAn_nnn'nTGG
Cuts at: 511 917 511
Size: 406 4120
PfoI T'CCnGG_A
Cuts at: 1347 1482 1347
Size: 135 4391
PmeI GTTT'AAAC
Cuts at: 1525 1525
Size: 4526
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2972 3004 2972
Size: 32 4494
PpuMI rG'GwC_Cy
Cuts at: 1351 1351
Size: 4526
PsiI TTA'TAA
Cuts at: 832 832
Size: 4526
PstI C_TGCA'G
Cuts at: 1573 2258 1573
Size: 685 3841
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4526
PvuII CAG'CTG
Cuts at: 522 706 1330 1951 2311 522
Size: 184 624 621 360 2737
Fragments arranged by size:
2737 624 621 360 184
RsrII CG'GwC_CG
Cuts at: 2721 2721
Size: 4526
SacI G_AGCT'C
Cuts at: 1133 1540 1133
Size: 407 4119
SalI G'TCGA_C
Cuts at: 1563 1563
Size: 4526
SapI GCTCTTCn'nnn_
Cuts at: 1082 1139 2549 2759 1082
Size: 57 1410 210 2849
Fragments arranged by size:
2849 1410 210 57
SbfI CC_TGCA'GG
Cuts at: 1573 1573
Size: 4526
ScaI AGT'ACT
Cuts at: 4043 4043
Size: 4526
SexAI A'CCwGG_T
Cuts at: 1028 1028
Size: 4526
SfcI C'TryA_G
Cuts at: 33 638 1569 2254 3222 3413 33
Size: 605 931 685 968 191 1146
Fragments arranged by size:
1146 968 931 685 605 191
SfoI GGC'GCC
Cuts at: 1063 1156 1958 2206 1063
Size: 93 802 248 3383
Fragments arranged by size:
3383 802 248 93
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4526
SmaI CCC'GGG
Cuts at: 1548 1548
Size: 4526
SmlI C'TyrA_G
Cuts at: 1681 2596 3310 3572 1681
Size: 915 714 262 2635
Fragments arranged by size:
2635 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4526
SphI G_CATG'C
Cuts at: 1579 2079 1579
Size: 500 4026
StyI C'CwwG_G
Cuts at: 785 1660 2014 785
Size: 875 354 3297
Fragments arranged by size:
3297 875 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 334 885 2109 2242 2701 2910 334
Size: 551 1224 133 459 209 1950
Fragments arranged by size:
1950 1224 551 459 209 133
TatI w'GTAC_w
Cuts at: 4041 4041
Size: 4526
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4043 4075 4043
Size: 32 4494
Tth111I GACn'n_nGTC
Cuts at: 2323 2323
Size: 4526
XbaI T'CTAG_A
Cuts at: 1557 1557
Size: 4526
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 489 594 2021 489
Size: 105 1427 2994
Fragments arranged by size:
2994 1427 105
XmaI C'CCGG_G
Cuts at: 1546 1546
Size: 4526
XmnI GAAnn'nnTTC
Cuts at: 313 1073 313
Size: 760 3766
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BglI
BmgBI BmtI BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI