Restriction map for
pF1KB5402
(Circular) (Six-base) MAPSORT of: pF1KB5402.seq Check: 8097 from: 1 to: 4265
pF1KB5402 4265 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AccI GT'mk_AC
Cuts at: 473 1303 473
Size: 830 3435
Acc65I G'GTAC_C
Cuts at: 1281 1281
Size: 4265
AclI AA'CG_TT
Cuts at: 4105 4105
Size: 4265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 985 2090 2522 3269 3537 985
Size: 1105 432 747 268 1713
Fragments arranged by size:
1713 1105 747 432 268
AflIII A'CryG_T
Cuts at: 1617 2721 3698 1617
Size: 1104 977 2184
Fragments arranged by size:
2184 1104 977
AgeI A'CCGG_T
Cuts at: 1194 2653 1194
Size: 1459 2806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 711 711
Size: 4265
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 897 929 897
Size: 32 4233
AlwNI CAG_nnn'CTG
Cuts at: 402 1038 1140 1570 3137 402
Size: 636 102 430 1567 1530
Fragments arranged by size:
1567 1530 636 430 102
ApaI G_GGCC'C
Cuts at: 273 273
Size: 4265
ApaLI G'TGCA_C
Cuts at: 3035 3035
Size: 4265
ApoI r'AATT_y
Cuts at: 1269 3618 3874 1269
Size: 2349 256 1660
Fragments arranged by size:
2349 1660 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4265
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
AvaI C'yCGr_G
Cuts at: 272 1285 272
Size: 1013 3252
BamHI G'GATC_C
Cuts at: 788 1290 788
Size: 502 3763
BanI G'GyrC_C
Cuts at: 131 1159 1191 1197 1281 1695 1943 1978 131
Size: 1028 32 6 84 414 248 35 2418
Fragments arranged by size:
2418 1028 414 248 84 35 32 6
BanII G_rGCy'C
Cuts at: 273 576 1147 1226 1279 2309 273
Size: 303 571 79 53 1030 2229
Fragments arranged by size:
2229 1030 571 303 79 53
BbeI G_GCGC'C
Cuts at: 135 1699 1947 135
Size: 1564 248 2453
Fragments arranged by size:
2453 1564 248
BbsI GAAGACnn'nnnn_
Cuts at: 806 952 806
Size: 146 4119
BciVI GTATCCnnnnn_n'
Cuts at: 2163 2924 4265 2163
Size: 761 1341 2163
Fragments arranged by size:
2163 1341 761
BclI T'GATC_A
Cuts at: 1785 1785
Size: 4265
BfrBI ATG'CAT
Cuts at: 688 688
Size: 4265
BglII A'GATC_T
Cuts at: 1780 3817 1780
Size: 2037 2228
BlpI GC'TnA_GC
Cuts at: 1225 1377 1225
Size: 152 4113
Bme1580I G_kGCm'C
Cuts at: 273 1890 1983 3039 273
Size: 1617 93 1056 1499
Fragments arranged by size:
1617 1499 1056 93
BmgBI CAC'GTC
Cuts at: 119 119
Size: 4265
BmrI ACTGGGnnnn_n'
Cuts at: 637 1477 1558 1893 4037 637
Size: 840 81 335 2144 865
Fragments arranged by size:
2144 865 840 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2632 2632
Size: 4265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1441 2320 2812 3110 1441
Size: 879 492 298 2596
Fragments arranged by size:
2596 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1011 3969 1011
Size: 2958 1307
BsaAI yAC'GTr
Cuts at: 300 1618 300
Size: 1318 2947
BsaBI GATnn'nnATC
Cuts at: 6 1779 3816 6
Size: 1773 2037 455
Fragments arranged by size:
2037 1773 455
BsaHI Gr'CG_yC
Cuts at: 132 353 1696 1944 2646 4142 132
Size: 221 1343 248 702 1496 255
Fragments arranged by size:
1496 1343 702 255 248 221
BsaWI w'CCGG_w
Cuts at: 1194 1454 1975 2653 2927 3074 3485 1194
Size: 260 521 678 274 147 411 1974
Fragments arranged by size:
1974 678 521 411 274 260 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 808 838 866 896 808
Size: 30 28 30 4177
Fragments arranged by size:
4177 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 424 1008 1011 424
Size: 584 3 3678
Fragments arranged by size:
3678 584 3
BseYI C'CCAG_C
Cuts at: 901 1079 1690 2448 3025 3521 901
Size: 178 611 758 577 496 1645
Fragments arranged by size:
1645 758 611 577 496 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 1152 700
Size: 452 3813
BsiEI CG_ry'CG
Cuts at: 104 362 1807 1853 3061 104
Size: 258 1445 46 1208 1308
Fragments arranged by size:
1445 1308 1208 258 46
BsiHKAI G_wGCw'C
Cuts at: 185 1226 1279 2057 2247 3039 185
Size: 1041 53 778 190 792 1411
Fragments arranged by size:
1411 1041 792 778 190 53
Bsp1286I G_dGCh'C
Cuts at: 185 273 576 1147 1226 1279 1890 1983 2057
Size: 88 303 571 79 53 611 93 74
Cuts at: 2057 2247 2309 3039 185
Size: 190 62 730 1411
Fragments arranged by size:
1411 730 611 571 303 190 93 88
79 74 62 53
BspEI T'CCGG_A
Cuts at: 3485 3485
Size: 4265
BspHI T'CATG_A
Cuts at: 3441 3441
Size: 4265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 722 1315 1529 1831 2212 722
Size: 593 214 302 381 2775
Fragments arranged by size:
2775 593 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2557 2611 4095 4260 50
Size: 2507 54 1484 165 55
Fragments arranged by size:
2507 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2177 2177
Size: 4265
BsrFI r'CCGG_y
Cuts at: 191 460 1194 2263 2444 2653 191
Size: 269 734 1069 181 209 1803
Fragments arranged by size:
1803 1069 734 269 209 181
BssHII G'CGCG_C
Cuts at: 169 292 319 321 323 169
Size: 123 27 2 2 4111
Fragments arranged by size:
4111 123 27 2 2
BssSI C'ACGA_G
Cuts at: 2536 2894 2536
Size: 358 3907
BstAPI GCAn_nnn'nTGC
Cuts at: 402 1642 402
Size: 1240 3025
BstBI TT'CG_AA
Cuts at: 2626 2626
Size: 4265
BstXI CCAn_nnnn'nTGG
Cuts at: 930 930
Size: 4265
BstYI r'GATC_y
Cuts at: 1 788 905 1055 1290 1757 1780 2115 2361
Size: 787 117 150 235 467 23 335 246
Cuts at: 2361 3373 3459 3471 3817 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 787 467 449 346 335 246 235
150 117 86 23 12
BtgI C'CryG_G
Cuts at: 362 466 362
Size: 104 4161
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2215 2396 2559 2215
Size: 181 163 3921
Fragments arranged by size:
3921 181 163
BtsI GCAGTG_nn'
Cuts at: 1508 1508
Size: 4265
DraI TTT'AAA
Cuts at: 1264 1264
Size: 4265
DrdI GACnn_nn'nnGTC
Cuts at: 1971 2829 1971
Size: 858 3407
EaeI y'GGCC_r
Cuts at: 359 1247 1850 2024 2415 2442 3787 359
Size: 888 603 174 391 27 1345 837
Fragments arranged by size:
1345 888 837 603 391 174 27
EagI C'GGCC_G
Cuts at: 359 1850 359
Size: 1491 2774
EarI CTCTTCn'nnn_
Cuts at: 1119 2288 2498 1119
Size: 1169 210 2886
Fragments arranged by size:
2886 1169 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 949 2473 2783 2929 4075 949
Size: 1524 310 146 1146 1139
Fragments arranged by size:
1524 1146 1139 310 146
EcoICRI GAG'CTC
Cuts at: 1224 1277 1224
Size: 53 4212
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 985 2090 2522 3269 3537 985
Size: 1105 432 747 268 1713
Fragments arranged by size:
1713 1105 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 337 967 337
Size: 630 3635
EcoO109I rG'GnC_Cy
Cuts at: 882 1404 882
Size: 522 3743
EcoRI G'AATT_C
Cuts at: 1269 1269
Size: 4265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 990 2284 990
Size: 1294 2971
FspI TGC'GCA
Cuts at: 142 2046 142
Size: 1904 2361
HaeII r_GCGC'y
Cuts at: 135 419 1699 1947 2969 3948 135
Size: 284 1280 248 1022 979 452
Fragments arranged by size:
1280 1022 979 452 284 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 563 595 808 840 866 898 2107 2137 2139
Size: 32 213 32 26 32 1209 30 2
Cuts at: 2139 2169 563
Size: 30 2659
Fragments arranged by size:
2659 1209 213 32 32 32 30 30
26 2
HincII GTy'rAC
Cuts at: 243 1304 243
Size: 1061 3204
KasI G'GCGC_C
Cuts at: 131 1695 1943 131
Size: 1564 248 2453
Fragments arranged by size:
2453 1564 248
KpnI G_GTAC'C
Cuts at: 1285 1285
Size: 4265
MluI A'CGCG_T
Cuts at: 3698 3698
Size: 4265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2936 3120 2936
Size: 184 4081
MscI TGG'CCA
Cuts at: 2026 2026
Size: 4265
MslI CAynn'nnrTG
Cuts at: 693 928 2254 2381 693
Size: 235 1326 127 2577
Fragments arranged by size:
2577 1326 235 127
MspA1I CmG'CkG
Cuts at: 138 468 1376 1690 2050 3063 3308 138
Size: 330 908 314 360 1013 245 1095
Fragments arranged by size:
1095 1013 908 360 330 314 245
NaeI GCC'GGC
Cuts at: 193 462 2446 193
Size: 269 1984 2012
Fragments arranged by size:
2012 1984 269
NarI GG'CG_CC
Cuts at: 132 1696 1944 132
Size: 1564 248 2453
Fragments arranged by size:
2453 1564 248
NgoMIV G'CCGG_C
Cuts at: 191 460 2444 191
Size: 269 1984 2012
Fragments arranged by size:
2012 1984 269
NsiI A_TGCA'T
Cuts at: 690 690
Size: 4265
NspI r_CATG'y
Cuts at: 1155 1318 1818 2725 1155
Size: 163 500 907 2695
Fragments arranged by size:
2695 907 500 163
PciI A'CATG_T
Cuts at: 2721 2721
Size: 4265
PmeI GTTT'AAAC
Cuts at: 1264 1264
Size: 4265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 4233
PpuMI rG'GwC_Cy
Cuts at: 882 882
Size: 4265
PspOMI G'GGCC_C
Cuts at: 269 269
Size: 4265
PstI C_TGCA'G
Cuts at: 338 1043 1312 1997 338
Size: 705 269 685 2606
Fragments arranged by size:
2606 705 685 269
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4265
PvuII CAG'CTG
Cuts at: 138 1690 2050 138
Size: 1552 360 2353
Fragments arranged by size:
2353 1552 360
RsrII CG'GwC_CG
Cuts at: 2460 2460
Size: 4265
SacI G_AGCT'C
Cuts at: 1226 1279 1226
Size: 53 4212
SacII CC_GC'GG
Cuts at: 469 469
Size: 4265
SalI G'TCGA_C
Cuts at: 1302 1302
Size: 4265
SapI GCTCTTCn'nnn_
Cuts at: 1119 2288 2498 1119
Size: 1169 210 2886
Fragments arranged by size:
2886 1169 210
SbfI CC_TGCA'GG
Cuts at: 338 1312 338
Size: 974 3291
ScaI AGT'ACT
Cuts at: 3782 3782
Size: 4265
SfcI C'TryA_G
Cuts at: 33 334 615 1039 1308 1993 2986 3177 33
Size: 301 281 424 269 685 993 191 1121
Fragments arranged by size:
1121 993 685 424 301 281 269 191
SfoI GGC'GCC
Cuts at: 133 1697 1945 133
Size: 1564 248 2453
Fragments arranged by size:
2453 1564 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
SgrAI Cr'CCGG_yG
Cuts at: 1194 1194
Size: 4265
SmaI CCC'GGG
Cuts at: 274 1287 274
Size: 1013 3252
SmlI C'TyrA_G
Cuts at: 1420 2335 2827 3089 1420
Size: 915 492 262 2596
Fragments arranged by size:
2596 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4265
SphI G_CATG'C
Cuts at: 1318 1818 1318
Size: 500 3765
StuI AGG'CCT
Cuts at: 760 973 760
Size: 213 4052
StyI C'CwwG_G
Cuts at: 669 1095 1399 1753 669
Size: 426 304 354 3181
Fragments arranged by size:
3181 426 354 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 933 1848 1981 2440 2649 933
Size: 915 133 459 209 2549
Fragments arranged by size:
2549 915 459 209 133
TatI w'GTAC_w
Cuts at: 3780 3780
Size: 4265
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 584 616 3782 3814 584
Size: 32 3166 32 1035
Fragments arranged by size:
3166 1035 32 32
Tth111I GACn'n_nGTC
Cuts at: 859 2062 859
Size: 1203 3062
XbaI T'CTAG_A
Cuts at: 1296 1296
Size: 4265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 898 927 1760 898
Size: 29 833 3403
Fragments arranged by size:
3403 833 29
XmaI C'CCGG_G
Cuts at: 272 1285 272
Size: 1013 3252
XmnI GAAnn'nnTTC
Cuts at: 653 653
Size: 4265
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PciI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BbvCI BcgI BglI BmtI BpmI Bpu10I BsiWI
BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspXI PsrI SanDI SexAI SfiI
SnaBI SrfI SspI SwaI XhoI ZraI