Restriction map for
pF1KB5432
(Circular) (Six-base) MAPSORT of: pF1KB5432.seq Check: 5843 from: 1 to: 4940
pF1KB5432 4940 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1470 1470
Size: 4940
AccI GT'mk_AC
Cuts at: 1978 1978
Size: 4940
Acc65I G'GTAC_C
Cuts at: 373 1956 373
Size: 1583 3357
AclI AA'CG_TT
Cuts at: 495 4780 495
Size: 4285 655
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 910 1195 1551 1736 1819 1897 1909 2765
Size: 168 285 356 185 83 78 12 856
Cuts at: 2765 3197 3550 4212 742
Size: 432 353 662 1470
Fragments arranged by size:
1470 856 662 432 356 353 285 185
168 83 78 12
AflIII A'CryG_T
Cuts at: 2292 4092 4373 2292
Size: 1800 281 2859
Fragments arranged by size:
2859 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1314 1314
Size: 4940
AlwNI CAG_nnn'CTG
Cuts at: 429 1902 2245 3683 429
Size: 1473 343 1438 1686
Fragments arranged by size:
1686 1473 1438 343
ApaLI G'TGCA_C
Cuts at: 3778 3778
Size: 4940
ApoI r'AATT_y
Cuts at: 390 399 452 1379 1944 4293 4549 390
Size: 9 53 927 565 2349 256 781
Fragments arranged by size:
2349 927 781 565 256 53 9
AseI AT'TA_AT
Cuts at: 20 20
Size: 4940
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4940
AvaI C'yCGr_G
Cuts at: 1960 1960
Size: 4940
BamHI G'GATC_C
Cuts at: 1965 1965
Size: 4940
BanI G'GyrC_C
Cuts at: 373 1347 1956 2370 2618 2653 373
Size: 974 609 414 248 35 2660
Fragments arranged by size:
2660 974 609 414 248 35
BanII G_rGCy'C
Cuts at: 1243 1666 1954 2984 1243
Size: 423 288 1030 3199
Fragments arranged by size:
3199 1030 423 288
BbeI G_GCGC'C
Cuts at: 2374 2622 2374
Size: 248 4692
BbsI GAAGACnn'nnnn_
Cuts at: 629 833 1807 629
Size: 204 974 3762
Fragments arranged by size:
3762 974 204
BciVI GTATCCnnnnn_n'
Cuts at: 316 2838 3894 4940 316
Size: 2522 1056 1046 316
Fragments arranged by size:
2522 1056 1046 316
BclI T'GATC_A
Cuts at: 2460 2460
Size: 4940
BglI GCCn_nnn'nGGC
Cuts at: 244 661 1864 244
Size: 417 1203 3320
Fragments arranged by size:
3320 1203 417
BglII A'GATC_T
Cuts at: 348 1067 2455 4492 348
Size: 719 1388 2037 796
Fragments arranged by size:
2037 1388 796 719
BlpI GC'TnA_GC
Cuts at: 2052 2052
Size: 4940
Bme1580I G_kGCm'C
Cuts at: 1844 2565 2658 3782 1844
Size: 721 93 1124 3002
Fragments arranged by size:
3002 1124 721 93
BmrI ACTGGGnnnn_n'
Cuts at: 1669 2152 2233 2568 4712 1669
Size: 483 81 335 2144 1897
Fragments arranged by size:
2144 1897 483 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3307 3307
Size: 4940
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1297 1379 1457 1597 1114
Size: 183 82 78 140 4457
Fragments arranged by size:
4457 183 140 82 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 2116 2995 3709 4007 934
Size: 1182 879 714 298 1867
Fragments arranged by size:
1867 1182 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 850 1067 4644 850
Size: 217 3577 1146
Fragments arranged by size:
3577 1146 217
BsaAI yAC'GTr
Cuts at: 2293 2293
Size: 4940
BsaBI GATnn'nnATC
Cuts at: 6 347 1709 2454 4491 6
Size: 341 1362 745 2037 455
Fragments arranged by size:
2037 1362 745 455 341
BsaHI Gr'CG_yC
Cuts at: 2371 2619 3321 4817 2371
Size: 248 702 1496 2494
Fragments arranged by size:
2494 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2129 2650 3328 3739 3886 4160 2129
Size: 521 678 411 147 274 2909
Fragments arranged by size:
2909 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 947 1234 1382 1807 947
Size: 287 148 425 4080
Fragments arranged by size:
4080 425 287 148
BseYI C'CCAG_C
Cuts at: 1824 2365 3123 3788 4196 1824
Size: 541 758 665 408 2568
Fragments arranged by size:
2568 758 665 541 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1320 1320
Size: 4940
BsiEI CG_ry'CG
Cuts at: 104 2482 2528 3758 104
Size: 2378 46 1230 1286
Fragments arranged by size:
2378 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 873 1243 1260 1954 2732 2922 3782 873
Size: 370 17 694 778 190 860 2031
Fragments arranged by size:
2031 860 778 694 370 190 17
Bsp1286I G_dGCh'C
Cuts at: 873 1243 1260 1666 1844 1954 2565 2658 2732
Size: 370 17 406 178 110 611 93 74
Cuts at: 2732 2922 2984 3782 873
Size: 190 62 798 2031
Fragments arranged by size:
2031 798 611 406 370 190 178 110
93 74 62 17
BspHI T'CATG_A
Cuts at: 3372 3372
Size: 4940
BspMI ACCTGCnnnn'nnnn_
Cuts at: 869 1470 1990 2204 2506 2887 869
Size: 601 520 214 302 381 2922
Fragments arranged by size:
2922 601 520 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3232 3286 4770 4935 50
Size: 3182 54 1484 165 55
Fragments arranged by size:
3182 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 645 1627 1652 2852 645
Size: 982 25 1200 2733
Fragments arranged by size:
2733 1200 982 25
BsrFI r'CCGG_y
Cuts at: 676 2938 3119 676
Size: 2262 181 2497
Fragments arranged by size:
2497 2262 181
BsrGI T'GTAC_A
Cuts at: 317 317
Size: 4940
BssSI C'ACGA_G
Cuts at: 3211 3919 3211
Size: 708 4232
BstAPI GCAn_nnn'nTGC
Cuts at: 1902 2317 1902
Size: 415 4525
BstBI TT'CG_AA
Cuts at: 3301 3301
Size: 4940
BstYI r'GATC_y
Cuts at: 1 348 520 1067 1965 2432 2455 2790 3036
Size: 347 172 547 898 467 23 335 246
Cuts at: 3036 3342 3354 3440 4492 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 898 547 467 449 347 335 306
246 172 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 793 1192 793
Size: 399 4541
BtgI C'CryG_G
Cuts at: 516 633 516
Size: 117 4823
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 158 2890 3071 3234 158
Size: 2732 181 163 1864
Fragments arranged by size:
2732 1864 181 163
BtsI GCAGTG_nn'
Cuts at: 345 1622 2183 345
Size: 1277 561 3102
Fragments arranged by size:
3102 1277 561
DraI TTT'AAA
Cuts at: 405 1939 405
Size: 1534 3406
DraIII CAC_nnn'GTG
Cuts at: 538 538
Size: 4940
DrdI GACnn_nn'nnGTC
Cuts at: 2646 3990 2646
Size: 1344 3596
EaeI y'GGCC_r
Cuts at: 2525 2699 3090 3117 4462 2525
Size: 174 391 27 1345 3003
Fragments arranged by size:
3003 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2525 2525
Size: 4940
EarI CTCTTCn'nnn_
Cuts at: 164 204 1106 1355 1784 1873 2963 3173 164
Size: 40 902 249 429 89 1090 210 1931
Fragments arranged by size:
1931 1090 902 429 249 210 89 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3148 3890 4036 4750 3148
Size: 742 146 714 3338
Fragments arranged by size:
3338 742 714 146
EcoICRI GAG'CTC
Cuts at: 1241 1952 1241
Size: 711 4229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 910 1114 1195 1297 1379 1457 1551 1597
Size: 168 204 81 102 82 78 94 46
Cuts at: 1597 1736 1819 1897 1909 2765 3197 3550 4212
Size: 139 83 78 12 856 432 353 662
Cuts at: 4212 742
Size: 1470
Fragments arranged by size:
1470 856 662 432 353 204 168 139
102 94 83 82 81 78 78 46
12
EcoO109I rG'GnC_Cy
Cuts at: 789 1196 2079 789
Size: 407 883 3650
Fragments arranged by size:
3650 883 407
EcoRI G'AATT_C
Cuts at: 452 1944 452
Size: 1492 3448
EcoRV GAT'ATC
Cuts at: 765 765
Size: 4940
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 193 1464 1533 2959 193
Size: 1271 69 1426 2174
Fragments arranged by size:
2174 1426 1271 69
FspI TGC'GCA
Cuts at: 2721 2721
Size: 4940
HaeII r_GCGC'y
Cuts at: 2374 2622 3852 4623 2374
Size: 248 1230 771 2691
Fragments arranged by size:
2691 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 752 784 2782 2812 2814 2844 752
Size: 32 1998 30 2 30 2848
Fragments arranged by size:
2848 1998 32 30 30 2
HincII GTy'rAC
Cuts at: 278 1979 278
Size: 1701 3239
HindIII A'AGCT_T
Cuts at: 1510 1510
Size: 4940
KasI G'GCGC_C
Cuts at: 2370 2618 2370
Size: 248 4692
KpnI G_GTAC'C
Cuts at: 377 1960 377
Size: 1583 3357
MluI A'CGCG_T
Cuts at: 4373 4373
Size: 4940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1346 3699 3883 1346
Size: 2353 184 2403
Fragments arranged by size:
2403 2353 184
MscI TGG'CCA
Cuts at: 2701 2701
Size: 4940
MslI CAynn'nnrTG
Cuts at: 638 1745 2929 3056 638
Size: 1107 1184 127 2522
Fragments arranged by size:
2522 1184 1107 127
MspA1I CmG'CkG
Cuts at: 1407 2051 2365 2725 3509 3754 1407
Size: 644 314 360 784 245 2593
Fragments arranged by size:
2593 784 644 360 314 245
NaeI GCC'GGC
Cuts at: 3121 3121
Size: 4940
NarI GG'CG_CC
Cuts at: 2371 2619 2371
Size: 248 4692
NcoI C'CATG_G
Cuts at: 633 633
Size: 4940
NgoMIV G'CCGG_C
Cuts at: 3119 3119
Size: 4940
NspI r_CATG'y
Cuts at: 1993 2493 4096 1993
Size: 500 1603 2837
Fragments arranged by size:
2837 1603 500
PciI A'CATG_T
Cuts at: 4092 4092
Size: 4940
PfoI T'CCnGG_A
Cuts at: 1928 1928
Size: 4940
PmeI GTTT'AAAC
Cuts at: 1939 1939
Size: 4940
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3386 3418 3386
Size: 32 4908
PpuMI rG'GwC_Cy
Cuts at: 789 789
Size: 4940
PsiI TTA'TAA
Cuts at: 929 929
Size: 4940
PstI C_TGCA'G
Cuts at: 1559 1987 2672 1559
Size: 428 685 3827
Fragments arranged by size:
3827 685 428
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4940
PvuII CAG'CTG
Cuts at: 1407 2365 2725 1407
Size: 958 360 3622
Fragments arranged by size:
3622 958 360
RsrII CG'GwC_CG
Cuts at: 3135 3135
Size: 4940
SacI G_AGCT'C
Cuts at: 1243 1954 1243
Size: 711 4229
SalI G'TCGA_C
Cuts at: 1977 1977
Size: 4940
SapI GCTCTTCn'nnn_
Cuts at: 1106 2963 3173 1106
Size: 1857 210 2873
Fragments arranged by size:
2873 1857 210
SbfI CC_TGCA'GG
Cuts at: 1987 1987
Size: 4940
ScaI AGT'ACT
Cuts at: 4457 4457
Size: 4940
SfcI C'TryA_G
Cuts at: 33 756 1555 1983 2668 3636 3827 33
Size: 723 799 428 685 968 191 1146
Fragments arranged by size:
1146 968 799 723 685 428 191
SfoI GGC'GCC
Cuts at: 2372 2620 2372
Size: 248 4692
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4940
SmaI CCC'GGG
Cuts at: 1962 1962
Size: 4940
SmlI C'TyrA_G
Cuts at: 949 2095 3010 3724 3986 949
Size: 1146 915 714 262 1903
Fragments arranged by size:
1903 1146 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4940
SphI G_CATG'C
Cuts at: 1993 2493 1993
Size: 500 4440
SspI AAT'ATT
Cuts at: 202 411 202
Size: 209 4731
StuI AGG'CCT
Cuts at: 1582 1858 1582
Size: 276 4664
StyI C'CwwG_G
Cuts at: 242 633 851 1583 1589 1634 1667 1772 2074
Size: 391 218 732 6 45 33 105 302
Cuts at: 2074 2428 242
Size: 354 2754
Fragments arranged by size:
2754 732 391 354 302 218 105 45
33 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2523 2656 3115 3324 2523
Size: 133 459 209 4139
Fragments arranged by size:
4139 459 209 133
TatI w'GTAC_w
Cuts at: 317 1024 4455 317
Size: 707 3431 802
Fragments arranged by size:
3431 802 707
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4457 4489 4457
Size: 32 4908
Tth111I GACn'n_nGTC
Cuts at: 2737 2737
Size: 4940
XbaI T'CTAG_A
Cuts at: 456 1971 456
Size: 1515 3425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2435 2435
Size: 4940
XmaI C'CCGG_G
Cuts at: 1960 1960
Size: 4940
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BspEI BssHII BstEII
BstXI BstZ17I ClaI CspCI EcoNI FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI
ZraI