Restriction map for
pF1KB5414
(Circular) (Six-base) MAPSORT of: pF1KB5414.seq Check: 6297 from: 1 to: 4136
pF1KB5414 4136 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 603 603
Size: 4136
AccI GT'mk_AC
Cuts at: 1174 1174
Size: 4136
Acc65I G'GTAC_C
Cuts at: 697 1152 697
Size: 455 3681
AclI AA'CG_TT
Cuts at: 3976 3976
Size: 4136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 596 965 1961 2393 3140 3408 572
Size: 24 369 996 432 747 268 1300
Fragments arranged by size:
1300 996 747 432 369 268 24
AflII C'TTAA_G
Cuts at: 1102 1102
Size: 4136
AflIII A'CryG_T
Cuts at: 1488 2592 3569 1488
Size: 1104 977 2055
Fragments arranged by size:
2055 1104 977
AgeI A'CCGG_T
Cuts at: 2524 2524
Size: 4136
AlwNI CAG_nnn'CTG
Cuts at: 166 595 717 1441 3008 166
Size: 429 122 724 1567 1294
Fragments arranged by size:
1567 1294 724 429 122
ApaLI G'TGCA_C
Cuts at: 2906 2906
Size: 4136
ApoI r'AATT_y
Cuts at: 485 515 1140 3489 3745 485
Size: 30 625 2349 256 876
Fragments arranged by size:
2349 876 625 256 30
AseI AT'TA_AT
Cuts at: 20 20
Size: 4136
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
AvaI C'yCGr_G
Cuts at: 998 1156 998
Size: 158 3978
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 681 714 681
Size: 33 4103
BamHI G'GATC_C
Cuts at: 1161 1161
Size: 4136
BanI G'GyrC_C
Cuts at: 615 697 1152 1566 1814 1849 615
Size: 82 455 414 248 35 2902
Fragments arranged by size:
2902 455 414 248 82 35
BanII G_rGCy'C
Cuts at: 1150 2180 1150
Size: 1030 3106
BbeI G_GCGC'C
Cuts at: 1570 1818 1570
Size: 248 3888
BbsI GAAGACnn'nnnn_
Cuts at: 362 362
Size: 4136
BciVI GTATCCnnnnn_n'
Cuts at: 2034 2795 4136 2034
Size: 761 1341 2034
Fragments arranged by size:
2034 1341 761
BclI T'GATC_A
Cuts at: 1656 1656
Size: 4136
BglI GCCn_nnn'nGGC
Cuts at: 288 288
Size: 4136
BglII A'GATC_T
Cuts at: 803 1651 3688 803
Size: 848 2037 1251
Fragments arranged by size:
2037 1251 848
BlpI GC'TnA_GC
Cuts at: 1248 1248
Size: 4136
Bme1580I G_kGCm'C
Cuts at: 815 1761 1854 2910 815
Size: 946 93 1056 2041
Fragments arranged by size:
2041 1056 946 93
BmrI ACTGGGnnnn_n'
Cuts at: 1348 1429 1764 3908 1348
Size: 81 335 2144 1576
Fragments arranged by size:
2144 1576 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2503 2503
Size: 4136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 866 920 143
Size: 723 54 3359
Fragments arranged by size:
3359 723 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1312 2191 2683 2981 1312
Size: 879 492 298 2467
Fragments arranged by size:
2467 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3840 3840
Size: 4136
BsaAI yAC'GTr
Cuts at: 1489 1489
Size: 4136
BsaBI GATnn'nnATC
Cuts at: 6 1650 3687 6
Size: 1644 2037 455
Fragments arranged by size:
2037 1644 455
BsaHI Gr'CG_yC
Cuts at: 1567 1815 2517 4013 1567
Size: 248 702 1496 1690
Fragments arranged by size:
1690 1496 702 248
BsaWI w'CCGG_w
Cuts at: 400 1325 1846 2524 2798 2945 3356 400
Size: 925 521 678 274 147 411 1180
Fragments arranged by size:
1180 925 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 595 686 1102 595
Size: 91 416 3629
Fragments arranged by size:
3629 416 91
BseYI C'CCAG_C
Cuts at: 194 326 814 921 1561 2319 2896 3392 194
Size: 132 488 107 640 758 577 496 938
Fragments arranged by size:
938 758 640 577 496 488 132 107
BsiEI CG_ry'CG
Cuts at: 104 1678 1724 2932 104
Size: 1574 46 1208 1308
Fragments arranged by size:
1574 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1150 1928 2118 2910 1150
Size: 778 190 792 2376
Fragments arranged by size:
2376 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 842 842
Size: 4136
Bsp1286I G_dGCh'C
Cuts at: 815 1150 1761 1854 1928 2118 2180 2910 815
Size: 335 611 93 74 190 62 730 2041
Fragments arranged by size:
2041 730 611 335 190 93 74 62
BspEI T'CCGG_A
Cuts at: 400 3356 400
Size: 2956 1180
BspHI T'CATG_A
Cuts at: 3312 3312
Size: 4136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 603 1186 1400 1702 2083 603
Size: 583 214 302 381 2656
Fragments arranged by size:
2656 583 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2428 2482 3966 4131 50
Size: 2378 54 1484 165 55
Fragments arranged by size:
2378 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2048 2048
Size: 4136
BsrFI r'CCGG_y
Cuts at: 2134 2315 2524 2134
Size: 181 209 3746
Fragments arranged by size:
3746 209 181
BssSI C'ACGA_G
Cuts at: 2407 2765 2407
Size: 358 3778
BstAPI GCAn_nnn'nTGC
Cuts at: 241 1513 241
Size: 1272 2864
BstBI TT'CG_AA
Cuts at: 2497 2497
Size: 4136
BstXI CCAn_nnnn'nTGG
Cuts at: 114 142 114
Size: 28 4108
BstYI r'GATC_y
Cuts at: 1 803 1161 1628 1651 1986 2232 3244 3330
Size: 802 358 467 23 335 246 1012 86
Cuts at: 3330 3342 3688 1
Size: 12 346 449
Fragments arranged by size:
1012 802 467 449 358 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 113 306 113
Size: 193 3943
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2086 2267 2430 2086
Size: 181 163 3792
Fragments arranged by size:
3792 181 163
BtsI GCAGTG_nn'
Cuts at: 648 1061 1379 648
Size: 413 318 3405
Fragments arranged by size:
3405 413 318
DraI TTT'AAA
Cuts at: 1135 1135
Size: 4136
DrdI GACnn_nn'nnGTC
Cuts at: 1842 2700 1842
Size: 858 3278
EaeI y'GGCC_r
Cuts at: 1721 1895 2286 2313 3658 1721
Size: 174 391 27 1345 2199
Fragments arranged by size:
2199 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1721 1721
Size: 4136
EarI CTCTTCn'nnn_
Cuts at: 560 572 2159 2369 560
Size: 12 1587 210 2327
Fragments arranged by size:
2327 1587 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2344 2654 2800 3946 2344
Size: 310 146 1146 2534
Fragments arranged by size:
2534 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1148 1148
Size: 4136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 572 596 866 920 965 1961 2393 3140
Size: 429 24 270 54 45 996 432 747
Cuts at: 3140 3408 143
Size: 268 871
Fragments arranged by size:
996 871 747 432 429 270 268 54
45 24
EcoO109I rG'GnC_Cy
Cuts at: 714 1107 1275 714
Size: 393 168 3575
Fragments arranged by size:
3575 393 168
EcoRI G'AATT_C
Cuts at: 1140 1140
Size: 4136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 518 2155 518
Size: 1637 2499
FspI TGC'GCA
Cuts at: 1917 1917
Size: 4136
HaeII r_GCGC'y
Cuts at: 1570 1818 2840 3819 1570
Size: 248 1022 979 1887
Fragments arranged by size:
1887 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 788 820 1978 2008 2010 2040 788
Size: 32 1158 30 2 30 2884
Fragments arranged by size:
2884 1158 32 30 30 2
HincII GTy'rAC
Cuts at: 1175 1175
Size: 4136
KasI G'GCGC_C
Cuts at: 1566 1814 1566
Size: 248 3888
KpnI G_GTAC'C
Cuts at: 701 1156 701
Size: 455 3681
MluI A'CGCG_T
Cuts at: 3569 3569
Size: 4136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2807 2991 2807
Size: 184 3952
MscI TGG'CCA
Cuts at: 1897 1897
Size: 4136
MslI CAynn'nnrTG
Cuts at: 112 2125 2252 112
Size: 2013 127 1996
Fragments arranged by size:
2013 1996 127
MspA1I CmG'CkG
Cuts at: 154 279 684 1247 1561 1921 2934 3179 154
Size: 125 405 563 314 360 1013 245 1111
Fragments arranged by size:
1111 1013 563 405 360 314 245 125
NaeI GCC'GGC
Cuts at: 2317 2317
Size: 4136
NarI GG'CG_CC
Cuts at: 1567 1815 1567
Size: 248 3888
NcoI C'CATG_G
Cuts at: 113 306 113
Size: 193 3943
NgoMIV G'CCGG_C
Cuts at: 2315 2315
Size: 4136
NspI r_CATG'y
Cuts at: 1189 1689 2596 1189
Size: 500 907 2729
Fragments arranged by size:
2729 907 500
PasI CC'CwG_GG
Cuts at: 666 666
Size: 4136
PciI A'CATG_T
Cuts at: 2592 2592
Size: 4136
PmeI GTTT'AAAC
Cuts at: 1135 1135
Size: 4136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3275 3307 3275
Size: 32 4104
PpuMI rG'GwC_Cy
Cuts at: 1107 1107
Size: 4136
PstI C_TGCA'G
Cuts at: 1183 1868 1183
Size: 685 3451
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4136
PvuII CAG'CTG
Cuts at: 154 279 684 1561 1921 154
Size: 125 405 877 360 2369
Fragments arranged by size:
2369 877 405 360 125
RsrII CG'GwC_CG
Cuts at: 2331 2331
Size: 4136
SacI G_AGCT'C
Cuts at: 1150 1150
Size: 4136
SalI G'TCGA_C
Cuts at: 1173 1173
Size: 4136
SapI GCTCTTCn'nnn_
Cuts at: 2159 2369 2159
Size: 210 3926
SbfI CC_TGCA'GG
Cuts at: 1183 1183
Size: 4136
ScaI AGT'ACT
Cuts at: 3653 3653
Size: 4136
SfcI C'TryA_G
Cuts at: 33 507 772 1179 1864 2857 3048 33
Size: 474 265 407 685 993 191 1121
Fragments arranged by size:
1121 993 685 474 407 265 191
SfoI GGC'GCC
Cuts at: 1568 1816 1568
Size: 248 3888
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
SmaI CCC'GGG
Cuts at: 1158 1158
Size: 4136
SmlI C'TyrA_G
Cuts at: 998 1102 1291 2206 2698 2960 998
Size: 104 189 915 492 262 2174
Fragments arranged by size:
2174 915 492 262 189 104
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4136
SphI G_CATG'C
Cuts at: 1189 1689 1189
Size: 500 3636
StuI AGG'CCT
Cuts at: 501 501
Size: 4136
StyI C'CwwG_G
Cuts at: 113 306 553 1270 1624 113
Size: 193 247 717 354 2625
Fragments arranged by size:
2625 717 354 247 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 894 1719 1852 2311 2520 894
Size: 825 133 459 209 2510
Fragments arranged by size:
2510 825 459 209 133
TatI w'GTAC_w
Cuts at: 273 3651 273
Size: 3378 758
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3653 3685 3653
Size: 32 4104
Tth111I GACn'n_nGTC
Cuts at: 1933 1933
Size: 4136
XbaI T'CTAG_A
Cuts at: 1167 1167
Size: 4136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 640 1631 640
Size: 991 3145
XhoI C'TCGA_G
Cuts at: 998 998
Size: 4136
XmaI C'CCGG_G
Cuts at: 1156 1156
Size: 4136
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI ApaI AscI
AvrII BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI
BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI