(Circular) (Six-base) MAPSORT of: pF1KB5406.seq Check: 9342 from: 1 to: 4106 pF1KB5406 4106 bp With 182 enzymes: SgfI * June 20, 2008 10:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 264 264 Size: 4106 AccI GT'mk_AC Cuts at: 193 1144 193 Size: 951 3155 Acc65I G'GTAC_C Cuts at: 1122 1122 Size: 4106 AclI AA'CG_TT Cuts at: 3946 3946 Size: 4106 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 110 646 1931 2363 3110 3378 110 Size: 536 1285 432 747 268 838 Fragments arranged by size: 1285 838 747 536 432 268 AflIII A'CryG_T Cuts at: 1458 2562 3539 1458 Size: 1104 977 2025 Fragments arranged by size: 2025 1104 977 AgeI A'CCGG_T Cuts at: 2494 2494 Size: 4106 AlwNI CAG_nnn'CTG Cuts at: 1071 1411 2978 1071 Size: 340 1567 2199 Fragments arranged by size: 2199 1567 340 ApaLI G'TGCA_C Cuts at: 2876 2876 Size: 4106 ApoI r'AATT_y Cuts at: 1110 3459 3715 1110 Size: 2349 256 1501 Fragments arranged by size: 2349 1501 256 AseI AT'TA_AT Cuts at: 20 541 20 Size: 521 3585 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4106 AvaI C'yCGr_G Cuts at: 1126 1126 Size: 4106 BamHI G'GATC_C Cuts at: 1131 1131 Size: 4106 BanI G'GyrC_C Cuts at: 703 1122 1536 1784 1819 703 Size: 419 414 248 35 2990 Fragments arranged by size: 2990 419 414 248 35 BanII G_rGCy'C Cuts at: 1120 2150 1120 Size: 1030 3076 BbeI G_GCGC'C Cuts at: 1540 1788 1540 Size: 248 3858 BciVI GTATCCnnnnn_n' Cuts at: 2004 2765 4106 2004 Size: 761 1341 2004 Fragments arranged by size: 2004 1341 761 BclI T'GATC_A Cuts at: 1626 1626 Size: 4106 BglII A'GATC_T Cuts at: 422 1621 3658 422 Size: 1199 2037 870 Fragments arranged by size: 2037 1199 870 BlpI GC'TnA_GC Cuts at: 1218 1218 Size: 4106 Bme1580I G_kGCm'C Cuts at: 1731 1824 2880 1731 Size: 93 1056 2957 Fragments arranged by size: 2957 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1318 1399 1734 3878 1318 Size: 81 335 2144 1546 Fragments arranged by size: 2144 1546 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2473 2473 Size: 4106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 457 982 457 Size: 525 3581 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 146 922 1282 2161 2653 2951 146 Size: 776 360 879 492 298 1301 Fragments arranged by size: 1301 879 776 492 360 298 BsaI GGTCTCn'nnnn_ Cuts at: 160 958 3810 160 Size: 798 2852 456 Fragments arranged by size: 2852 798 456 BsaAI yAC'GTr Cuts at: 1459 1459 Size: 4106 BsaBI GATnn'nnATC Cuts at: 6 1620 3657 6 Size: 1614 2037 455 Fragments arranged by size: 2037 1614 455 BsaHI Gr'CG_yC Cuts at: 1537 1785 2487 3983 1537 Size: 248 702 1496 1660 Fragments arranged by size: 1660 1496 702 248 BsaWI w'CCGG_w Cuts at: 1295 1816 2494 2768 2915 3326 1295 Size: 521 678 274 147 411 2075 Fragments arranged by size: 2075 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 814 844 814 Size: 30 4076 BseYI C'CCAG_C Cuts at: 138 1531 2289 2866 3362 138 Size: 1393 758 577 496 882 Fragments arranged by size: 1393 882 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 376 376 Size: 4106 BsiEI CG_ry'CG Cuts at: 104 1648 1694 2902 104 Size: 1544 46 1208 1308 Fragments arranged by size: 1544 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1079 1120 1898 2088 2880 1079 Size: 41 778 190 792 2305 Fragments arranged by size: 2305 792 778 190 41 BsmI GAATG_Cn' Cuts at: 620 620 Size: 4106 BsmBI CGTCTCn'nnnn_ Cuts at: 106 106 Size: 4106 Bsp1286I G_dGCh'C Cuts at: 1079 1120 1731 1824 1898 2088 2150 2880 1079 Size: 41 611 93 74 190 62 730 2305 Fragments arranged by size: 2305 730 611 190 93 74 62 41 BspEI T'CCGG_A Cuts at: 3326 3326 Size: 4106 BspHI T'CATG_A Cuts at: 203 3282 203 Size: 3079 1027 BspMI ACCTGCnnnn'nnnn_ Cuts at: 264 956 1156 1370 1672 2053 264 Size: 692 200 214 302 381 2317 Fragments arranged by size: 2317 692 381 302 214 200 BsrBI CCG'CTC Cuts at: 50 2398 2452 3936 4101 50 Size: 2348 54 1484 165 55 Fragments arranged by size: 2348 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2018 2018 Size: 4106 BsrFI r'CCGG_y Cuts at: 2104 2285 2494 2104 Size: 181 209 3716 Fragments arranged by size: 3716 209 181 BsrGI T'GTAC_A Cuts at: 599 768 599 Size: 169 3937 BssSI C'ACGA_G Cuts at: 2377 2735 2377 Size: 358 3748 BstAPI GCAn_nnn'nTGC Cuts at: 1483 1483 Size: 4106 BstBI TT'CG_AA Cuts at: 2467 2467 Size: 4106 BstXI CCAn_nnnn'nTGG Cuts at: 684 684 Size: 4106 BstYI r'GATC_y Cuts at: 1 422 554 1131 1598 1621 1956 2202 3214 Size: 421 132 577 467 23 335 246 1012 Cuts at: 3214 3300 3312 3658 1 Size: 86 12 346 449 Fragments arranged by size: 1012 577 467 449 421 346 335 246 132 86 23 12 BstZ17I GTA'TAC Cuts at: 194 194 Size: 4106 BtgI C'CryG_G Cuts at: 107 107 Size: 4106 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 199 2056 2237 2400 199 Size: 1857 181 163 1905 Fragments arranged by size: 1905 1857 181 163 BtsI GCAGTG_nn' Cuts at: 1349 1349 Size: 4106 ClaI AT'CG_AT Cuts at: 912 912 Size: 4106 DraI TTT'AAA Cuts at: 486 1105 486 Size: 619 3487 DrdI GACnn_nn'nnGTC Cuts at: 1812 2670 1812 Size: 858 3248 EaeI y'GGCC_r Cuts at: 1691 1865 2256 2283 3628 1691 Size: 174 391 27 1345 2169 Fragments arranged by size: 2169 1345 391 174 27 EagI C'GGCC_G Cuts at: 1691 1691 Size: 4106 EarI CTCTTCn'nnn_ Cuts at: 540 683 2129 2339 540 Size: 143 1446 210 2307 Fragments arranged by size: 2307 1446 210 143 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2314 2624 2770 3916 2314 Size: 310 146 1146 2504 Fragments arranged by size: 2504 1146 310 146 EcoICRI GAG'CTC Cuts at: 1118 1118 Size: 4106 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 110 457 646 982 1931 2363 3110 3378 110 Size: 347 189 336 949 432 747 268 838 Fragments arranged by size: 949 838 747 432 347 336 268 189 EcoO109I rG'GnC_Cy Cuts at: 1068 1245 1068 Size: 177 3929 EcoRI G'AATT_C Cuts at: 1110 1110 Size: 4106 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2125 2125 Size: 4106 FspI TGC'GCA Cuts at: 1887 1887 Size: 4106 HaeII r_GCGC'y Cuts at: 1540 1788 2810 3789 1540 Size: 248 1022 979 1857 Fragments arranged by size: 1857 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 517 549 568 600 658 690 814 846 1948 Size: 32 19 32 58 32 124 32 1102 Cuts at: 1948 1978 1980 2010 517 Size: 30 2 30 2613 Fragments arranged by size: 2613 1102 124 58 32 32 32 32 30 30 19 2 HincII GTy'rAC Cuts at: 1145 1145 Size: 4106 HindIII A'AGCT_T Cuts at: 284 284 Size: 4106 KasI G'GCGC_C Cuts at: 1536 1784 1536 Size: 248 3858 KpnI G_GTAC'C Cuts at: 1126 1126 Size: 4106 MluI A'CGCG_T Cuts at: 3539 3539 Size: 4106 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 203 2777 2961 203 Size: 2574 184 1348 Fragments arranged by size: 2574 1348 184 MscI TGG'CCA Cuts at: 1867 1867 Size: 4106 MslI CAynn'nnrTG Cuts at: 2095 2222 2095 Size: 127 3979 MspA1I CmG'CkG Cuts at: 142 1217 1531 1891 2904 3149 142 Size: 1075 314 360 1013 245 1099 Fragments arranged by size: 1099 1075 1013 360 314 245 NaeI GCC'GGC Cuts at: 2287 2287 Size: 4106 NarI GG'CG_CC Cuts at: 1537 1785 1537 Size: 248 3858 NcoI C'CATG_G Cuts at: 107 107 Size: 4106 NgoMIV G'CCGG_C Cuts at: 2285 2285 Size: 4106 NspI r_CATG'y Cuts at: 363 1159 1659 2566 363 Size: 796 500 907 1903 Fragments arranged by size: 1903 907 796 500 PciI A'CATG_T Cuts at: 2562 2562 Size: 4106 PfoI T'CCnGG_A Cuts at: 473 473 Size: 4106 PmeI GTTT'AAAC Cuts at: 1105 1105 Size: 4106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3245 3277 3245 Size: 32 4074 PstI C_TGCA'G Cuts at: 275 1067 1153 1838 275 Size: 792 86 685 2543 Fragments arranged by size: 2543 792 685 86 PvuI CG_AT'CG Cuts at: 104 104 Size: 4106 PvuII CAG'CTG Cuts at: 142 1531 1891 142 Size: 1389 360 2357 Fragments arranged by size: 2357 1389 360 RsrII CG'GwC_CG Cuts at: 2301 2301 Size: 4106 SacI G_AGCT'C Cuts at: 1120 1120 Size: 4106 SalI G'TCGA_C Cuts at: 1143 1143 Size: 4106 SapI GCTCTTCn'nnn_ Cuts at: 683 2129 2339 683 Size: 1446 210 2450 Fragments arranged by size: 2450 1446 210 SbfI CC_TGCA'GG Cuts at: 275 1067 1153 275 Size: 792 86 3228 Fragments arranged by size: 3228 792 86 ScaI AGT'ACT Cuts at: 3623 3623 Size: 4106 SfcI C'TryA_G Cuts at: 33 271 694 1063 1149 1834 2827 3018 33 Size: 238 423 369 86 685 993 191 1121 Fragments arranged by size: 1121 993 685 423 369 238 191 86 SfoI GGC'GCC Cuts at: 1538 1786 1538 Size: 248 3858 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4106 SmaI CCC'GGG Cuts at: 1128 1128 Size: 4106 SmlI C'TyrA_G Cuts at: 161 937 1261 2176 2668 2930 161 Size: 776 324 915 492 262 1337 Fragments arranged by size: 1337 915 776 492 324 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4106 SphI G_CATG'C Cuts at: 363 1159 1659 363 Size: 796 500 2810 Fragments arranged by size: 2810 796 500 SspI AAT'ATT Cuts at: 717 717 Size: 4106 StyI C'CwwG_G Cuts at: 107 413 1240 1594 107 Size: 306 827 354 2619 Fragments arranged by size: 2619 827 354 306 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 625 1689 1822 2281 2490 625 Size: 1064 133 459 209 2241 Fragments arranged by size: 2241 1064 459 209 133 TatI w'GTAC_w Cuts at: 599 768 3621 599 Size: 169 2853 1084 Fragments arranged by size: 2853 1084 169 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3623 3655 3623 Size: 32 4074 Tth111I GACn'n_nGTC Cuts at: 1903 1903 Size: 4106 XbaI T'CTAG_A Cuts at: 1137 1137 Size: 4106 XcmI CCAnnnn_n'nnnnTGG Cuts at: 720 1601 720 Size: 881 3225 XmaI C'CCGG_G Cuts at: 1126 1126 Size: 4106 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BseRI BsiWI BssHII BstEII Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI