Restriction map for
pF1KB5407
(Circular) (Six-base) MAPSORT of: pF1KB5407.seq Check: 2878 from: 1 to: 4595
pF1KB5407 4595 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 198 720 198
Size: 522 4073
AccI GT'mk_AC
Cuts at: 1633 1633
Size: 4595
Acc65I G'GTAC_C
Cuts at: 1611 1611
Size: 4595
AclI AA'CG_TT
Cuts at: 4435 4435
Size: 4595
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1531 2420 2852 3599 3867 1531
Size: 889 432 747 268 2259
Fragments arranged by size:
2259 889 747 432 268
AflIII A'CryG_T
Cuts at: 1947 3051 4028 1947
Size: 1104 977 2514
Fragments arranged by size:
2514 1104 977
AgeI A'CCGG_T
Cuts at: 2983 2983
Size: 4595
AleI CACnn'nnGTG
Cuts at: 932 932
Size: 4595
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 950 950
Size: 4595
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 454 486 454
Size: 32 4563
AlwNI CAG_nnn'CTG
Cuts at: 213 735 981 1474 1900 3467 213
Size: 522 246 493 426 1567 1341
Fragments arranged by size:
1567 1341 522 493 426 246
ApaLI G'TGCA_C
Cuts at: 3365 3365
Size: 4595
ApoI r'AATT_y
Cuts at: 1599 3948 4204 1599
Size: 2349 256 1990
Fragments arranged by size:
2349 1990 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4595
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4595
AvaI C'yCGr_G
Cuts at: 631 721 1029 1615 631
Size: 90 308 586 3611
Fragments arranged by size:
3611 586 308 90
BamHI G'GATC_C
Cuts at: 1620 1620
Size: 4595
BanI G'GyrC_C
Cuts at: 590 712 934 1488 1611 2025 2273 2308 590
Size: 122 222 554 123 414 248 35 2877
Fragments arranged by size:
2877 554 414 248 222 123 122 35
BanII G_rGCy'C
Cuts at: 157 263 1441 1609 2639 157
Size: 106 1178 168 1030 2113
Fragments arranged by size:
2113 1178 1030 168 106
BbeI G_GCGC'C
Cuts at: 594 1492 2029 2277 594
Size: 898 537 248 2912
Fragments arranged by size:
2912 898 537 248
BbsI GAAGACnn'nnnn_
Cuts at: 1110 1110
Size: 4595
BciVI GTATCCnnnnn_n'
Cuts at: 2493 3254 4595 2493
Size: 761 1341 2493
Fragments arranged by size:
2493 1341 761
BclI T'GATC_A
Cuts at: 2115 2115
Size: 4595
BglI GCCn_nnn'nGGC
Cuts at: 976 976
Size: 4595
BglII A'GATC_T
Cuts at: 2110 4147 2110
Size: 2037 2558
BlpI GC'TnA_GC
Cuts at: 1707 1707
Size: 4595
Bme1580I G_kGCm'C
Cuts at: 646 715 939 2220 2313 3369 646
Size: 69 224 1281 93 1056 1872
Fragments arranged by size:
1872 1281 1056 224 93 69
BmgBI CAC'GTC
Cuts at: 885 885
Size: 4595
BmrI ACTGGGnnnn_n'
Cuts at: 1087 1176 1807 1888 2223 4367 1087
Size: 89 631 81 335 2144 1315
Fragments arranged by size:
2144 1315 631 335 89 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2962 2962
Size: 4595
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 643 793 832 1384 1392 1408 1451 1561
Size: 102 150 39 552 8 16 43 110
Cuts at: 1561 541
Size: 3575
Fragments arranged by size:
3575 552 150 110 102 43 39 16
8
Bpu10I CC'TnA_GC
Cuts at: 499 499
Size: 4595
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 1042 1771 2650 3142 3440 451
Size: 591 729 879 492 298 1606
Fragments arranged by size:
1606 879 729 591 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 238 1153 1360 4299 238
Size: 915 207 2939 534
Fragments arranged by size:
2939 915 534 207
BsaAI yAC'GTr
Cuts at: 909 1948 909
Size: 1039 3556
BsaBI GATnn'nnATC
Cuts at: 6 2109 4146 6
Size: 2103 2037 455
Fragments arranged by size:
2103 2037 455
BsaHI Gr'CG_yC
Cuts at: 359 591 740 1489 2026 2274 2976 4472 359
Size: 232 149 749 537 248 702 1496 482
Fragments arranged by size:
1496 749 702 537 482 248 232 149
BsaWI w'CCGG_w
Cuts at: 1784 2305 2983 3257 3404 3815 1784
Size: 521 678 274 147 411 2564
Fragments arranged by size:
2564 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 775 805 775
Size: 30 4565
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 529 538 1017 1567 217
Size: 312 9 479 550 3245
Fragments arranged by size:
3245 550 479 312 9
BseYI C'CCAG_C
Cuts at: 277 1251 2020 2778 3355 3851 277
Size: 974 769 758 577 496 1021
Fragments arranged by size:
1021 974 769 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 835 982 994 562
Size: 273 147 12 4163
Fragments arranged by size:
4163 273 147 12
BsiEI CG_ry'CG
Cuts at: 104 578 2137 2183 3391 104
Size: 474 1559 46 1208 1308
Fragments arranged by size:
1559 1308 1208 474 46
BsiHKAI G_wGCw'C
Cuts at: 157 263 668 764 1609 2387 2577 3369 157
Size: 106 405 96 845 778 190 792 1383
Fragments arranged by size:
1383 845 792 778 405 190 106 96
BsmBI CGTCTCn'nnnn_
Cuts at: 693 778 693
Size: 85 4510
Bsp1286I G_dGCh'C
Cuts at: 157 263 646 668 715 764 939 1441 1609
Size: 106 383 22 47 49 175 502 168
Cuts at: 1609 2220 2313 2387 2577 2639 3369 157
Size: 611 93 74 190 62 730 1383
Fragments arranged by size:
1383 730 611 502 383 190 175 168
106 93 74 62 49 47 22
BspEI T'CCGG_A
Cuts at: 3815 3815
Size: 4595
BspHI T'CATG_A
Cuts at: 869 3771 869
Size: 2902 1693
BspMI ACCTGCnnnn'nnnn_
Cuts at: 198 720 827 1645 1859 2161 2542 198
Size: 522 107 818 214 302 381 2251
Fragments arranged by size:
2251 818 522 381 302 214 107
BsrBI CCG'CTC
Cuts at: 50 2887 2941 4425 4590 50
Size: 2837 54 1484 165 55
Fragments arranged by size:
2837 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2507 2507
Size: 4595
BsrFI r'CCGG_y
Cuts at: 1505 2593 2774 2983 1505
Size: 1088 181 209 3117
Fragments arranged by size:
3117 1088 209 181
BssHII G'CGCG_C
Cuts at: 532 1000 532
Size: 468 4127
BssSI C'ACGA_G
Cuts at: 1381 2866 3224 1381
Size: 1485 358 2752
Fragments arranged by size:
2752 1485 358
BstAPI GCAn_nnn'nTGC
Cuts at: 972 981 1972 972
Size: 9 991 3595
Fragments arranged by size:
3595 991 9
BstBI TT'CG_AA
Cuts at: 2956 2956
Size: 4595
BstYI r'GATC_y
Cuts at: 1 1620 2087 2110 2445 2691 3703 3789 3801
Size: 1619 467 23 335 246 1012 86 12
Cuts at: 3801 4147 1
Size: 346 449
Fragments arranged by size:
1619 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 695 1139 1370 107
Size: 588 444 231 3332
Fragments arranged by size:
3332 588 444 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 981 1278 1284 2545 2726 2889 981
Size: 297 6 1261 181 163 2687
Fragments arranged by size:
2687 1261 297 181 163 6
BtsI GCAGTG_nn'
Cuts at: 1109 1452 1838 1109
Size: 343 386 3866
Fragments arranged by size:
3866 386 343
DraI TTT'AAA
Cuts at: 1594 1594
Size: 4595
DrdI GACnn_nn'nnGTC
Cuts at: 2301 3159 2301
Size: 858 3737
EaeI y'GGCC_r
Cuts at: 575 659 890 1268 1373 2180 2354 2745 2772
Size: 84 231 378 105 807 174 391 27
Cuts at: 2772 4117 575
Size: 1345 1053
Fragments arranged by size:
1345 1053 807 391 378 231 174 105
84 27
EagI C'GGCC_G
Cuts at: 575 2180 575
Size: 1605 2990
EarI CTCTTCn'nnn_
Cuts at: 835 2618 2828 835
Size: 1783 210 2602
Fragments arranged by size:
2602 1783 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 271 2803 3113 3259 4405 271
Size: 2532 310 146 1146 461
Fragments arranged by size:
2532 1146 461 310 146
EcoICRI GAG'CTC
Cuts at: 155 261 1607 155
Size: 106 1346 3143
Fragments arranged by size:
3143 1346 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 643 793 832 1384 1392 1408 1451 1531
Size: 102 150 39 552 8 16 43 80
Cuts at: 1531 1561 2420 2852 3599 3867 541
Size: 30 859 432 747 268 1269
Fragments arranged by size:
1269 859 747 552 432 268 150 102
80 43 39 30 16 8
EcoNI CCTnn'n_nnAGG
Cuts at: 1549 1549
Size: 4595
EcoO109I rG'GnC_Cy
Cuts at: 222 351 1568 1734 222
Size: 129 1217 166 3083
Fragments arranged by size:
3083 1217 166 129
EcoRI G'AATT_C
Cuts at: 1599 1599
Size: 4595
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 870 1083 2614 870
Size: 213 1531 2851
Fragments arranged by size:
2851 1531 213
FspI TGC'GCA
Cuts at: 994 2376 994
Size: 1382 3213
FspAI rTGC'GCAy
Cuts at: 994 994
Size: 4595
HaeII r_GCGC'y
Cuts at: 594 982 1492 2029 2277 3299 4278 594
Size: 388 510 537 248 1022 979 911
Fragments arranged by size:
1022 979 911 537 510 388 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2437 2467 2469 2499 2437
Size: 30 2 30 4533
Fragments arranged by size:
4533 30 30 2
HincII GTy'rAC
Cuts at: 189 1634 189
Size: 1445 3150
KasI G'GCGC_C
Cuts at: 590 1488 2025 2273 590
Size: 898 537 248 2912
Fragments arranged by size:
2912 898 537 248
KpnI G_GTAC'C
Cuts at: 1615 1615
Size: 4595
MluI A'CGCG_T
Cuts at: 4028 4028
Size: 4595
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3266 3450 3266
Size: 184 4411
MscI TGG'CCA
Cuts at: 892 2356 892
Size: 1464 3131
MslI CAynn'nnrTG
Cuts at: 379 706 932 1207 2584 2711 379
Size: 327 226 275 1377 127 2263
Fragments arranged by size:
2263 1377 327 275 226 127
MspA1I CmG'CkG
Cuts at: 160 587 596 1372 1415 1706 2020 2380 3393
Size: 427 9 776 43 291 314 360 1013
Cuts at: 3393 3638 160
Size: 245 1117
Fragments arranged by size:
1117 1013 776 427 360 314 291 245
43 9
NaeI GCC'GGC
Cuts at: 1507 2776 1507
Size: 1269 3326
NarI GG'CG_CC
Cuts at: 591 1489 2026 2274 591
Size: 898 537 248 2912
Fragments arranged by size:
2912 898 537 248
NcoI C'CATG_G
Cuts at: 107 1139 107
Size: 1032 3563
NgoMIV G'CCGG_C
Cuts at: 1505 2774 1505
Size: 1269 3326
NspI r_CATG'y
Cuts at: 1648 2148 3055 1648
Size: 500 907 3188
Fragments arranged by size:
3188 907 500
PasI CC'CwG_GG
Cuts at: 1128 1128
Size: 4595
PciI A'CATG_T
Cuts at: 3051 3051
Size: 4595
PfoI T'CCnGG_A
Cuts at: 134 473 551 620 1361 134
Size: 339 78 69 741 3368
Fragments arranged by size:
3368 741 339 78 69
PmeI GTTT'AAAC
Cuts at: 1594 1594
Size: 4595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 3734
Size: 32 4563
PpuMI rG'GwC_Cy
Cuts at: 222 351 1568 222
Size: 129 1217 3249
Fragments arranged by size:
3249 1217 129
PshAI GACnn'nnGTC
Cuts at: 1216 1216
Size: 4595
PstI C_TGCA'G
Cuts at: 731 1040 1501 1642 2327 731
Size: 309 461 141 685 2999
Fragments arranged by size:
2999 685 461 309 141
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4595
PvuII CAG'CTG
Cuts at: 2020 2380 2020
Size: 360 4235
RsrII CG'GwC_CG
Cuts at: 2790 2790
Size: 4595
SacI G_AGCT'C
Cuts at: 157 263 1609 157
Size: 106 1346 3143
Fragments arranged by size:
3143 1346 106
SacII CC_GC'GG
Cuts at: 1373 1373
Size: 4595
SalI G'TCGA_C
Cuts at: 1632 1632
Size: 4595
SanDI GG'GwC_CC
Cuts at: 351 351
Size: 4595
SapI GCTCTTCn'nnn_
Cuts at: 2618 2828 2618
Size: 210 4385
SbfI CC_TGCA'GG
Cuts at: 1642 1642
Size: 4595
ScaI AGT'ACT
Cuts at: 4112 4112
Size: 4595
SfcI C'TryA_G
Cuts at: 33 727 1036 1497 1638 2323 3316 3507 33
Size: 694 309 461 141 685 993 191 1121
Fragments arranged by size:
1121 993 694 685 461 309 191 141
SfoI GGC'GCC
Cuts at: 592 1490 2027 2275 592
Size: 898 537 248 2912
Fragments arranged by size:
2912 898 537 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4595
SmaI CCC'GGG
Cuts at: 1617 1617
Size: 4595
SmlI C'TyrA_G
Cuts at: 466 1057 1750 2665 3157 3419 466
Size: 591 693 915 492 262 1642
Fragments arranged by size:
1642 915 693 591 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4595
SphI G_CATG'C
Cuts at: 1648 2148 1648
Size: 500 4095
StyI C'CwwG_G
Cuts at: 107 1139 1729 2083 107
Size: 1032 590 354 2619
Fragments arranged by size:
2619 1032 590 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 855 922 1564 2178 2311 2770 2979 855
Size: 67 642 614 133 459 209 2471
Fragments arranged by size:
2471 642 614 459 209 133 67
TatI w'GTAC_w
Cuts at: 383 4110 383
Size: 3727 868
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 775 807 4112 4144 775
Size: 32 3305 32 1226
Fragments arranged by size:
3305 1226 32 32
Tth111I GACn'n_nGTC
Cuts at: 2392 2392
Size: 4595
XbaI T'CTAG_A
Cuts at: 1626 1626
Size: 4595
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1248 1383 2090 1248
Size: 135 707 3753
Fragments arranged by size:
3753 707 135
XmaI C'CCGG_G
Cuts at: 1615 1615
Size: 4595
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BsiWI BsmI BsrGI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PsiI PspOMI PspXI PsrI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI