Restriction map for
pF1KB5418
(Circular) (Six-base) MAPSORT of: pF1KB5418.seq Check: 5244 from: 1 to: 4565
pF1KB5418 4565 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 315 663 1079 1603 315
Size: 348 416 524 3277
Fragments arranged by size:
3277 524 416 348
Acc65I G'GTAC_C
Cuts at: 1581 1581
Size: 4565
AclI AA'CG_TT
Cuts at: 4405 4405
Size: 4565
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1172 1435 1567 2390 2822 3175 3837 1172
Size: 263 132 823 432 353 662 1900
Fragments arranged by size:
1900 823 662 432 353 263 132
AfeI AGC'GCT
Cuts at: 235 235
Size: 4565
AflIII A'CryG_T
Cuts at: 666 1917 3717 3998 666
Size: 1251 1800 281 1233
Fragments arranged by size:
1800 1251 1233 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 117 149 117
Size: 32 4533
AlwNI CAG_nnn'CTG
Cuts at: 1870 3308 1870
Size: 1438 3127
ApaLI G'TGCA_C
Cuts at: 3403 3403
Size: 4565
ApoI r'AATT_y
Cuts at: 936 1420 1569 3918 4174 936
Size: 484 149 2349 256 1327
Fragments arranged by size:
2349 1327 484 256 149
AseI AT'TA_AT
Cuts at: 20 20
Size: 4565
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4565
AvaI C'yCGr_G
Cuts at: 1585 1585
Size: 4565
AvrII C'CTAG_G
Cuts at: 1167 1167
Size: 4565
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1109 1142 1109
Size: 33 4532
BamHI G'GATC_C
Cuts at: 1590 1590
Size: 4565
BanI G'GyrC_C
Cuts at: 1581 1995 2243 2278 1581
Size: 414 248 35 3868
Fragments arranged by size:
3868 414 248 35
BanII G_rGCy'C
Cuts at: 1579 2609 1579
Size: 1030 3535
BbeI G_GCGC'C
Cuts at: 1999 2247 1999
Size: 248 4317
BbsI GAAGACnn'nnnn_
Cuts at: 410 410
Size: 4565
BciVI GTATCCnnnnn_n'
Cuts at: 2463 3519 4565 2463
Size: 1056 1046 2463
Fragments arranged by size:
2463 1056 1046
BclI T'GATC_A
Cuts at: 2085 2085
Size: 4565
BfrBI ATG'CAT
Cuts at: 1208 1208
Size: 4565
BglII A'GATC_T
Cuts at: 2080 4117 2080
Size: 2037 2528
BlpI GC'TnA_GC
Cuts at: 1677 1677
Size: 4565
Bme1580I G_kGCm'C
Cuts at: 222 2190 2283 3407 222
Size: 1968 93 1124 1380
Fragments arranged by size:
1968 1380 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1371 1777 1858 2193 4337 1371
Size: 406 81 335 2144 1599
Fragments arranged by size:
2144 1599 406 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2932 2932
Size: 4565
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1215 1215
Size: 4565
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 1418 1741 2620 3334 3632 335
Size: 1083 323 879 714 298 1268
Fragments arranged by size:
1268 1083 879 714 323 298
BsaI GGTCTCn'nnnn_
Cuts at: 374 4269 374
Size: 3895 670
BsaAI yAC'GTr
Cuts at: 1918 1918
Size: 4565
BsaBI GATnn'nnATC
Cuts at: 6 2079 4116 6
Size: 2073 2037 455
Fragments arranged by size:
2073 2037 455
BsaHI Gr'CG_yC
Cuts at: 1996 2244 2946 4442 1996
Size: 248 702 1496 2119
Fragments arranged by size:
2119 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1754 2275 2953 3364 3511 3785 1754
Size: 521 678 411 147 274 2534
Fragments arranged by size:
2534 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 189 556 189
Size: 367 4198
BseYI C'CCAG_C
Cuts at: 1990 2748 3413 3821 1990
Size: 758 665 408 2734
Fragments arranged by size:
2734 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1018
Size: 4565
BsiEI CG_ry'CG
Cuts at: 104 1353 2107 2153 3383 104
Size: 1249 754 46 1230 1286
Fragments arranged by size:
1286 1249 1230 754 46
BsiHKAI G_wGCw'C
Cuts at: 1149 1579 2357 2547 3407 1149
Size: 430 778 190 860 2307
Fragments arranged by size:
2307 860 778 430 190
BsmI GAATG_Cn'
Cuts at: 1208 1208
Size: 4565
BsmBI CGTCTCn'nnnn_
Cuts at: 563 1459 563
Size: 896 3669
Bsp1286I G_dGCh'C
Cuts at: 222 1149 1579 2190 2283 2357 2547 2609 3407
Size: 927 430 611 93 74 190 62 798
Cuts at: 3407 222
Size: 1380
Fragments arranged by size:
1380 927 798 611 430 190 93 74
62
BspHI T'CATG_A
Cuts at: 2997 2997
Size: 4565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 275 1615 1829 2131 2512 275
Size: 1340 214 302 381 2328
Fragments arranged by size:
2328 1340 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2857 2911 4395 4560 50
Size: 2807 54 1484 165 55
Fragments arranged by size:
2807 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1280 2477 1280
Size: 1197 3368
BsrFI r'CCGG_y
Cuts at: 392 1381 2563 2744 392
Size: 989 1182 181 2213
Fragments arranged by size:
2213 1182 989 181
BssSI C'ACGA_G
Cuts at: 2836 3544 2836
Size: 708 3857
BstAPI GCAn_nnn'nTGC
Cuts at: 1205 1942 1205
Size: 737 3828
BstBI TT'CG_AA
Cuts at: 2926 2926
Size: 4565
BstEII G'GTnAC_C
Cuts at: 547 547
Size: 4565
BstYI r'GATC_y
Cuts at: 1 1590 2057 2080 2415 2661 2967 2979 3065
Size: 1589 467 23 335 246 306 12 86
Cuts at: 3065 4117 1
Size: 1052 449
Fragments arranged by size:
1589 1052 467 449 335 306 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 355 2515 2696 2859 355
Size: 2160 181 163 2061
Fragments arranged by size:
2160 2061 181 163
BtsI GCAGTG_nn'
Cuts at: 222 1808 222
Size: 1586 2979
ClaI AT'CG_AT
Cuts at: 644 1353 644
Size: 709 3856
DraI TTT'AAA
Cuts at: 1564 1564
Size: 4565
DrdI GACnn_nn'nnGTC
Cuts at: 2271 3615 2271
Size: 1344 3221
EaeI y'GGCC_r
Cuts at: 2150 2324 2715 2742 4087 2150
Size: 174 391 27 1345 2628
Fragments arranged by size:
2628 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2150 2150
Size: 4565
EarI CTCTTCn'nnn_
Cuts at: 196 493 2588 2798 196
Size: 297 2095 210 1963
Fragments arranged by size:
2095 1963 297 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2773 3515 3661 4375 2773
Size: 742 146 714 2963
Fragments arranged by size:
2963 742 714 146
EcoICRI GAG'CTC
Cuts at: 1577 1577
Size: 4565
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1172 1215 1435 1567 2390 2822 3175 3837 1172
Size: 43 220 132 823 432 353 662 1900
Fragments arranged by size:
1900 823 662 432 353 220 132 43
EcoO109I rG'GnC_Cy
Cuts at: 1704 1704
Size: 4565
EcoRI G'AATT_C
Cuts at: 1569 1569
Size: 4565
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2584 2584
Size: 4565
FspI TGC'GCA
Cuts at: 2346 2346
Size: 4565
HaeII r_GCGC'y
Cuts at: 237 1999 2247 3477 4248 237
Size: 1762 248 1230 771 554
Fragments arranged by size:
1762 1230 771 554 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 900 932 2407 2437 2439 2469 900
Size: 32 1475 30 2 30 2996
Fragments arranged by size:
2996 1475 32 30 30 2
HincII GTy'rAC
Cuts at: 316 959 1604 316
Size: 643 645 3277
Fragments arranged by size:
3277 645 643
KasI G'GCGC_C
Cuts at: 1995 2243 1995
Size: 248 4317
KpnI G_GTAC'C
Cuts at: 1585 1585
Size: 4565
MluI A'CGCG_T
Cuts at: 3998 3998
Size: 4565
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 770 1270 1460 3324 3508 770
Size: 500 190 1864 184 1827
Fragments arranged by size:
1864 1827 500 190 184
MscI TGG'CCA
Cuts at: 2326 2326
Size: 4565
MslI CAynn'nnrTG
Cuts at: 215 2554 2681 215
Size: 2339 127 2099
Fragments arranged by size:
2339 2099 127
MspA1I CmG'CkG
Cuts at: 776 1676 1990 2350 3134 3379 776
Size: 900 314 360 784 245 1962
Fragments arranged by size:
1962 900 784 360 314 245
NaeI GCC'GGC
Cuts at: 1383 2746 1383
Size: 1363 3202
NarI GG'CG_CC
Cuts at: 1996 2244 1996
Size: 248 4317
NdeI CA'TA_TG
Cuts at: 1098 1310 1098
Size: 212 4353
NgoMIV G'CCGG_C
Cuts at: 1381 2744 1381
Size: 1363 3202
NsiI A_TGCA'T
Cuts at: 1210 1210
Size: 4565
NspI r_CATG'y
Cuts at: 670 1618 2118 3721 670
Size: 948 500 1603 1514
Fragments arranged by size:
1603 1514 948 500
PciI A'CATG_T
Cuts at: 666 3717 666
Size: 3051 1514
PflMI CCAn_nnn'nTGG
Cuts at: 276 789 276
Size: 513 4052
PmeI GTTT'AAAC
Cuts at: 1564 1564
Size: 4565
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 4533
PsiI TTA'TAA
Cuts at: 446 446
Size: 4565
PstI C_TGCA'G
Cuts at: 1612 2297 1612
Size: 685 3880
PvuI CG_AT'CG
Cuts at: 104 1353 104
Size: 1249 3316
PvuII CAG'CTG
Cuts at: 1990 2350 1990
Size: 360 4205
RsrII CG'GwC_CG
Cuts at: 2760 2760
Size: 4565
SacI G_AGCT'C
Cuts at: 1579 1579
Size: 4565
SalI G'TCGA_C
Cuts at: 314 1602 314
Size: 1288 3277
SapI GCTCTTCn'nnn_
Cuts at: 2588 2798 2588
Size: 210 4355
SbfI CC_TGCA'GG
Cuts at: 1612 1612
Size: 4565
ScaI AGT'ACT
Cuts at: 474 768 849 4082 474
Size: 294 81 3233 957
Fragments arranged by size:
3233 957 294 81
SfcI C'TryA_G
Cuts at: 33 1608 2293 3261 3452 33
Size: 1575 685 968 191 1146
Fragments arranged by size:
1575 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1997 2245 1997
Size: 248 4317
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4565
SmaI CCC'GGG
Cuts at: 1587 1587
Size: 4565
SmlI C'TyrA_G
Cuts at: 350 1433 1720 2635 3349 3611 350
Size: 1083 287 915 714 262 1304
Fragments arranged by size:
1304 1083 915 714 287 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4565
SphI G_CATG'C
Cuts at: 1618 2118 1618
Size: 500 4065
SspI AAT'ATT
Cuts at: 1339 1339
Size: 4565
StyI C'CwwG_G
Cuts at: 1167 1699 2053 1167
Size: 532 354 3679
Fragments arranged by size:
3679 532 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2148 2281 2740 2949 2148
Size: 133 459 209 3764
Fragments arranged by size:
3764 459 209 133
TatI w'GTAC_w
Cuts at: 332 472 766 847 1117 4080 332
Size: 140 294 81 270 2963 817
Fragments arranged by size:
2963 817 294 270 140 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 617 649 4082 4114 617
Size: 32 3433 32 1068
Fragments arranged by size:
3433 1068 32 32
Tth111I GACn'n_nGTC
Cuts at: 2362 2362
Size: 4565
XbaI T'CTAG_A
Cuts at: 1596 1596
Size: 4565
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1190 2060 1190
Size: 870 3695
XmaI C'CCGG_G
Cuts at: 1585 1585
Size: 4565
XmnI GAAnn'nnTTC
Cuts at: 1286 1286
Size: 4565
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI ApaI
AscI BbvCI BcgI BglI BmgBI BmtI Bpu10I BsaXI
BsiWI BspEI BsrGI BssHII BstXI BstZ17I Bsu36I BtgI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NheI NotI NruI PacI PasI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI